SSGCID: Target Page

BuamA.00090.a

Phospho-2-dehydro-3-deoxyheptonate aldolase

CENTER ID:	BuamA.00090.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00090.a.B1.GE39257	Full length( BuamA.00090.a )		1	357

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.662
RefSeq:	YP_001807368.1
UniProt:	B1YTI7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MPPHNTDDVR IRELKELTPP AHLIREFACS EAASELIYHS RQSMHRILHG MDDRLIVVIG PCSIHDTKAA IEYAGRLVKE RERFKGELEI VMRVYFEKPR TTVGWKGLIN DPHLDNSFKI NEGLRTAREL LLHINEMGLP AATEYLDMIS PQYIADLISW GAIGARTTES QVHRELASGL SCPVGFKNGT DGNVKIAVDA IKAASQPHHF LSVTKGGHSA IVSTAGNEDC HVILRGGKAP NYDADSVNAA CADIGKAGLA ARLMIDASHA NSSKKHENQI PVCADIGRQV AAGDERIVGV MIESHLVEGR QDLKEGCPLT YGQSITDACI AWDDSVKVLE GLAESVKARR VARGSGN

NT Sequence

ATGCCCCCGC ACAACACCGA CGATGTCCGC ATTCGCGAAC TCAAGGAACT GACGCCGCCC GCCCACCTGA TCCGCGAATT CGCGTGCTCC GAGGCCGCGT CCGAGCTGAT CTACCACTCG CGCCAGTCGA TGCACCGCAT CCTGCACGGG ATGGACGACC GGCTGATCGT CGTGATCGGG CCGTGCTCGA TCCACGATAC GAAGGCGGCG ATCGAGTACG CGGGGCGGCT CGTGAAGGAG CGCGAGCGCT TCAAGGGCGA GCTGGAAATC GTGATGCGCG TGTACTTCGA AAAGCCGCGC ACGACGGTCG GCTGGAAGGG GCTCATCAAC GATCCGCACC TGGACAACAG CTTCAAGATC AACGAAGGGC TGCGCACCGC GCGTGAGTTG CTGCTGCATA TCAACGAGAT GGGGCTGCCG GCCGCGACCG AATACCTCGA CATGATCAGC CCGCAGTACA TCGCCGACCT GATCTCGTGG GGCGCGATCG GCGCGCGCAC GACCGAGTCG CAGGTGCACC GCGAGCTGGC GTCGGGGCTG TCGTGCCCGG TCGGCTTCAA GAACGGCACC GACGGCAACG TGAAGATCGC GGTCGACGCG ATCAAGGCCG CGTCCCAGCC GCACCATTTC CTGTCGGTGA CCAAGGGCGG CCACTCGGCG ATCGTGTCGA CGGCCGGCAA CGAGGACTGC CACGTGATCC TGCGCGGCGG CAAGGCGCCG AACTACGACG CGGACAGCGT GAACGCCGCG TGCGCGGACA TCGGCAAGGC CGGCCTCGCC GCGCGCCTGA TGATCGACGC GAGCCACGCG AACAGCTCGA AGAAGCACGA GAACCAGATT CCCGTGTGCG CGGACATCGG CCGCCAGGTC GCGGCCGGCG ACGAGCGCAT CGTCGGCGTG ATGATCGAGT CGCACCTCGT CGAGGGGCGT CAGGACCTGA AGGAAGGCTG CCCGCTGACG TACGGCCAGA GCATCACCGA TGCGTGCATC GCGTGGGACG ACAGCGTGAA GGTGCTCGAA GGCCTCGCGG AATCGGTGAA GGCACGCCGC GTCGCGCGCG GCAGCGGGAA C

Details for BuamA.00090.a.B1.GE39257

HARVESTED ON:	6/10/2015
SEQUENCED ON:	6/5/2015
EXPECTED MW:	40kDa
OBSERVED MW:	40kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	98
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMP PHNTDDVRIR ELKELTPPAH LIREFACSEA ASELIYHSRQ SMHRILHGMD DRLIVVIGPC SIHDTKAAIE YAGRLVKERE RFKGELEIVM RVYFEKPRTT VGWKGLINDP HLDNSFKINE GLRTARELLL HINEMGLPAA TEYLDMISPQ YIADLISWGA IGARTTESQV HRELASGLSC PVGFKNGTDG NVKIAVDAIK AASQPHHFLS VTKGGHSAIV STAGNEDCHV ILRGGKAPNY DADSVNAACA DIGKAGLAAR LMIDASHANS SKKHENQIPV CADIGRQVAA GDERIVGVMI ESHLVEGRQD LKEGCPLTYG QSITDACIAW DDXVKVXXXL AESVKA

Validated NT Sequence

gcgtgccttc accgattccg cgagnncttn nngcaccttc acgntgtcgt cccacgcgat gcacgcatcg gtgatgctct ggccgtacgt cagcgggcag ccttccttca ggtcctgacg cccctcgacg aggtgcgact cgatcatcac gccgacgatg cgctcgtcgc cggccgcgac ctggcggccg atgtccgcgc acacgggaat ctggttctcg tgcttcttcg agctgttcgc gtggctcgcg tcgatcatca ggcgcgcggc gaggccggcc ttgccgatgt ccgcgcacgc ggcgttcacg ctgtccgcgt cgtagttcgg cgccttgccg ccgcgcagga tcacgtggca gtcctcgttg ccggccgtcg acacgatcgc cgagtggccg cccttggtca ccgacaggaa atggtgcggc tgggacgcgg ccttgatcgc gtcgaccgcg atcttcacgt tgccgtcggt gccgttcttg aagccgaccg ggcacgacag ccccgacgcc agctcgcggt gcacctgcga ctcggtcgtg cgcgcgccga tcgcgcccca cgagatcagg tcggcgatgt actgcgggct gatcatgtcg aggtattcgg tcgcggccgg cagccccatc tcgttgatat gcagcagcaa ctcacgcgcg gtgcgcagcc cttcgttgat cttgaagctg ttgtccaggt gcggatcgtt gatgagcccc ttccagccga ccgtcgtgcg cggcttttcg aagtacacgc gcatcacgat ttccagctcg cccttgaagc gctcgcgctc cttcacgagc cgccccgcgt actcgatcgc cgccttcgta tcgtggatcg agcacggccc gatcacgacg atcagccggt cgtccatccc gtgcaggatg cggtgcatcg actggcgcga gtggtagatc agctcggacg cggcctcgga gcacgcgaat tcgcggatca ggtgggcggg cggcgtcagt tccttgagtt cgcgaatgcg gacatcgtcg gtgttgtgcg ggggcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccc cgcacaacac cgacgatgtc cgcattcgcg aactcaagga actgacgccg cccgcccacc tgatccgcga attcgcgtgc tccgaggccg cgtccgagct gatctaccac tcgcgccagt cgatgcaccg catcctgcac gggatggacg accggctgat cgtcgtgatc gggccgtgct cgatccacga tacgaaggcg gcgatcgagt acgcggggcg gctcgtgaag gagcgcgagc gcttcaaggg cgagctggaa atcgtgatgc gcgtgtactt cgaaaagccg cgcacgacgg tcggctggaa ggggctcatc aacgatccgc acctggacaa cagcttcaag atcaacgaag ggctgcgcac cgcgcgtgag ttgctgctgc atatcaacga gatggggctg ccggccgcga ccgaatacct cgacatgatc agcccgcagt acatcgccga cctgatctcg tggggcgcga tcggcgcgcg cacgaccgag tcgcaggtgc accgcgagct ggcgtcgggg ctgtcgtgcc cggtcggctt caagaacggc accgacggca acgtgaagat cgcggtcgac gcgatcaagg ccgcgtccca gccgcaccat ttcctgtcgg tgaccaaggg cggccactcg gcgatcgtgt cgacggccgg caacgaggac tgccacgtga tcctgcgcgg cggcaaggcg ccgaactacg acgcggacag cgtgaacgcc gcgtgcgcgg acatcggcaa ggccggcctc gccgcgcgcc tgatgatcga cgcgagccac gcgaacagct cgaagaagca cgagaaccag attcccgtgt gcgcggacat cggccgccag gtcgcggccg gcgacgagcg catcgtcggc gtgatgatcg agtcgcacct cgtcgagggg cgtcaggacc tgaaggaagg ctgcccgctg acgtacggcc agagcatcac cgatgcgtgc atcgcgtggg acgacagcgt gaaggtgctc gaaggcctcg cggaatcggt gaaggcacgc cgcgtcgcgc gcggcagcgg gaactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat