SSGCID: Target Page

BuamA.00118.b

UTP-glucose-1-phosphate uridylyltransferase

CENTER ID:	BuamA.00118.b
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00118.b.B1.GE39786	Full length( BuamA.00118.b )		1	295

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.3993
RefSeq:	YP_001810553.1
UniProt:	B1Z203

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKVTRAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAINAGI TEMIFVTGRS KRAIEDHFDK SYEIEAELEA RGKEKLLELV RGIKPSHVNC FYVRQPEALG LGHAVLCAEK LVHGEPFAVI LADDLLHGEQ PVLKQLVDVF DHYHSSVIGV ETIPREDSRS YGVVEGREWE EDIIKLSGII EKPAPEDAPS NLGVVGRYVF MPTIFDHLRK LKPGAGGELQ LTDAVQSLLA NEQVLAYRYF GTRFDCGSKI GYLKATVELA LQHPEVSREF EAYLRTCLPA LAAVA

NT Sequence

ATGTTGAAAG TCACCAGGGC CGTGTTTCCC GTTGCCGGTC TCGGCACGCG GTTCCTCCCG GCAACGAAGG CGAGCCCGAA GGAAATGCTG CCCGTCGTCG ACAAGCCGCT GATCCAGTAC GCGGTCGAGG AAGCGATCAA CGCCGGTATT ACCGAGATGA TCTTCGTGAC GGGGCGCAGC AAGCGCGCGA TCGAGGACCA CTTCGACAAG TCCTACGAGA TCGAGGCCGA ACTCGAGGCG CGCGGCAAGG AAAAGCTGCT CGAGCTCGTG CGCGGCATCA AGCCGAGCCA CGTGAACTGC TTCTACGTGC GCCAGCCGGA AGCGCTCGGC CTCGGCCATG CGGTGTTGTG CGCGGAAAAG CTCGTGCACG GCGAGCCGTT CGCGGTGATC CTCGCCGACG ACCTGCTGCA CGGCGAACAG CCGGTGCTCA AGCAGCTCGT CGACGTGTTC GACCACTATC ACAGCTCGGT GATCGGGGTC GAGACGATCC CGCGCGAGGA CAGCCGCTCG TACGGCGTCG TCGAAGGCCG CGAGTGGGAA GAGGACATCA TCAAGCTGTC GGGCATCATC GAGAAGCCGG CGCCGGAAGA CGCGCCGTCG AACCTCGGCG TGGTCGGCCG CTACGTATTC ATGCCGACGA TCTTCGATCA TCTGCGCAAG CTCAAGCCGG GCGCCGGCGG CGAACTGCAG CTGACCGACG CCGTCCAGTC GCTGCTCGCG AACGAACAGG TGCTCGCGTA TCGCTACTTC GGCACGCGTT TCGATTGCGG CAGCAAGATC GGTTATCTCA AGGCGACCGT CGAGCTCGCG CTCCAGCATC CGGAAGTGAG CCGCGAATTC GAGGCGTACC TGCGTACCTG CCTGCCCGCG CTGGCTGCTG TCGCA

Details for BuamA.00118.b.B1.GE39786

HARVESTED ON:	2/4/2016
SEQUENCED ON:	1/27/2016
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML KVTRAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAINAGITE MIFVTGRSKR AIEDHFDKSY EIEAELEARG KEKLLELVRG IKPSHVNCFY VRQPEALGLG HAVLCAEKLV HGEPFAVILA DDLLHGEQPV LKQLVDVFDH YHSSVIGVET IPREDSRSYG VVEGREWEED IIKLSGIIEK PAPEDAPSNL GVVGRYVFMP TIFDHLRKLK PGAGGELQLT DAVQSLLANE QVLAYRYFGT RFDCGSKIGY LKATVELALQ HPEVSREFEA YLRTCXXALA AVA

Validated NT Sequence

ttactcactt atgcgacagc agccagcgcg gnnnngcagg tacgcaggta cgcctcgaat tcgcggctca cttccggatg ctggagcgcg agctcgacgg tcgccttgag ataaccgatc ttgctgccgc aatcgaaacg cgtgccgaag tagcgatacg cgagcacctg ttcgttcgcg agcagcgact ggacggcgtc ggtcagctgc agttcgccgc cggcgcccgg cttgagcttg cgcagatgat cgaagatcgt cggcatgaat acgtagcggc cgaccacgcc gaggttcgac ggcgcgtctt ccggcgccgg cttctcgatg atgcccgaca gcttgatgat gtcctcttcc cactcgcggc cttcgacgac gccgtacgag cggctgtcct cgcgcgggat cgtctcgacc ccgatcaccg agctgtgata gtggtcgaac acgtcgacga gctgcttgag caccggctgt tcgccgtgca gcaggtcgtc ggcgaggatc accgcgaacg gctcgccgtg cacgagcttt tccgcgcaca acaccgcatg gccgaggccg agcgcttccg gctggcgcac gtagaagcag ttcacgtggc tcggcttgat gccgcgcacg agctcgagca gcttttcctt gccgcgcgcc tcgagttcgg cctcgatctc gtaggacttg tcgaagtggt cctcgatcgc gcgcttgctg cgccccgtca cgaagatcat ctcggtaata ccggcgttga tcgcttcctc gaccgcgtac tggatcagcg gcttgtcgac gacgggcagc atttccttcg ggctcgcctt cgttgccggg aggaaccgcg tgccgagacc ggcaacggga aacacggccc tggtgacttt caacatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga aagtcaccag ggccgtgttt cccgttgccg gtctcggcac gcggttcctc ccggcaacga aggcgagccc gaaggaaatg ctgcccgtcg tcgacaagcc gctgatccag tacgcggtcg aggaagcgat caacgccggt attaccgaga tgatcttcgt gacggggcgc agcaagcgcg cgatcgagga ccacttcgac aagtcctacg agatcgaggc cgaactcgag gcgcgcggca aggaaaagct gctcgagctc gtgcgcggca tcaagccgag ccacgtgaac tgcttctacg tgcgccagcc ggaagcgctc ggcctcggcc atgcggtgtt gtgcgcggaa aagctcgtgc acggcgagcc gttcgcggtg atcctcgccg acgacctgct gcacggcgaa cagccggtgc tcaagcagct cgtcgacgtg ttcgaccact atcacagctc ggtgatcggg gtcgagacga tcccgcgcga ggacagccgc tcgtacggcg tcgtcgaagg ccgcgagtgg gaagaggaca tcatcaagct gtcgggcatc atcgagaagc cggcgccgga agacgcgccg tcgaacctcg gcgtggtcgg ccgctacgta ttcatgccga cgatcttcga tcatctgcgc aagctcaagc cgggcgccgg cggcgaactg cagctgaccg acgccgtcca gtcgctgctc gcgaacgaac aggtgctcgc gtatcgctac ttcggcacgc gtttcgattg cggcagcaag atcggttatc tcaaggcgac cgtcgagctc gcgctccagc atccggaagt gagccgcgaa ttcgaggcgt acctgcgtac ctgcctgccc gcgctggctg ctgtcgcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat