BuamA.00119.a
D-alanine--D-alanine ligase
| CENTER ID: | BuamA.00119.a |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00119.a.A1.GE33135 | Full length( BuamA.00119.a ) | 1 | 313 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00119.a.A1.PS01235 | Full length( BuamA.00119.a ) | 1 | 313 |
Structures
4EG0
DEPOSITED: 3/30/2012
DETERMINATION: XRay
CLONE: BuamA.00119.a.A1.GE33135
PROTEIN: BuamA.00119.a.A1.PS01236
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_130117 |
| PATRIC ID: | fig|398577.6.peg.485 |
| RefSeq: | YP_001807203.1 |
| UniProt: | B1YSS6 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGIDPKRFG KVAVLFGGES AEREVSLTSG RLVLQGLRDA GIDAHPFDPA ERPLSALKDE GFVRAFNALH GGYGENGQIQ GALDFYGIRY TGSGVLGSAL GLDKFRTKLV WQQTGVPTPP FETVMRGDDY AARATDIVAK LGLPLFVKPA SEGSSVAVLK VKTADALPAA LSEAATHDKI VIVEKSIEGG GEYTACIAGD LDLPLIKIVP AGEFYDYHAK YVANDTQYLI PCGLPAEQET ELKRIARRAF DVLGCTDWGR ADFMLDAAGN AYFLEVNTAP GMTDHSLPPK AARSIGIGYS ELVVKVLSLT LND
NT Sequence
ATGAGCGGGA TCGATCCGAA ACGTTTCGGC AAGGTGGCAG TGTTGTTCGG CGGCGAATCC GCCGAGCGCG AGGTGTCGCT CACCTCGGGC CGCCTCGTGC TGCAGGGCCT GCGCGACGCG GGCATCGACG CGCATCCGTT CGACCCGGCC GAGCGGCCGC TGTCGGCGCT GAAGGATGAA GGCTTCGTGC GCGCGTTCAA CGCGCTGCAC GGCGGCTATG GCGAGAACGG CCAGATCCAG GGCGCGCTCG ATTTCTACGG GATTCGCTAC ACGGGCAGCG GCGTGCTCGG CTCGGCGCTT GGTCTCGACA AGTTCCGCAC GAAGCTCGTG TGGCAGCAGA CGGGCGTGCC GACGCCGCCG TTCGAGACGG TGATGCGCGG CGACGACTAC GCGGCGCGCG CGACGGACAT CGTCGCGAAG CTCGGCCTGC CGCTGTTCGT GAAGCCGGCG AGCGAAGGCT CGAGCGTCGC GGTGCTGAAG GTCAAGACGG CCGACGCGTT GCCCGCCGCA CTGTCGGAAG CCGCGACGCA CGACAAGATC GTGATCGTCG AGAAGAGCAT CGAAGGCGGC GGCGAATACA CCGCGTGCAT CGCCGGCGAT CTCGATCTCC CGCTGATCAA GATCGTGCCG GCGGGCGAGT TCTACGACTA CCACGCGAAG TATGTCGCCA ACGATACGCA GTACCTGATT CCGTGCGGGC TGCCGGCGGA GCAGGAAACA GAACTGAAGC GCATCGCGCG CCGCGCGTTC GACGTGCTCG GCTGCACCGA CTGGGGCCGC GCGGATTTCA TGCTCGACGC CGCCGGCAAC GCGTATTTCC TGGAAGTGAA CACGGCCCCC GGGATGACCG ACCATTCGCT GCCGCCGAAG GCCGCGCGCT CGATCGGCAT CGGCTATTCG GAGCTGGTCG TGAAGGTGCT GTCGCTTACG CTCAACGAC
Details for BuamA.00119.a.A1.GE33135
| HARVESTED ON: | 8/19/2011 |
| SEQUENCED ON: | 8/31/2011 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Validated AA Sequence
MSGIDPKRFG KVAVLFGGES AEREVSLTSG RLVLQGLRDA GIDAHPFDPA ERPLSALKDE GFVRAFNALH GGYGENGQIQ GALDFYGIRY TGSGVLGSAL GLDKFRTKLV WQQTGVPTPP FETVMRGDDY AARATDIVAK LGLPLFVKPA SEGSSVAVLK VKTADALPAA LSEAATHDKI VIVEKSIEGG GEYTACIAGD LDLPLIKIVP AGEFYDYHAK YVANDTQYLI PCGLPAEQET ELKRIARRAF DVLGCTDWGR ADFMLDAAGN AYFLEVNTAP GMTDHSLPPK AARSIGIGYS ELVVKVLSLT L
Validated NT Sequence
tgagcgtaag cgacagcacc ttcacgacca gctccgaata gccgatgccg atcgagcgcg cggccttcgg cggcagcgaa tggtcggtca tcccgggggc cgtgttcact tccaggaaat acgcgttgcc ggcggcgtcg agcatgaaat ccgcgcggcc ccagtcggtg cagccgagca cgtcgaacgc gcggcgcgcg atgcgcttca gttctgtttc ctgctccgcc ggcagcccgc acggaatcag gtactgcgta tcgttggcga catacttcgc gtggtagtcg tagaactcgc ccgccggcac gatcttgatc agcgggagat cgagatcgcc ggcgatgcac gcggtgtatt cgccgccgcc ttcgatgctc ttctcgacga tcacgatctt gtcgtgcgtc gcggcttccg acagtgcggc gggcaacgcg tcggccgtct tgaccttcag caccgcgacg ctcgagcctt cgctcgccgg cttcacgaac agcggcaggc cgagcttcgc gacgatgtcc gtcgcgcgcg ccgcgtagtc gtcgccgcgc atcaccgtct cgaacggcgg cgtcggcacg cccgtctgct gccacacgag cttcgtgcgg aacttgtcga gaccaagcgc cgagccgagc acgccgctgc ccgtgtagcg aatcccgtag aaatcgagcg cgccctggat ctggccgttc tcgccatagc cgccgtgcag cgcgttgaac gcgcgcacga agccttcatc cttcagcgcc gacagcggcc gctcggccgg gtcgaacgga tgcgcgtcga tgcccgcgtc gcgcaggccc tgcagcacga ggcggcccga ggtgagcgac acctcgcgct cggcggattc gccgccgaac aacactgcca ccttgccgaa acgtttcgga tcgatcccgc tcatcgaacc aggaccctgg gtctgagctt ccagggtacc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGIDPKRF GKVAVLFGGE SAEREVSLTS GRLVLQGLRD AGIDAHPFDP AERPLSALKD EGFVRAFNAL HGGYGENGQI QGALDFYGIR YTGSGVLGSA LGLDKFRTKL VWQQTGVPTP PFETVMRGDD YAARATDIVA KLGLPLFVKP ASEGSSVAVL KVKTADALPA ALSEAATHDK IVIVEKSIEG GGEYTACIAG DLDLPLIKIV PAGEFYDYHA KYVANDTQYL IPCGLPAEQE TELKRIARRA FDVLGCTDWG RADFMLDAAG NAYFLEVNTA PGMTDHSLPP KAARSIGIGY SELVVKVLSL TLND
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgggatc gatccgaaac gtttcggcaa ggtggcagtg ttgttcggcg gcgaatccgc cgagcgcgag gtgtcgctca cctcgggccg cctcgtgctg cagggcctgc gcgacgcggg catcgacgcg catccgttcg acccggccga gcggccgctg tcggcgctga aggatgaagg cttcgtgcgc gcgttcaacg cgctgcacgg cggctatggc gagaacggcc agatccaggg cgcgctcgat ttctacggga ttcgctacac gggcagcggc gtgctcggct cggcgcttgg tctcgacaag ttccgcacga agctcgtgtg gcagcagacg ggcgtgccga cgccgccgtt cgagacggtg atgcgcggcg acgactacgc ggcgcgcgcg acggacatcg tcgcgaagct cggcctgccg ctgttcgtga agccggcgag cgaaggctcg agcgtcgcgg tgctgaaggt caagacggcc gacgcgttgc ccgccgcact gtcggaagcc gcgacgcacg acaagatcgt gatcgtcgag aagagcatcg aaggcggcgg cgaatacacc gcgtgcatcg ccggcgatct cgatctcccg ctgatcaaga tcgtgccggc gggcgagttc tacgactacc acgcgaagta tgtcgccaac gatacgcagt acctgattcc gtgcgggctg ccggcggagc aggaaacaga actgaagcgc atcgcgcgcc gcgcgttcga cgtgctcggc tgcaccgact ggggccgcgc ggatttcatg ctcgacgccg ccggcaacgc gtatttcctg gaagtgaaca cggcccccgg gatgaccgac cattcgctgc cgccgaaggc cgcgcgctcg atcggcatcg gctattcgga gctggtcgtg aaggtgctgt cgcttacgct caacgactaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa
Details for BuamA.00119.a.A1.PS01235
| PURIFICATION DATe: | 10/13/2011 |
| CONCENTRATION: | 70.65mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 93 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSGIDPKRFG KVAVLFGGES AEREVSLTSG RLVLQGLRDA GIDAHPFDPA ERPLSALKDE GFVRAFNALH GGYGENGQIQ GALDFYGIRY TGSGVLGSAL GLDKFRTKLV WQQTGVPTPP FETVMRGDDY AARATDIVAK LGLPLFVKPA SEGSSVAVLK VKTADALPAA LSEAATHDKI VIVEKSIEGG GEYTACIAGD LDLPLIKIVP AGEFYDYHAK YVANDTQYLI PCGLPAEQET ELKRIARRAF DVLGCTDWGR ADFMLDAAGN AYFLEVNTAP GMTDHSLPPK AARSIGIGYS ELVVKVLSLT L
Validated NT Sequence
tgagcgtaag cgacagcacc ttcacgacca gctccgaata gccgatgccg atcgagcgcg cggccttcgg cggcagcgaa tggtcggtca tcccgggggc cgtgttcact tccaggaaat acgcgttgcc ggcggcgtcg agcatgaaat ccgcgcggcc ccagtcggtg cagccgagca cgtcgaacgc gcggcgcgcg atgcgcttca gttctgtttc ctgctccgcc ggcagcccgc acggaatcag gtactgcgta tcgttggcga catacttcgc gtggtagtcg tagaactcgc ccgccggcac gatcttgatc agcgggagat cgagatcgcc ggcgatgcac gcggtgtatt cgccgccgcc ttcgatgctc ttctcgacga tcacgatctt gtcgtgcgtc gcggcttccg acagtgcggc gggcaacgcg tcggccgtct tgaccttcag caccgcgacg ctcgagcctt cgctcgccgg cttcacgaac agcggcaggc cgagcttcgc gacgatgtcc gtcgcgcgcg ccgcgtagtc gtcgccgcgc atcaccgtct cgaacggcgg cgtcggcacg cccgtctgct gccacacgag cttcgtgcgg aacttgtcga gaccaagcgc cgagccgagc acgccgctgc ccgtgtagcg aatcccgtag aaatcgagcg cgccctggat ctggccgttc tcgccatagc cgccgtgcag cgcgttgaac gcgcgcacga agccttcatc cttcagcgcc gacagcggcc gctcggccgg gtcgaacgga tgcgcgtcga tgcccgcgtc gcgcaggccc tgcagcacga ggcggcccga ggtgagcgac acctcgcgct cggcggattc gccgccgaac aacactgcca ccttgccgaa acgtttcgga tcgatcccgc tcatcgaacc aggaccctgg gtctgagctt ccagggtacc
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSGIDPKRF GKVAVLFGGE SAEREVSLTS GRLVLQGLRD AGIDAHPFDP AERPLSALKD EGFVRAFNAL HGGYGENGQI QGALDFYGIR YTGSGVLGSA LGLDKFRTKL VWQQTGVPTP PFETVMRGDD YAARATDIVA KLGLPLFVKP ASEGSSVAVL KVKTADALPA ALSEAATHDK IVIVEKSIEG GGEYTACIAG DLDLPLIKIV PAGEFYDYHA KYVANDTQYL IPCGLPAEQE TELKRIARRA FDVLGCTDWG RADFMLDAAG NAYFLEVNTA PGMTDHSLPP KAARSIGIGY SELVVKVLSL TLND
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAGCGGGATC GATCCGAAAC GTTTCGGCAA GGTGGCAGTG TTGTTCGGCG GCGAATCCGC CGAGCGCGAG GTGTCGCTCA CCTCGGGCCG CCTCGTGCTG CAGGGCCTGC GCGACGCGGG CATCGACGCG CATCCGTTCG ACCCGGCCGA GCGGCCGCTG TCGGCGCTGA AGGATGAAGG CTTCGTGCGC GCGTTCAACG CGCTGCACGG CGGCTATGGC GAGAACGGCC AGATCCAGGG CGCGCTCGAT TTCTACGGGA TTCGCTACAC GGGCAGCGGC GTGCTCGGCT CGGCGCTTGG TCTCGACAAG TTCCGCACGA AGCTCGTGTG GCAGCAGACG GGCGTGCCGA CGCCGCCGTT CGAGACGGTG ATGCGCGGCG ACGACTACGC GGCGCGCGCG ACGGACATCG TCGCGAAGCT CGGCCTGCCG CTGTTCGTGA AGCCGGCGAG CGAAGGCTCG AGCGTCGCGG TGCTGAAGGT CAAGACGGCC GACGCGTTGC CCGCCGCACT GTCGGAAGCC GCGACGCACG ACAAGATCGT GATCGTCGAG AAGAGCATCG AAGGCGGCGG CGAATACACC GCGTGCATCG CCGGCGATCT CGATCTCCCG CTGATCAAGA TCGTGCCGGC GGGCGAGTTC TACGACTACC ACGCGAAGTA TGTCGCCAAC GATACGCAGT ACCTGATTCC GTGCGGGCTG CCGGCGGAGC AGGAAACAGA ACTGAAGCGC ATCGCGCGCC GCGCGTTCGA CGTGCTCGGC TGCACCGACT GGGGCCGCGC GGATTTCATG CTCGACGCCG CCGGCAACGC GTATTTCCTG GAAGTGAACA CGGCCCCCGG GATGACCGAC CATTCGCTGC CGCCGAAGGC CGCGCGCTCG ATCGGCATCG GCTATTCGGA GCTGGTCGTG AAGGTGCTGT CGCTTACGCT CAACGACTAA ACAGCACGAA CAAGTTCTGC AGCCAAGCTT CTCGAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA