SSGCID: Target Page

BuamA.00098.b

Molybdopterin biosynthesis MoaE protein

CENTER ID:	BuamA.00098.b
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00098.b.B1.GE39778	Full length( BuamA.00098.b )		1	187

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.6923
RefSeq:	YP_001816428.1
UniProt:	B1Z562

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTSFTESRGA PPDDPSRDGS RPEPSALHTE PPPAIAAGFE VRVQYAAIDA GAEIAPIVRN PNVGAVVNFL GVVRNEGDVD DVIALDVEHY PTMTEQALWS IVEEAGARWQ LEAVRIVHRV GRIPLGQPVV IVVVAAAHRA SAFDACEFIM DFVKTHAPFW KKEIRHDGVS GWVEPKAHDD QAMRRWG

NT Sequence

ATGACGAGTT TCACCGAATC CCGTGGCGCG CCACCCGACG ATCCGTCGCG TGACGGCAGC CGTCCCGAGC CATCCGCGCT GCACACCGAA CCGCCGCCGG CGATTGCCGC CGGCTTCGAG GTGCGCGTGC AATACGCGGC GATCGACGCC GGCGCCGAGA TCGCGCCGAT CGTTCGCAAT CCGAATGTCG GCGCGGTCGT GAATTTCCTC GGCGTCGTGC GCAACGAAGG CGACGTCGAC GACGTGATCG CGCTCGACGT CGAGCACTAT CCGACGATGA CCGAGCAGGC GCTTTGGAGC ATCGTCGAGG AAGCCGGCGC GCGCTGGCAG CTCGAAGCGG TCAGGATCGT GCATCGCGTC GGGCGGATTC CGCTCGGCCA GCCGGTCGTG ATCGTCGTGG TCGCCGCCGC GCATCGCGCA TCGGCGTTCG ATGCGTGCGA GTTCATCATG GACTTCGTGA AGACGCACGC GCCGTTCTGG AAGAAGGAGA TCCGGCACGA CGGCGTGTCC GGCTGGGTCG AGCCGAAGGC ACATGACGAC CAGGCGATGC GGCGATGGGG A

Details for BuamA.00098.b.B1.GE39778

HARVESTED ON:	2/4/2016
SEQUENCED ON:	1/27/2016
EXPECTED MW:	21kDa
OBSERVED MW:	21kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT SFTESRGAPP DDPSRDGSRP EPSALHTEPP PAIAAGFEVR VQYAAIDAGA EIAPIVRNPN VGAVVNFLGV VRNEGDVDDV IALDVEHYPT MTEQALWSIV EEAGARWQLE AVRIVHRVGR IPLGQPVVIV VVAAAHRASA FDACEFIMDF VKTHAPFWKK EIRHDGVSGW VEPKAHDDQA MRRWG

Validated NT Sequence

atcttactca cttatcccca tcgccgcatc gcctggtcgt catgtgcctt cggctcgacc cagccggaca cgccgtcgtg ccggatctcc ttcttccaga acggcgcgtg cgtcttcacg aagtccatga tgaactcgca cgcatcgaac gccgatgcgc gatgcgcggc ggcgaccacg acgatcacga ccggctggcc gagcggaatc cgcccgacgc gatgcacgat cctgaccgct tcgagctgcc agcgcgcgcc ggcttcctcg acgatgctcc aaagcgcctg ctcggtcatc gtcggatagt gctcgacgtc gagcgcgatc acgtcgtcga cgtcgccttc gttgcgcacg acgccgagga aattcacgac cgcgccgaca ttcggattgc gaacgatcgg cgcgatctcg gcgccggcgt cgatcgccgc gtattgcacg cgcacctcga agccggcggc aatcgccggc ggcggttcgg tgtgcagcgc ggatggctcg ggacggctgc cgtcacgcga cggatcgtcg ggtggcgcgc cacgggattc ggtgaaactc gtcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga gtttcaccga atcccgtggc gcgccacccg acgatccgtc gcgtgacggc agccgtcccg agccatccgc gctgcacacc gaaccgccgc cggcgattgc cgccggcttc gaggtgcgcg tgcaatacgc ggcgatcgac gccggcgccg agatcgcgcc gatcgttcgc aatccgaatg tcggcgcggt cgtgaatttc ctcggcgtcg tgcgcaacga aggcgacgtc gacgacgtga tcgcgctcga cgtcgagcac tatccgacga tgaccgagca ggcgctttgg agcatcgtcg aggaagccgg cgcgcgctgg cagctcgaag cggtcaggat cgtgcatcgc gtcgggcgga ttccgctcgg ccagccggtc gtgatcgtcg tggtcgccgc cgcgcatcgc gcatcggcgt tcgatgcgtg cgagttcatc atggacttcg tgaagacgca cgcgccgttc tggaagaagg agatccggca cgacggcgtg tccggctggg tcgagccgaa ggcacatgac gaccaggcga tgcggcgatg gggatgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat