SSGCID: Target Page

BuamA.00117.a

Intradiol ring-cleavage dioxygenase

CENTER ID:	BuamA.00117.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00117.a.B1.GE39783	Full length( BuamA.00117.a )		1	290

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00117.a.B1.PS37923	Full length( BuamA.00117.a )		1	290

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.3481
RefSeq:	YP_001810061.1
UniProt:	B1YZ80

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTNEHHDGDT RLTEQVVASF DGTPNPRLRV LMQSLVRHLH AFVRETELTE AEWMTAIRFL TETGQMCDDV VRQEFILLSD TLGVSMLVDA INHRFATGAT ETTVFGPFYI EGMPERAYGE NMAFTPGTPV VVHGRVLATD GAPVPGAVLD VWQTADNGMY SGQDNAQPHG NLRGRYRTDA EGRYAITTIL PVSYPIPTDG PVGKMLDATG RHPWRPAHLH FMIDAPGYRK LVTHLFDEED AYLESDAVFG VKPSLMVAYR PRAAGDELAR RFGLSGAYRE ATYDFVLDRS

NT Sequence

GTGACGAATG AACATCATGA CGGCGATACC CGGCTGACCG AGCAGGTCGT CGCCAGCTTC GACGGTACGC CGAACCCGCG CTTGCGCGTG CTGATGCAGA GCCTCGTGCG GCACCTGCAT GCGTTTGTCC GCGAAACGGA GCTGACCGAG GCCGAGTGGA TGACCGCGAT CCGCTTCCTG ACCGAAACCG GGCAGATGTG CGACGACGTC GTGCGCCAGG AATTCATCCT GTTGTCGGAC ACGCTGGGCG TGTCGATGCT GGTCGATGCG ATCAACCACC GTTTCGCCAC CGGCGCGACG GAAACGACCG TGTTCGGGCC GTTCTACATC GAGGGCATGC CCGAGCGTGC GTACGGCGAG AACATGGCGT TCACGCCCGG CACGCCCGTC GTCGTGCACG GCCGCGTGCT CGCCACCGAC GGTGCGCCGG TGCCGGGCGC CGTGCTCGAC GTGTGGCAGA CCGCCGACAA CGGCATGTAT TCCGGGCAGG ACAACGCACA GCCGCACGGC AATCTGCGCG GCCGCTACCG GACCGATGCC GAAGGCCGTT ATGCGATTAC GACGATCCTG CCGGTCAGCT ATCCGATTCC GACCGACGGC CCGGTCGGCA AGATGCTCGA TGCCACCGGA CGCCATCCGT GGCGGCCCGC GCACCTGCAC TTCATGATCG ATGCGCCGGG CTACCGCAAG CTCGTCACGC ATCTGTTCGA CGAGGAGGAC GCGTATCTGG AGTCGGATGC CGTGTTCGGC GTGAAGCCGT CGCTGATGGT CGCCTATCGG CCGCGTGCGG CGGGCGATGA ACTTGCACGC CGGTTCGGGT TGAGCGGCGC GTACCGCGAA GCGACGTATG ACTTCGTGCT CGACCGGAGC

Details for BuamA.00117.a.B1.GE39783

HARVESTED ON:	2/4/2016
SEQUENCED ON:	1/27/2016
EXPECTED MW:	33kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV TNEHHDGDTR LTEQVVASFD GTPNPRLRVL MQSLVRHLHA FVRETELTEA EWMTAIRFLT ETGQMCDDVV RQEFILLSDT LGVSMLVDAI NHRFATGATE TTVFGPFYIE GMPERAYGEN MAFTPGTPVV VHGRVLATDG APVPGAVLDV WQTADNGMYS GQDNAQPHGN LRGRYRTDAE GRYAITTILP VSYPIPTDGP VGKMLDATGR HPWRPAHLHF MIDAPGYRKL VTHLFDEEDA YLESDAVFGV KPSLMVAYRP RAAGDELARR FGLSGAYREA TXXFVLDRS

Validated NT Sequence

atcttactca cttagctccg gtcgagcacg aannnntacg tcgcttcgcg gtacgcgccg ctcaacccga accggcgtgc aagttcatcg cccgccgcac gcggccgata ggcgaccatc agcgacggct tcacgccgaa cacggcatcc gactccagat acgcgtcctc ctcgtcgaac agatgcgtga cgagcttgcg gtagcccggc gcatcgatca tgaagtgcag gtgcgcgggc cgccacggat ggcgtccggt ggcatcgagc atcttgccga ccgggccgtc ggtcggaatc ggatagctga ccggcaggat cgtcgtaatc gcataacggc cttcggcatc ggtccggtag cggccgcgca gattgccgtg cggctgtgcg ttgtcctgcc cggaatacat gccgttgtcg gcggtctgcc acacgtcgag cacggcgccc ggcaccggcg caccgtcggt ggcgagcacg cggccgtgca cgacgacggg cgtgccgggc gtgaacgcca tgttctcgcc gtacgcacgc tcgggcatgc cctcgatgta gaacggcccg aacacggtcg tttccgtcgc gccggtggcg aaacggtggt tgatcgcatc gaccagcatc gacacgccca gcgtgtccga caacaggatg aattcctggc gcacgacgtc gtcgcacatc tgcccggttt cggtcaggaa gcggatcgcg gtcatccact cggcctcggt cagctccgtt tcgcggacaa acgcatgcag gtgccgcacg aggctctgca tcagcacgcg caagcgcggg ttcggcgtac cgtcgaagct ggcgacgacc tgctcggtca gccgggtatc gccgtcatga tgttcattcg tcaccatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

MAHHHHHHMT NEHHDGDTRL TEQVVASFDG TPNPRLRVLM QSLVRHLHAF VRETELTEAE WMTAIRFLTE TGQMCDDVVR QEFILLSDTL GVSMLVDAIN HRFATGATET TVFGPFYIEG MPERAYGENM AFTPGTPVVV HGRVLATDGA PVPGAVLDVW QTADNGMYSG QDNAQPHGNL RGRYRTDAEG RYAITTILPV SYPIPTDGPV GKMLDATGRH PWRPAHLHFM IDAPGYRKLV THLFDEEDAY LESDAVFGVK PSLMVAYRPR AAGDELARRF GLSGAYREAT YDFVLDRS

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacga atgaacatca tgacggcgat acccggctga ccgagcaggt cgtcgccagc ttcgacggta cgccgaaccc gcgcttgcgc gtgctgatgc agagcctcgt gcggcacctg catgcgtttg tccgcgaaac ggagctgacc gaggccgagt ggatgaccgc gatccgcttc ctgaccgaaa ccgggcagat gtgcgacgac gtcgtgcgcc aggaattcat cctgttgtcg gacacgctgg gcgtgtcgat gctggtcgat gcgatcaacc accgtttcgc caccggcgcg acggaaacga ccgtgttcgg gccgttctac atcgagggca tgcccgagcg tgcgtacggc gagaacatgg cgttcacgcc cggcacgccc gtcgtcgtgc acggccgcgt gctcgccacc gacggtgcgc cggtgccggg cgccgtgctc gacgtgtggc agaccgccga caacggcatg tattccgggc aggacaacgc acagccgcac ggcaatctgc gcggccgcta ccggaccgat gccgaaggcc gttatgcgat tacgacgatc ctgccggtca gctatccgat tccgaccgac ggcccggtcg gcaagatgct cgatgccacc ggacgccatc cgtggcggcc cgcgcacctg cacttcatga tcgatgcgcc gggctaccgc aagctcgtca cgcatctgtt cgacgaggag gacgcgtatc tggagtcgga tgccgtgttc ggcgtgaagc cgtcgctgat ggtcgcctat cggccgcgtg cggcgggcga tgaacttgca cgccggttcg ggttgagcgg cgcgtaccgc gaagcgacgt atgacttcgt gctcgaccgg agctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for BuamA.00117.a.B1.PS37923

PURIFICATION DATe:	4/27/2016
CONCENTRATION:	39.9mg/ml
OBSERVED MW:	33kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	8
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACGA ATGAACATCA TGACGGCGAT ACCCGGCTGA CCGAGCAGGT CGTCGCCAGC TTCGACGGTA CGCCGAACCC GCGCTTGCGC GTGCTGATGC AGAGCCTCGT GCGGCACCTG CATGCGTTTG TCCGCGAAAC GGAGCTGACC GAGGCCGAGT GGATGACCGC GATCCGCTTC CTGACCGAAA CCGGGCAGAT GTGCGACGAC GTCGTGCGCC AGGAATTCAT CCTGTTGTCG GACACGCTGG GCGTGTCGAT GCTGGTCGAT GCGATCAACC ACCGTTTCGC CACCGGCGCG ACGGAAACGA CCGTGTTCGG GCCGTTCTAC ATCGAGGGCA TGCCCGAGCG TGCGTACGGC GAGAACATGG CGTTCACGCC CGGCACGCCC GTCGTCGTGC ACGGCCGCGT GCTCGCCACC GACGGTGCGC CGGTGCCGGG CGCCGTGCTC GACGTGTGGC AGACCGCCGA CAACGGCATG TATTCCGGGC AGGACAACGC ACAGCCGCAC GGCAATCTGC GCGGCCGCTA CCGGACCGAT GCCGAAGGCC GTTATGCGAT TACGACGATC CTGCCGGTCA GCTATCCGAT TCCGACCGAC GGCCCGGTCG GCAAGATGCT CGATGCCACC GGACGCCATC CGTGGCGGCC CGCGCACCTG CACTTCATGA TCGATGCGCC GGGCTACCGC AAGCTCGTCA CGCATCTGTT CGACGAGGAG GACGCGTATC TGGAGTCGGA TGCCGTGTTC GGCGTGAAGC CGTCGCTGAT GGTCGCCTAT CGGCCGCGTG CGGCGGGCGA TGAACTTGCA CGCCGGTTCG GGTTGAGCGG CGCGTACCGC GAAGCGACGT ATGACTTCGT GCTCGACCGG AGCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT