BuamA.00113.c

Beta-ketoacyl synthase

CENTER ID: BuamA.00113.c
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00113.c.A1.GE32133 Full length( BuamA.00113.c ) 1 412
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.4215
RefSeq: YP_001810768.1
UniProt: B1Z2X2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAVGGAATR VVITGLGIVS CLGNSLTEVS DALRTGRSGV SRVDAWRERG FRSQVAGVAS VASAARFARK HERFMGDTAR FAAHAAASAL DDAGLDAGML RSPRAGAIVG SGIGSIADYD DAMEIARHRG IEKTPPYVVP KAMSSTASAC VAQLFGIEGV SYSPSSACTS AALAIGQAAH WIRSGHQDIV LAGGSEALHD NMTLMFDAMG ALSSGFNDTP ERASRPYALG RDGFVIASGA GILVLESLEH ARARGARIYA EISGFGQATD NAGMAAPHAP AISRAMRGAL DGSGRHPDYV NTHAPSTPLG DTEELDALRD VFGGDVPAFS STKGLTGHPL GASGAHEAIY TILMMRDGFV AGTAVGFEPD PAVAGMPLVR TARECSIERA LSVSFGFGGS CASVLLDAWK GD
NT Sequence
ATGACGGCGG TCGGCGGCGC GGCGACGCGC GTCGTGATCA CGGGGCTCGG CATCGTGTCG TGCCTCGGCA ATTCGCTCAC CGAAGTATCT GACGCGCTGC GCACCGGCCG GTCGGGGGTG AGCCGCGTCG ACGCGTGGCG CGAGCGCGGT TTCCGCAGCC AGGTTGCGGG GGTGGCGAGC GTGGCGTCGG CGGCGCGCTT CGCGCGCAAG CACGAGCGCT TCATGGGCGA CACCGCGCGT TTCGCCGCGC ATGCGGCCGC GTCGGCACTC GACGATGCAG GGCTCGACGC AGGGATGCTG CGTTCGCCGC GCGCGGGCGC GATCGTGGGG TCGGGCATCG GCTCGATCGC CGACTACGAC GACGCGATGG AGATCGCGCG GCATCGCGGT ATCGAGAAGA CACCGCCTTA TGTCGTGCCG AAAGCGATGA GCAGCACGGC GTCGGCATGC GTCGCGCAAT TGTTCGGCAT CGAAGGCGTG TCGTACTCGC CGTCGTCCGC CTGCACGAGC GCGGCGCTGG CGATCGGACA GGCGGCGCAC TGGATTCGCT CCGGGCATCA GGACATCGTG CTGGCCGGTG GGAGCGAGGC ACTGCACGAC AACATGACGC TGATGTTCGA CGCGATGGGC GCGTTGTCGA GCGGCTTCAA CGATACGCCG GAGCGCGCAT CGCGCCCGTA TGCGCTCGGC CGCGACGGCT TCGTGATCGC GTCCGGCGCC GGCATTCTCG TGCTCGAATC GCTGGAGCAT GCGCGTGCGC GCGGCGCGCG AATTTACGCG GAAATCAGCG GGTTCGGCCA AGCCACGGAC AACGCCGGCA TGGCGGCGCC TCATGCACCG GCGATCTCAC GCGCGATGCG TGGCGCACTC GACGGCAGCG GGCGGCATCC GGACTACGTC AACACACATG CGCCGTCCAC GCCGCTCGGC GACACCGAGG AGCTCGACGC GCTGCGCGAC GTATTCGGTG GCGACGTGCC GGCCTTCTCG TCAACGAAAG GACTGACAGG ACATCCGCTC GGCGCGAGCG GCGCGCATGA AGCGATCTAC ACGATCCTGA TGATGCGCGA CGGTTTTGTC GCGGGCACCG CGGTCGGCTT CGAGCCCGAT CCGGCGGTCG CCGGCATGCC GCTCGTGCGT ACCGCGCGCG AATGCTCGAT CGAACGCGCG CTGTCGGTGT CGTTCGGATT CGGCGGCAGC TGCGCAAGTG TGTTGTTGGA CGCCTGGAAG GGCGAT
Details for BuamA.00113.c.A1.GE32133
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 44kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 77
Validated AA Sequence
MGDTARFAAH AAASALDDAG LDAGMLRSPR AGAIVGSGIG SIADYDDAME IARHRGIEKT PPYVVPKAMS STASACVAQL FGIEGVSYSP SSACTSAALA IGQAAHWIRS GXQDIVLAGG SEALHDNMTL MFDAMGALSS GFNDTPERAS RPYALGRDGF VIASGAGILV LESLEHARAR GARIYAEISG FGQATDNAGM AAPHAPAISR AMRGALDGSG RHPDYVNTHA PSTPLGDTEE LDALRDVFGG DVPAFSSTKG LTGHPLGASG AHEAIYTILM MRDGFVAGTA VGFEPDPAVA GMPLVRTARE CSIERALSVS FGFGGSCASV LLDAWKG
Validated NT Sequence
cgcccttcca ggcgtccaac aacacacttg cgcagctgcc gccgaatccg aacgacaccg acagcgcgcg ttcgatcgag cattcgcgcg cggtacgcac gagcggcatg ccggcgaccg ccggatcggg ctcgaagccg accgcggtgc ccgcgacaaa accgtcgcgc atcatcagga tcgtgtagat cgcttcatgc gcgccgctcg cgccgagcgg atgtcctgtc agtcctttcg ttgacgagaa ggccggcacg tcgccaccga atacgtcgcg cagcgcgtcg agctcctcgg tgtcgccgag cggcgtggac ggcgcatgtg tgttgacgta gtccggatgc cgcccgctgc cgtcgagtgc gccacgcatc gcgcgtgaga tcgccggtgc atgaggcgcc gccatgccgg cgttgtccgt ggcttggccg aacccgctga tttccgcgta aattcgcgcg ccgcgcgcac gcgcatgctc cagcgattcg agcacgagaa tgccggcgcc ggacgcgatc acgaagccgt cgcggccgag cgcatacggg cgcgatgcgc gctccggcgt atcgttgaag ccgctcgaca acgcgcccat cgcgtcgaac atcagcgtca tgttgtcgtg cagtgcctcg ctcccaccgg ccagcacgat gtcctgntgc ccggagcgaa tccagtgcgc cgcctgtccg atcgccagcg ccgcgctcgt gcaggcggac gacggcgagt acgacacgcc ttcgatgccg aacaattgcg cgacgcatgc cgacgccgtg ctgctcatcg ctttcggcac gacataaggc ggtgtcttct cgataccgcg atgccgcgcg atctccatcg cgtcgtcgta gtcggcgatc gagccgatgc ccgaccccac gatcgcgccc gcgcgcggcg aacgcagcat ccctgcgtcg agccctgcat cgtcgagtgc cgacgcggcc gcatgcgcgg cgaaacgcgc ggtgtcgccc atgaagcgct cgtgcttgcg cgcgaagcgc gccgccgacg ccacgctcgc cacccccgca acctggctgc ggaaaccgcg ctcgcgccan gnntngncgc g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAVGGAAT RVVITGLGIV SCLGNSLTEV SDALRTGRSG VSRVDAWRER GFRSQVAGVA SVASAARFAR KHERFMGDTA RFAAHAAASA LDDAGLDAGM LRSPRAGAIV GSGIGSIADY DDAMEIARHR GIEKTPPYVV PKAMSSTASA CVAQLFGIEG VSYSPSSACT SAALAIGQAA HWIRSGHQDI VLAGGSEALH DNMTLMFDAM GALSSGFNDT PERASRPYAL GRDGFVIASG AGILVLESLE HARARGARIY AEISGFGQAT DNAGMAAPHA PAISRAMRGA LDGSGRHPDY VNTHAPSTPL GDTEELDALR DVFGGDVPAF SSTKGLTGHP LGASGAHEAI YTILMMRDGF VAGTAVGFEP DPAVAGMPLV RTARECSIER ALSVSFGFGG SCASVLLDAW KGD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggcggtc ggcggcgcgg cgacgcgcgt cgtgatcacg gggctcggca tcgtgtcgtg cctcggcaat tcgctcaccg aagtatctga cgcgctgcgc accggccggt cgggggtgag ccgcgtcgac gcgtggcgcg agcgcggttt ccgcagccag gttgcggggg tggcgagcgt ggcgtcggcg gcgcgcttcg cgcgcaagca cgagcgcttc atgggcgaca ccgcgcgttt cgccgcgcat gcggccgcgt cggcactcga cgatgcaggg ctcgacgcag ggatgctgcg ttcgccgcgc gcgggcgcga tcgtggggtc gggcatcggc tcgatcgccg actacgacga cgcgatggag atcgcgcggc atcgcggtat cgagaagaca ccgccttatg tcgtgccgaa agcgatgagc agcacggcgt cggcatgcgt cgcgcaattg ttcggcatcg aaggcgtgtc gtactcgccg tcgtccgcct gcacgagcgc ggcgctggcg atcggacagg cggcgcactg gattcgctcc gggcatcagg acatcgtgct ggccggtggg agcgaggcac tgcacgacaa catgacgctg atgttcgacg cgatgggcgc gttgtcgagc ggcttcaacg atacgccgga gcgcgcatcg cgcccgtatg cgctcggccg cgacggcttc gtgatcgcgt ccggcgccgg cattctcgtg ctcgaatcgc tggagcatgc gcgtgcgcgc ggcgcgcgaa tttacgcgga aatcagcggg ttcggccaag ccacggacaa cgccggcatg gcggcgcctc atgcaccggc gatctcacgc gcgatgcgtg gcgcactcga cggcagcggg cggcatccgg actacgtcaa cacacatgcg ccgtccacgc cgctcggcga caccgaggag ctcgacgcgc tgcgcgacgt attcggtggc gacgtgccgg ccttctcgtc aacgaaagga ctgacaggac atccgctcgg cgcgagcggc gcgcatgaag cgatctacac gatcctgatg atgcgcgacg gttttgtcgc gggcaccgcg gtcggcttcg agcccgatcc ggcggtcgcc ggcatgccgc tcgtgcgtac cgcgcgcgaa tgctcgatcg aacgcgcgct gtcggtgtcg ttcggattcg gcggcagctg cgcaagtgtg ttgttggacg cctggaaggg cgattaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa