BuamA.00109.a

N-isopropylammelide isopropylaminohydrolase

CENTER ID: BuamA.00109.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00109.a.B1.GE39779 Full length( BuamA.00109.a ) 1 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00109.a.B1.PS37918 Full length( BuamA.00109.a ) 1 394

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.5663
RefSeq: YP_001812110.1
UniProt: B1Z2D2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNLLIRNVR THADEALDIL IEGERIARVG PSLDAPAGCA IEDGAHALAL PGLVEGHTHL DKTHWGMPWY RNAVGPRLVD RIENERHYRA TSGHDAGAAS LALARAFLAA GTTRIRTHVD VDTDAGLRHL HGVLATRETL RGQVEIQIVA FPQSGVLKRP GTAALLSDAL GAGADLLGGL DPCAIEGDPV EAVDVLFGIA ERHGRGLDLH LHERGSMGAY SLDLILQRTA ALGMQGKVTI SHGFCLGDVA GRERDALLAR MAELGVAIVT TAPAAVPVPS VVACRAAGVT VVGGNDGVRD TWTPYGSPDM LERAMLIGMR NDFRRDDALE VALDCVTHSA ARGCGFADYG LEAGKRADVV LVDALTFAEA VVARPTRRLV VSSGRIVARD GVLV
NT Sequence
ATGACGAACC TGCTGATCCG CAACGTCCGC ACGCACGCCG ACGAAGCACT CGACATCCTG ATCGAAGGCG AGCGCATCGC GCGCGTCGGC CCGTCGCTCG ACGCGCCGGC GGGCTGCGCG ATCGAGGACG GCGCGCACGC GCTTGCGCTG CCCGGCCTCG TCGAAGGCCA CACGCATCTC GACAAGACCC ACTGGGGCAT GCCGTGGTAT CGCAACGCGG TGGGGCCGCG CCTCGTCGAC CGCATCGAGA ACGAGCGCCA CTACCGCGCG ACGAGCGGTC ACGATGCGGG CGCCGCGTCG CTCGCGCTGG CGCGCGCGTT CCTCGCGGCC GGCACGACGC GCATCCGCAC GCACGTCGAC GTCGACACCG ACGCCGGGCT GCGGCATCTG CATGGCGTGC TCGCAACGCG CGAGACGCTG CGCGGCCAGG TGGAGATCCA GATCGTCGCG TTTCCGCAGT CGGGCGTGCT CAAGCGGCCC GGCACCGCCG CGCTGCTGTC CGACGCGCTC GGCGCGGGCG CCGACCTGCT CGGCGGCCTC GACCCGTGCG CGATCGAAGG CGATCCGGTC GAAGCGGTGG ACGTGCTGTT CGGCATCGCC GAGCGCCACG GCCGCGGGCT CGACCTGCAC CTGCACGAGC GCGGGTCGAT GGGCGCGTAC TCGCTCGACC TGATCCTGCA GCGCACGGCC GCGCTCGGCA TGCAAGGCAA GGTCACGATC AGCCACGGCT TCTGCCTCGG CGACGTTGCC GGCCGCGAGC GCGACGCGCT GCTCGCGCGG ATGGCCGAAC TCGGCGTCGC GATCGTCACG ACCGCGCCGG CCGCGGTGCC CGTGCCGTCG GTCGTCGCGT GTCGGGCGGC CGGCGTGACC GTCGTCGGCG GCAACGACGG CGTGCGCGAT ACGTGGACGC CGTACGGTTC GCCCGACATG CTGGAACGCG CGATGCTGAT CGGCATGCGC AACGATTTCC GCCGCGACGA CGCGCTCGAA GTCGCACTCG ACTGCGTGAC GCACAGCGCG GCGCGTGGTT GCGGCTTCGC CGATTACGGG CTGGAGGCCG GCAAGCGTGC GGACGTCGTG CTCGTCGATG CGCTGACGTT CGCCGAGGCC GTCGTCGCGC GGCCGACGCG TCGGCTCGTC GTGTCGTCGG GGCGGATCGT CGCGCGCGAC GGCGTGCTGG TC
Details for BuamA.00109.a.B1.GE39779
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT NLLIRNVRTH ADEALDILIE GERIARVGPS LDAPAGCAIE DGAHALALPG LVEGHTHLDK THWGMPWYRN AVGPRLVDRI ENERHYRATS GHDAGAASLA LARAFLAAGT TRIRTHVDVD TDAGLRHLHG VLATRETLRG QVEIQIVAFP QSGVLKRPGT AALLSDALGA GADLLGGLDP CAIEGDPVEA VDVLFGIAER HGRGLDLHLH ERGSMGAYSL DLILQRTAAL GMQGKVTISH GFCLGDVAGR ERDALLARMA ELGVAIVTTA PAAVPVPSVV ACRAAGVTVV GGNDGVRDTW TPYGSPDMLE RAMLIGMRND FRRDDALEVA LDCVTHSAAR GCGFADYGLE AGKRADVVLV DALTFAEAVV ARPTRRLVVS SGR
Validated NT Sequence
tccgccccga cgacacgacg agccgacgcg tcggccgcgc gacgacggcc tcggcgaacg tcagcgcatc gacgagcacg acgtccgcac gcttgccggc ctccagcccg taatcggcga agccgcaacc acgcgccgcg ctgtgcgtca cgcagtcgag tgcgacttcg agcgcgtcgt cgcggcggaa atcgttgcgc atgccgatca gcatcgcgcg ttccagcatg tcgggcgaac cgtacggcgt ccacgtatcg cgcacgccgt cgttgccgcc gacgacggtc acgccggccg cccgacacgc gacgaccgac ggcacgggca ccgcggccgg cgcggtcgtg acgatcgcga cgccgagttc ggccatccgc gcgagcagcg cgtcgcgctc gcggccggca acgtcgccga ggcagaagcc gtggctgatc gtgaccttgc cttgcatgcc gagcgcggcc gtgcgctgca ggatcaggtc gagcgagtac gcgcccatcg acccgcgctc gtgcaggtgc aggtcgagcc cgcggccgtg gcgctcggcg atgccgaaca gcacgtccac cgcttcgacc ggatcgcctt cgatcgcgca cgggtcgagg ccgccgagca ggtcggcgcc cgcgccgagc gcgtcggaca gcagcgcggc ggtgccgggc cgcttgagca cgcccgactg cggaaacgcg acgatctgga tctccacctg gccgcgcagc gtctcgcgcg ttgcgagcac gccatgcaga tgccgcagcc cggcgtcggt gtcgacgtcg acgtgcgtgc ggatgcgcgt cgtgccggcc gcgaggaacg cgcgcgccag cgcgagcgac gcggcgcccg catcgtgacc gctcgtcgcg cggtagtggc gctcgttctc gatgcggtcg acgaggcgcg gccccaccgc gttgcgatac cacggcatgc cccagtgggt cttgtcgaga tgcgtgtggc cttcgacgag gccgggcagc gcaagcgcgt gcgcgccgtc ctcgatcgcg cagcccgccg gcgcgtcgag cgacgggccg acgcgcgcga tgcgctcgcc ttcgatcagg atgtcgagtg cttcgtcggc gtgcgtgcgg acgttgcgga tcagcaggtt cgtcatatgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHMT NLLIRNVRTH ADEALDILIE GERIARVGPS LDAPAGCAIE DGAHALALPG LVEGHTHLDK THWGMPWYRN AVGPRLVDRI ENERHYRATS GHDAGAASLA LARAFLAAGT TRIRTHVDVD TDAGLRHLHG VLATRETLRG QVEIQIVAFP QSGVLKRPGT AALLSDALGA GADLLGGLDP CAIEGDPVEA VDVLFGIAER HGRGLDLHLH ERGSMGAYSL DLILQRTAAL GMQGKVTISH GFCLGDVAGR ERDALLARMA ELGVAIVTTA PAAVPVPSVV ACRAAGVTVV GGNDGVRDTW TPYGSPDMLE RAMLIGMRND FRRDDALEVA LDCVTHSAAR GCGFADYGLE AGKRADVVLV DALTFAEAVV ARPTRRLVVS SGRIVARDGV LV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga acctgctgat ccgcaacgtc cgcacgcacg ccgacgaagc actcgacatc ctgatcgaag gcgagcgcat cgcgcgcgtc ggcccgtcgc tcgacgcgcc ggcgggctgc gcgatcgagg acggcgcgca cgcgcttgcg ctgcccggcc tcgtcgaagg ccacacgcat ctcgacaaga cccactgggg catgccgtgg tatcgcaacg cggtggggcc gcgcctcgtc gaccgcatcg agaacgagcg ccactaccgc gcgacgagcg gtcacgatgc gggcgccgcg tcgctcgcgc tggcgcgcgc gttcctcgcg gccggcacga cgcgcatccg cacgcacgtc gacgtcgaca ccgacgccgg gctgcggcat ctgcatggcg tgctcgcaac gcgcgagacg ctgcgcggcc aggtggagat ccagatcgtc gcgtttccgc agtcgggcgt gctcaagcgg cccggcaccg ccgcgctgct gtccgacgcg ctcggcgcgg gcgccgacct gctcggcggc ctcgacccgt gcgcgatcga aggcgatccg gtcgaagcgg tggacgtgct gttcggcatc gccgagcgcc acggccgcgg gctcgacctg cacctgcacg agcgcgggtc gatgggcgcg tactcgctcg acctgatcct gcagcgcacg gccgcgctcg gcatgcaagg caaggtcacg atcagccacg gcttctgcct cggcgacgtt gccggccgcg agcgcgacgc gctgctcgcg cggatggccg aactcggcgt cgcgatcgtc acgaccgcgc cggccgcggt gcccgtgccg tcggtcgtcg cgtgtcgggc ggccggcgtg accgtcgtcg gcggcaacga cggcgtgcgc gatacgtgga cgccgtacgg ttcgcccgac atgctggaac gcgcgatgct gatcggcatg cgcaacgatt tccgccgcga cgacgcgctc gaagtcgcac tcgactgcgt gacgcacagc gcggcgcgtg gttgcggctt cgccgattac gggctggagg ccggcaagcg tgcggacgtc gtgctcgtcg atgcgctgac gttcgccgag gccgtcgtcg cgcggccgac gcgtcggctc gtcgtgtcgt cggggcggat cgtcgcgcgc gacggcgtgc tggtctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for BuamA.00109.a.B1.PS37918
PURIFICATION DATe: 4/20/2016
CONCENTRATION: 20.5mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 9
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT NLLIRNVRTH ADEALDILIE GERIARVGPS LDAPAGCAIE DGAHALALPG LVEGHTHLDK THWGMPWYRN AVGPRLVDRI ENERHYRATS GHDAGAASLA LARAFLAAGT TRIRTHVDVD TDAGLRHLHG VLATRETLRG QVEIQIVAFP QSGVLKRPGT AALLSDALGA GADLLGGLDP CAIEGDPVEA VDVLFGIAER HGRGLDLHLH ERGSMGAYSL DLILQRTAAL GMQGKVTISH GFCLGDVAGR ERDALLARMA ELGVAIVTTA PAAVPVPSVV ACRAAGVTVV GGNDGVRDTW TPYGSPDMLE RAMLIGMRND FRRDDALEVA LDCVTHSAAR GCGFADYGLE AGKRADVVLV DALTFAEAVV ARPTRRLVVS SGR
Validated NT Sequence
tccgccccga cgacacgacg agccgacgcg tcggccgcgc gacgacggcc tcggcgaacg tcagcgcatc gacgagcacg acgtccgcac gcttgccggc ctccagcccg taatcggcga agccgcaacc acgcgccgcg ctgtgcgtca cgcagtcgag tgcgacttcg agcgcgtcgt cgcggcggaa atcgttgcgc atgccgatca gcatcgcgcg ttccagcatg tcgggcgaac cgtacggcgt ccacgtatcg cgcacgccgt cgttgccgcc gacgacggtc acgccggccg cccgacacgc gacgaccgac ggcacgggca ccgcggccgg cgcggtcgtg acgatcgcga cgccgagttc ggccatccgc gcgagcagcg cgtcgcgctc gcggccggca acgtcgccga ggcagaagcc gtggctgatc gtgaccttgc cttgcatgcc gagcgcggcc gtgcgctgca ggatcaggtc gagcgagtac gcgcccatcg acccgcgctc gtgcaggtgc aggtcgagcc cgcggccgtg gcgctcggcg atgccgaaca gcacgtccac cgcttcgacc ggatcgcctt cgatcgcgca cgggtcgagg ccgccgagca ggtcggcgcc cgcgccgagc gcgtcggaca gcagcgcggc ggtgccgggc cgcttgagca cgcccgactg cggaaacgcg acgatctgga tctccacctg gccgcgcagc gtctcgcgcg ttgcgagcac gccatgcaga tgccgcagcc cggcgtcggt gtcgacgtcg acgtgcgtgc ggatgcgcgt cgtgccggcc gcgaggaacg cgcgcgccag cgcgagcgac gcggcgcccg catcgtgacc gctcgtcgcg cggtagtggc gctcgttctc gatgcggtcg acgaggcgcg gccccaccgc gttgcgatac cacggcatgc cccagtgggt cttgtcgaga tgcgtgtggc cttcgacgag gccgggcagc gcaagcgcgt gcgcgccgtc ctcgatcgcg cagcccgccg gcgcgtcgag cgacgggccg acgcgcgcga tgcgctcgcc ttcgatcagg atgtcgagtg cttcgtcggc gtgcgtgcgg acgttgcgga tcagcaggtt cgtcatatgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHMT NLLIRNVRTH ADEALDILIE GERIARVGPS LDAPAGCAIE DGAHALALPG LVEGHTHLDK THWGMPWYRN AVGPRLVDRI ENERHYRATS GHDAGAASLA LARAFLAAGT TRIRTHVDVD TDAGLRHLHG VLATRETLRG QVEIQIVAFP QSGVLKRPGT AALLSDALGA GADLLGGLDP CAIEGDPVEA VDVLFGIAER HGRGLDLHLH ERGSMGAYSL DLILQRTAAL GMQGKVTISH GFCLGDVAGR ERDALLARMA ELGVAIVTTA PAAVPVPSVV ACRAAGVTVV GGNDGVRDTW TPYGSPDMLE RAMLIGMRND FRRDDALEVA LDCVTHSAAR GCGFADYGLE AGKRADVVLV DALTFAEAVV ARPTRRLVVS SGRIVARDGV LV
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA ACCTGCTGAT CCGCAACGTC CGCACGCACG CCGACGAAGC ACTCGACATC CTGATCGAAG GCGAGCGCAT CGCGCGCGTC GGCCCGTCGC TCGACGCGCC GGCGGGCTGC GCGATCGAGG ACGGCGCGCA CGCGCTTGCG CTGCCCGGCC TCGTCGAAGG CCACACGCAT CTCGACAAGA CCCACTGGGG CATGCCGTGG TATCGCAACG CGGTGGGGCC GCGCCTCGTC GACCGCATCG AGAACGAGCG CCACTACCGC GCGACGAGCG GTCACGATGC GGGCGCCGCG TCGCTCGCGC TGGCGCGCGC GTTCCTCGCG GCCGGCACGA CGCGCATCCG CACGCACGTC GACGTCGACA CCGACGCCGG GCTGCGGCAT CTGCATGGCG TGCTCGCAAC GCGCGAGACG CTGCGCGGCC AGGTGGAGAT CCAGATCGTC GCGTTTCCGC AGTCGGGCGT GCTCAAGCGG CCCGGCACCG CCGCGCTGCT GTCCGACGCG CTCGGCGCGG GCGCCGACCT GCTCGGCGGC CTCGACCCGT GCGCGATCGA AGGCGATCCG GTCGAAGCGG TGGACGTGCT GTTCGGCATC GCCGAGCGCC ACGGCCGCGG GCTCGACCTG CACCTGCACG AGCGCGGGTC GATGGGCGCG TACTCGCTCG ACCTGATCCT GCAGCGCACG GCCGCGCTCG GCATGCAAGG CAAGGTCACG ATCAGCCACG GCTTCTGCCT CGGCGACGTT GCCGGCCGCG AGCGCGACGC GCTGCTCGCG CGGATGGCCG AACTCGGCGT CGCGATCGTC ACGACCGCGC CGGCCGCGGT GCCCGTGCCG TCGGTCGTCG CGTGTCGGGC GGCCGGCGTG ACCGTCGTCG GCGGCAACGA CGGCGTGCGC GATACGTGGA CGCCGTACGG TTCGCCCGAC ATGCTGGAAC GCGCGATGCT GATCGGCATG CGCAACGATT TCCGCCGCGA CGACGCGCTC GAAGTCGCAC TCGACTGCGT GACGCACAGC GCGGCGCGTG GTTGCGGCTT CGCCGATTAC GGGCTGGAGG CCGGCAAGCG TGCGGACGTC GTGCTCGTCG ATGCGCTGAC GTTCGCCGAG GCCGTCGTCG CGCGGCCGAC GCGTCGGCTC GTCGTGTCGT CGGGGCGGAT CGTCGCGCGC GACGGCGTGC TGGTCTGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT