BuamA.00081.a

Aminotransferase class V

CENTER ID: BuamA.00081.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00081.a.B1.GE43965 Full length( BuamA.00081.a ) 1 494
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.3467
RefSeq: YP_001810048.1
UniProt: B1YYX2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDTTPLHYL DYAATTPADP RVIEAMTACL GADGIFGNPA SSSHAAGRLA KEKVEHARSQ VAALIGAHAD EIIWTSGATE SNNLALKGYA EAATDKRHLI TSRIEHKAIL DTMANLSKRG MTAAYLNPTA DGEITVDAVA AAIRPDTGLV SLMLVNNELG TLTDIGAISR IVHAAGALLH VDAAQALGKT PIDVRALGID LLSMSAHKVY GPKGIGALFV RRDIADRIAP QIHGGGHERG LRSGTLATHQ IVGMGTACEL AAEKLEREAE RIAALSARLK DALLALGDIT HNAAAARRIP HTLSLTVNTP GFFPFMLGDS LAVSSTSACN STSGAPSHVL TAIGLDAETA GRTVRISFGR FTTEQDVDFA VACFARAIEQ CRAAAATGFT ASRQITPADL KAIRNAGFRA VICNRPDGEG ADQPAFDEIA AAARELGLDA RYLPVERDRI GDAEVDAFGA LVDALPKPVL AYCRSGSRSG MLWNRLTARR TATA
NT Sequence
ATGAGCGATA CCACCCCGCT GCACTACCTC GACTACGCAG CCACCACCCC TGCGGATCCC CGCGTGATCG AAGCGATGAC GGCCTGCCTC GGTGCCGACG GCATCTTCGG CAATCCCGCG TCGAGCTCGC ACGCCGCCGG CCGGCTGGCG AAGGAAAAAG TCGAGCACGC ACGCTCGCAG GTTGCCGCGC TGATCGGCGC GCACGCCGAC GAGATCATCT GGACGTCGGG TGCCACCGAA TCGAACAACC TCGCGCTGAA GGGCTACGCG GAAGCCGCGA CCGACAAGCG CCACCTGATC ACGAGCCGCA TCGAACACAA GGCCATCCTC GACACGATGG CGAACCTGTC GAAGCGCGGG ATGACGGCCG CTTACCTCAA CCCCACCGCC GACGGCGAAA TCACCGTCGA CGCCGTGGCC GCGGCGATCC GCCCCGATAC GGGCCTCGTG TCGCTGATGC TCGTCAACAA CGAGCTCGGC ACGCTGACGG ACATCGGCGC GATCTCGCGC ATCGTGCATG CGGCGGGCGC GCTGCTGCAC GTCGACGCCG CGCAGGCGCT CGGCAAGACG CCGATCGACG TGCGCGCGCT CGGCATCGAC CTGCTGTCGA TGTCCGCGCA CAAGGTGTAT GGGCCAAAGG GTATCGGCGC GCTGTTCGTC CGCCGCGACA TCGCCGATCG CATCGCGCCG CAGATTCACG GCGGCGGGCA CGAACGCGGC CTGCGCTCAG GCACGCTCGC GACGCATCAG ATCGTCGGGA TGGGCACCGC GTGCGAGCTG GCCGCCGAAA AGCTCGAGCG CGAAGCCGAG CGGATCGCGG CACTCAGCGC GCGCCTGAAG GACGCGCTGC TCGCGCTCGG CGACATCACG CACAACGCGG CAGCGGCGCG CCGCATCCCG CACACGTTGA GCCTCACGGT GAACACGCCG GGCTTCTTCC CGTTCATGCT CGGCGATTCG CTCGCCGTGT CGTCGACCTC GGCATGCAAC TCGACGAGCG GCGCGCCGTC CCACGTGCTG ACCGCGATCG GCCTCGATGC GGAAACCGCC GGCCGCACGG TGCGCATCAG CTTCGGCCGC TTCACGACCG AGCAGGACGT CGATTTCGCG GTCGCGTGTT TCGCGCGCGC GATCGAGCAA TGCCGGGCCG CGGCCGCCAC CGGGTTCACC GCGTCGCGGC AGATCACGCC GGCGGACCTG AAAGCGATCC GCAATGCAGG CTTTCGCGCG GTAATCTGCA ACCGGCCGGA CGGCGAAGGC GCTGATCAGC CGGCCTTCGA CGAGATCGCG GCCGCCGCGC GCGAACTCGG CCTCGACGCG CGCTATCTGC CGGTCGAACG CGACCGGATC GGCGATGCCG AAGTCGACGC GTTCGGCGCG CTGGTCGACG CGCTGCCGAA GCCCGTGCTC GCGTATTGCC GCAGCGGGAG CCGCTCCGGG ATGCTGTGGA ACCGGCTGAC GGCGCGACGA ACGGCAACGG CC
Details for BuamA.00081.a.B1.GE43965
HARVESTED ON: 12/3/2020
SEQUENCED ON: 12/16/2020
EXPECTED MW: 53kDa
OBSERVED MW: 53kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMS DTTPLHYLDY AATTPADPRV IEAMTACLGA DGIFGNPASS SHAAGRLAKE KVEHARSQVA ALIGAHADEI IWTSGATESN NLALKGYAEA ATDKRHLITS RIEHKAILDT MANLSKRGMT AAYLNPTADG EITVDAVAAA IRPDTGLVSL MLVNNELGTL TDIGAISRIV HAAGALLHVD AAQALGKTPI DVRALGIDLL SMSAHKVYGP KGIGALFVRR DIADRIAPQI HGGGHXRGLR SGTLATHQIV GMGTACELAA EKLEREAERI AALSARLKDA LLALGDITHN AAAARRIPHT LSLTVNTPGF FPFMLGDSLA VSSTSACNST SGAPSHVLTA IGLDAETAGR TVRISFGRFT TEQDVDFAVA CFARAIEQCR AAAATGFTAS RQITPADLKA IRNAGFRAVI CNRPDGEGAD QPAFDEIAAA ARELGLDARY LPVERDRIGD AEVDAFGALV DALPKPVLAY CRSGSRSGML WNRLTARRTA TA
Validated NT Sequence
atggctcacc accaccacca ccatatgagc gataccaccc cgctgcacta cctcgactac gcagccacca cccctgcgga tccccgcgtg atcgaagcga tgacggcctg cctcggtgcc gacggcatct tcggcaatcc cgcgtcgagc tcgcacgccg ccggccggct ggcgaaggaa aaagtcgagc acgcacgctc gcaggttgcc gcgctgatcg gcgcgcacgc cgacgagatc atctggacgt cgggtgccac cgaatcgaac aacctcgcgc tgaagggcta cgcggaagcc gcgaccgaca agcgccacct gatcacgagc cgcatcgaac acaaggccat cctcgacacg atggcgaacc tgtcgaagcg cgggatgacg gccgcttacc tcaaccccac cgccgacggc gaaatcaccg tcgacgccgt ggccgcggcg atccgccccg atacgggcct cgtgtcgctg atgctcgtca acaacgagct cggcacgctg acggacatcg gcgcgatctc gcgcatcgtg catgcggcgg gcgcgctgct gcacgtcgac gccgcgcagg cgctcggcaa gacgccgatc gacgtgcgcg cgctcggcat cgacctgctg tcgatgtccg cgcacaaggt gtatgggcca aangggtatc ggcgcgctgt tcgtccgccg cgacatcgcc gatcgcatcg cgccgcagat tcacggcggc gggcacgnac gcggcctgcg ctcaggcacg ctcgcgacgc atcagatcgt cgggatgggc accgcgtgcg agctggccgc cgaaaagctc gagcgcgaag ccgagcggat cgcgncactc agcgcgcgcc tgaaggacgc gctgctcgcg ctcggcgaca tcacgcacaa cgcggcagcg gcgcgccgca tcccgcacac gttgagcctc acggtgaaca cgccgggctt cttcccgttc atgctcggcg attcgctcgc cgtgtcgtcg acctcggcat gcaactcgac gagcggcgcg ccgtcccacg tgctgaccgc gatcggcctc gatgcggaaa ccgccggccg cacggtgcgc atcagcttcg gccgcttcac gaccgagcag gacgtcgatt tcgcggtcgc gtgtttcgcg cgcgcgatcg agcaatgccg ggccgcggcc gccaccgggt tcaccgcgtc gcggcagatc acgccggcgg acctgaaagc gatccgcaat gcaggctttc gcgcggtaat ctgcaaccgg ccggacggcg aaggcgctga tcagccggcc ttcgacgaga tcgcggccgc cgcgcgcgaa ctcggcctcg acgcgcgcta tctgccggtc gaacgcgacc ggatcggcga tgccgaagtc gacgcgttcg gcgcgctggt cgacgcgctg ccgaagcccg tgctcgcgta ttgccgcagc gggagccgct ccgggatgct gtggaaccgg ctgacggcgc gac
Expected Protein Sequence
MAHHHHHHMS DTTPLHYLDY AATTPADPRV IEAMTACLGA DGIFGNPASS SHAAGRLAKE KVEHARSQVA ALIGAHADEI IWTSGATESN NLALKGYAEA ATDKRHLITS RIEHKAILDT MANLSKRGMT AAYLNPTADG EITVDAVAAA IRPDTGLVSL MLVNNELGTL TDIGAISRIV HAAGALLHVD AAQALGKTPI DVRALGIDLL SMSAHKVYGP KGIGALFVRR DIADRIAPQI HGGGHERGLR SGTLATHQIV GMGTACELAA EKLEREAERI AALSARLKDA LLALGDITHN AAAARRIPHT LSLTVNTPGF FPFMLGDSLA VSSTSACNST SGAPSHVLTA IGLDAETAGR TVRISFGRFT TEQDVDFAVA CFARAIEQCR AAAATGFTAS RQITPADLKA IRNAGFRAVI CNRPDGEGAD QPAFDEIAAA ARELGLDARY LPVERDRIGD AEVDAFGALV DALPKPVLAY CRSGSRSGML WNRLTARRTA TA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ataccacccc gctgcactac ctcgactacg cagccaccac ccctgcggat ccccgcgtga tcgaagcgat gacggcctgc ctcggtgccg acggcatctt cggcaatccc gcgtcgagct cgcacgccgc cggccggctg gcgaaggaaa aagtcgagca cgcacgctcg caggttgccg cgctgatcgg cgcgcacgcc gacgagatca tctggacgtc gggtgccacc gaatcgaaca acctcgcgct gaagggctac gcggaagccg cgaccgacaa gcgccacctg atcacgagcc gcatcgaaca caaggccatc ctcgacacga tggcgaacct gtcgaagcgc gggatgacgg ccgcttacct caaccccacc gccgacggcg aaatcaccgt cgacgccgtg gccgcggcga tccgccccga tacgggcctc gtgtcgctga tgctcgtcaa caacgagctc ggcacgctga cggacatcgg cgcgatctcg cgcatcgtgc atgcggcggg cgcgctgctg cacgtcgacg ccgcgcaggc gctcggcaag acgccgatcg acgtgcgcgc gctcggcatc gacctgctgt cgatgtccgc gcacaaggtg tatgggccaa agggtatcgg cgcgctgttc gtccgccgcg acatcgccga tcgcatcgcg ccgcagattc acggcggcgg gcacgaacgc ggcctgcgct caggcacgct cgcgacgcat cagatcgtcg ggatgggcac cgcgtgcgag ctggccgccg aaaagctcga gcgcgaagcc gagcggatcg cggcactcag cgcgcgcctg aaggacgcgc tgctcgcgct cggcgacatc acgcacaacg cggcagcggc gcgccgcatc ccgcacacgt tgagcctcac ggtgaacacg ccgggcttct tcccgttcat gctcggcgat tcgctcgccg tgtcgtcgac ctcggcatgc aactcgacga gcggcgcgcc gtcccacgtg ctgaccgcga tcggcctcga tgcggaaacc gccggccgca cggtgcgcat cagcttcggc cgcttcacga ccgagcagga cgtcgatttc gcggtcgcgt gtttcgcgcg cgcgatcgag caatgccggg ccgcggccgc caccgggttc accgcgtcgc ggcagatcac gccggcggac ctgaaagcga tccgcaatgc aggctttcgc gcggtaatct gcaaccggcc ggacggcgaa ggcgctgatc agccggcctt cgacgagatc gcggccgccg cgcgcgaact cggcctcgac gcgcgctatc tgccggtcga acgcgaccgg atcggcgatg ccgaagtcga cgcgttcggc gcgctggtcg acgcgctgcc gaagcccgtg ctcgcgtatt gccgcagcgg gagccgctcc gggatgctgt ggaaccggct gacggcgcga cgaacggcaa cggcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat