BuamA.00096.a

8-amino-7-oxononanoate synthase

CENTER ID: BuamA.00096.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00096.a.B1.GE39776 Full length( BuamA.00096.a ) 1 445
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00096.a.B1.PS37921 Full length( BuamA.00096.a ) 1 445

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2937
RefSeq: YP_001809536.1
UniProt: B1YNS0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTRTAPSSLR SLPAEGAAAT DLLDTLQRGL AELDAQGLRR VRRTADTACD AHMRVDGRDI VGFASNDYLG LAAHPALVAA FAEGARRYGS GSGGSHLLGG HSRAHATLED ELAGFAGGFS DAPRALYFST GYMANLAAMT ALTGKHATIF SDALNHASLI DGIRLSRANV QVYPHADMAA LAALLDASDA ETKLIVSDTV FSMDGDIAPL AELVALAERH GAWLVVDDAH GFGVLGPQGR GALAAAALRS PNLIYVGTLG KAAGVAGAFV IAHETVIEWM IQRARSYIFT TAAPPAVAHA VSASLKVIAG DEGDARRAHL AALIERTRAL LRMTRWQPVD SHTAVQPLVI GSNDATLAAM RSLDAHGLWV PAIRPPTVPA GTSRLRVSLS AAHSFDDLAR LEAALIEASE GQTRRDAEQP PRSLRSLPPE GAAASLGAAR RETAA
NT Sequence
ATGACACGAA CGGCCCCCAG CTCACTTCGC TCGCTGCCCG CCGAGGGGGC GGCAGCAACG GATCTGCTCG ATACGCTGCA GCGCGGCCTA GCCGAGCTCG ACGCGCAAGG GTTGCGCCGC GTGCGCCGCA CCGCCGACAC CGCGTGCGAC GCGCACATGC GCGTCGACGG CCGCGACATC GTCGGCTTCG CGAGTAACGA CTATCTCGGC CTCGCCGCGC ATCCGGCGCT CGTCGCCGCG TTCGCCGAAG GCGCGCGGCG CTACGGCTCC GGCAGCGGCG GCTCGCATCT GCTCGGCGGC CATTCGCGTG CGCACGCCAC GCTCGAGGAC GAACTCGCCG GCTTCGCGGG CGGCTTCTCC GATGCGCCGC GCGCGCTGTA CTTCAGCACC GGCTACATGG CGAACCTCGC CGCGATGACG GCGCTCACCG GCAAACACGC GACGATCTTC TCCGATGCGC TGAACCACGC GTCGCTGATC GACGGGATCC GGCTGTCGCG CGCGAACGTG CAGGTCTACC CGCATGCGGA CATGGCCGCG CTCGCCGCGC TGCTCGACGC GTCCGACGCC GAAACGAAAC TGATCGTCAG CGACACCGTG TTCAGCATGG ATGGCGACAT CGCGCCGCTC GCCGAACTCG TCGCGCTGGC CGAACGCCAC GGCGCGTGGC TCGTCGTCGA CGACGCGCAC GGTTTCGGCG TGCTGGGGCC CCAAGGCCGC GGCGCGCTCG CGGCCGCCGC GCTGCGCTCG CCGAACCTGA TCTACGTCGG CACGCTCGGC AAGGCCGCCG GCGTCGCGGG CGCGTTCGTG ATCGCGCACG AGACCGTGAT CGAATGGATG ATCCAGCGCG CGCGCAGCTA CATCTTCACG ACCGCCGCGC CGCCGGCCGT CGCGCATGCG GTATCGGCGA GCCTGAAGGT GATCGCCGGC GACGAAGGCG ACGCGCGGCG CGCGCACCTC GCCGCGCTGA TCGAGCGCAC GCGCGCGCTG CTGCGCATGA CGCGCTGGCA GCCGGTCGAC TCGCACACGG CGGTGCAGCC GCTCGTGATC GGCAGCAACG ACGCGACGCT TGCGGCGATG CGCTCGCTCG ACGCGCACGG CCTGTGGGTG CCCGCGATCC GGCCGCCGAC GGTGCCCGCC GGCACGTCGC GGCTGCGCGT ATCGCTGTCG GCAGCACATT CGTTCGACGA TCTCGCACGG CTCGAAGCAG CATTGATCGA AGCCAGCGAG GGGCAGACCC GGCGGGATGC AGAACAGCCC CCACGCTCAC TGCGTTCGCT GCCCCCCGAG GGTGCTGCAG CCTCCCTTGG GGCGGCCCGG CGGGAGACTG CGGCA
Details for BuamA.00096.a.B1.GE39776
HARVESTED ON: 2/4/2016
SEQUENCED ON: 1/27/2016
EXPECTED MW: 47kDa
OBSERVED MW: 47kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Validated AA Sequence
MAHHHHHHMT RTAPSSLRSL PAEGAAATDL LDTLQRGLAE LDAQGLRRVR RTADTACDAH MRVDGRDIVG FASNDYLGLA AHPALVAAFA EGARRYGSGS GGSHLLGGHS RAHATLEDEL AGFAGGFSDA PRALYFSTGY MANLAAMTAL TGKHATIFSD ALNHASLIDG IRLSRANVQV YPHADMAALA ALLDASDAET KLIVSDTVFS MDGDIAPLAE LVALAERHGA WLVVDDAHGF GVLGPQGRGA LAAAALRSPN LIYVGTLGKA AGVAGAFVIA HETVIEWMIQ RARSYIFTTA APPAVAHAVS ASLKVIAGDE GDARRAHLAA LIERTRALLR MTRWQPVDSH TAVQPLVIGS NDATLAAMRS LDAHGLWVPA IRPPTVPAGT SRLRVSLSAA HSFDDLARLE AALIEASEGQ TRRDAEQPPR SXRSLPPEGA AA
Validated NT Sequence
ggaggctgca gcaccctcgg ggggcagcga acgnnntgag cgtgggggct gttctgcatc ccgccgggtc tgcccctcgc tggcttcgat caatgctgct tcgagccgtg cgagatcgtc gaacgaatgt gctgccgaca gcgatacgcg cagccgcgac gtgccggcgg gcaccgtcgg cggccggatc gcgggcaccc acaggccgtg cgcgtcgagc gagcgcatcg ccgcaagcgt cgcgtcgttg ctgccgatca cgagcggctg caccgccgtg tgcgagtcga ccggctgcca gcgcgtcatg cgcagcagcg cgcgcgtgcg ctcgatcagc gcggcgaggt gcgcgcgccg cgcgtcgcct tcgtcgccgg cgatcacctt caggctcgcc gataccgcat gcgcgacggc cggcggcgcg gcggtcgtga agatgtagct gcgcgcgcgc tggatcatcc attcgatcac ggtctcgtgc gcgatcacga acgcgcccgc gacgccggcg gccttgccga gcgtgccgac gtagatcagg ttcggcgagc gcagcgcggc ggccgcgagc gcgccgcggc cttggggccc cagcacgccg aaaccgtgcg cgtcgtcgac gacgagccac gcgccgtggc gttcggccag cgcgacgagt tcggcgagcg gcgcgatgtc gccatccatg ctgaacacgg tgtcgctgac gatcagtttc gtttcggcgt cggacgcgtc gagcagcgcg gcgagcgcgg ccatgtccgc atgcgggtag acctgcacgt tcgcgcgcga cagccggatc ccgtcgatca gcgacgcgtg gttcagcgca tcggagaaga tcgtcgcgtg tttgccggtg agcgccgtca tcgcggcgag gttcgccatg tagccggtgc tgaagtacag cgcgcgcggc gcatcggaga agccgcccgc gaagccggcg agttcgtcct cgagcgtggc gtgcgcacgc gaatggccgc cgagcagatg cgagccgccg ctgccggagc cgtagcgccg cgcgccttcg gcgaacgcgg cgacgagcgc cggatgcgcg gcgaggccga gatagtcgtt actcgcgaag ccgacgatgt cgcggccgtc gacgcgcatg tgcgcgtcgc acgcggtgtc ggcggtgcgg cgcacgcggc gcaacccttg cgcgtcgagc tcggctaggc cgcgctgcag cgtatcgagc agatccgttg ctgccgcccc ctcggcgggc agcgagcgaa gtgagctggg ggccgttcgt gtcatatggt ggtggtggtg gtgagccatg g
Expected Protein Sequence
MAHHHHHHMT RTAPSSLRSL PAEGAAATDL LDTLQRGLAE LDAQGLRRVR RTADTACDAH MRVDGRDIVG FASNDYLGLA AHPALVAAFA EGARRYGSGS GGSHLLGGHS RAHATLEDEL AGFAGGFSDA PRALYFSTGY MANLAAMTAL TGKHATIFSD ALNHASLIDG IRLSRANVQV YPHADMAALA ALLDASDAET KLIVSDTVFS MDGDIAPLAE LVALAERHGA WLVVDDAHGF GVLGPQGRGA LAAAALRSPN LIYVGTLGKA AGVAGAFVIA HETVIEWMIQ RARSYIFTTA APPAVAHAVS ASLKVIAGDE GDARRAHLAA LIERTRALLR MTRWQPVDSH TAVQPLVIGS NDATLAAMRS LDAHGLWVPA IRPPTVPAGT SRLRVSLSAA HSFDDLARLE AALIEASEGQ TRRDAEQPPR SLRSLPPEGA AASLGAARRE TAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacac gaacggcccc cagctcactt cgctcgctgc ccgccgaggg ggcggcagca acggatctgc tcgatacgct gcagcgcggc ctagccgagc tcgacgcgca agggttgcgc cgcgtgcgcc gcaccgccga caccgcgtgc gacgcgcaca tgcgcgtcga cggccgcgac atcgtcggct tcgcgagtaa cgactatctc ggcctcgccg cgcatccggc gctcgtcgcc gcgttcgccg aaggcgcgcg gcgctacggc tccggcagcg gcggctcgca tctgctcggc ggccattcgc gtgcgcacgc cacgctcgag gacgaactcg ccggcttcgc gggcggcttc tccgatgcgc cgcgcgcgct gtacttcagc accggctaca tggcgaacct cgccgcgatg acggcgctca ccggcaaaca cgcgacgatc ttctccgatg cgctgaacca cgcgtcgctg atcgacggga tccggctgtc gcgcgcgaac gtgcaggtct acccgcatgc ggacatggcc gcgctcgccg cgctgctcga cgcgtccgac gccgaaacga aactgatcgt cagcgacacc gtgttcagca tggatggcga catcgcgccg ctcgccgaac tcgtcgcgct ggccgaacgc cacggcgcgt ggctcgtcgt cgacgacgcg cacggtttcg gcgtgctggg gccccaaggc cgcggcgcgc tcgcggccgc cgcgctgcgc tcgccgaacc tgatctacgt cggcacgctc ggcaaggccg ccggcgtcgc gggcgcgttc gtgatcgcgc acgagaccgt gatcgaatgg atgatccagc gcgcgcgcag ctacatcttc acgaccgccg cgccgccggc cgtcgcgcat gcggtatcgg cgagcctgaa ggtgatcgcc ggcgacgaag gcgacgcgcg gcgcgcgcac ctcgccgcgc tgatcgagcg cacgcgcgcg ctgctgcgca tgacgcgctg gcagccggtc gactcgcaca cggcggtgca gccgctcgtg atcggcagca acgacgcgac gcttgcggcg atgcgctcgc tcgacgcgca cggcctgtgg gtgcccgcga tccggccgcc gacggtgccc gccggcacgt cgcggctgcg cgtatcgctg tcggcagcac attcgttcga cgatctcgca cggctcgaag cagcattgat cgaagccagc gaggggcaga cccggcggga tgcagaacag cccccacgct cactgcgttc gctgcccccc gagggtgctg cagcctccct tggggcggcc cggcgggaga ctgcggcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuamA.00096.a.B1.PS37921
PURIFICATION DATe: 4/26/2016
CONCENTRATION: 20.6mg/ml
OBSERVED MW: 46kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 18
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 97
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMT RTAPSSLRSL PAEGAAATDL LDTLQRGLAE LDAQGLRRVR RTADTACDAH MRVDGRDIVG FASNDYLGLA AHPALVAAFA EGARRYGSGS GGSHLLGGHS RAHATLEDEL AGFAGGFSDA PRALYFSTGY MANLAAMTAL TGKHATIFSD ALNHASLIDG IRLSRANVQV YPHADMAALA ALLDASDAET KLIVSDTVFS MDGDIAPLAE LVALAERHGA WLVVDDAHGF GVLGPQGRGA LAAAALRSPN LIYVGTLGKA AGVAGAFVIA HETVIEWMIQ RARSYIFTTA APPAVAHAVS ASLKVIAGDE GDARRAHLAA LIERTRALLR MTRWQPVDSH TAVQPLVIGS NDATLAAMRS LDAHGLWVPA IRPPTVPAGT SRLRVSLSAA HSFDDLARLE AALIEASEGQ TRRDAEQPPR SXRSLPPEGA AA
Validated NT Sequence
ggaggctgca gcaccctcgg ggggcagcga acgnnntgag cgtgggggct gttctgcatc ccgccgggtc tgcccctcgc tggcttcgat caatgctgct tcgagccgtg cgagatcgtc gaacgaatgt gctgccgaca gcgatacgcg cagccgcgac gtgccggcgg gcaccgtcgg cggccggatc gcgggcaccc acaggccgtg cgcgtcgagc gagcgcatcg ccgcaagcgt cgcgtcgttg ctgccgatca cgagcggctg caccgccgtg tgcgagtcga ccggctgcca gcgcgtcatg cgcagcagcg cgcgcgtgcg ctcgatcagc gcggcgaggt gcgcgcgccg cgcgtcgcct tcgtcgccgg cgatcacctt caggctcgcc gataccgcat gcgcgacggc cggcggcgcg gcggtcgtga agatgtagct gcgcgcgcgc tggatcatcc attcgatcac ggtctcgtgc gcgatcacga acgcgcccgc gacgccggcg gccttgccga gcgtgccgac gtagatcagg ttcggcgagc gcagcgcggc ggccgcgagc gcgccgcggc cttggggccc cagcacgccg aaaccgtgcg cgtcgtcgac gacgagccac gcgccgtggc gttcggccag cgcgacgagt tcggcgagcg gcgcgatgtc gccatccatg ctgaacacgg tgtcgctgac gatcagtttc gtttcggcgt cggacgcgtc gagcagcgcg gcgagcgcgg ccatgtccgc atgcgggtag acctgcacgt tcgcgcgcga cagccggatc ccgtcgatca gcgacgcgtg gttcagcgca tcggagaaga tcgtcgcgtg tttgccggtg agcgccgtca tcgcggcgag gttcgccatg tagccggtgc tgaagtacag cgcgcgcggc gcatcggaga agccgcccgc gaagccggcg agttcgtcct cgagcgtggc gtgcgcacgc gaatggccgc cgagcagatg cgagccgccg ctgccggagc cgtagcgccg cgcgccttcg gcgaacgcgg cgacgagcgc cggatgcgcg gcgaggccga gatagtcgtt actcgcgaag ccgacgatgt cgcggccgtc gacgcgcatg tgcgcgtcgc acgcggtgtc ggcggtgcgg cgcacgcggc gcaacccttg cgcgtcgagc tcggctaggc cgcgctgcag cgtatcgagc agatccgttg ctgccgcccc ctcggcgggc agcgagcgaa gtgagctggg ggccgttcgt gtcatatggt ggtggtggtg gtgagccatg g
Expressed Protein Sequence
MAHHHHHHMT RTAPSSLRSL PAEGAAATDL LDTLQRGLAE LDAQGLRRVR RTADTACDAH MRVDGRDIVG FASNDYLGLA AHPALVAAFA EGARRYGSGS GGSHLLGGHS RAHATLEDEL AGFAGGFSDA PRALYFSTGY MANLAAMTAL TGKHATIFSD ALNHASLIDG IRLSRANVQV YPHADMAALA ALLDASDAET KLIVSDTVFS MDGDIAPLAE LVALAERHGA WLVVDDAHGF GVLGPQGRGA LAAAALRSPN LIYVGTLGKA AGVAGAFVIA HETVIEWMIQ RARSYIFTTA APPAVAHAVS ASLKVIAGDE GDARRAHLAA LIERTRALLR MTRWQPVDSH TAVQPLVIGS NDATLAAMRS LDAHGLWVPA IRPPTVPAGT SRLRVSLSAA HSFDDLARLE AALIEASEGQ TRRDAEQPPR SLRSLPPEGA AASLGAARRE TAA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACAC GAACGGCCCC CAGCTCACTT CGCTCGCTGC CCGCCGAGGG GGCGGCAGCA ACGGATCTGC TCGATACGCT GCAGCGCGGC CTAGCCGAGC TCGACGCGCA AGGGTTGCGC CGCGTGCGCC GCACCGCCGA CACCGCGTGC GACGCGCACA TGCGCGTCGA CGGCCGCGAC ATCGTCGGCT TCGCGAGTAA CGACTATCTC GGCCTCGCCG CGCATCCGGC GCTCGTCGCC GCGTTCGCCG AAGGCGCGCG GCGCTACGGC TCCGGCAGCG GCGGCTCGCA TCTGCTCGGC GGCCATTCGC GTGCGCACGC CACGCTCGAG GACGAACTCG CCGGCTTCGC GGGCGGCTTC TCCGATGCGC CGCGCGCGCT GTACTTCAGC ACCGGCTACA TGGCGAACCT CGCCGCGATG ACGGCGCTCA CCGGCAAACA CGCGACGATC TTCTCCGATG CGCTGAACCA CGCGTCGCTG ATCGACGGGA TCCGGCTGTC GCGCGCGAAC GTGCAGGTCT ACCCGCATGC GGACATGGCC GCGCTCGCCG CGCTGCTCGA CGCGTCCGAC GCCGAAACGA AACTGATCGT CAGCGACACC GTGTTCAGCA TGGATGGCGA CATCGCGCCG CTCGCCGAAC TCGTCGCGCT GGCCGAACGC CACGGCGCGT GGCTCGTCGT CGACGACGCG CACGGTTTCG GCGTGCTGGG GCCCCAAGGC CGCGGCGCGC TCGCGGCCGC CGCGCTGCGC TCGCCGAACC TGATCTACGT CGGCACGCTC GGCAAGGCCG CCGGCGTCGC GGGCGCGTTC GTGATCGCGC ACGAGACCGT GATCGAATGG ATGATCCAGC GCGCGCGCAG CTACATCTTC ACGACCGCCG CGCCGCCGGC CGTCGCGCAT GCGGTATCGG CGAGCCTGAA GGTGATCGCC GGCGACGAAG GCGACGCGCG GCGCGCGCAC CTCGCCGCGC TGATCGAGCG CACGCGCGCG CTGCTGCGCA TGACGCGCTG GCAGCCGGTC GACTCGCACA CGGCGGTGCA GCCGCTCGTG ATCGGCAGCA ACGACGCGAC GCTTGCGGCG ATGCGCTCGC TCGACGCGCA CGGCCTGTGG GTGCCCGCGA TCCGGCCGCC GACGGTGCCC GCCGGCACGT CGCGGCTGCG CGTATCGCTG TCGGCAGCAC ATTCGTTCGA CGATCTCGCA CGGCTCGAAG CAGCATTGAT CGAAGCCAGC GAGGGGCAGA CCCGGCGGGA TGCAGAACAG CCCCCACGCT CACTGCGTTC GCTGCCCCCC GAGGGTGCTG CAGCCTCCCT TGGGGCGGCC CGGCGGGAGA CTGCGGCATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT