BuamA.00118.c

UTP-glucose-1-phosphate uridylyltransferase

CENTER ID: BuamA.00118.c
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00118.c.B1.GE39787 Full length( BuamA.00118.c ) 1 294
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.6382
RefSeq: YP_001815910.1
UniProt: B1Z3P4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKVTKAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAIDAGI TEMIFVTGRS KRAIEDHFDK SYEIEAELEA RGKEKLLSLV RSIKPSHVDC FYVRQAEALG LGHAVLCAEK LVGNQPFAVI LADDLLDGPT PVLRQMIDVF DHYHASVIGV EEIAPEASKS YGVIAGKRWE DNLFKLSGIV EKPEPAKAPS NFGVVGRYVL KPKVFEHLRA LRLGAGGELQ LTDAIQSLLA DEQVLAHRYE GTRFDCGSKL GYLKATVEFA LRHPEVAADF ESYLATRMSA LLVA
NT Sequence
ATGTTGAAAG TGACGAAAGC CGTATTTCCG GTTGCCGGTC TCGGCACACG ATTTCTGCCC GCGACCAAAG CCAGCCCGAA AGAAATGCTG CCGGTCGTGG ACAAGCCGCT GATTCAATAT GCGGTCGAGG AGGCGATCGA CGCCGGCATC ACCGAAATGA TTTTCGTGAC CGGCCGCAGC AAGCGCGCGA TCGAGGATCA CTTCGACAAG TCCTACGAGA TCGAAGCGGA GCTCGAAGCA CGCGGCAAGG AGAAGCTGCT GTCGCTGGTT CGCAGCATCA AGCCGAGCCA TGTCGACTGC TTCTACGTAC GCCAGGCCGA AGCACTGGGG CTCGGCCATG CCGTGCTGTG CGCGGAGAAG CTGGTCGGTA ACCAGCCGTT CGCGGTGATC CTCGCCGACG ATCTGCTCGA CGGTCCGACG CCCGTGCTGC GCCAGATGAT CGACGTGTTC GATCACTATC ACGCGTCGGT GATCGGCGTG GAGGAAATCG CGCCCGAGGC ATCGAAGTCG TACGGTGTGA TCGCCGGCAA GCGCTGGGAG GACAATTTGT TCAAGTTGTC GGGAATCGTC GAGAAGCCGG AGCCGGCGAA GGCGCCGTCG AATTTCGGCG TGGTGGGCCG TTACGTGCTG AAGCCGAAGG TGTTCGAGCA TCTGCGCGCG CTGCGGCTCG GCGCCGGCGG CGAACTGCAG TTGACGGACG CGATCCAGTC GCTGCTCGCT GACGAGCAGG TGCTCGCCCA TCGCTACGAA GGCACGCGCT TCGATTGCGG CAGCAAGCTC GGGTATTTGA AGGCGACGGT CGAGTTCGCG CTGCGGCATC CGGAAGTGGC CGCGGATTTC GAAAGCTATC TCGCCACGCG CATGTCCGCG CTGCTGGTCG CC
Details for BuamA.00118.c.B1.GE39787
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIDAGITE MIFVTGRSKR AIEDHFDKSY EIEAELEARG KEKLLSLVRS IKPSHVDCFY VRQAEALGLG HAVLCAEKLV GNQPFAVILA DDLLDGPTPV LRQMIDVFDH YHASVIGVEE IAPEASKSYG VIAGKRWEDN LFKLSGIVEK PEPAKAPSNF GVVGRYVLKP KVFEHLRALR LGAGGELQLT DAIQSLLADE QVLAHRYEGT RFDCGSKLGY LKATVEFALR HPEVAADFES YLATXXXALL VA
Validated NT Sequence
ttactcactt aggcgaccag cagcgcggnn atgcncgtgg cgagatagct ttcgaaatcc gcggccactt ccggatgccg cagcgcgaac tcgaccgtcg ccttcaaata cccgagcttg ctgccgcaat cgaagcgcgt gccttcgtag cgatgggcga gcacctgctc gtcagcgagc agcgactgga tcgcgtccgt caactgcagt tcgccgccgg cgccgagccg cagcgcgcgc agatgctcga acaccttcgg cttcagcacg taacggccca ccacgccgaa attcgacggc gccttcgccg gctccggctt ctcgacgatt cccgacaact tgaacaaatt gtcctcccag cgcttgccgg cgatcacacc gtacgacttc gatgcctcgg gcgcgatttc ctccacgccg atcaccgacg cgtgatagtg atcgaacacg tcgatcatct ggcgcagcac gggcgtcgga ccgtcgagca gatcgtcggc gaggatcacc gcgaacggct ggttaccgac cagcttctcc gcgcacagca cggcatggcc gagccccagt gcttcggcct ggcgtacgta gaagcagtcg acatggctcg gcttgatgct gcgaaccagc gacagcagct tctccttgcc gcgtgcttcg agctccgctt cgatctcgta ggacttgtcg aagtgatcct cgatcgcgcg cttgctgcgg ccggtcacga aaatcatttc ggtgatgccg gcgtcgatcg cctcctcgac cgcatattga atcagcggct tgtccacgac cggcagcatt tctttcgggc tggctttggt cgcgggcaga aatcgtgtgc cgagaccggc aaccggaaat acggctttcg tcactttcaa catatggtgg tggtggtggt gagccatggt atatctc
Expected Protein Sequence
MAHHHHHHML KVTKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIDAGITE MIFVTGRSKR AIEDHFDKSY EIEAELEARG KEKLLSLVRS IKPSHVDCFY VRQAEALGLG HAVLCAEKLV GNQPFAVILA DDLLDGPTPV LRQMIDVFDH YHASVIGVEE IAPEASKSYG VIAGKRWEDN LFKLSGIVEK PEPAKAPSNF GVVGRYVLKP KVFEHLRALR LGAGGELQLT DAIQSLLADE QVLAHRYEGT RFDCGSKLGY LKATVEFALR HPEVAADFES YLATRMSALL VA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga aagtgacgaa agccgtattt ccggttgccg gtctcggcac acgatttctg cccgcgacca aagccagccc gaaagaaatg ctgccggtcg tggacaagcc gctgattcaa tatgcggtcg aggaggcgat cgacgccggc atcaccgaaa tgattttcgt gaccggccgc agcaagcgcg cgatcgagga tcacttcgac aagtcctacg agatcgaagc ggagctcgaa gcacgcggca aggagaagct gctgtcgctg gttcgcagca tcaagccgag ccatgtcgac tgcttctacg tacgccaggc cgaagcactg gggctcggcc atgccgtgct gtgcgcggag aagctggtcg gtaaccagcc gttcgcggtg atcctcgccg acgatctgct cgacggtccg acgcccgtgc tgcgccagat gatcgacgtg ttcgatcact atcacgcgtc ggtgatcggc gtggaggaaa tcgcgcccga ggcatcgaag tcgtacggtg tgatcgccgg caagcgctgg gaggacaatt tgttcaagtt gtcgggaatc gtcgagaagc cggagccggc gaaggcgccg tcgaatttcg gcgtggtggg ccgttacgtg ctgaagccga aggtgttcga gcatctgcgc gcgctgcggc tcggcgccgg cggcgaactg cagttgacgg acgcgatcca gtcgctgctc gctgacgagc aggtgctcgc ccatcgctac gaaggcacgc gcttcgattg cggcagcaag ctcgggtatt tgaaggcgac ggtcgagttc gcgctgcggc atccggaagt ggccgcggat ttcgaaagct atctcgccac gcgcatgtcc gcgctgctgg tcgcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat