BuamA.00109.b

N-isopropylammelide isopropylaminohydrolase

CENTER ID: BuamA.00109.b
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00109.b.B1.GE39780 Full length( BuamA.00109.b ) 1 413
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00109.b.B1.PS37914 Full length( BuamA.00109.b ) 1 413

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.4360
RefSeq: YP_001810899.1
UniProt: B1Z3A3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNLFNARLRG RDGLFTIGID GDRIARIDAQ TAPVAPAGAD DIDAGGRVAI PPLVEPHIHL DAVLTAGEPA WNMSGTLFEG IERWSERKAT ITHEDTKTRA HAAIGMLRDH GIQHVRTHVD VTDPTLAALK AMLEVKDEAR GLIDLQIVAF PQEGIESFDG GRALMEQAID LGADVVGGIP HFENTREQGV SSIRFLMELA ERTGCLVDVH CDETDDPHSR FLEVLAEEAR VRGMGARVTA SHTTAMGSYD NAYCSKLFRL LKRAGLNFVS CPTESIHLQG RFDMFPKRRG VTRVAELDRA GLNVCFGQDS IKDPWYPLGN GNILRVLDAG LHICHMMGYE DLQRCLDFVT DHSATTMHLG DGYGIAVGRP ANLVVLDADS DYEAVRRQAK ATLSVRHGKV IMRREPERVT YPE
NT Sequence
ATGAATCTCT TCAATGCACG CCTGCGCGGT CGCGACGGGC TCTTCACGAT CGGTATCGAC GGCGACAGGA TCGCGCGGAT CGACGCGCAA ACCGCGCCGG TCGCGCCCGC CGGCGCCGAC GATATCGATG CCGGCGGCCG TGTCGCGATC CCGCCGCTCG TCGAGCCGCA TATCCATCTC GATGCGGTGC TCACGGCCGG CGAGCCGGCC TGGAACATGA GCGGCACGCT GTTCGAGGGA ATCGAGCGCT GGTCCGAGCG CAAGGCGACG ATCACGCACG AGGACACCAA GACGCGCGCG CATGCGGCGA TCGGCATGCT GCGCGATCAC GGCATCCAGC ACGTGCGCAC CCACGTCGAC GTCACCGATC CGACGCTCGC CGCGCTGAAG GCGATGCTCG AAGTGAAGGA CGAGGCGCGC GGGCTGATCG ACCTGCAGAT CGTCGCGTTT CCGCAGGAAG GCATCGAGTC GTTCGACGGC GGCCGCGCGC TGATGGAGCA GGCGATCGAT CTCGGCGCGG ACGTCGTCGG CGGGATTCCG CATTTCGAGA ACACGCGCGA GCAGGGCGTC AGCTCGATCC GCTTCCTGAT GGAACTGGCC GAGCGTACCG GCTGCCTCGT CGACGTGCAT TGCGACGAGA CCGACGATCC GCATTCGCGC TTTCTCGAAG TGCTCGCGGA AGAAGCGCGC GTGCGCGGGA TGGGCGCGCG CGTGACGGCG AGCCACACCA CCGCGATGGG CTCGTACGAC AACGCGTACT GCTCGAAGCT GTTCCGGCTG CTCAAGCGTG CCGGGCTGAA CTTCGTGTCG TGTCCGACCG AGAGCATCCA CCTGCAGGGG CGTTTCGACA TGTTTCCGAA GCGGCGCGGC GTCACGCGCG TCGCCGAGCT CGATCGCGCG GGCCTGAACG TGTGCTTCGG GCAGGATTCG ATCAAGGATC CGTGGTATCC GCTCGGCAAC GGCAATATCC TGCGCGTGCT CGACGCCGGC CTCCATATCT GCCACATGAT GGGCTATGAG GACCTGCAGC GCTGCCTCGA CTTCGTGACC GATCACAGCG CGACGACGAT GCATCTCGGC GACGGTTACG GGATCGCGGT CGGGCGGCCG GCGAACCTGG TCGTGCTCGA CGCGGACAGC GACTACGAAG CGGTGCGCAG GCAGGCGAAG GCCACGCTGT CGGTTCGCCA CGGGAAGGTG ATCATGCGGC GGGAACCGGA GCGCGTCACG TATCCCGAG
Details for BuamA.00109.b.B1.GE39780
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMN LFNARLRGRD GLFTIGIDGD RIARIDAQTA PVAPAGADDI DAGGRVAIPP LVEPHIHLDA VLTAGEPAWN MSGTLFEGIE RWSERKATIT HEDTKTRAHA AIGMLRDHGI QHVRTHVDVT DPTLAALKAM LEVKDEARGL IDLQIVAFPQ EGIESFDGGR ALMEQAIDLG ADVVGGIPHF ENTREQGVSS IRFLMELAER TGCLVDVHCD ETDDPHSRFL EVLAEEARVR GMGARVTASH TTAMGSYDNA YCSKLFRLLK RAGLNFVSCP TESIHLQGRF DMFPKRRGVT RVAELDRAGL NVCFGQDSIK DPWYPLGNGN ILRVLDAGLH ICHMMGYEDL QRCLDFVTDH SATTMHLGDG YGIAVGRPAN LVVLDADSDY EAVRRQAKAT LSVRHGKVIM RR
Validated NT Sequence
cccgccgcat gatcaccttc ccgtggcgaa ccgacagcgt ggccttcgcc tgcctgcgca ccgcttcgta gtcgctgtcc gcgtcgagca cgaccaggtt cgccggccgc ccgaccgcga tcccgtaacc gtcgccgaga tgcatcgtcg tcgcgctgtg atcggtcacg aagtcgaggc agcgctgcag gtcctcatag cccatcatgt ggcagatatg gaggccggcg tcgagcacgc gcaggatatt gccgttgccg agcggatacc acggatcctt gatcgaatcc tgcccgaagc acacgttcag gcccgcgcga tcgagctcgg cgacgcgcgt gacgccgcgc cgcttcggaa acatgtcgaa acgcccctgc aggtggatgc tctcggtcgg acacgacacg aagttcagcc cggcacgctt gagcagccgg aacagcttcg agcagtacgc gttgtcgtac gagcccatcg cggtggtgtg gctcgccgtc acgcgcgcgc ccatcccgcg cacgcgcgct tcttccgcga gcacttcgag aaagcgcgaa tgcggatcgt cggtctcgtc gcaatgcacg tcgacgaggc agccggtacg ctcggccagt tccatcagga agcggatcga gctgacgccc tgctcgcgcg tgttctcgaa atgcggaatc ccgccgacga cgtccgcgcc gagatcgatc gcctgctcca tcagcgcgcg gccgccgtcg aacgactcga tgccttcctg cggaaacgcg acgatctgca ggtcgatcag cccgcgcgcc tcgtccttca cttcgagcat cgccttcagc gcggcgagcg tcggatcggt gacgtcgacg tgggtgcgca cgtgctggat gccgtgatcg cgcagcatgc cgatcgccgc atgcgcgcgc gtcttggtgt cctcgtgcgt gatcgtcgcc ttgcgctcgg accagcgctc gattccctcg aacagcgtgc cgctcatgtt ccaggccggc tcgccggccg tgagcaccgc atcgagatgg atatgcggct cgacgagcgg cgggatcgcg acacggccgc cggcatcgat atcgtcggcg ccggcgggcg cgaccggcgc ggtttgcgcg tcgatccgcg cgatcctgtc gccgtcgata ccgatcgtga agagcccgtc gcgaccgcgc aggcgtgcat tgaagagatt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMN LFNARLRGRD GLFTIGIDGD RIARIDAQTA PVAPAGADDI DAGGRVAIPP LVEPHIHLDA VLTAGEPAWN MSGTLFEGIE RWSERKATIT HEDTKTRAHA AIGMLRDHGI QHVRTHVDVT DPTLAALKAM LEVKDEARGL IDLQIVAFPQ EGIESFDGGR ALMEQAIDLG ADVVGGIPHF ENTREQGVSS IRFLMELAER TGCLVDVHCD ETDDPHSRFL EVLAEEARVR GMGARVTASH TTAMGSYDNA YCSKLFRLLK RAGLNFVSCP TESIHLQGRF DMFPKRRGVT RVAELDRAGL NVCFGQDSIK DPWYPLGNGN ILRVLDAGLH ICHMMGYEDL QRCLDFVTDH SATTMHLGDG YGIAVGRPAN LVVLDADSDY EAVRRQAKAT LSVRHGKVIM RREPERVTYP E
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatc tcttcaatgc acgcctgcgc ggtcgcgacg ggctcttcac gatcggtatc gacggcgaca ggatcgcgcg gatcgacgcg caaaccgcgc cggtcgcgcc cgccggcgcc gacgatatcg atgccggcgg ccgtgtcgcg atcccgccgc tcgtcgagcc gcatatccat ctcgatgcgg tgctcacggc cggcgagccg gcctggaaca tgagcggcac gctgttcgag ggaatcgagc gctggtccga gcgcaaggcg acgatcacgc acgaggacac caagacgcgc gcgcatgcgg cgatcggcat gctgcgcgat cacggcatcc agcacgtgcg cacccacgtc gacgtcaccg atccgacgct cgccgcgctg aaggcgatgc tcgaagtgaa ggacgaggcg cgcgggctga tcgacctgca gatcgtcgcg tttccgcagg aaggcatcga gtcgttcgac ggcggccgcg cgctgatgga gcaggcgatc gatctcggcg cggacgtcgt cggcgggatt ccgcatttcg agaacacgcg cgagcagggc gtcagctcga tccgcttcct gatggaactg gccgagcgta ccggctgcct cgtcgacgtg cattgcgacg agaccgacga tccgcattcg cgctttctcg aagtgctcgc ggaagaagcg cgcgtgcgcg ggatgggcgc gcgcgtgacg gcgagccaca ccaccgcgat gggctcgtac gacaacgcgt actgctcgaa gctgttccgg ctgctcaagc gtgccgggct gaacttcgtg tcgtgtccga ccgagagcat ccacctgcag gggcgtttcg acatgtttcc gaagcggcgc ggcgtcacgc gcgtcgccga gctcgatcgc gcgggcctga acgtgtgctt cgggcaggat tcgatcaagg atccgtggta tccgctcggc aacggcaata tcctgcgcgt gctcgacgcc ggcctccata tctgccacat gatgggctat gaggacctgc agcgctgcct cgacttcgtg accgatcaca gcgcgacgac gatgcatctc ggcgacggtt acgggatcgc ggtcgggcgg ccggcgaacc tggtcgtgct cgacgcggac agcgactacg aagcggtgcg caggcaggcg aaggccacgc tgtcggttcg ccacgggaag gtgatcatgc ggcgggaacc ggagcgcgtc acgtatcccg agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuamA.00109.b.B1.PS37914
PURIFICATION DATe: 4/21/2016
CONCENTRATION: 27.9mg/ml
OBSERVED MW: 45kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: SEC Buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN LFNARLRGRD GLFTIGIDGD RIARIDAQTA PVAPAGADDI DAGGRVAIPP LVEPHIHLDA VLTAGEPAWN MSGTLFEGIE RWSERKATIT HEDTKTRAHA AIGMLRDHGI QHVRTHVDVT DPTLAALKAM LEVKDEARGL IDLQIVAFPQ EGIESFDGGR ALMEQAIDLG ADVVGGIPHF ENTREQGVSS IRFLMELAER TGCLVDVHCD ETDDPHSRFL EVLAEEARVR GMGARVTASH TTAMGSYDNA YCSKLFRLLK RAGLNFVSCP TESIHLQGRF DMFPKRRGVT RVAELDRAGL NVCFGQDSIK DPWYPLGNGN ILRVLDAGLH ICHMMGYEDL QRCLDFVTDH SATTMHLGDG YGIAVGRPAN LVVLDADSDY EAVRRQAKAT LSVRHGKVIM RR
Validated NT Sequence
cccgccgcat gatcaccttc ccgtggcgaa ccgacagcgt ggccttcgcc tgcctgcgca ccgcttcgta gtcgctgtcc gcgtcgagca cgaccaggtt cgccggccgc ccgaccgcga tcccgtaacc gtcgccgaga tgcatcgtcg tcgcgctgtg atcggtcacg aagtcgaggc agcgctgcag gtcctcatag cccatcatgt ggcagatatg gaggccggcg tcgagcacgc gcaggatatt gccgttgccg agcggatacc acggatcctt gatcgaatcc tgcccgaagc acacgttcag gcccgcgcga tcgagctcgg cgacgcgcgt gacgccgcgc cgcttcggaa acatgtcgaa acgcccctgc aggtggatgc tctcggtcgg acacgacacg aagttcagcc cggcacgctt gagcagccgg aacagcttcg agcagtacgc gttgtcgtac gagcccatcg cggtggtgtg gctcgccgtc acgcgcgcgc ccatcccgcg cacgcgcgct tcttccgcga gcacttcgag aaagcgcgaa tgcggatcgt cggtctcgtc gcaatgcacg tcgacgaggc agccggtacg ctcggccagt tccatcagga agcggatcga gctgacgccc tgctcgcgcg tgttctcgaa atgcggaatc ccgccgacga cgtccgcgcc gagatcgatc gcctgctcca tcagcgcgcg gccgccgtcg aacgactcga tgccttcctg cggaaacgcg acgatctgca ggtcgatcag cccgcgcgcc tcgtccttca cttcgagcat cgccttcagc gcggcgagcg tcggatcggt gacgtcgacg tgggtgcgca cgtgctggat gccgtgatcg cgcagcatgc cgatcgccgc atgcgcgcgc gtcttggtgt cctcgtgcgt gatcgtcgcc ttgcgctcgg accagcgctc gattccctcg aacagcgtgc cgctcatgtt ccaggccggc tcgccggccg tgagcaccgc atcgagatgg atatgcggct cgacgagcgg cgggatcgcg acacggccgc cggcatcgat atcgtcggcg ccggcgggcg cgaccggcgc ggtttgcgcg tcgatccgcg cgatcctgtc gccgtcgata ccgatcgtga agagcccgtc gcgaccgcgc aggcgtgcat tgaagagatt catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHMN LFNARLRGRD GLFTIGIDGD RIARIDAQTA PVAPAGADDI DAGGRVAIPP LVEPHIHLDA VLTAGEPAWN MSGTLFEGIE RWSERKATIT HEDTKTRAHA AIGMLRDHGI QHVRTHVDVT DPTLAALKAM LEVKDEARGL IDLQIVAFPQ EGIESFDGGR ALMEQAIDLG ADVVGGIPHF ENTREQGVSS IRFLMELAER TGCLVDVHCD ETDDPHSRFL EVLAEEARVR GMGARVTASH TTAMGSYDNA YCSKLFRLLK RAGLNFVSCP TESIHLQGRF DMFPKRRGVT RVAELDRAGL NVCFGQDSIK DPWYPLGNGN ILRVLDAGLH ICHMMGYEDL QRCLDFVTDH SATTMHLGDG YGIAVGRPAN LVVLDADSDY EAVRRQAKAT LSVRHGKVIM RREPERVTYP E
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATC TCTTCAATGC ACGCCTGCGC GGTCGCGACG GGCTCTTCAC GATCGGTATC GACGGCGACA GGATCGCGCG GATCGACGCG CAAACCGCGC CGGTCGCGCC CGCCGGCGCC GACGATATCG ATGCCGGCGG CCGTGTCGCG ATCCCGCCGC TCGTCGAGCC GCATATCCAT CTCGATGCGG TGCTCACGGC CGGCGAGCCG GCCTGGAACA TGAGCGGCAC GCTGTTCGAG GGAATCGAGC GCTGGTCCGA GCGCAAGGCG ACGATCACGC ACGAGGACAC CAAGACGCGC GCGCATGCGG CGATCGGCAT GCTGCGCGAT CACGGCATCC AGCACGTGCG CACCCACGTC GACGTCACCG ATCCGACGCT CGCCGCGCTG AAGGCGATGC TCGAAGTGAA GGACGAGGCG CGCGGGCTGA TCGACCTGCA GATCGTCGCG TTTCCGCAGG AAGGCATCGA GTCGTTCGAC GGCGGCCGCG CGCTGATGGA GCAGGCGATC GATCTCGGCG CGGACGTCGT CGGCGGGATT CCGCATTTCG AGAACACGCG CGAGCAGGGC GTCAGCTCGA TCCGCTTCCT GATGGAACTG GCCGAGCGTA CCGGCTGCCT CGTCGACGTG CATTGCGACG AGACCGACGA TCCGCATTCG CGCTTTCTCG AAGTGCTCGC GGAAGAAGCG CGCGTGCGCG GGATGGGCGC GCGCGTGACG GCGAGCCACA CCACCGCGAT GGGCTCGTAC GACAACGCGT ACTGCTCGAA GCTGTTCCGG CTGCTCAAGC GTGCCGGGCT GAACTTCGTG TCGTGTCCGA CCGAGAGCAT CCACCTGCAG GGGCGTTTCG ACATGTTTCC GAAGCGGCGC GGCGTCACGC GCGTCGCCGA GCTCGATCGC GCGGGCCTGA ACGTGTGCTT CGGGCAGGAT TCGATCAAGG ATCCGTGGTA TCCGCTCGGC AACGGCAATA TCCTGCGCGT GCTCGACGCC GGCCTCCATA TCTGCCACAT GATGGGCTAT GAGGACCTGC AGCGCTGCCT CGACTTCGTG ACCGATCACA GCGCGACGAC GATGCATCTC GGCGACGGTT ACGGGATCGC GGTCGGGCGG CCGGCGAACC TGGTCGTGCT CGACGCGGAC AGCGACTACG AAGCGGTGCG CAGGCAGGCG AAGGCCACGC TGTCGGTTCG CCACGGGAAG GTGATCATGC GGCGGGAACC GGAGCGCGTC ACGTATCCCG AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT