SSGCID: Target Page

BuamA.00107.a

Efflux transporter, RND family, MFP subunit

CENTER ID:	BuamA.00107.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00107.a.A2.GE32127	Mature protein( BuamA.00107.a )		27	395

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00107.a.A2.PW32045	Mature protein( BuamA.00107.a )		27	395

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.1581
RefSeq:	YP_001808246.1
UniProt:	B1YQ92

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNNKRSLARR LQLAPVAFAA MLAVAGCGKG DNDKAPEAIQ NATVVTVRPT AVPMTVELPG RLDAYRQAPV RARVAGIVTA RTYEEGQEVK QGAVLFRIDP APLKAARDAA QGALAKAQAA ALAASDKRRR YDDLVRDRAV SERDHTEALA DDTRAKADVA SAKAELARAQ LQLDYATVTA PIAGRARRAL VTEGALVGQD QATPLTTVEQ LDPIYVNFSQ PAADVDALRR AVKSGHATGI AQHDVTVTLR RADGTAYPLK GKLLFSDLAV DPTTDTVAMR ALFPNPEREL LPGAYVRIAL DAAVDQRAIL VPRDALLRTA ERTSVRVVGT NGKVKDVEVV ADQMSGRDWR ITRGLSGGER VIVDNAAQFA PDTAVKPVEQ ASPTKAASAA AARQT

NT Sequence

ATGAATAACA AACGCTCCCT CGCGCGCCGC CTCCAGCTGG CGCCCGTCGC GTTCGCGGCC ATGCTGGCCG TGGCCGGATG CGGAAAGGGC GACAACGACA AGGCGCCGGA GGCCATTCAG AACGCGACGG TCGTGACGGT GCGTCCGACG GCCGTGCCGA TGACCGTCGA ATTGCCGGGC CGGCTCGACG CGTATCGGCA AGCGCCGGTG CGCGCACGGG TCGCGGGCAT CGTCACCGCG CGGACTTACG AGGAAGGCCA GGAAGTGAAG CAGGGCGCGG TGCTGTTCCG CATCGATCCC GCGCCGCTGA AGGCCGCGCG CGATGCCGCG CAGGGTGCGC TCGCGAAGGC GCAGGCCGCC GCGCTCGCGG CGAGCGACAA GCGCCGCCGT TACGACGACC TGGTGCGCGA CCGCGCGGTC AGCGAGCGCG ATCACACCGA GGCTCTCGCC GACGACACGA GGGCTAAGGC CGACGTCGCG TCCGCGAAGG CCGAGCTCGC ACGCGCGCAG TTGCAGCTCG ACTATGCGAC CGTCACCGCG CCGATTGCGG GCCGCGCACG ACGCGCGCTC GTCACCGAAG GCGCGCTCGT CGGCCAGGAC CAGGCCACGC CGCTCACGAC CGTCGAGCAG CTCGATCCGA TCTACGTGAA TTTCTCGCAG CCGGCGGCCG ACGTCGACGC GTTGCGTCGC GCGGTGAAGA GCGGGCACGC GACGGGGATC GCGCAGCACG ACGTCACGGT GACGCTGCGG CGCGCCGACG GCACCGCGTA TCCGCTGAAG GGCAAGCTGC TGTTCAGCGA TCTCGCGGTC GATCCGACCA CCGATACCGT CGCGATGCGC GCGCTGTTCC CCAACCCCGA GCGCGAGCTG CTGCCCGGCG CGTATGTGCG GATCGCGCTC GATGCGGCCG TCGACCAGCG GGCGATCCTC GTGCCGCGCG ATGCGCTGCT GCGCACCGCC GAGCGCACGT CGGTGCGCGT GGTCGGCACG AACGGCAAGG TGAAGGACGT CGAGGTCGTC GCCGACCAGA TGAGCGGACG CGACTGGCGC ATCACGCGCG GCCTGTCGGG CGGCGAGCGC GTGATCGTCG ACAACGCCGC GCAGTTCGCG CCCGATACCG CCGTCAAGCC CGTCGAGCAG GCATCGCCGA CGAAGGCCGC GTCAGCCGCG GCCGCCCGTC AAACC

Details for BuamA.00107.a.A2.GE32127

HARVESTED ON:	4/26/2011
SEQUENCED ON:	5/2/2011
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	91

Validated AA Sequence

MCGKGDNDKA PEAIQNATVV TVRPTAVPMT VELPGRLDXY RQAPVRARVA GIVTARTYEE GQEVKQGAVL FRIDPAPLKA ARDAAQGALA KAQAAALAAS DKRRRYDDLV RDRAVSERDH TEALADDTRA KADVASAKAE LARAQLQLDY ATVTAPIAGR ARRALVTEGA LVGQDQATPL TTVEQLDPIY VNFSQPAADV DALRRAVKSG HATGIAQHDV TVTLRRADGT AYPLKGKLLF SDLAVDPTTD TVAMRALFPN PERELLPGAY VRIALDAAVD QRAILVPRDA LLRTAERTSV RVVGTNGKVK DVEVVADQMS GRDWRITRGL SGGERVIVDN AAQFAPDTAV KPVEQASP

Validated NT Sequence

tcggcgatgc ctgctcgacg ggcttgacgg cggtatcggg cgcgaactgc gcggcgttgt cgacgatcac gcgctcgccg cccgacaggc cgcgcgtgat gcgccagtcg cgtccgctca tctggtcggc gacgacctcg acgtccttca ccttgccgtt cgtgccgacc acgcgcaccg acgtgcgctc ggcggtgcgc agcagcgcat cgcgcggcac gaggatcgcc cgctggtcga cggccgcatc gagcgcgatc cgcacatacg cgccgggcag cagctcgcgc tcggggttgg ggaacagcgc gcgcatcgcg acggtatcgg tggtcggatc gaccgcgaga tcgctgaaca gcagcttgcc cttcagcgga tacgcggtgc cgtcggcgcg ccgcagcgtc accgtgacgt cgtgctgcgc gatccccgtc gcgtgcccgc tcttcaccgc gcgacgcaac gcgtcgacgt cggccgccgg ctgcgagaaa ttcacgtaga tcggatcgag ctgctcgacg gtcgtgagcg gcgtggcctg gtcctggccg acgagcgcgc cttcggtgac gagcgcgcgt cgtgcgcggc ccgcaatcgg cgcggtgacg gtcgcatagt cgagctgcaa ctgcgcgcgt gcgagctcgg ccttcgcgga cgcgacgtcg gccttagccc tcgtgtcgtc ggcgagagcc tcggtgtgat cgcgctcgct gaccgcgcgg tcgcgcacca ggtcgtcgta acggcggcgc ttgtcgctcg ccgcgagcgc ggcggcctgc gccttcgcga gcgcaccctg cgcggcatcg cgcgcggcct tcagcggcgc gggatcgatg cggaacagca ccgcgccctg cttcacttcc tggccttcct cgtaagtccg cgcggtgacg atgcccgcga cccgtgcgcg caccggcgct tgccgatacg ngtcgagccg gcccggcaat tcgacggtca tcggcacggc cgtcggacgc accgtcacga ccgtcgcgtt ctgaatggcc tccggcgcct tgtcgttgtc gccctttccg cacatcgaac caggaccc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMCGKGDNDK APEAIQNATV VTVRPTAVPM TVELPGRLDA YRQAPVRARV AGIVTARTYE EGQEVKQGAV LFRIDPAPLK AARDAAQGAL AKAQAAALAA SDKRRRYDDL VRDRAVSERD HTEALADDTR AKADVASAKA ELARAQLQLD YATVTAPIAG RARRALVTEG ALVGQDQATP LTTVEQLDPI YVNFSQPAAD VDALRRAVKS GHATGIAQHD VTVTLRRADG TAYPLKGKLL FSDLAVDPTT DTVAMRALFP NPERELLPGA YVRIALDAAV DQRAILVPRD ALLRTAERTS VRVVGTNGKV KDVEVVADQM SGRDWRITRG LSGGERVIVD NAAQFAPDTA VKPVEQASPT KAASAAAARQ T

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcggaaag ggcgacaacg acaaggcgcc ggaggccatt cagaacgcga cggtcgtgac ggtgcgtccg acggccgtgc cgatgaccgt cgaattgccg ggccggctcg acgcgtatcg gcaagcgccg gtgcgcgcac gggtcgcggg catcgtcacc gcgcggactt acgaggaagg ccaggaagtg aagcagggcg cggtgctgtt ccgcatcgat cccgcgccgc tgaaggccgc gcgcgatgcc gcgcagggtg cgctcgcgaa ggcgcaggcc gccgcgctcg cggcgagcga caagcgccgc cgttacgacg acctggtgcg cgaccgcgcg gtcagcgagc gcgatcacac cgaggctctc gccgacgaca cgagggctaa ggccgacgtc gcgtccgcga aggccgagct cgcacgcgcg cagttgcagc tcgactatgc gaccgtcacc gcgccgattg cgggccgcgc acgacgcgcg ctcgtcaccg aaggcgcgct cgtcggccag gaccaggcca cgccgctcac gaccgtcgag cagctcgatc cgatctacgt gaatttctcg cagccggcgg ccgacgtcga cgcgttgcgt cgcgcggtga agagcgggca cgcgacgggg atcgcgcagc acgacgtcac ggtgacgctg cggcgcgccg acggcaccgc gtatccgctg aagggcaagc tgctgttcag cgatctcgcg gtcgatccga ccaccgatac cgtcgcgatg cgcgcgctgt tccccaaccc cgagcgcgag ctgctgcccg gcgcgtatgt gcggatcgcg ctcgatgcgg ccgtcgacca gcgggcgatc ctcgtgccgc gcgatgcgct gctgcgcacc gccgagcgca cgtcggtgcg cgtggtcggc acgaacggca aggtgaagga cgtcgaggtc gtcgccgacc agatgagcgg acgcgactgg cgcatcacgc gcggcctgtc gggcggcgag cgcgtgatcg tcgacaacgc cgcgcagttc gcgcccgata ccgccgtcaa gcccgtcgag caggcatcgc cgacgaaggc cgcgtcagcc gcggccgccc gtcaaaccta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BuamA.00107.a.A2.PW32045

PURIFICATION DATe:	5/30/2011
CONCENTRATION:	37.51mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	18
VIAL VOLUME:	100µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	91

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGCGGAAAG GGCGACAACG ACAAGGCGCC GGAGGCCATT CAGAACGCGA CGGTCGTGAC GGTGCGTCCG ACGGCCGTGC CGATGACCGT CGAATTGCCG GGCCGGCTCG ACGCGTATCG GCAAGCGCCG GTGCGCGCAC GGGTCGCGGG CATCGTCACC GCGCGGACTT ACGAGGAAGG CCAGGAAGTG AAGCAGGGCG CGGTGCTGTT CCGCATCGAT CCCGCGCCGC TGAAGGCCGC GCGCGATGCC GCGCAGGGTG CGCTCGCGAA GGCGCAGGCC GCCGCGCTCG CGGCGAGCGA CAAGCGCCGC CGTTACGACG ACCTGGTGCG CGACCGCGCG GTCAGCGAGC GCGATCACAC CGAGGCTCTC GCCGACGACA CGAGGGCTAA GGCCGACGTC GCGTCCGCGA AGGCCGAGCT CGCACGCGCG CAGTTGCAGC TCGACTATGC GACCGTCACC GCGCCGATTG CGGGCCGCGC ACGACGCGCG CTCGTCACCG AAGGCGCGCT CGTCGGCCAG GACCAGGCCA CGCCGCTCAC GACCGTCGAG CAGCTCGATC CGATCTACGT GAATTTCTCG CAGCCGGCGG CCGACGTCGA CGCGTTGCGT CGCGCGGTGA AGAGCGGGCA CGCGACGGGG ATCGCGCAGC ACGACGTCAC GGTGACGCTG CGGCGCGCCG ACGGCACCGC GTATCCGCTG AAGGGCAAGC TGCTGTTCAG CGATCTCGCG GTCGATCCGA CCACCGATAC CGTCGCGATG CGCGCGCTGT TCCCCAACCC CGAGCGCGAG CTGCTGCCCG GCGCGTATGT GCGGATCGCG CTCGATGCGG CCGTCGACCA GCGGGCGATC CTCGTGCCGC GCGATGCGCT GCTGCGCACC GCCGAGCGCA CGTCGGTGCG CGTGGTCGGC ACGAACGGCA AGGTGAAGGA CGTCGAGGTC GTCGCCGACC AGATGAGCGG ACGCGACTGG CGCATCACGC GCGGCCTGTC GGGCGGCGAG CGCGTGATCG TCGACAACGC CGCGCAGTTC GCGCCCGATA CCGCCGTCAA GCCCGTCGAG CAGGCATCGC CGACGAAGGC CGCGTCAGCC GCGGCCGCCC GTCAAACCTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A