SSGCID: Target Page

BuamA.00090.b

Phospho-2-dehydro-3-deoxyheptonate aldolase

CENTER ID:	BuamA.00090.b
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00090.b.B1.GE39258	Full length( BuamA.00090.b )		1	376

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.5649
RefSeq:	YP_001812096.1
UniProt:	B1Z2B8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQNLDNPSHD REVGSADATQ DTTRIDDVRI GAVRPLISPA LLQDELPVPA ATQTLVEDTR RAIGDILHGR DDRLLLVVGP CSIHDHDQAL DYARRLKAAA DALKDDLLIT MRVYFEKPRT TVGWKGYIND PRLDGSFRIN EGLRAARQLL LDINALGLPA STEFLDLLSP QYIADLIAWG AIGARTTESQ SHRQLASGLS CPIGFKNGTD GGVQVASDAI VAARASHAFM GMTKMGMAAI FETRGNDDAH VILRGGKNGP NYDAEHVEAS CAVLRKSGLR EQVMVDCSHA NSNKSHERQI DVAQDLARQL GQGEHRIVGV MVESHLEAGR QDLKPGVPLQ YGVSITDACL SWSQTEPVLD VLAEAVRHRR VYSRNA

NT Sequence

ATGCAGAACC TCGACAATCC TTCCCACGAT CGCGAGGTAG GCAGCGCCGA CGCGACGCAG GACACCACGC GCATCGACGA CGTCCGCATC GGCGCCGTGC GTCCGCTGAT TTCCCCCGCT CTGCTGCAGG ACGAGCTGCC GGTGCCGGCC GCCACGCAGA CGCTCGTCGA GGACACCCGC CGCGCGATCG GCGACATCCT GCATGGCCGC GACGACCGGC TGCTGCTCGT CGTCGGCCCG TGCTCGATCC ACGACCACGA TCAGGCGCTC GACTACGCAC GCCGCCTGAA GGCTGCCGCC GATGCGCTGA AGGACGACCT GCTGATCACG ATGCGCGTGT ACTTCGAGAA GCCGCGCACG ACGGTCGGCT GGAAGGGATA CATCAACGAT CCGCGCCTGG ACGGCAGTTT CCGCATCAAC GAAGGGCTGC GCGCCGCGCG GCAGCTGCTG CTCGACATCA ACGCGCTGGG CCTGCCGGCG TCGACCGAAT TCCTCGATTT GCTGAGCCCG CAATACATTG CCGACCTGAT CGCGTGGGGC GCGATCGGCG CGCGCACGAC CGAGAGCCAG AGCCATCGCC AGCTCGCGTC GGGGCTGAGC TGCCCGATCG GCTTCAAGAA CGGCACCGAC GGCGGCGTGC AGGTGGCGTC GGACGCGATC GTCGCCGCGC GCGCGAGCCA TGCGTTCATG GGGATGACGA AGATGGGGAT GGCCGCGATC TTCGAGACGC GCGGCAACGA CGACGCGCAC GTGATCCTGC GCGGCGGCAA GAACGGGCCG AACTACGACG CCGAGCACGT CGAGGCGAGC TGCGCGGTGC TGCGCAAGAG CGGGTTGCGC GAGCAGGTGA TGGTCGATTG CTCGCATGCG AACTCGAACA AGTCGCATGA GCGGCAGATC GACGTCGCGC AGGATCTCGC GCGACAGTTG GGGCAGGGCG AGCACCGGAT CGTCGGCGTG ATGGTGGAGA GCCATCTCGA GGCCGGGCGT CAGGATCTGA AGCCGGGTGT GCCGTTGCAG TACGGCGTGT CGATCACCGA TGCGTGCCTG AGCTGGTCGC AGACCGAGCC GGTGCTCGAC GTGCTGGCCG AGGCGGTGCG GCATCGCCGG GTGTATTCGC GCAACGCG

Details for BuamA.00090.b.B1.GE39258

HARVESTED ON:	6/10/2015
SEQUENCED ON:	6/5/2015
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMQ NLDNPSHDRE VGSADATQDT TRIDDVRIGA VRPLISPALL QDELPVPAAT QTLVEDTRRA IGDILHGRDD RLLLVVGPCS IHDHDQALDY ARRLKAAADA LKDDLLITMR VYFEKPRTTV GWKGYINDPR LDGSFRINEG LRAARQLLLD INALGLPAST EFLDLLSPQY IADLIAWGAI GARTTESQSH RQLASGLSCP IGFKNGTDGG VQVASDAIVA ARASHAFMGM TKMGMAAIFE TRGNDDAHVI LRGGKNGPNY DAEHVEASCA VLRKSGLREQ VMVDCSHANS NKSHERQIDV AQDLARQLGQ GEHRIVGVMV ESHLEAGRQD LKPGVPLQYG VSITDACLSW SQTEPVLDVL AEAVR

Validated NT Sequence

atgccgcacc gcctcggcca gcacgtcgag caccggctcg gtctgcgacc agctcaggca cgcatcggtg atcgacacgc cgtactgcaa cggcacaccc ggcttcagat cctgacgccc ggcctcgaga tggctctcca ccatcacgcc gacgatccgg tgctcgccct gccccaactg tcgcgcgaga tcctgcgcga cgtcgatctg ccgctcatgc gacttgttcg agttcgcatg cgagcaatcg accatcacct gctcgcgcaa cccgctcttg cgcagcaccg cgcagctcgc ctcgacgtgc tcggcgtcgt agttcggccc gttcttgccg ccgcgcagga tcacgtgcgc gtcgtcgttg ccgcgcgtct cgaagatcgc ggccatcccc atcttcgtca tccccatgaa cgcatggctc gcgcgcgcgg cgacgatcgc gtccgacgcc acctgcacgc cgccgtcggt gccgttcttg aagccgatcg ggcagctcag ccccgacgcg agctggcgat ggctctggct ctcggtcgtg cgcgcgccga tcgcgcccca cgcgatcagg tcggcaatgt attgcgggct cagcaaatcg aggaattcgg tcgacgccgg caggcccagc gcgttgatgt cgagcagcag ctgccgcgcg gcgcgcagcc cttcgttgat gcggaaactg ccgtccaggc gcggatcgtt gatgtatccc ttccagccga ccgtcgtgcg cggcttctcg aagtacacgc gcatcgtgat cagcaggtcg tccttcagcg catcggcggc agccttcagg cggcgtgcgt agtcgagcgc ctgatcgtgg tcgtggatcg agcacgggcc gacgacgagc agcagccggt cgtcgcggcc atgcaggatg tcgccgatcg cgcggcgggt gtcctcgacg agcgtctgcg tggcggccgg caccggcagc tcgtcctgca gcagagcggg ggaaatcagc ggacgcacgg cgccgatgcg gacgtcgtcg atgcgcgtgg tgtcctgcgt cgcgtcggcg ctgcctacct cgcgatcgtg ggaaggattg tcgaggttct gcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga acctcgacaa tccttcccac gatcgcgagg taggcagcgc cgacgcgacg caggacacca cgcgcatcga cgacgtccgc atcggcgccg tgcgtccgct gatttccccc gctctgctgc aggacgagct gccggtgccg gccgccacgc agacgctcgt cgaggacacc cgccgcgcga tcggcgacat cctgcatggc cgcgacgacc ggctgctgct cgtcgtcggc ccgtgctcga tccacgacca cgatcaggcg ctcgactacg cacgccgcct gaaggctgcc gccgatgcgc tgaaggacga cctgctgatc acgatgcgcg tgtacttcga gaagccgcgc acgacggtcg gctggaaggg atacatcaac gatccgcgcc tggacggcag tttccgcatc aacgaagggc tgcgcgccgc gcggcagctg ctgctcgaca tcaacgcgct gggcctgccg gcgtcgaccg aattcctcga tttgctgagc ccgcaataca ttgccgacct gatcgcgtgg ggcgcgatcg gcgcgcgcac gaccgagagc cagagccatc gccagctcgc gtcggggctg agctgcccga tcggcttcaa gaacggcacc gacggcggcg tgcaggtggc gtcggacgcg atcgtcgccg cgcgcgcgag ccatgcgttc atggggatga cgaagatggg gatggccgcg atcttcgaga cgcgcggcaa cgacgacgcg cacgtgatcc tgcgcggcgg caagaacggg ccgaactacg acgccgagca cgtcgaggcg agctgcgcgg tgctgcgcaa gagcgggttg cgcgagcagg tgatggtcga ttgctcgcat gcgaactcga acaagtcgca tgagcggcag atcgacgtcg cgcaggatct cgcgcgacag ttggggcagg gcgagcaccg gatcgtcggc gtgatggtgg agagccatct cgaggccggg cgtcaggatc tgaagccggg tgtgccgttg cagtacggcg tgtcgatcac cgatgcgtgc ctgagctggt cgcagaccga gccggtgctc gacgtgctgg ccgaggcggt gcggcatcgc cgggtgtatt cgcgcaacgc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat