BuamA.00118.a
UTP-glucose-1-phosphate uridylyltransferase
| CENTER ID: | BuamA.00118.a |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00118.a.B1.GE39785 | Full length( BuamA.00118.a ) | 1 | 294 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00118.a.B1.PS37963 | Full length( BuamA.00118.a ) | 1 | 294 |
Structures
5VCT
DEPOSITED: 3/31/2017
DETERMINATION: XRay
CLONE: BuamA.00118.a.B1.GE39785
PROTEIN: BuamA.00118.a.B1.PS37963
5VE7
DEPOSITED: 4/4/2017
DETERMINATION: XRay
CLONE: BuamA.00118.a.B1.GE39785
PROTEIN: BuamA.00118.a.B1.PS37963
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_131936 |
| PATRIC ID: | fig|398577.6.peg.1390 |
| RefSeq: | YP_001808063.1 |
| UniProt: | B1YNX3 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLKVNKAVFP VAGLGTRFLP ATKASPKEML PVVDKPLIQY AVEEAIAAGI TEMIFVTGRS KRAIEDHFDK SYEVEAELEA RGKAKLLELV RSIKPSHVDC FYVRQPEALG LGHAVLCAEK LVADNPFAVI LADDLLDGNP PVMKQMVDVF DHYHSSVIGV EEIPPSETKS YGIVDGKEWE ESIVKMSAIV EKPAPEVAPS NLGVVGRYIL KPRIFEHLRA LKPGAGGELQ LTDAIQALLA DEQVLAYKYQ GTRYDCGSKL GYLKATVEFA LRHPEVGTEF DAYLRTRGNA HPAA
NT Sequence
ATGTTGAAAG TCAACAAGGC GGTATTCCCC GTTGCCGGTC TCGGCACGCG GTTCCTCCCT GCAACGAAGG CGAGCCCGAA GGAAATGCTG CCGGTCGTCG ACAAGCCGCT GATCCAGTAC GCGGTCGAGG AAGCGATCGC CGCGGGCATC ACCGAGATGA TCTTCGTGAC CGGGCGCAGC AAGCGTGCGA TCGAGGATCA CTTCGACAAG TCGTACGAGG TCGAGGCCGA ACTCGAGGCG CGCGGCAAGG CAAAGCTGCT CGAGCTCGTG CGCAGCATCA AGCCGAGCCA CGTCGACTGC TTCTACGTGC GCCAGCCGGA AGCGCTCGGC CTCGGCCATG CGGTGCTGTG CGCGGAAAAG CTCGTGGCGG ACAACCCGTT CGCGGTGATC CTCGCCGACG ACCTGCTCGA CGGCAACCCG CCGGTGATGA AGCAGATGGT CGACGTGTTC GACCACTATC ACAGCTCGGT GATCGGCGTG GAAGAGATTC CGCCGTCGGA GACGAAGTCG TACGGGATCG TCGATGGCAA GGAGTGGGAA GAGTCGATCG TCAAGATGTC GGCGATCGTC GAGAAGCCGG CGCCGGAAGT CGCGCCGTCG AACCTCGGCG TGGTCGGCCG CTACATCCTG AAGCCGCGCA TCTTCGAACA CCTGCGTGCG CTGAAGCCGG GCGCGGGCGG CGAGCTGCAG CTCACCGACG CGATCCAGGC GCTGCTCGCC GACGAACAGG TGCTGGCCTA CAAGTACCAG GGCACGCGCT ACGACTGCGG CAGCAAGCTC GGCTACCTGA AGGCGACGGT CGAATTCGCG CTTCGCCACC CGGAAGTCGG CACCGAGTTC GACGCGTACC TGCGCACGCG CGGCAACGCG CATCCGGCCG CC
Details for BuamA.00118.a.B1.GE39785
| HARVESTED ON: | 2/4/2016 |
| SEQUENCED ON: | 1/27/2016 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML KVNKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EVEAELEARG KAKLLELVRS IKPSHVDCFY VRQPEALGLG HAVLCAEKLV ADNPFAVILA DDLLDGNPPV MKQMVDVFDH YHSSVIGVEE IPPSETKSYG IVDGKEWEES IVKMSAIVEK PAPEVAPSNL GVVGRYILKP RIFEHLRALK PGAGGELQLT DAIQALLADE QVLAYKYQGT RYDCGSKLGY LKATVEFALR HPEVGTEFDA YLRTXXNAHP AA
Validated NT Sequence
atcttactca cttaggcggc cggatgcgcg ttnnnnnncg tgcgcaggta cgcgtcgaac tcggtgccga cttccgggtg gcgaagcgcg aattcgaccg tcgccttcag gtagccgagc ttgctgccgc agtcgtagcg cgtgccctgg tacttgtagg ccagcacctg ttcgtcggcg agcagcgcct ggatcgcgtc ggtgagctgc agctcgccgc ccgcgcccgg cttcagcgca cgcaggtgtt cgaagatgcg cggcttcagg atgtagcggc cgaccacgcc gaggttcgac ggcgcgactt ccggcgccgg cttctcgacg atcgccgaca tcttgacgat cgactcttcc cactccttgc catcgacgat cccgtacgac ttcgtctccg acggcggaat ctcttccacg ccgatcaccg agctgtgata gtggtcgaac acgtcgacca tctgcttcat caccggcggg ttgccgtcga gcaggtcgtc ggcgaggatc accgcgaacg ggttgtccgc cacgagcttt tccgcgcaca gcaccgcatg gccgaggccg agcgcttccg gctggcgcac gtagaagcag tcgacgtggc tcggcttgat gctgcgcacg agctcgagca gctttgcctt gccgcgcgcc tcgagttcgg cctcgacctc gtacgacttg tcgaagtgat cctcgatcgc acgcttgctg cgcccggtca cgaagatcat ctcggtgatg cccgcggcga tcgcttcctc gaccgcgtac tggatcagcg gcttgtcgac gaccggcagc atttccttcg ggctcgcctt cgttgcaggg aggaaccgcg tgccgagacc ggcaacgggg aataccgcct tgttgacttt caacatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHML KVNKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EVEAELEARG KAKLLELVRS IKPSHVDCFY VRQPEALGLG HAVLCAEKLV ADNPFAVILA DDLLDGNPPV MKQMVDVFDH YHSSVIGVEE IPPSETKSYG IVDGKEWEES IVKMSAIVEK PAPEVAPSNL GVVGRYILKP RIFEHLRALK PGAGGELQLT DAIQALLADE QVLAYKYQGT RYDCGSKLGY LKATVEFALR HPEVGTEFDA YLRTRGNAHP AA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttga aagtcaacaa ggcggtattc cccgttgccg gtctcggcac gcggttcctc cctgcaacga aggcgagccc gaaggaaatg ctgccggtcg tcgacaagcc gctgatccag tacgcggtcg aggaagcgat cgccgcgggc atcaccgaga tgatcttcgt gaccgggcgc agcaagcgtg cgatcgagga tcacttcgac aagtcgtacg aggtcgaggc cgaactcgag gcgcgcggca aggcaaagct gctcgagctc gtgcgcagca tcaagccgag ccacgtcgac tgcttctacg tgcgccagcc ggaagcgctc ggcctcggcc atgcggtgct gtgcgcggaa aagctcgtgg cggacaaccc gttcgcggtg atcctcgccg acgacctgct cgacggcaac ccgccggtga tgaagcagat ggtcgacgtg ttcgaccact atcacagctc ggtgatcggc gtggaagaga ttccgccgtc ggagacgaag tcgtacggga tcgtcgatgg caaggagtgg gaagagtcga tcgtcaagat gtcggcgatc gtcgagaagc cggcgccgga agtcgcgccg tcgaacctcg gcgtggtcgg ccgctacatc ctgaagccgc gcatcttcga acacctgcgt gcgctgaagc cgggcgcggg cggcgagctg cagctcaccg acgcgatcca ggcgctgctc gccgacgaac aggtgctggc ctacaagtac cagggcacgc gctacgactg cggcagcaag ctcggctacc tgaaggcgac ggtcgaattc gcgcttcgcc acccggaagt cggcaccgag ttcgacgcgt acctgcgcac gcgcggcaac gcgcatccgg ccgcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuamA.00118.a.B1.PS37963
| PURIFICATION DATe: | 6/28/2016 |
| CONCENTRATION: | 45.5mg/ml |
| OBSERVED MW: | 34kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML KVNKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EVEAELEARG KAKLLELVRS IKPSHVDCFY VRQPEALGLG HAVLCAEKLV ADNPFAVILA DDLLDGNPPV MKQMVDVFDH YHSSVIGVEE IPPSETKSYG IVDGKEWEES IVKMSAIVEK PAPEVAPSNL GVVGRYILKP RIFEHLRALK PGAGGELQLT DAIQALLADE QVLAYKYQGT RYDCGSKLGY LKATVEFALR HPEVGTEFDA YLRTXXNAHP AA
Validated NT Sequence
atcttactca cttaggcggc cggatgcgcg ttnnnnnncg tgcgcaggta cgcgtcgaac tcggtgccga cttccgggtg gcgaagcgcg aattcgaccg tcgccttcag gtagccgagc ttgctgccgc agtcgtagcg cgtgccctgg tacttgtagg ccagcacctg ttcgtcggcg agcagcgcct ggatcgcgtc ggtgagctgc agctcgccgc ccgcgcccgg cttcagcgca cgcaggtgtt cgaagatgcg cggcttcagg atgtagcggc cgaccacgcc gaggttcgac ggcgcgactt ccggcgccgg cttctcgacg atcgccgaca tcttgacgat cgactcttcc cactccttgc catcgacgat cccgtacgac ttcgtctccg acggcggaat ctcttccacg ccgatcaccg agctgtgata gtggtcgaac acgtcgacca tctgcttcat caccggcggg ttgccgtcga gcaggtcgtc ggcgaggatc accgcgaacg ggttgtccgc cacgagcttt tccgcgcaca gcaccgcatg gccgaggccg agcgcttccg gctggcgcac gtagaagcag tcgacgtggc tcggcttgat gctgcgcacg agctcgagca gctttgcctt gccgcgcgcc tcgagttcgg cctcgacctc gtacgacttg tcgaagtgat cctcgatcgc acgcttgctg cgcccggtca cgaagatcat ctcggtgatg cccgcggcga tcgcttcctc gaccgcgtac tggatcagcg gcttgtcgac gaccggcagc atttccttcg ggctcgcctt cgttgcaggg aggaaccgcg tgccgagacc ggcaacgggg aataccgcct tgttgacttt caacatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHML KVNKAVFPVA GLGTRFLPAT KASPKEMLPV VDKPLIQYAV EEAIAAGITE MIFVTGRSKR AIEDHFDKSY EVEAELEARG KAKLLELVRS IKPSHVDCFY VRQPEALGLG HAVLCAEKLV ADNPFAVILA DDLLDGNPPV MKQMVDVFDH YHSSVIGVEE IPPSETKSYG IVDGKEWEES IVKMSAIVEK PAPEVAPSNL GVVGRYILKP RIFEHLRALK PGAGGELQLT DAIQALLADE QVLAYKYQGT RYDCGSKLGY LKATVEFALR HPEVGTEFDA YLRTRGNAHP AA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGA AAGTCAACAA GGCGGTATTC CCCGTTGCCG GTCTCGGCAC GCGGTTCCTC CCTGCAACGA AGGCGAGCCC GAAGGAAATG CTGCCGGTCG TCGACAAGCC GCTGATCCAG TACGCGGTCG AGGAAGCGAT CGCCGCGGGC ATCACCGAGA TGATCTTCGT GACCGGGCGC AGCAAGCGTG CGATCGAGGA TCACTTCGAC AAGTCGTACG AGGTCGAGGC CGAACTCGAG GCGCGCGGCA AGGCAAAGCT GCTCGAGCTC GTGCGCAGCA TCAAGCCGAG CCACGTCGAC TGCTTCTACG TGCGCCAGCC GGAAGCGCTC GGCCTCGGCC ATGCGGTGCT GTGCGCGGAA AAGCTCGTGG CGGACAACCC GTTCGCGGTG ATCCTCGCCG ACGACCTGCT CGACGGCAAC CCGCCGGTGA TGAAGCAGAT GGTCGACGTG TTCGACCACT ATCACAGCTC GGTGATCGGC GTGGAAGAGA TTCCGCCGTC GGAGACGAAG TCGTACGGGA TCGTCGATGG CAAGGAGTGG GAAGAGTCGA TCGTCAAGAT GTCGGCGATC GTCGAGAAGC CGGCGCCGGA AGTCGCGCCG TCGAACCTCG GCGTGGTCGG CCGCTACATC CTGAAGCCGC GCATCTTCGA ACACCTGCGT GCGCTGAAGC CGGGCGCGGG CGGCGAGCTG CAGCTCACCG ACGCGATCCA GGCGCTGCTC GCCGACGAAC AGGTGCTGGC CTACAAGTAC CAGGGCACGC GCTACGACTG CGGCAGCAAG CTCGGCTACC TGAAGGCGAC GGTCGAATTC GCGCTTCGCC ACCCGGAAGT CGGCACCGAG TTCGACGCGT ACCTGCGCAC GCGCGGCAAC GCGCATCCGG CCGCCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT