BuamA.00102.a
2-dehydro-3-deoxyphosphooctonate aldolase
| CENTER ID: | BuamA.00102.a |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00102.a.A1.GE32119 | Full length( BuamA.00102.a ) | 1 | 284 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
3T4C
DEPOSITED: 7/25/2011
DETERMINATION: XRay
CLONE: BuamA.00102.a.A1.GE32119
PROTEIN: BuamA.00102.a.A1.PS01117
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|398577.6.peg.2070 |
| RefSeq: | YP_001808715.1 |
| UniProt: | B1YT10 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLYDFEVGL DQPFFLIAGT CVVESEQMTI DTAGRLKEIC AKLNVPFIYK SSYDKANRSS GKSFRGLGMD EGLRILGEVK RQLGLPVLTD VHSIDEIEQV ASVVDVLQTP AFLCRQTDFI HACARSGKPV NIKKGQFLAP HDMKNVIDKA REAAREAGLS EDRFMACERG VSFGYNNLVS DMRSLAIMRE TSAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAAVAT GVAGLFMETH PNPAEAKSDG PNAVPLNRMG ALLETLVTLD QAVKRHPFLE NDFN
NT Sequence
ATGAAGCTGT ACGATTTCGA GGTCGGTCTC GACCAGCCTT TCTTCCTGAT CGCGGGTACC TGCGTCGTCG AATCGGAGCA AATGACGATC GACACGGCGG GCCGCCTGAA GGAAATCTGC GCGAAGCTGA ACGTGCCGTT CATCTACAAG TCGTCCTATG ACAAGGCGAA CCGCAGCTCG GGCAAGTCGT TTCGCGGCCT CGGAATGGAC GAGGGCCTGC GGATCCTCGG CGAGGTGAAG CGCCAGCTCG GCCTGCCGGT GCTGACCGAC GTGCATTCGA TCGACGAGAT CGAGCAGGTC GCGTCGGTCG TCGACGTGCT GCAGACGCCC GCGTTCCTGT GCCGCCAGAC CGACTTCATC CATGCGTGCG CGCGCTCGGG CAAGCCCGTC AACATCAAGA AGGGCCAGTT CCTCGCACCG CACGACATGA AGAACGTGAT CGACAAGGCG CGCGAAGCGG CGCGTGAAGC CGGTCTGTCG GAAGACCGCT TCATGGCGTG CGAGCGCGGC GTCTCGTTCG GCTACAACAA CCTCGTGTCC GACATGCGTT CGCTCGCGAT CATGCGCGAG ACGAGCGCGC CGGTCGTGTT CGACGCGACC CACTCGGTGC AGCTGCCGGG CGGGCAGGGC ACGAGCTCGG GTGGCCAGCG CGAATTCGTG CCGGTGCTCG CGCGTGCCGC GGTCGCGACG GGCGTCGCGG GCCTGTTCAT GGAAACGCAC CCGAATCCGG CCGAAGCGAA GTCGGATGGC CCGAACGCGG TGCCGCTGAA CCGGATGGGC GCGTTGCTGG AGACCCTCGT CACGCTCGAC CAGGCGGTGA AGCGCCATCC GTTCCTGGAA AACGATTTCA AC
Details for BuamA.00102.a.A1.GE32119
| HARVESTED ON: | 4/26/2011 |
| SEQUENCED ON: | 5/2/2011 |
| EXPECTED MW: | 33kDa |
| OBSERVED MW: | 33kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 90 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
XAXHHHHHMG TLEAQTQGPG SMKLYDFEVG LDQPFFLIAG TCVVESEQMT IDTAGRLKEI CAKLNVPFIY KSSYDKANRS SGKSFRGLGM DEGLRILGEV KRQLGLPVLT DVHSIDEIEQ VASVVDVLQT PAFLCRQTDF IHACARSGKP VNIKKGQFLA PHDMKNVIDK AREAAREAGL SEDRFMACER GVSFGYNNLV SDMRSLAIMR ETSAPVVFDA THSVQLPGGQ GTSSGXQREF VXVLARAAVA XGVAGLFMET HPNXGRSEXG WPERGAAEPD GRVAGDPRHA RXXVKRHPFL ENDFXX
Validated NT Sequence
nnnnnnnnnn cnnnnnnnnn nnnngcgnnn nnnnnnnnng nnnnngnnnn nnaccntggc tnatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagctgtac gatttcgagg tcggtctcga ccagcctttc ttcctgatcg cgggtacctg cgtcgtcgaa tcggagcaaa tgacgatcga cacggcgggc cgcctgaagg aaatctgcgc gaagctgaac gtgccgttca tctacaagtc gtcctatgac aaggcgaacc gcagctcggg caagtcgttt cgcggcctcg gaatggacga gggcctgcgg atcctcggcg aggtgaagcg ccagctcggc ctgccggtgc tgaccgacgt gcattcgatc gacgagatcg agcaggtcgc gtcggtcgtc gacgtgctgc agacgcccgc gttcctgtgc cgccagaccg acttcatcca tgcgtgcgcg cgctcgggca agcccgtcaa catcaagaag ggccagttcc tcgcaccgca cgacatgaag aacgtgatcg acaaggcgcg cgaagcggcg cgtgaagccg gtctgtcgga agaccgcttc atggcgtgcg agcgcggcgt ctcgttcggc tacaacaacc tcgtgtccga catgcgttcg ctcgcgatca tgcgcgagac gagcgcgccg gtcgtgttcg acgcgaccca ctcggtgcag ctgccgggcg ggcagggcac gagctcgggt ggncagcgcg aattcgtgnc ggtgctcgcg cgtgccgcgg tcgcgacngg cgtcgcgggc ctgttcatgg aaacgcaccc gaatncnggc cgaagcgaan tcggatggcc cgaacgcggt gccgctgaac cggatgggcg cgttgctgga gaccctcgtc acgctcgacc nnnggtgaag cgccatccgt tcctggaaaa cgatttcnac nnataaacag cacgnannnn nnnnnnnnnn nancntctnn nggannnngn tgcnnannna annccgaann nnnctgnnnn nntgntggnn nnnnnnnnnn nnnnnnnnnn nn
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLYDFEVG LDQPFFLIAG TCVVESEQMT IDTAGRLKEI CAKLNVPFIY KSSYDKANRS SGKSFRGLGM DEGLRILGEV KRQLGLPVLT DVHSIDEIEQ VASVVDVLQT PAFLCRQTDF IHACARSGKP VNIKKGQFLA PHDMKNVIDK AREAAREAGL SEDRFMACER GVSFGYNNLV SDMRSLAIMR ETSAPVVFDA THSVQLPGGQ GTSSGGQREF VPVLARAAVA TGVAGLFMET HPNPAEAKSD GPNAVPLNRM GALLETLVTL DQAVKRHPFL ENDFN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagctgtac gatttcgagg tcggtctcga ccagcctttc ttcctgatcg cgggtacctg cgtcgtcgaa tcggagcaaa tgacgatcga cacggcgggc cgcctgaagg aaatctgcgc gaagctgaac gtgccgttca tctacaagtc gtcctatgac aaggcgaacc gcagctcggg caagtcgttt cgcggcctcg gaatggacga gggcctgcgg atcctcggcg aggtgaagcg ccagctcggc ctgccggtgc tgaccgacgt gcattcgatc gacgagatcg agcaggtcgc gtcggtcgtc gacgtgctgc agacgcccgc gttcctgtgc cgccagaccg acttcatcca tgcgtgcgcg cgctcgggca agcccgtcaa catcaagaag ggccagttcc tcgcaccgca cgacatgaag aacgtgatcg acaaggcgcg cgaagcggcg cgtgaagccg gtctgtcgga agaccgcttc atggcgtgcg agcgcggcgt ctcgttcggc tacaacaacc tcgtgtccga catgcgttcg ctcgcgatca tgcgcgagac gagcgcgccg gtcgtgttcg acgcgaccca ctcggtgcag ctgccgggcg ggcagggcac gagctcgggt ggccagcgcg aattcgtgcc ggtgctcgcg cgtgccgcgg tcgcgacggg cgtcgcgggc ctgttcatgg aaacgcaccc gaatccggcc gaagcgaagt cggatggccc gaacgcggtg ccgctgaacc ggatgggcgc gttgctggag accctcgtca cgctcgacca ggcggtgaag cgccatccgt tcctggaaaa cgatttcaac taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa