BuamA.00104.a
Beta-lactamase
| CENTER ID: | BuamA.00104.a |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00104.a.B2.GE39259 | Mature protein( BuamA.00104.a ) | 39 | 299 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00104.a.B2.PS02499 | Mature protein( BuamA.00104.a ) | 39 | 299 |
Structures
5IHV
DEPOSITED: 2/29/2016
DETERMINATION: XRay
CLONE: BuamA.00104.a.B2.GE39259
PROTEIN: BuamA.00104.a.B2.PS02499
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_143180 |
| PATRIC ID: | fig|398577.6.peg.4650 |
| RefSeq: | YP_001811159.1 |
| UniProt: | B1YX81 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MEYSPTRRSL LLAAVAAPFV AACSSAPVAG QVHAHAAQAE LAALEKASNG RLGIAVLDTS NGTRIAHHAR ERFPLCGTYA VVAAAAILAR GSLDASLLPR RILYRRYEVV AGSPVTESHV DTGMTIAQLC AAMLQSGDKG AGNLLMRVLG GPQAVTSFAH ESGDTVFRLD RWEPELNLAA PGDERDTSTP VAMVDLLQQL LLGDTLREPQ RAQLTEWMTG GARGATAIAA GVPPGWRVAD KAGTGGYGTT TDVAVLWPPS RAPIVLAVSF TQPRADAAAR ADVVASAARI ATGALTATA
NT Sequence
ATGGAATATT CTCCGACACG CCGCTCGCTG TTGCTCGCCG CCGTCGCCGC ACCGTTCGTC GCCGCGTGCT CGTCGGCGCC CGTCGCCGGC CAGGTGCACG CCCATGCCGC CCAGGCCGAA CTCGCCGCGC TCGAAAAGGC ATCGAACGGC CGGCTCGGTA TCGCCGTACT CGATACGTCG AACGGCACGC GCATCGCGCA TCACGCGCGC GAGCGCTTCC CGCTGTGCGG CACCTATGCG GTCGTTGCGG CCGCCGCGAT CCTCGCGCGC GGTTCGCTCG ACGCATCGCT GCTGCCGCGC CGCATCCTGT ATCGCCGCTA TGAAGTCGTG GCCGGCTCGC CGGTGACGGA AAGTCATGTC GACACGGGGA TGACGATCGC GCAGCTGTGC GCGGCGATGC TGCAGTCGGG CGACAAGGGC GCCGGCAACC TGCTGATGCG CGTGCTCGGC GGCCCGCAGG CCGTCACGTC GTTCGCGCAT GAAAGCGGCG ACACCGTGTT CCGGCTCGAC CGCTGGGAAC CCGAACTGAA CCTGGCCGCG CCCGGCGACG AGCGCGATAC GTCGACGCCG GTCGCGATGG TCGACCTGCT GCAGCAACTG CTGCTCGGCG ATACGCTGCG CGAGCCGCAG CGTGCGCAGC TCACCGAATG GATGACCGGC GGCGCGCGCG GCGCGACCGC GATCGCGGCC GGCGTGCCGC CCGGCTGGCG TGTCGCGGAC AAGGCCGGCA CCGGCGGCTA CGGCACGACG ACCGATGTCG CGGTGCTGTG GCCGCCGTCG CGCGCGCCGA TCGTGCTGGC CGTGTCGTTC ACGCAGCCGC GTGCCGACGC CGCGGCCCGC GCGGATGTCG TCGCGTCGGC GGCGCGCATC GCGACGGGCG CGCTCACCGC AACGGCC
Details for BuamA.00104.a.B2.GE39259
| HARVESTED ON: | 6/10/2015 |
| SEQUENCED ON: | 6/5/2015 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHHM AELAALEKAS NGRLGIAVLD TSNGTRIAHH ARERFPLCGT YAVVAAAAIL ARGSLDASLL PRRILYRRYE VVAGSPVTES HVDTGMTIAQ LCAAMLQSGD KGAGNLLMRV LGGPQAVTSF AHESGDTVFR LDRWEPELNL AAPGDERDTS TPVAMVDLLQ QLLLGDTLRE PQRAQLTEWM TGGARGATAI AAGVPPGWRV ADKAGTGGYG TTTDVAVLWP PSRAPIVLAV SFTQPRADAA ARADVVASAA RIAXXXLTAT A
Validated NT Sequence
tcacttaggc cgttgcggtg agcgnnnnnn tcgcgatgcg cgccgccgac gcgacgacat ccgcgcgggc cgcggcgtcg gcacgcggct gcgtgaacga cacggccagc acgatcggcg cgcgcgacgg cggccacagc accgcgacat cggtcgtcgt gccgtagccg ccggtgccgg ccttgtccgc gacacgccag ccgggcggca cgccggccgc gatcgcggtc gcgccgcgcg cgccgccggt catccattcg gtgagctgcg cacgctgcgg ctcgcgcagc gtatcgccga gcagcagttg ctgcagcagg tcgaccatcg cgaccggcgt cgacgtatcg cgctcgtcgc cgggcgcggc caggttcagt tcgggttccc agcggtcgag ccggaacacg gtgtcgccgc tttcatgcgc gaacgacgtg acggcctgcg ggccgccgag cacgcgcatc agcaggttgc cggcgccctt gtcgcccgac tgcagcatcg ccgcgcacag ctgcgcgatc gtcatccccg tgtcgacatg actttccgtc accggcgagc cggccacgac ttcatagcgg cgatacagga tgcggcgcgg cagcagcgat gcgtcgagcg aaccgcgcgc gaggatcgcg gcggccgcaa cgaccgcata ggtgccgcac agcgggaagc gctcgcgcgc gtgatgcgcg atgcgcgtgc cgttcgacgt atcgagtacg gcgataccga gccggccgtt cgatgccttt tcgagcgcgg cgagttcggc catatggtgg tggtggtggt ggtgagccat
Expected Protein Sequence
MAHHHHHHAE LAALEKASNG RLGIAVLDTS NGTRIAHHAR ERFPLCGTYA VVAAAAILAR GSLDASLLPR RILYRRYEVV AGSPVTESHV DTGMTIAQLC AAMLQSGDKG AGNLLMRVLG GPQAVTSFAH ESGDTVFRLD RWEPELNLAA PGDERDTSTP VAMVDLLQQL LLGDTLREPQ RAQLTEWMTG GARGATAIAA GVPPGWRVAD KAGTGGYGTT TDVAVLWPPS RAPIVLAVSF TQPRADAAAR ADVVASAARI ATGALTATA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccgaac tcgccgcgct cgaaaaggca tcgaacggcc ggctcggtat cgccgtactc gatacgtcga acggcacgcg catcgcgcat cacgcgcgcg agcgcttccc gctgtgcggc acctatgcgg tcgttgcggc cgccgcgatc ctcgcgcgcg gttcgctcga cgcatcgctg ctgccgcgcc gcatcctgta tcgccgctat gaagtcgtgg ccggctcgcc ggtgacggaa agtcatgtcg acacggggat gacgatcgcg cagctgtgcg cggcgatgct gcagtcgggc gacaagggcg ccggcaacct gctgatgcgc gtgctcggcg gcccgcaggc cgtcacgtcg ttcgcgcatg aaagcggcga caccgtgttc cggctcgacc gctgggaacc cgaactgaac ctggccgcgc ccggcgacga gcgcgatacg tcgacgccgg tcgcgatggt cgacctgctg cagcaactgc tgctcggcga tacgctgcgc gagccgcagc gtgcgcagct caccgaatgg atgaccggcg gcgcgcgcgg cgcgaccgcg atcgcggccg gcgtgccgcc cggctggcgt gtcgcggaca aggccggcac cggcggctac ggcacgacga ccgatgtcgc ggtgctgtgg ccgccgtcgc gcgcgccgat cgtgctggcc gtgtcgttca cgcagccgcg tgccgacgcc gcggcccgcg cggatgtcgt cgcgtcggcg gcgcgcatcg cgacgggcgc gctcaccgca acggcctgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for BuamA.00104.a.B2.PS02499
| PURIFICATION DATe: | 8/27/2015 |
| CONCENTRATION: | 48.83mg/ml |
| OBSERVED MW: | 28kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 300 mM Nacl, 20 mM Hepes, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHM AELAALEKAS NGRLGIAVLD TSNGTRIAHH ARERFPLCGT YAVVAAAAIL ARGSLDASLL PRRILYRRYE VVAGSPVTES HVDTGMTIAQ LCAAMLQSGD KGAGNLLMRV LGGPQAVTSF AHESGDTVFR LDRWEPELNL AAPGDERDTS TPVAMVDLLQ QLLLGDTLRE PQRAQLTEWM TGGARGATAI AAGVPPGWRV ADKAGTGGYG TTTDVAVLWP PSRAPIVLAV SFTQPRADAA ARADVVASAA RIAXXXLTAT A
Validated NT Sequence
tcacttaggc cgttgcggtg agcgnnnnnn tcgcgatgcg cgccgccgac gcgacgacat ccgcgcgggc cgcggcgtcg gcacgcggct gcgtgaacga cacggccagc acgatcggcg cgcgcgacgg cggccacagc accgcgacat cggtcgtcgt gccgtagccg ccggtgccgg ccttgtccgc gacacgccag ccgggcggca cgccggccgc gatcgcggtc gcgccgcgcg cgccgccggt catccattcg gtgagctgcg cacgctgcgg ctcgcgcagc gtatcgccga gcagcagttg ctgcagcagg tcgaccatcg cgaccggcgt cgacgtatcg cgctcgtcgc cgggcgcggc caggttcagt tcgggttccc agcggtcgag ccggaacacg gtgtcgccgc tttcatgcgc gaacgacgtg acggcctgcg ggccgccgag cacgcgcatc agcaggttgc cggcgccctt gtcgcccgac tgcagcatcg ccgcgcacag ctgcgcgatc gtcatccccg tgtcgacatg actttccgtc accggcgagc cggccacgac ttcatagcgg cgatacagga tgcggcgcgg cagcagcgat gcgtcgagcg aaccgcgcgc gaggatcgcg gcggccgcaa cgaccgcata ggtgccgcac agcgggaagc gctcgcgcgc gtgatgcgcg atgcgcgtgc cgttcgacgt atcgagtacg gcgataccga gccggccgtt cgatgccttt tcgagcgcgg cgagttcggc catatggtgg tggtggtggt ggtgagccat
Expressed Protein Sequence
MAHHHHHHAE LAALEKASNG RLGIAVLDTS NGTRIAHHAR ERFPLCGTYA VVAAAAILAR GSLDASLLPR RILYRRYEVV AGSPVTESHV DTGMTIAQLC AAMLQSGDKG AGNLLMRVLG GPQAVTSFAH ESGDTVFRLD RWEPELNLAA PGDERDTSTP VAMVDLLQQL LLGDTLREPQ RAQLTEWMTG GARGATAIAA GVPPGWRVAD KAGTGGYGTT TDVAVLWPPS RAPIVLAVSF TQPRADAAAR ADVVASAARI ATGALTATA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGAAC TCGCCGCGCT CGAAAAGGCA TCGAACGGCC GGCTCGGTAT CGCCGTACTC GATACGTCGA ACGGCACGCG CATCGCGCAT CACGCGCGCG AGCGCTTCCC GCTGTGCGGC ACCTATGCGG TCGTTGCGGC CGCCGCGATC CTCGCGCGCG GTTCGCTCGA CGCATCGCTG CTGCCGCGCC GCATCCTGTA TCGCCGCTAT GAAGTCGTGG CCGGCTCGCC GGTGACGGAA AGTCATGTCG ACACGGGGAT GACGATCGCG CAGCTGTGCG CGGCGATGCT GCAGTCGGGC GACAAGGGCG CCGGCAACCT GCTGATGCGC GTGCTCGGCG GCCCGCAGGC CGTCACGTCG TTCGCGCATG AAAGCGGCGA CACCGTGTTC CGGCTCGACC GCTGGGAACC CGAACTGAAC CTGGCCGCGC CCGGCGACGA GCGCGATACG TCGACGCCGG TCGCGATGGT CGACCTGCTG CAGCAACTGC TGCTCGGCGA TACGCTGCGC GAGCCGCAGC GTGCGCAGCT CACCGAATGG ATGACCGGCG GCGCGCGCGG CGCGACCGCG ATCGCGGCCG GCGTGCCGCC CGGCTGGCGT GTCGCGGACA AGGCCGGCAC CGGCGGCTAC GGCACGACGA CCGATGTCGC GGTGCTGTGG CCGCCGTCGC GCGCGCCGAT CGTGCTGGCC GTGTCGTTCA CGCAGCCGCG TGCCGACGCC GCGGCCCGCG CGGATGTCGT CGCGTCGGCG GCGCGCATCG CGACGGGCGC GCTCACCGCA ACGGCCTGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T