BuamA.00095.d

Alpha/beta hydrolase fold

CENTER ID: BuamA.00095.d
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00095.d.B1.GE39928 Full length( BuamA.00095.d ) 1 270
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00095.d.B1.PS37941 Full length( BuamA.00095.d ) 1 270

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.6324
RefSeq: YP_001815854.1
UniProt: B1Z3I8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAEFADDDL VRFDAQGGTP LPAADAEGWL DHDGARIWHA SFGDGPPVVL LHGGLGHSGN WGNQVPALLA AGYRAIVIDS RGHGRSTRDD RPYSYERMAS DVLAVLDALH IGRARFVGWS DGACVSLVLA ARAPQRAAGV FFFACNMDPG GTKEMVPSPL IDRCFARHRK DYARLSATPD QFDAFVAAVS EMMRTQPDYS AADLAAIAVS VAIVQGEHDE FIRPEHAAYL ARTIPGATLT ILPGVSHFAP LQRPARFNAA MLAFLDRLPD
NT Sequence
ATGAATGCGG AATTCGCCGA CGACGACCTC GTCCGCTTCG ACGCGCAAGG CGGCACGCCG CTGCCCGCGG CCGATGCCGA AGGATGGCTT GACCACGACG GCGCGCGCAT CTGGCACGCG TCGTTCGGCG ACGGCCCGCC AGTCGTGCTG CTGCACGGTG GCCTCGGGCA CAGCGGCAAC TGGGGTAACC AGGTGCCGGC GCTGCTCGCG GCCGGCTATC GCGCGATCGT GATCGACAGC CGCGGCCACG GCCGCAGCAC GCGCGACGAT CGCCCCTATT CGTACGAACG CATGGCCTCC GACGTGCTCG CGGTACTCGA TGCGCTGCAC ATCGGGCGCG CGCGCTTCGT CGGCTGGAGC GACGGCGCGT GCGTGTCGCT CGTGCTGGCC GCCCGCGCGC CGCAGCGCGC GGCGGGCGTG TTCTTCTTCG CGTGCAACAT GGATCCGGGC GGCACGAAGG AGATGGTGCC GAGCCCGCTG ATCGACCGCT GCTTCGCGCG GCACCGCAAG GACTACGCGC GACTGTCCGC GACGCCCGAC CAGTTCGACG CGTTCGTCGC GGCCGTCAGC GAAATGATGC GCACGCAACC GGATTACAGC GCGGCCGACC TCGCTGCGAT CGCGGTGTCG GTCGCGATCG TGCAGGGCGA GCACGACGAG TTCATCCGCC CCGAGCATGC GGCCTATCTC GCGCGCACGA TTCCGGGCGC GACGCTGACG ATCCTGCCCG GCGTGAGCCA CTTCGCGCCG CTGCAGCGGC CGGCGCGTTT CAACGCCGCG ATGCTGGCCT TTCTCGACCG GTTGCCTGAC
Details for BuamA.00095.d.B1.GE39928
HARVESTED ON: 2/26/2016
SEQUENCED ON: 3/1/2016
EXPECTED MW: 30kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMN AEFADDDLVR FDAQGGTPLP AADAEGWLDH DGARIWHASF GDGPPVVLLH GGLGHSGNWG NQVPALLAAG YRAIVIDSRG HGRSTRDDRP YSYERMASDV LAVLDALHIG RARFVGWSDG ACVSLVLAAR APQRAAGVFF FACNMDPGGT KEMVPSPLID RCFARHRKDY ARLSATPDQF DAFVAAVSEM MRTQPDYSAA DLAAIAVSVA IVQGEHDEFI RPEHAAYLAR TIPGATLTIL PGVSHFAPLQ RPARFNAXXX XXLDRLPD
Validated NT Sequence
atcttactca cttagtcagg caaccggtcg agnnnnnccn nnnnnnnggc gttgaaacgc gccggccgct gcagcggcgc gaagtggctc acgccgggca ggatcgtcag cgtcgcgccc ggaatcgtgc gcgcgagata ggccgcatgc tcggggcgga tgaactcgtc gtgctcgccc tgcacgatcg cgaccgacac cgcgatcgca gcgaggtcgg ccgcgctgta atccggttgc gtgcgcatca tttcgctgac ggccgcgacg aacgcgtcga actggtcggg cgtcgcggac agtcgcgcgt agtccttgcg gtgccgcgcg aagcagcggt cgatcagcgg gctcggcacc atctccttcg tgccgcccgg atccatgttg cacgcgaaga agaacacgcc cgccgcgcgc tgcggcgcgc gggcggccag cacgagcgac acgcacgcgc cgtcgctcca gccgacgaag cgcgcgcgcc cgatgtgcag cgcatcgagt accgcgagca cgtcggaggc catgcgttcg tacgaatagg ggcgatcgtc gcgcgtgctg cggccgtggc cgcggctgtc gatcacgatc gcgcgatagc cggccgcgag cagcgccggc acctggttac cccagttgcc gctgtgcccg aggccaccgt gcagcagcac gactggcggg ccgtcgccga acgacgcgtg ccagatgcgc gcgccgtcgt ggtcaagcca tccttcggca tcggccgcgg gcagcggcgt gccgccttgc gcgtcgaagc ggacgaggtc gtcgtcggcg aattccgcat tcatatggtg gtggtggtgg tgagccatgg tanatc
Expected Protein Sequence
MAHHHHHHMN AEFADDDLVR FDAQGGTPLP AADAEGWLDH DGARIWHASF GDGPPVVLLH GGLGHSGNWG NQVPALLAAG YRAIVIDSRG HGRSTRDDRP YSYERMASDV LAVLDALHIG RARFVGWSDG ACVSLVLAAR APQRAAGVFF FACNMDPGGT KEMVPSPLID RCFARHRKDY ARLSATPDQF DAFVAAVSEM MRTQPDYSAA DLAAIAVSVA IVQGEHDEFI RPEHAAYLAR TIPGATLTIL PGVSHFAPLQ RPARFNAAML AFLDRLPD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg cggaattcgc cgacgacgac ctcgtccgct tcgacgcgca aggcggcacg ccgctgcccg cggccgatgc cgaaggatgg cttgaccacg acggcgcgcg catctggcac gcgtcgttcg gcgacggccc gccagtcgtg ctgctgcacg gtggcctcgg gcacagcggc aactggggta accaggtgcc ggcgctgctc gcggccggct atcgcgcgat cgtgatcgac agccgcggcc acggccgcag cacgcgcgac gatcgcccct attcgtacga acgcatggcc tccgacgtgc tcgcggtact cgatgcgctg cacatcgggc gcgcgcgctt cgtcggctgg agcgacggcg cgtgcgtgtc gctcgtgctg gccgcccgcg cgccgcagcg cgcggcgggc gtgttcttct tcgcgtgcaa catggatccg ggcggcacga aggagatggt gccgagcccg ctgatcgacc gctgcttcgc gcggcaccgc aaggactacg cgcgactgtc cgcgacgccc gaccagttcg acgcgttcgt cgcggccgtc agcgaaatga tgcgcacgca accggattac agcgcggccg acctcgctgc gatcgcggtg tcggtcgcga tcgtgcaggg cgagcacgac gagttcatcc gccccgagca tgcggcctat ctcgcgcgca cgattccggg cgcgacgctg acgatcctgc ccggcgtgag ccacttcgcg ccgctgcagc ggccggcgcg tttcaacgcc gcgatgctgg cctttctcga ccggttgcct gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BuamA.00095.d.B1.PS37941
PURIFICATION DATe: 6/7/2016
CONCENTRATION: 36.7mg/ml
OBSERVED MW: 32kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 11
VIAL VOLUME: 200µl
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMN AEFADDDLVR FDAQGGTPLP AADAEGWLDH DGARIWHASF GDGPPVVLLH GGLGHSGNWG NQVPALLAAG YRAIVIDSRG HGRSTRDDRP YSYERMASDV LAVLDALHIG RARFVGWSDG ACVSLVLAAR APQRAAGVFF FACNMDPGGT KEMVPSPLID RCFARHRKDY ARLSATPDQF DAFVAAVSEM MRTQPDYSAA DLAAIAVSVA IVQGEHDEFI RPEHAAYLAR TIPGATLTIL PGVSHFAPLQ RPARFNAXXX XXLDRLPD
Validated NT Sequence
atcttactca cttagtcagg caaccggtcg agnnnnnccn nnnnnnnggc gttgaaacgc gccggccgct gcagcggcgc gaagtggctc acgccgggca ggatcgtcag cgtcgcgccc ggaatcgtgc gcgcgagata ggccgcatgc tcggggcgga tgaactcgtc gtgctcgccc tgcacgatcg cgaccgacac cgcgatcgca gcgaggtcgg ccgcgctgta atccggttgc gtgcgcatca tttcgctgac ggccgcgacg aacgcgtcga actggtcggg cgtcgcggac agtcgcgcgt agtccttgcg gtgccgcgcg aagcagcggt cgatcagcgg gctcggcacc atctccttcg tgccgcccgg atccatgttg cacgcgaaga agaacacgcc cgccgcgcgc tgcggcgcgc gggcggccag cacgagcgac acgcacgcgc cgtcgctcca gccgacgaag cgcgcgcgcc cgatgtgcag cgcatcgagt accgcgagca cgtcggaggc catgcgttcg tacgaatagg ggcgatcgtc gcgcgtgctg cggccgtggc cgcggctgtc gatcacgatc gcgcgatagc cggccgcgag cagcgccggc acctggttac cccagttgcc gctgtgcccg aggccaccgt gcagcagcac gactggcggg ccgtcgccga acgacgcgtg ccagatgcgc gcgccgtcgt ggtcaagcca tccttcggca tcggccgcgg gcagcggcgt gccgccttgc gcgtcgaagc ggacgaggtc gtcgtcggcg aattccgcat tcatatggtg gtggtggtgg tgagccatgg tanatc
Expressed Protein Sequence
MAHHHHHHMN AEFADDDLVR FDAQGGTPLP AADAEGWLDH DGARIWHASF GDGPPVVLLH GGLGHSGNWG NQVPALLAAG YRAIVIDSRG HGRSTRDDRP YSYERMASDV LAVLDALHIG RARFVGWSDG ACVSLVLAAR APQRAAGVFF FACNMDPGGT KEMVPSPLID RCFARHRKDY ARLSATPDQF DAFVAAVSEM MRTQPDYSAA DLAAIAVSVA IVQGEHDEFI RPEHAAYLAR TIPGATLTIL PGVSHFAPLQ RPARFNAAML AFLDRLPD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG CGGAATTCGC CGACGACGAC CTCGTCCGCT TCGACGCGCA AGGCGGCACG CCGCTGCCCG CGGCCGATGC CGAAGGATGG CTTGACCACG ACGGCGCGCG CATCTGGCAC GCGTCGTTCG GCGACGGCCC GCCAGTCGTG CTGCTGCACG GTGGCCTCGG GCACAGCGGC AACTGGGGTA ACCAGGTGCC GGCGCTGCTC GCGGCCGGCT ATCGCGCGAT CGTGATCGAC AGCCGCGGCC ACGGCCGCAG CACGCGCGAC GATCGCCCCT ATTCGTACGA ACGCATGGCC TCCGACGTGC TCGCGGTACT CGATGCGCTG CACATCGGGC GCGCGCGCTT CGTCGGCTGG AGCGACGGCG CGTGCGTGTC GCTCGTGCTG GCCGCCCGCG CGCCGCAGCG CGCGGCGGGC GTGTTCTTCT TCGCGTGCAA CATGGATCCG GGCGGCACGA AGGAGATGGT GCCGAGCCCG CTGATCGACC GCTGCTTCGC GCGGCACCGC AAGGACTACG CGCGACTGTC CGCGACGCCC GACCAGTTCG ACGCGTTCGT CGCGGCCGTC AGCGAAATGA TGCGCACGCA ACCGGATTAC AGCGCGGCCG ACCTCGCTGC GATCGCGGTG TCGGTCGCGA TCGTGCAGGG CGAGCACGAC GAGTTCATCC GCCCCGAGCA TGCGGCCTAT CTCGCGCGCA CGATTCCGGG CGCGACGCTG ACGATCCTGC CCGGCGTGAG CCACTTCGCG CCGCTGCAGC GGCCGGCGCG TTTCAACGCC GCGATGCTGG CCTTTCTCGA CCGGTTGCCT GACTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT