BuamA.00095.a
Alpha/beta hydrolase fold protein
| CENTER ID: | BuamA.00095.a |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00095.a.B1.GE39927 | Full length( BuamA.00095.a ) | 1 | 267 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00095.a.B1.PS38005 | Full length( BuamA.00095.a ) | 1 | 267 |
Structures
5W15
DEPOSITED: 6/1/2017
DETERMINATION: XRay
CLONE: BuamA.00095.a.B1.GE39927
PROTEIN: BuamA.00095.a.B1.PS38005
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|398577.6.peg.3184 |
| RefSeq: | YP_001809767.1 |
| UniProt: | B1YPY7 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDLANRFNF EGHRIAWGTL GEGPPLVLVH GTPFSSQVWR RIAPWLARRH RVFFYDLLGY GQSDMPDADV SLGRQNVLFG ALLDEWKISR PRVLAHDYGG ATVLRAHFLD GIAYSDLTLV NPVAIAPQGS PFVRHVAQHE AAFTGLPAYA HHALVSAYIG QAVAQPLSDD VLSIYRAPWL TPAGQAAFYR QIAQMRQRYI EDAEARYAPP DFPVRIVWGE DDRWIPLEQG QALADRIANG KLIRVPRAGH LVQEDAPEAI VAAVLDR
NT Sequence
ATGCTCGATC TCGCCAATCG CTTCAATTTC GAAGGTCACC GGATCGCTTG GGGCACGCTC GGCGAAGGGC CGCCGCTCGT GCTTGTGCAC GGCACGCCGT TTTCATCGCA GGTGTGGCGC CGGATCGCGC CGTGGCTCGC GCGGCGCCAT CGCGTGTTCT TCTACGACCT GCTCGGCTAC GGCCAGTCCG ACATGCCCGA CGCGGACGTA TCGCTCGGCC GGCAGAACGT GTTGTTCGGC GCGCTGCTCG ACGAATGGAA GATTTCGCGC CCGCGCGTGC TGGCGCACGA CTACGGCGGC GCGACGGTGC TGCGCGCGCA TTTCCTCGAC GGCATCGCGT ATTCCGACCT GACGCTCGTG AATCCCGTCG CGATCGCGCC GCAGGGCTCG CCGTTCGTGC GGCATGTCGC GCAGCACGAG GCCGCGTTCA CCGGGTTGCC GGCCTACGCG CATCACGCGC TCGTGTCGGC CTATATCGGC CAAGCGGTCG CGCAGCCGTT GAGCGACGAC GTGCTGTCGA TCTACCGCGC GCCATGGCTC ACGCCCGCGG GCCAGGCCGC GTTCTATCGG CAAATCGCGC AGATGCGCCA GCGCTACATC GAGGATGCGG AGGCACGCTA CGCGCCGCCG GACTTTCCGG TGCGCATCGT CTGGGGCGAG GACGACCGGT GGATTCCGCT CGAACAGGGG CAGGCGCTGG CCGATCGCAT CGCGAACGGC AAGCTGATCA GGGTGCCGCG CGCCGGTCAC CTGGTCCAGG AAGATGCGCC GGAGGCGATC GTCGCCGCCG TGCTCGACCG G
Details for BuamA.00095.a.B1.GE39927
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/2/2016 |
| EXPECTED MW: | 31kDa |
| OBSERVED MW: | 31kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHML DLANRFNFEG HRIAWGTLGE GPPLVLVHGT PFSSQVWRRI APWLARRHRV FFYDLLGYGQ SDMPDADVSL GRQNVLFGAL LDEWKISRPR VLAHDYGGAT VLRAHFLDGI AYSDLTLVNP VAIAPQGSPF VRHVAQHEAA FTGLPAYAHH ALVSAYIGQA VAQPLSDDVL SIYRAPWLTP AGQAAFYRQI AQMRQRYIED AEARYAPPDF PVRIVWGEDD RWIPLEQGQA LADRIANGKL IRVPRAGHLV QEDAPEAIVA AVLDR
Validated NT Sequence
atcttactca cttaccggtc gagcacggcg gcgacgatcg cctccggcgc atcttcctgg accaggtgac cggcgcgcgg caccctgatc agcttgccgt tcgcgatgcg atcggccagc gcctgcccct gttcgagcgg aatccaccgg tcgtcctcgc cccagacgat gcgcaccgga aagtccggcg gcgcgtagcg tgcctccgca tcctcgatgt agcgctggcg catctgcgcg atttgccgat agaacgcggc ctggcccgcg ggcgtgagcc atggcgcgcg gtagatcgac agcacgtcgt cgctcaacgg ctgcgcgacc gcttggccga tataggccga cacgagcgcg tgatgcgcgt aggccggcaa cccggtgaac gcggcctcgt gctgcgcgac atgccgcacg aacggcgagc cctgcggcgc gatcgcgacg ggattcacga gcgtcaggtc ggaatacgcg atgccgtcga ggaaatgcgc gcgcagcacc gtcgcgccgc cgtagtcgtg cgccagcacg cgcgggcgcg aaatcttcca ttcgtcgagc agcgcgccga acaacacgtt ctgccggccg agcgatacgt ccgcgtcggg catgtcggac tggccgtagc cgagcaggtc gtagaagaac acgcgatggc gccgcgcgag ccacggcgcg atccggcgcc acacctgcga tgaaaacggc gtgccgtgca caagcacgag cggcggccct tcgccgagcg tgccccaagc gatccggtga ccttcgaaat tgaagcgatt ggcgagatcg agcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHML DLANRFNFEG HRIAWGTLGE GPPLVLVHGT PFSSQVWRRI APWLARRHRV FFYDLLGYGQ SDMPDADVSL GRQNVLFGAL LDEWKISRPR VLAHDYGGAT VLRAHFLDGI AYSDLTLVNP VAIAPQGSPF VRHVAQHEAA FTGLPAYAHH ALVSAYIGQA VAQPLSDDVL SIYRAPWLTP AGQAAFYRQI AQMRQRYIED AEARYAPPDF PVRIVWGEDD RWIPLEQGQA LADRIANGKL IRVPRAGHLV QEDAPEAIVA AVLDR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctcg atctcgccaa tcgcttcaat ttcgaaggtc accggatcgc ttggggcacg ctcggcgaag ggccgccgct cgtgcttgtg cacggcacgc cgttttcatc gcaggtgtgg cgccggatcg cgccgtggct cgcgcggcgc catcgcgtgt tcttctacga cctgctcggc tacggccagt ccgacatgcc cgacgcggac gtatcgctcg gccggcagaa cgtgttgttc ggcgcgctgc tcgacgaatg gaagatttcg cgcccgcgcg tgctggcgca cgactacggc ggcgcgacgg tgctgcgcgc gcatttcctc gacggcatcg cgtattccga cctgacgctc gtgaatcccg tcgcgatcgc gccgcagggc tcgccgttcg tgcggcatgt cgcgcagcac gaggccgcgt tcaccgggtt gccggcctac gcgcatcacg cgctcgtgtc ggcctatatc ggccaagcgg tcgcgcagcc gttgagcgac gacgtgctgt cgatctaccg cgcgccatgg ctcacgcccg cgggccaggc cgcgttctat cggcaaatcg cgcagatgcg ccagcgctac atcgaggatg cggaggcacg ctacgcgccg ccggactttc cggtgcgcat cgtctggggc gaggacgacc ggtggattcc gctcgaacag gggcaggcgc tggccgatcg catcgcgaac ggcaagctga tcagggtgcc gcgcgccggt cacctggtcc aggaagatgc gccggaggcg atcgtcgccg ccgtgctcga ccggtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuamA.00095.a.B1.PS38005
| PURIFICATION DATe: | 8/3/2016 |
| CONCENTRATION: | 39.8mg/ml |
| OBSERVED MW: | 31kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 3 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML DLANRFNFEG HRIAWGTLGE GPPLVLVHGT PFSSQVWRRI APWLARRHRV FFYDLLGYGQ SDMPDADVSL GRQNVLFGAL LDEWKISRPR VLAHDYGGAT VLRAHFLDGI AYSDLTLVNP VAIAPQGSPF VRHVAQHEAA FTGLPAYAHH ALVSAYIGQA VAQPLSDDVL SIYRAPWLTP AGQAAFYRQI AQMRQRYIED AEARYAPPDF PVRIVWGEDD RWIPLEQGQA LADRIANGKL IRVPRAGHLV QEDAPEAIVA AVLDR
Validated NT Sequence
atcttactca cttaccggtc gagcacggcg gcgacgatcg cctccggcgc atcttcctgg accaggtgac cggcgcgcgg caccctgatc agcttgccgt tcgcgatgcg atcggccagc gcctgcccct gttcgagcgg aatccaccgg tcgtcctcgc cccagacgat gcgcaccgga aagtccggcg gcgcgtagcg tgcctccgca tcctcgatgt agcgctggcg catctgcgcg atttgccgat agaacgcggc ctggcccgcg ggcgtgagcc atggcgcgcg gtagatcgac agcacgtcgt cgctcaacgg ctgcgcgacc gcttggccga tataggccga cacgagcgcg tgatgcgcgt aggccggcaa cccggtgaac gcggcctcgt gctgcgcgac atgccgcacg aacggcgagc cctgcggcgc gatcgcgacg ggattcacga gcgtcaggtc ggaatacgcg atgccgtcga ggaaatgcgc gcgcagcacc gtcgcgccgc cgtagtcgtg cgccagcacg cgcgggcgcg aaatcttcca ttcgtcgagc agcgcgccga acaacacgtt ctgccggccg agcgatacgt ccgcgtcggg catgtcggac tggccgtagc cgagcaggtc gtagaagaac acgcgatggc gccgcgcgag ccacggcgcg atccggcgcc acacctgcga tgaaaacggc gtgccgtgca caagcacgag cggcggccct tcgccgagcg tgccccaagc gatccggtga ccttcgaaat tgaagcgatt ggcgagatcg agcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHML DLANRFNFEG HRIAWGTLGE GPPLVLVHGT PFSSQVWRRI APWLARRHRV FFYDLLGYGQ SDMPDADVSL GRQNVLFGAL LDEWKISRPR VLAHDYGGAT VLRAHFLDGI AYSDLTLVNP VAIAPQGSPF VRHVAQHEAA FTGLPAYAHH ALVSAYIGQA VAQPLSDDVL SIYRAPWLTP AGQAAFYRQI AQMRQRYIED AEARYAPPDF PVRIVWGEDD RWIPLEQGQA LADRIANGKL IRVPRAGHLV QEDAPEAIVA AVLDR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTCG ATCTCGCCAA TCGCTTCAAT TTCGAAGGTC ACCGGATCGC TTGGGGCACG CTCGGCGAAG GGCCGCCGCT CGTGCTTGTG CACGGCACGC CGTTTTCATC GCAGGTGTGG CGCCGGATCG CGCCGTGGCT CGCGCGGCGC CATCGCGTGT TCTTCTACGA CCTGCTCGGC TACGGCCAGT CCGACATGCC CGACGCGGAC GTATCGCTCG GCCGGCAGAA CGTGTTGTTC GGCGCGCTGC TCGACGAATG GAAGATTTCG CGCCCGCGCG TGCTGGCGCA CGACTACGGC GGCGCGACGG TGCTGCGCGC GCATTTCCTC GACGGCATCG CGTATTCCGA CCTGACGCTC GTGAATCCCG TCGCGATCGC GCCGCAGGGC TCGCCGTTCG TGCGGCATGT CGCGCAGCAC GAGGCCGCGT TCACCGGGTT GCCGGCCTAC GCGCATCACG CGCTCGTGTC GGCCTATATC GGCCAAGCGG TCGCGCAGCC GTTGAGCGAC GACGTGCTGT CGATCTACCG CGCGCCATGG CTCACGCCCG CGGGCCAGGC CGCGTTCTAT CGGCAAATCG CGCAGATGCG CCAGCGCTAC ATCGAGGATG CGGAGGCACG CTACGCGCCG CCGGACTTTC CGGTGCGCAT CGTCTGGGGC GAGGACGACC GGTGGATTCC GCTCGAACAG GGGCAGGCGC TGGCCGATCG CATCGCGAAC GGCAAGCTGA TCAGGGTGCC GCGCGCCGGT CACCTGGTCC AGGAAGATGC GCCGGAGGCG ATCGTCGCCG CCGTGCTCGA CCGGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT