BuamA.00088.a

Ornithine carbamoyltransferase

CENTER ID: BuamA.00088.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00088.a.A1.GE32120 Full length( BuamA.00088.a ) 1 309
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00088.a.A1.PW32038 Full length( BuamA.00088.a ) 1 309

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2543
RefSeq: YP_001809166.1
UniProt: B1YVE6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTAKTIRHYL QFKDFSLEDY EYVLERTGIL KRKFKNYETY HPLHDRTLAM IFEKSSTRTR LSFEAGIFQL GGHAVFMSTR DTQLGRGEPV EDSAQVISRM VDIIMIRTFE QDVITRFAQN SRVPVINGLT NDYHPCQVLA DIFTYYEHRG PIAGKTVAWV GDANNMLYTW IEAAQILGFK LRLSTPPGYA LDMKLVSPDS APFYEVFDDP NEACKGADLV TTDVWTSMGF EAENEARKQA FADWCVDEEM MGHANADALF MHCLPAHRGE EVTAGVIDGP QSVVWDEAEN RLHVQKALME FLLLGRLKH
NT Sequence
ATGACCGCCA AAACCATTCG TCACTACCTG CAGTTCAAGG ATTTCTCGCT GGAAGACTAC GAGTACGTGC TCGAACGCAC GGGTATCCTG AAGCGCAAGT TCAAGAACTA CGAGACCTAT CACCCGCTGC ACGACCGCAC GCTCGCGATG ATCTTCGAGA AGAGCTCGAC GCGCACGCGG CTGTCGTTCG AAGCCGGCAT CTTCCAGTTG GGCGGCCACG CGGTGTTCAT GAGCACGCGC GACACGCAGC TCGGCCGCGG CGAGCCCGTC GAGGATTCCG CGCAGGTCAT CTCGCGAATG GTCGACATCA TCATGATCCG CACGTTCGAG CAAGACGTGA TCACGCGCTT CGCGCAGAAC TCCCGCGTGC CGGTGATCAA TGGCCTGACC AACGATTATC ACCCGTGCCA GGTGCTCGCC GACATCTTCA CGTACTACGA GCACCGCGGC CCGATCGCCG GCAAGACGGT CGCGTGGGTC GGCGATGCGA ACAACATGCT GTACACGTGG ATCGAAGCCG CGCAGATCCT CGGCTTCAAG CTGCGCCTGT CGACGCCGCC GGGCTATGCG CTCGACATGA AGCTCGTGTC GCCGGACAGC GCGCCGTTCT ACGAAGTGTT CGACGATCCG AACGAAGCGT GCAAGGGTGC CGATCTCGTG ACGACCGACG TGTGGACGAG CATGGGTTTC GAAGCCGAGA ACGAAGCGCG CAAGCAGGCG TTTGCCGACT GGTGCGTCGA CGAGGAAATG ATGGGCCACG CGAACGCGGA CGCGCTCTTC ATGCACTGCC TGCCCGCGCA CCGCGGCGAG GAAGTGACGG CCGGCGTGAT CGACGGCCCG CAGAGCGTCG TGTGGGACGA GGCGGAAAAC CGCCTGCACG TGCAGAAGGC GCTGATGGAG TTCCTGCTGC TCGGCCGCCT CAAGCAC
Details for BuamA.00088.a.A1.GE32120
HARVESTED ON: 4/26/2011
SEQUENCED ON: 5/2/2011
EXPECTED MW: 37kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MTAKTIRHYL QFKDFSLEDY EYVLERTGIL KRKFKNYETY HPLHDRTLAM IFEKSSTRTR LSFEAGIFQL GGHAVFMSTR DTQLGRGEPV EDSAQVISRM VDIIMIRTFE QDVITRFAQN SRVPVINGLT NDYHPCQVLA DIFTYYEHRG PIAGKTVAWV GDANNMLYTW IEAAQILGFK LRLSTPPGYA LDMKLVSPDS APFYEVFDDP NEACKGADLV TTDVWTSMGF EAENEARKQA FADWCVDEEM MGHANADALF MHCLPAHRGE EVTAGVIDGP QSVVWDEAEN RLHVQKALME FLLLGRL
Validated NT Sequence
tgaggcggcc gagcagcagg aactccatca gcgccttctg cacgtgcagg cggttttccg cctcgtccca cacgacgctc tgcgggccgt cgatcacgcc ggccgtcact tcctcgccgc ggtgcgcggg caggcagtgc atgaagagcg cgtccgcgtt cgcgtggccc atcatttcct cgtcgacgca ccagtcggca aacgcctgct tgcgcgcttc gttctcggct tcgaaaccca tgctcgtcca cacgtcggtc gtcacgagat cggcaccctt gcacgcttcg ttcggatcgt cgaacacttc gtagaacggc gcgctgtccg gcgacacgag cttcatgtcg agcgcatagc ccggcggcgt cgacaggcgc agcttgaagc cgaggatctg cgcggcttcg atccacgtgt acagcatgtt gttcgcatcg ccgacccacg cgaccgtctt gccggcgatc gggccgcggt gctcgtagta cgtgaagatg tcggcgagca cctggcacgg gtgataatcg ttggtcaggc cattgatcac cggcacgcgg gagttctgcg cgaagcgcgt gatcacgtct tgctcgaacg tgcggatcat gatgatgtcg accattcgcg agatgacctg cgcggaatcc tcgacgggct cgccgcggcc gagctgcgtg tcgcgcgtgc tcatgaacac cgcgtggccg cccaactgga agatgccggc ttcgaacgac agccgcgtgc gcgtcgagct cttctcgaag atcatcgcga gcgtgcggtc gtgcagcggg tgataggtct cgtagttctt gaacttgcgc ttcaggatac ccgtgcgttc gagcacgtac tcgtagtctt ccagcgagaa atccttgaac tgcaggtagt gacgaatggt tttggcggtc atcgaaccag gaccctgggt ctgagcttcc agggtac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDVITRFAQ NSRVPVINGL TNDYHPCQVL ADIFTYYEHR GPIAGKTVAW VGDANNMLYT WIEAAQILGF KLRLSTPPGY ALDMKLVSPD SAPFYEVFDD PNEACKGADL VTTDVWTSMG FEAENEARKQ AFADWCVDEE MMGHANADAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRLKH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgccaaa accattcgtc actacctgca gttcaaggat ttctcgctgg aagactacga gtacgtgctc gaacgcacgg gtatcctgaa gcgcaagttc aagaactacg agacctatca cccgctgcac gaccgcacgc tcgcgatgat cttcgagaag agctcgacgc gcacgcggct gtcgttcgaa gccggcatct tccagttggg cggccacgcg gtgttcatga gcacgcgcga cacgcagctc ggccgcggcg agcccgtcga ggattccgcg caggtcatct cgcgaatggt cgacatcatc atgatccgca cgttcgagca agacgtgatc acgcgcttcg cgcagaactc ccgcgtgccg gtgatcaatg gcctgaccaa cgattatcac ccgtgccagg tgctcgccga catcttcacg tactacgagc accgcggccc gatcgccggc aagacggtcg cgtgggtcgg cgatgcgaac aacatgctgt acacgtggat cgaagccgcg cagatcctcg gcttcaagct gcgcctgtcg acgccgccgg gctatgcgct cgacatgaag ctcgtgtcgc cggacagcgc gccgttctac gaagtgttcg acgatccgaa cgaagcgtgc aagggtgccg atctcgtgac gaccgacgtg tggacgagca tgggtttcga agccgagaac gaagcgcgca agcaggcgtt tgccgactgg tgcgtcgacg aggaaatgat gggccacgcg aacgcggacg cgctcttcat gcactgcctg cccgcgcacc gcggcgagga agtgacggcc ggcgtgatcg acggcccgca gagcgtcgtg tgggacgagg cggaaaaccg cctgcacgtg cagaaggcgc tgatggagtt cctgctgctc ggccgcctca agcactaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa
Details for BuamA.00088.a.A1.PW32038
PURIFICATION DATe: 6/10/2011
CONCENTRATION: 29.24mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 24
VIAL VOLUME: 100µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MTAKTIRHYL QFKDFSLEDY EYVLERTGIL KRKFKNYETY HPLHDRTLAM IFEKSSTRTR LSFEAGIFQL GGHAVFMSTR DTQLGRGEPV EDSAQVISRM VDIIMIRTFE QDVITRFAQN SRVPVINGLT NDYHPCQVLA DIFTYYEHRG PIAGKTVAWV GDANNMLYTW IEAAQILGFK LRLSTPPGYA LDMKLVSPDS APFYEVFDDP NEACKGADLV TTDVWTSMGF EAENEARKQA FADWCVDEEM MGHANADALF MHCLPAHRGE EVTAGVIDGP QSVVWDEAEN RLHVQKALME FLLLGRL
Validated NT Sequence
tgaggcggcc gagcagcagg aactccatca gcgccttctg cacgtgcagg cggttttccg cctcgtccca cacgacgctc tgcgggccgt cgatcacgcc ggccgtcact tcctcgccgc ggtgcgcggg caggcagtgc atgaagagcg cgtccgcgtt cgcgtggccc atcatttcct cgtcgacgca ccagtcggca aacgcctgct tgcgcgcttc gttctcggct tcgaaaccca tgctcgtcca cacgtcggtc gtcacgagat cggcaccctt gcacgcttcg ttcggatcgt cgaacacttc gtagaacggc gcgctgtccg gcgacacgag cttcatgtcg agcgcatagc ccggcggcgt cgacaggcgc agcttgaagc cgaggatctg cgcggcttcg atccacgtgt acagcatgtt gttcgcatcg ccgacccacg cgaccgtctt gccggcgatc gggccgcggt gctcgtagta cgtgaagatg tcggcgagca cctggcacgg gtgataatcg ttggtcaggc cattgatcac cggcacgcgg gagttctgcg cgaagcgcgt gatcacgtct tgctcgaacg tgcggatcat gatgatgtcg accattcgcg agatgacctg cgcggaatcc tcgacgggct cgccgcggcc gagctgcgtg tcgcgcgtgc tcatgaacac cgcgtggccg cccaactgga agatgccggc ttcgaacgac agccgcgtgc gcgtcgagct cttctcgaag atcatcgcga gcgtgcggtc gtgcagcggg tgataggtct cgtagttctt gaacttgcgc ttcaggatac ccgtgcgttc gagcacgtac tcgtagtctt ccagcgagaa atccttgaac tgcaggtagt gacgaatggt tttggcggtc atcgaaccag gaccctgggt ctgagcttcc agggtac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTAKTIRHY LQFKDFSLED YEYVLERTGI LKRKFKNYET YHPLHDRTLA MIFEKSSTRT RLSFEAGIFQ LGGHAVFMST RDTQLGRGEP VEDSAQVISR MVDIIMIRTF EQDVITRFAQ NSRVPVINGL TNDYHPCQVL ADIFTYYEHR GPIAGKTVAW VGDANNMLYT WIEAAQILGF KLRLSTPPGY ALDMKLVSPD SAPFYEVFDD PNEACKGADL VTTDVWTSMG FEAENEARKQ AFADWCVDEE MMGHANADAL FMHCLPAHRG EEVTAGVIDG PQSVVWDEAE NRLHVQKALM EFLLLGRLKH
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCCAAA ACCATTCGTC ACTACCTGCA GTTCAAGGAT TTCTCGCTGG AAGACTACGA GTACGTGCTC GAACGCACGG GTATCCTGAA GCGCAAGTTC AAGAACTACG AGACCTATCA CCCGCTGCAC GACCGCACGC TCGCGATGAT CTTCGAGAAG AGCTCGACGC GCACGCGGCT GTCGTTCGAA GCCGGCATCT TCCAGTTGGG CGGCCACGCG GTGTTCATGA GCACGCGCGA CACGCAGCTC GGCCGCGGCG AGCCCGTCGA GGATTCCGCG CAGGTCATCT CGCGAATGGT CGACATCATC ATGATCCGCA CGTTCGAGCA AGACGTGATC ACGCGCTTCG CGCAGAACTC CCGCGTGCCG GTGATCAATG GCCTGACCAA CGATTATCAC CCGTGCCAGG TGCTCGCCGA CATCTTCACG TACTACGAGC ACCGCGGCCC GATCGCCGGC AAGACGGTCG CGTGGGTCGG CGATGCGAAC AACATGCTGT ACACGTGGAT CGAAGCCGCG CAGATCCTCG GCTTCAAGCT GCGCCTGTCG ACGCCGCCGG GCTATGCGCT CGACATGAAG CTCGTGTCGC CGGACAGCGC GCCGTTCTAC GAAGTGTTCG ACGATCCGAA CGAAGCGTGC AAGGGTGCCG ATCTCGTGAC GACCGACGTG TGGACGAGCA TGGGTTTCGA AGCCGAGAAC GAAGCGCGCA AGCAGGCGTT TGCCGACTGG TGCGTCGACG AGGAAATGAT GGGCCACGCG AACGCGGACG CGCTCTTCAT GCACTGCCTG CCCGCGCACC GCGGCGAGGA AGTGACGGCC GGCGTGATCG ACGGCCCGCA GAGCGTCGTG TGGGACGAGG CGGAAAACCG CCTGCACGTG CAGAAGGCGC TGATGGAGTT CCTGCTGCTC GGCCGCCTCA AGCACTAAAC AGCACGAACA AGTTCTGCAG CCAAGCTTCT CGAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAA