SSGCID: Target Page

BuamA.00107.b

Efflux transporter, RND family, MFP subunit

CENTER ID:	BuamA.00107.b
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00107.b.A2.GE32132	Mature protein( BuamA.00107.b )		24	432

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00107.b.A2.PW32050	Mature protein( BuamA.00107.b )		24	432

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.2601
RefSeq:	YP_001809222.1
UniProt:	B1YVU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MRVERVPYRL ITVAAAAVFL AACGKKESAP PPQTPEVGVV TVQPQAVPVF TELPGRTSAF LVAQVRARVD GIVLRREFTE GGDVKAGQRL YKIDPAPYIA QLNSAKATLA KAQANLVTQN ALVARYKVLV AANAVSKQEY DNAVATQGQA AADVAAGKAA VDTAQINLGY TDVVSPISGR VGISQVTPGA YVQASQATLM STVQQLDPVY VDLTQSSLDG LKLRQDVQSG RLKTSGPGAA KVSLILEDGK TYSDAGKLQF SDVTVDQTTG SVTIRAVFPN PGRVLLPGMF VRARIEEGVN ENAFLVPQIG VTHDAKGQPV ALVVNASNKV EPRPLKTTGM QGQNWIVEGG LQAGDRVIVQ GVEKVRPGAT VKSVAAQLPA ADAASGAAVS AAPAAAASGA AVSGAGASGA AASGAAASGA APASAAAASS AK

NT Sequence

ATGCGCGTCG AACGGGTTCC ATACCGCTTG ATCACTGTCG CGGCAGCCGC GGTTTTCCTG GCTGCCTGCG GAAAAAAAGA ATCGGCACCG CCGCCGCAAA CGCCGGAAGT CGGTGTCGTC ACCGTCCAGC CGCAAGCCGT CCCGGTCTTC ACCGAACTGC CGGGCCGCAC CAGCGCCTTC CTCGTCGCGC AAGTGCGTGC ACGGGTCGAC GGCATCGTGC TGCGCCGTGA ATTCACCGAA GGCGGCGACG TCAAGGCCGG CCAGCGCCTC TACAAGATCG ACCCGGCACC GTACATCGCT CAACTGAACA GCGCGAAGGC GACGCTCGCG AAGGCGCAGG CGAACCTCGT CACGCAGAAC GCGCTCGTCG CGCGCTACAA GGTGCTGGTG GCCGCGAACG CGGTCAGCAA GCAGGAATAC GACAACGCGG TGGCCACGCA AGGCCAGGCC GCGGCGGACG TCGCGGCCGG CAAGGCAGCG GTCGATACCG CGCAGATCAA CCTCGGCTAT ACGGACGTCG TGTCGCCGAT CAGCGGCCGC GTCGGCATTT CGCAGGTCAC GCCGGGCGCC TACGTGCAGG CGAGCCAGGC GACGTTGATG TCGACGGTGC AGCAGCTCGA TCCGGTCTAT GTCGACCTGA CGCAGTCGAG CCTCGACGGG CTGAAGCTGC GCCAGGACGT GCAGAGCGGC CGCCTGAAGA CGAGCGGCCC GGGCGCGGCG AAGGTGTCGC TGATCCTCGA GGACGGCAAG ACCTATTCCG ACGCCGGCAA GCTGCAGTTT TCCGACGTCA CGGTCGACCA GACGACGGGC TCGGTGACGA TCCGCGCGGT ATTCCCGAAC CCGGGCCGCG TGCTGCTGCC CGGGATGTTC GTGCGCGCAC GGATCGAGGA AGGCGTGAAC GAGAACGCGT TCCTGGTGCC GCAGATCGGC GTCACGCATG ACGCGAAGGG CCAGCCGGTC GCGCTGGTCG TGAACGCGAG CAACAAGGTC GAGCCGCGTC CGCTGAAGAC GACCGGCATG CAAGGTCAGA ACTGGATCGT CGAAGGCGGC CTGCAAGCGG GCGACCGCGT GATCGTGCAG GGCGTCGAGA AGGTTCGTCC GGGCGCGACG GTGAAGTCGG TCGCCGCGCA GTTGCCGGCC GCCGACGCCG CATCGGGTGC AGCCGTATCC GCCGCGCCGG CCGCTGCCGC GTCGGGCGCC GCTGTGTCGG GCGCTGGTGC GTCGGGTGCT GCCGCTTCGG GTGCCGCCGC ATCGGGCGCA GCGCCGGCGA GCGCCGCCGC CGCTTCGAGC GCGAAA

Details for BuamA.00107.b.A2.GE32132

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	43kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	13
PERCENT COVERAGE:	57

Validated AA Sequence

MXXSHVXXHX XXXXXXXXXK XXXXXGPGSM GXXXXXXXXX XXXXXXXXXP XAXAXXXXXL AAAXADXXXX XXXXXXXXXE FXERGXVXAG XXXXXIXPXX XXAXXXXXXX XXXKXXXNLX XXXXLXXXXR XXXPXXXXXX XTTTXXXXXX XXRXXXXXXX XXXXXXXSPX XXXXVXSXIX XXXXFXGXXX XLXXXXXXXX XXXXXXXXLX XSELSXSXXX GXXXXXXXXX XXXXXXXAXR NXXXXXX

Validated NT Sequence

atgcnnnnca gccatgttta nnnncactnn nnnnnnannn acannnnnnn anaannnaaa nnnannannn annnnggtcc tggttcgatg ggnannnnag nancgnnncn ggnnncnnan nnnnnnnnnn ccnnnnnnnn nnnnnatccn nctgcnnncg cccnnnnngn anctganttg gctgctgccn ccgctgacnn ngnnnnnnan nnnntcnnnn ncntcnngnn nngccnngaa ttcnccgaac gcggnnacgt nnaggccggc nannnncnnn ncannatcna nccgnnnnnn nanntcgctc annngannan cnncnnnnnn annnnngnna aggngnnngn gaacctcntc angnanaang ngctnnnnng cgntanaagg tgnnggnggc cgnnaangtn ancnnnnngn natacnacaa cgnnnnnnnc nnncnnngnn nnnnngcggn tganctnnnn nncngnnnng nnnnggntga nanngntcan atctcccnna tnntannngn nngtcntgtc gnnnatcagn tnnnnnncnn nntttnnagg nngnncnnnn ngtctntnnn nnntgnnnnc nngnnnnnnn nngnannnna nannnnntng atnnttgtnt ntgtcngagc tntcgnantc ganannnnnn ggntgnannn gnncnncnnc nngnnanngg nnnnnnngcn nnnnntnncn ngcccngagg aatcnnnnnn nnannnnnna tgga

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGKKESAPP PQTPEVGVVT VQPQAVPVFT ELPGRTSAFL VAQVRARVDG IVLRREFTEG GDVKAGQRLY KIDPAPYIAQ LNSAKATLAK AQANLVTQNA LVARYKVLVA ANAVSKQEYD NAVATQGQAA ADVAAGKAAV DTAQINLGYT DVVSPISGRV GISQVTPGAY VQASQATLMS TVQQLDPVYV DLTQSSLDGL KLRQDVQSGR LKTSGPGAAK VSLILEDGKT YSDAGKLQFS DVTVDQTTGS VTIRAVFPNP GRVLLPGMFV RARIEEGVNE NAFLVPQIGV THDAKGQPVA LVVNASNKVE PRPLKTTGMQ GQNWIVEGGL QAGDRVIVQG VEKVRPGATV KSVAAQLPAA DAASGAAVSA APAAAASGAA VSGAGASGAA ASGAAASGAA PASAAAASSA K

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggaaaaaaa gaatcggcac cgccgccgca aacgccggaa gtcggtgtcg tcaccgtcca gccgcaagcc gtcccggtct tcaccgaact gccgggccgc accagcgcct tcctcgtcgc gcaagtgcgt gcacgggtcg acggcatcgt gctgcgccgt gaattcaccg aaggcggcga cgtcaaggcc ggccagcgcc tctacaagat cgacccggca ccgtacatcg ctcaactgaa cagcgcgaag gcgacgctcg cgaaggcgca ggcgaacctc gtcacgcaga acgcgctcgt cgcgcgctac aaggtgctgg tggccgcgaa cgcggtcagc aagcaggaat acgacaacgc ggtggccacg caaggccagg ccgcggcgga cgtcgcggcc ggcaaggcag cggtcgatac cgcgcagatc aacctcggct atacggacgt cgtgtcgccg atcagcggcc gcgtcggcat ttcgcaggtc acgccgggcg cctacgtgca ggcgagccag gcgacgttga tgtcgacggt gcagcagctc gatccggtct atgtcgacct gacgcagtcg agcctcgacg ggctgaagct gcgccaggac gtgcagagcg gccgcctgaa gacgagcggc ccgggcgcgg cgaaggtgtc gctgatcctc gaggacggca agacctattc cgacgccggc aagctgcagt tttccgacgt cacggtcgac cagacgacgg gctcggtgac gatccgcgcg gtattcccga acccgggccg cgtgctgctg cccgggatgt tcgtgcgcgc acggatcgag gaaggcgtga acgagaacgc gttcctggtg ccgcagatcg gcgtcacgca tgacgcgaag ggccagccgg tcgcgctggt cgtgaacgcg agcaacaagg tcgagccgcg tccgctgaag acgaccggca tgcaaggtca gaactggatc gtcgaaggcg gcctgcaagc gggcgaccgc gtgatcgtgc agggcgtcga gaaggttcgt ccgggcgcga cggtgaagtc ggtcgccgcg cagttgccgg ccgccgacgc cgcatcgggt gcagccgtat ccgccgcgcc ggccgctgcc gcgtcgggcg ccgctgtgtc gggcgctggt gcgtcgggtg ctgccgcttc gggtgccgcc gcatcgggcg cagcgccggc gagcgccgcc gccgcttcga gcgcgaaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BuamA.00107.b.A2.PW32050

PURIFICATION DATe:	5/30/2011
CONCENTRATION:	23.22mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	12
VIAL VOLUME:	100µl
PERCENT IDENTITY:	13
PERCENT COVERAGE:	57

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGAAAAAAA GAATCGGCAC CGCCGCCGCA AACGCCGGAA GTCGGTGTCG TCACCGTCCA GCCGCAAGCC GTCCCGGTCT TCACCGAACT GCCGGGCCGC ACCAGCGCCT TCCTCGTCGC GCAAGTGCGT GCACGGGTCG ACGGCATCGT GCTGCGCCGT GAATTCACCG AAGGCGGCGA CGTCAAGGCC GGCCAGCGCC TCTACAAGAT CGACCCGGCA CCGTACATCG CTCAACTGAA CAGCGCGAAG GCGACGCTCG CGAAGGCGCA GGCGAACCTC GTCACGCAGA ACGCGCTCGT CGCGCGCTAC AAGGTGCTGG TGGCCGCGAA CGCGGTCAGC AAGCAGGAAT ACGACAACGC GGTGGCCACG CAAGGCCAGG CCGCGGCGGA CGTCGCGGCC GGCAAGGCAG CGGTCGATAC CGCGCAGATC AACCTCGGCT ATACGGACGT CGTGTCGCCG ATCAGCGGCC GCGTCGGCAT TTCGCAGGTC ACGCCGGGCG CCTACGTGCA GGCGAGCCAG GCGACGTTGA TGTCGACGGT GCAGCAGCTC GATCCGGTCT ATGTCGACCT GACGCAGTCG AGCCTCGACG GGCTGAAGCT GCGCCAGGAC GTGCAGAGCG GCCGCCTGAA GACGAGCGGC CCGGGCGCGG CGAAGGTGTC GCTGATCCTC GAGGACGGCA AGACCTATTC CGACGCCGGC AAGCTGCAGT TTTCCGACGT CACGGTCGAC CAGACGACGG GCTCGGTGAC GATCCGCGCG GTATTCCCGA ACCCGGGCCG CGTGCTGCTG CCCGGGATGT TCGTGCGCGC ACGGATCGAG GAAGGCGTGA ACGAGAACGC GTTCCTGGTG CCGCAGATCG GCGTCACGCA TGACGCGAAG GGCCAGCCGG TCGCGCTGGT CGTGAACGCG AGCAACAAGG TCGAGCCGCG TCCGCTGAAG ACGACCGGCA TGCAAGGTCA GAACTGGATC GTCGAAGGCG GCCTGCAAGC GGGCGACCGC GTGATCGTGC AGGGCGTCGA GAAGGTTCGT CCGGGCGCGA CGGTGAAGTC GGTCGCCGCG CAGTTGCCGG CCGCCGACGC CGCATCGGGT GCAGCCGTAT CCGCCGCGCC GGCCGCTGCC GCGTCGGGCG CCGCTGTGTC GGGCGCTGGT GCGTCGGGTG CTGCCGCTTC GGGTGCCGCC GCATCGGGCG CAGCGCCGGC GAGCGCCGCC GCCGCTTCGA GCGCGAAATA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A