BuamA.00116.c

4-hydroxythreonine-4-phosphate dehydrogenase

CENTER ID: BuamA.00116.c
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00116.c.A1.GE33134 Full length( BuamA.00116.c ) 1 339
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00116.c.A1.PS01245 Full length( BuamA.00116.c ) 1 339

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.6427
RefSeq: YP_001815952.1
UniProt: B1Z3T6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDNRPIIGIT MGDAAGVGPE IIMKSLAHAS VYEQCRPVVI GDAARLRDAG RLAGVDLDVR AVSRPADGAF RVGVVDCIDM ALIPADLPYG KLSAVAGDAA YQYIARTVEL TSKGELDAIC TAPLNKEALH AGGHIFPGHT EMLAHLTGID EVSMMLVAPN LRVIHVTTHI GLLDAIRKIE PGLVQRTIER AHDTLVRAGI ANPRIGVCGI NPHAGENGLF GYGEEEEKIV PAVQILQARG WDVEGPLPAD TLFFRAGRGD FDVVVAMYHD QGHGPVKVMG LEAGVNVTVG LPVIRTSVDH GTAFDIAGKG IADERSLLEA FRQAVELATR RNEPAPVAA
NT Sequence
ATGGACAACC GCCCCATCAT CGGCATCACC ATGGGCGACG CGGCCGGCGT CGGACCGGAA ATCATCATGA AGAGCCTTGC CCATGCGTCC GTGTATGAGC AGTGCCGTCC CGTGGTCATC GGCGACGCCG CGCGCCTGCG CGACGCCGGC CGCCTGGCGG GCGTCGATCT CGACGTGCGT GCCGTCAGCC GGCCGGCCGA CGGCGCGTTC CGCGTGGGCG TCGTCGATTG CATCGACATG GCGCTCATTC CGGCCGACCT GCCCTACGGC AAGCTGTCCG CCGTCGCCGG AGACGCCGCG TATCAATACA TCGCGCGCAC CGTCGAGCTG ACGTCGAAAG GCGAGCTCGA CGCGATCTGC ACCGCGCCGC TCAACAAGGA AGCGCTGCAT GCCGGCGGCC ATATTTTCCC CGGACACACG GAAATGCTCG CGCATCTGAC GGGCATCGAC GAAGTGTCGA TGATGCTGGT CGCGCCCAAC CTTCGCGTGA TCCACGTGAC GACGCACATC GGCCTGCTCG ACGCGATTCG CAAGATCGAG CCGGGCCTCG TGCAACGCAC GATCGAGCGC GCACACGACA CGCTGGTTCG CGCCGGCATC GCGAATCCCC GCATCGGCGT ATGCGGGATC AATCCCCATG CCGGCGAGAA CGGCCTGTTC GGCTACGGCG AAGAGGAAGA GAAGATCGTT CCGGCGGTCC AGATCCTCCA GGCGCGCGGA TGGGACGTGG AAGGCCCGCT GCCGGCGGAC ACGCTGTTCT TCCGTGCGGG CCGCGGCGAT TTCGACGTGG TCGTCGCGAT GTATCACGAC CAGGGGCACG GGCCGGTGAA GGTGATGGGC CTCGAAGCCG GCGTGAACGT GACGGTCGGC CTGCCGGTCA TCCGCACGTC GGTGGACCAC GGCACCGCAT TCGACATCGC CGGCAAGGGC ATCGCCGACG AACGCAGCCT GCTGGAGGCT TTCCGCCAGG CCGTGGAACT TGCCACGCGA CGCAACGAGC CGGCGCCAGT CGCCGCG
Details for BuamA.00116.c.A1.GE33134
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MDNRPIIGIT MGDAAGVGPE IIMKSLAHAS VYEQCRPVVI GDAARLRDAG RLAGVDLDVR AVSRPADGAF RVGVVDCIDM ALIPADLPYG KLSAVAGDAA YQYIARTVEL TSKGELDAIC TAPLNKEALH AGGHIFPGHT EMLAHLTGID EVSMMLVAPN LRVIHVTTHI GLLDAIRKIE PGLVQRTIER AHDTLVRAGI ANPRIGVCGI NPHAGENGLF GYGEEEEKIV PAVQILQARG WDVEGPLPAD TLFFRAGRGD FDVVVAMYHD QGHGPVKVMG LEAGVNVTVG LPVIRTSVDH GTAFDIAGKG IADERSLLEA FRQAVELATR RNEPAPVAA
Validated NT Sequence
ttattacgcg gcgactggcg ccggctcgtt gcgtcgcgtg gcaagttcca cggcctggcg gaaagcctcc agcaggctgc gttcgtcggc gatgcccttg ccggcgatgt cgaatgcggt gccgtggtcc accgacgtgc ggatgaccgg caggccgacc gtcacgttca cgccggcttc gaggcccatc accttcaccg gcccgtgccc ctggtcgtga tacatcgcga cgaccacgtc gaaatcgccg cggcccgcac ggaagaacag cgtgtccgcc ggcagcgggc cttccacgtc ccatccgcgc gcctggagga tctggaccgc cggaacgatc ttctcttcct cttcgccgta gccgaacagg ccgttctcgc cggcatgggg attgatcccg catacgccga tgcggggatt cgcgatgccg gcgcgaacca gcgtgtcgtg tgcgcgctcg atcgtgcgtt gcacgaggcc cggctcgatc ttgcgaatcg cgtcgagcag gccgatgtgc gtcgtcacgt ggatcacgcg aaggttgggc gcgaccagca tcatcgacac ttcgtcgatg cccgtcagat gcgcgagcat ttccgtgtgt ccggggaaaa tatggccgcc ggcatgcagc gcttccttgt tgagcggcgc ggtgcagatc gcgtcgagct cgcctttcga cgtcagctcg acggtgcgcg cgatgtattg atacgcggcg tctccggcga cggcggacag cttgccgtag ggcaggtcgg ccggaatgag cgccatgtcg atgcaatcga cgacgcccac gcggaacgcg ccgtcggccg gccggctgac ggcacgcacg tcgagatcga cgcccgccag gcggccggcg tcgcgcaggc gcgcggcgtc gccgatgacc acgggacggc actgctcata cacggacgca tgggcaaggc tcttcatgat gatttccggt ccgacgccgg ccgcgtcgcc catggtgatg ccgatgatgg ggcggttgtc catcgaacca ggaccctggg tctgagcttc cagggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDNRPIIGI TMGDAAGVGP EIIMKSLAHA SVYEQCRPVV IGDAARLRDA GRLAGVDLDV RAVSRPADGA FRVGVVDCID MALIPADLPY GKLSAVAGDA AYQYIARTVE LTSKGELDAI CTAPLNKEAL HAGGHIFPGH TEMLAHLTGI DEVSMMLVAP NLRVIHVTTH IGLLDAIRKI EPGLVQRTIE RAHDTLVRAG IANPRIGVCG INPHAGENGL FGYGEEEEKI VPAVQILQAR GWDVEGPLPA DTLFFRAGRG DFDVVVAMYH DQGHGPVKVM GLEAGVNVTV GLPVIRTSVD HGTAFDIAGK GIADERSLLE AFRQAVELAT RRNEPAPVAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaaccgc cccatcatcg gcatcaccat gggcgacgcg gccggcgtcg gaccggaaat catcatgaag agccttgccc atgcgtccgt gtatgagcag tgccgtcccg tggtcatcgg cgacgccgcg cgcctgcgcg acgccggccg cctggcgggc gtcgatctcg acgtgcgtgc cgtcagccgg ccggccgacg gcgcgttccg cgtgggcgtc gtcgattgca tcgacatggc gctcattccg gccgacctgc cctacggcaa gctgtccgcc gtcgccggag acgccgcgta tcaatacatc gcgcgcaccg tcgagctgac gtcgaaaggc gagctcgacg cgatctgcac cgcgccgctc aacaaggaag cgctgcatgc cggcggccat attttccccg gacacacgga aatgctcgcg catctgacgg gcatcgacga agtgtcgatg atgctggtcg cgcccaacct tcgcgtgatc cacgtgacga cgcacatcgg cctgctcgac gcgattcgca agatcgagcc gggcctcgtg caacgcacga tcgagcgcgc acacgacacg ctggttcgcg ccggcatcgc gaatccccgc atcggcgtat gcgggatcaa tccccatgcc ggcgagaacg gcctgttcgg ctacggcgaa gaggaagaga agatcgttcc ggcggtccag atcctccagg cgcgcggatg ggacgtggaa ggcccgctgc cggcggacac gctgttcttc cgtgcgggcc gcggcgattt cgacgtggtc gtcgcgatgt atcacgacca ggggcacggg ccggtgaagg tgatgggcct cgaagccggc gtgaacgtga cggtcggcct gccggtcatc cgcacgtcgg tggaccacgg caccgcattc gacatcgccg gcaagggcat cgccgacgaa cgcagcctgc tggaggcttt ccgccaggcc gtggaacttg ccacgcgacg caacgagccg gcgccagtcg ccgcgtaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuamA.00116.c.A1.PS01245
PURIFICATION DATe: 10/18/2011
CONCENTRATION: 55.89mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MDNRPIIGIT MGDAAGVGPE IIMKSLAHAS VYEQCRPVVI GDAARLRDAG RLAGVDLDVR AVSRPADGAF RVGVVDCIDM ALIPADLPYG KLSAVAGDAA YQYIARTVEL TSKGELDAIC TAPLNKEALH AGGHIFPGHT EMLAHLTGID EVSMMLVAPN LRVIHVTTHI GLLDAIRKIE PGLVQRTIER AHDTLVRAGI ANPRIGVCGI NPHAGENGLF GYGEEEEKIV PAVQILQARG WDVEGPLPAD TLFFRAGRGD FDVVVAMYHD QGHGPVKVMG LEAGVNVTVG LPVIRTSVDH GTAFDIAGKG IADERSLLEA FRQAVELATR RNEPAPVAA
Validated NT Sequence
ttattacgcg gcgactggcg ccggctcgtt gcgtcgcgtg gcaagttcca cggcctggcg gaaagcctcc agcaggctgc gttcgtcggc gatgcccttg ccggcgatgt cgaatgcggt gccgtggtcc accgacgtgc ggatgaccgg caggccgacc gtcacgttca cgccggcttc gaggcccatc accttcaccg gcccgtgccc ctggtcgtga tacatcgcga cgaccacgtc gaaatcgccg cggcccgcac ggaagaacag cgtgtccgcc ggcagcgggc cttccacgtc ccatccgcgc gcctggagga tctggaccgc cggaacgatc ttctcttcct cttcgccgta gccgaacagg ccgttctcgc cggcatgggg attgatcccg catacgccga tgcggggatt cgcgatgccg gcgcgaacca gcgtgtcgtg tgcgcgctcg atcgtgcgtt gcacgaggcc cggctcgatc ttgcgaatcg cgtcgagcag gccgatgtgc gtcgtcacgt ggatcacgcg aaggttgggc gcgaccagca tcatcgacac ttcgtcgatg cccgtcagat gcgcgagcat ttccgtgtgt ccggggaaaa tatggccgcc ggcatgcagc gcttccttgt tgagcggcgc ggtgcagatc gcgtcgagct cgcctttcga cgtcagctcg acggtgcgcg cgatgtattg atacgcggcg tctccggcga cggcggacag cttgccgtag ggcaggtcgg ccggaatgag cgccatgtcg atgcaatcga cgacgcccac gcggaacgcg ccgtcggccg gccggctgac ggcacgcacg tcgagatcga cgcccgccag gcggccggcg tcgcgcaggc gcgcggcgtc gccgatgacc acgggacggc actgctcata cacggacgca tgggcaaggc tcttcatgat gatttccggt ccgacgccgg ccgcgtcgcc catggtgatg ccgatgatgg ggcggttgtc catcgaacca ggaccctggg tctgagcttc cagggta
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDNRPIIGI TMGDAAGVGP EIIMKSLAHA SVYEQCRPVV IGDAARLRDA GRLAGVDLDV RAVSRPADGA FRVGVVDCID MALIPADLPY GKLSAVAGDA AYQYIARTVE LTSKGELDAI CTAPLNKEAL HAGGHIFPGH TEMLAHLTGI DEVSMMLVAP NLRVIHVTTH IGLLDAIRKI EPGLVQRTIE RAHDTLVRAG IANPRIGVCG INPHAGENGL FGYGEEEEKI VPAVQILQAR GWDVEGPLPA DTLFFRAGRG DFDVVVAMYH DQGHGPVKVM GLEAGVNVTV GLPVIRTSVD HGTAFDIAGK GIADERSLLE AFRQAVELAT RRNEPAPVAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGACAACCGC CCCATCATCG GCATCACCAT GGGCGACGCG GCCGGCGTCG GACCGGAAAT CATCATGAAG AGCCTTGCCC ATGCGTCCGT GTATGAGCAG TGCCGTCCCG TGGTCATCGG CGACGCCGCG CGCCTGCGCG ACGCCGGCCG CCTGGCGGGC GTCGATCTCG ACGTGCGTGC CGTCAGCCGG CCGGCCGACG GCGCGTTCCG CGTGGGCGTC GTCGATTGCA TCGACATGGC GCTCATTCCG GCCGACCTGC CCTACGGCAA GCTGTCCGCC GTCGCCGGAG ACGCCGCGTA TCAATACATC GCGCGCACCG TCGAGCTGAC GTCGAAAGGC GAGCTCGACG CGATCTGCAC CGCGCCGCTC AACAAGGAAG CGCTGCATGC CGGCGGCCAT ATTTTCCCCG GACACACGGA AATGCTCGCG CATCTGACGG GCATCGACGA AGTGTCGATG ATGCTGGTCG CGCCCAACCT TCGCGTGATC CACGTGACGA CGCACATCGG CCTGCTCGAC GCGATTCGCA AGATCGAGCC GGGCCTCGTG CAACGCACGA TCGAGCGCGC ACACGACACG CTGGTTCGCG CCGGCATCGC GAATCCCCGC ATCGGCGTAT GCGGGATCAA TCCCCATGCC GGCGAGAACG GCCTGTTCGG CTACGGCGAA GAGGAAGAGA AGATCGTTCC GGCGGTCCAG ATCCTCCAGG CGCGCGGATG GGACGTGGAA GGCCCGCTGC CGGCGGACAC GCTGTTCTTC CGTGCGGGCC GCGGCGATTT CGACGTGGTC GTCGCGATGT ATCACGACCA GGGGCACGGG CCGGTGAAGG TGATGGGCCT CGAAGCCGGC GTGAACGTGA CGGTCGGCCT GCCGGTCATC CGCACGTCGG TGGACCACGG CACCGCATTC GACATCGCCG GCAAGGGCAT CGCCGACGAA CGCAGCCTGC TGGAGGCTTT CCGCCAGGCC GTGGAACTTG CCACGCGACG CAACGAGCCG GCGCCAGTCG CCGCGTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA