BuamA.00117.b
Catechol 1,2-dioxygenase
| CENTER ID: | BuamA.00117.b |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00117.b.B1.GE39784 | Full length( BuamA.00117.b ) | 1 | 317 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
5VXT
DEPOSITED: 5/24/2017
DETERMINATION: XRay
CLONE: BuamA.00117.b.B1.GE39784
PROTEIN: BuamA.00117.b.B1.PS37936
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|398577.6.peg.6779 |
| RefSeq: | YP_001816286.1 |
| UniProt: | B1Z4S0 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSVKVFDTKE VQDLLKAASN AGTNGANGTQ AGNARTQQIV HRLLGDLFKA IDDLDITPDE VWAGVNYLNK LGQDGEAALL AAGLGLEKYL DIRMDAEDEA IGLDGGTPRT IEGPLYVAGA PVRDGVAKID LDADEGAGPL VIHGTVTGLD GKPVAGALVE CWHANSHGFY SHFDPTGKQS DFNLRGAVKT GADGKYEFRT LMPVGYGCPP QGATQQLLDR LGRHGNRPAH VHFFVTSDGH RKLTTQFNIE GDPLIWDDFA YATREELIPP VTAKAGGAAL GLKADAYQDI EFNFVLTPRV EGKDNQIVER LRASATA
NT Sequence
ATGAGCGTCA AAGTTTTCGA TACGAAGGAA GTGCAGGATC TGCTGAAGGC CGCGTCGAAC GCGGGCACGA ACGGCGCGAA CGGCACGCAG GCCGGCAACG CGCGCACGCA GCAGATCGTC CACCGGCTGC TCGGCGACCT GTTCAAGGCG ATCGACGATC TCGACATCAC GCCCGACGAA GTGTGGGCCG GCGTGAACTA CCTGAACAAG CTCGGCCAGG ACGGCGAAGC GGCGCTGCTC GCGGCGGGCC TCGGCCTCGA GAAGTATCTC GACATCCGGA TGGACGCCGA GGACGAGGCG ATCGGCCTGG ACGGCGGCAC GCCGCGCACG ATCGAAGGCC CGCTGTATGT CGCCGGCGCG CCGGTGCGCG ACGGCGTCGC GAAGATCGAT CTCGACGCGG ACGAAGGCGC GGGCCCGCTC GTGATCCACG GCACGGTCAC GGGGCTCGAC GGCAAGCCGG TGGCCGGCGC GCTGGTCGAG TGCTGGCACG CGAACTCGCA CGGCTTCTAT TCGCACTTCG ACCCGACCGG CAAGCAGAGC GATTTCAACC TGCGCGGCGC GGTGAAGACG GGCGCGGACG GCAAGTACGA ATTCCGCACG CTGATGCCGG TCGGCTACGG CTGCCCGCCG CAGGGCGCGA CGCAGCAGCT GCTGGATCGC CTCGGCCGTC ACGGCAACCG TCCGGCGCAC GTGCACTTCT TCGTGACCAG CGACGGCCAC CGCAAGCTGA CGACGCAGTT CAACATCGAA GGCGACCCGC TGATCTGGGA TGACTTCGCG TATGCGACGC GCGAGGAGCT GATTCCGCCC GTCACTGCCA AGGCCGGCGG CGCCGCGCTC GGGCTGAAGG CCGACGCGTA TCAGGACATC GAGTTCAACT TCGTCCTGAC GCCGCGCGTC GAGGGCAAGG ACAACCAGAT CGTCGAACGC CTGCGCGCTT CCGCCACGGC A
Details for BuamA.00117.b.B1.GE39784
| HARVESTED ON: | 2/4/2016 |
| SEQUENCED ON: | 1/27/2016 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 35kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMS VKVFDTKEVQ DLLKAASNAG TNGANGTQAG NARTQQIVHR LLGDLFKAID DLDITPDEVW AGVNYLNKLG QDGEAALLAA GLGLEKYLDI RMDAEDEAIG LDGGTPRTIE GPLYVAGAPV RDGVAKIDLD ADEGAGPLVI HGTVTGLDGK PVAGALVECW HANSHGFYSH FDPTGKQSDF NLRGAVKTGA DGKYEFRTLM PVGYGCPPQG ATQQLLDRLG RHGNRPAHVH FFVTSDGHRK LTTQFNIEGD PLIWDDFAYA TREELIPPVT AKAGGAALGL KADAYQDIEF NFVLTPRVEG KDNQIVXXXR ASATA
Validated NT Sequence
tcttactcac ttatgccgtg gcggaagcgc gnnnnnnntc gacgatctgg ttgtccttgc cctcgacgcg cggcgtcagg acgaagttga actcgatgtc ctgatacgcg tcggccttca gcccgagcgc ggcgccgccg gccttggcag tgacgggcgg aatcagctcc tcgcgcgtcg catacgcgaa gtcatcccag atcagcgggt cgccttcgat gttgaactgc gtcgtcagct tgcggtggcc gtcgctggtc acgaagaagt gcacgtgcgc cggacggttg ccgtgacggc cgaggcgatc cagcagctgc tgcgtcgcgc cctgcggcgg gcagccgtag ccgaccggca tcagcgtgcg gaattcgtac ttgccgtccg cgcccgtctt caccgcgccg cgcaggttga aatcgctctg cttgccggtc gggtcgaagt gcgaatagaa gccgtgcgag ttcgcgtgcc agcactcgac cagcgcgccg gccaccggct tgccgtcgag ccccgtgacc gtgccgtgga tcacgagcgg gcccgcgcct tcgtccgcgt cgagatcgat cttcgcgacg ccgtcgcgca ccggcgcgcc ggcgacatac agcgggcctt cgatcgtgcg cggcgtgccg ccgtccaggc cgatcgcctc gtcctcggcg tccatccgga tgtcgagata cttctcgagg ccgaggcccg ccgcgagcag cgccgcttcg ccgtcctggc cgagcttgtt caggtagttc acgccggccc acacttcgtc gggcgtgatg tcgagatcgt cgatcgcctt gaacaggtcg ccgagcagcc ggtggacgat ctgctgcgtg cgcgcgttgc cggcctgcgt gccgttcgcg ccgttcgtgc ccgcgttcga cgcggccttc agcagatcct gcacttcctt cgtatcgaaa actttgacgc tcatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMS VKVFDTKEVQ DLLKAASNAG TNGANGTQAG NARTQQIVHR LLGDLFKAID DLDITPDEVW AGVNYLNKLG QDGEAALLAA GLGLEKYLDI RMDAEDEAIG LDGGTPRTIE GPLYVAGAPV RDGVAKIDLD ADEGAGPLVI HGTVTGLDGK PVAGALVECW HANSHGFYSH FDPTGKQSDF NLRGAVKTGA DGKYEFRTLM PVGYGCPPQG ATQQLLDRLG RHGNRPAHVH FFVTSDGHRK LTTQFNIEGD PLIWDDFAYA TREELIPPVT AKAGGAALGL KADAYQDIEF NFVLTPRVEG KDNQIVERLR ASATA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg tcaaagtttt cgatacgaag gaagtgcagg atctgctgaa ggccgcgtcg aacgcgggca cgaacggcgc gaacggcacg caggccggca acgcgcgcac gcagcagatc gtccaccggc tgctcggcga cctgttcaag gcgatcgacg atctcgacat cacgcccgac gaagtgtggg ccggcgtgaa ctacctgaac aagctcggcc aggacggcga agcggcgctg ctcgcggcgg gcctcggcct cgagaagtat ctcgacatcc ggatggacgc cgaggacgag gcgatcggcc tggacggcgg cacgccgcgc acgatcgaag gcccgctgta tgtcgccggc gcgccggtgc gcgacggcgt cgcgaagatc gatctcgacg cggacgaagg cgcgggcccg ctcgtgatcc acggcacggt cacggggctc gacggcaagc cggtggccgg cgcgctggtc gagtgctggc acgcgaactc gcacggcttc tattcgcact tcgacccgac cggcaagcag agcgatttca acctgcgcgg cgcggtgaag acgggcgcgg acggcaagta cgaattccgc acgctgatgc cggtcggcta cggctgcccg ccgcagggcg cgacgcagca gctgctggat cgcctcggcc gtcacggcaa ccgtccggcg cacgtgcact tcttcgtgac cagcgacggc caccgcaagc tgacgacgca gttcaacatc gaaggcgacc cgctgatctg ggatgacttc gcgtatgcga cgcgcgagga gctgattccg cccgtcactg ccaaggccgg cggcgccgcg ctcgggctga aggccgacgc gtatcaggac atcgagttca acttcgtcct gacgccgcgc gtcgagggca aggacaacca gatcgtcgaa cgcctgcgcg cttccgccac ggcatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat