SSGCID: Target Page

BuamA.00061.b

UDP-N-acetylglucosamine 2-epimerase

CENTER ID:	BuamA.00061.b
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00061.b.B1.GE39253	Full length( BuamA.00061.b )		1	394

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.3809
RefSeq:	YP_001810378.1
UniProt:	B1Z0Y2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTVRHENILV TFGTRPEAIK MAPVVRRLKS RVGVRCAVCV TAQHRQMLDQ VLGLFGIVPD FDLDLMRNGQ TLGDLTGQIL TSLDPVLESF RPDLVLVHGD TTTTLGASLA AYYRRIPVGH VEAGLRTGNL YAPWPEEANR KMTGALALHH FAPTETSKAN LLSEGVCADR IHVTGNTVID ALLTVTERLT VEPELRARMR AAFPFLSDER RMILVTGHRR ENFGSGFEQI CNALAAIATR FPECRIVYPM HPNPNVREPV TRMLGDIENI ILIEPQEYLQ FVYLMSRAWL ILTDSGGIQE EAPSLGKPVL VMRDTTERPE AVAAGTVRLV GTDAHRLVSE VTQLWECPDV YRSMSRAHNP YGDGHASERI VDLLTAAPSD SRTAQPWPAG SCEA

NT Sequence

ATGACCGTCC GCCATGAAAA CATTCTCGTC ACTTTCGGCA CGCGCCCGGA AGCCATCAAG ATGGCGCCCG TCGTGCGCCG CCTGAAGTCC CGCGTCGGCG TGCGATGCGC GGTATGCGTG ACCGCGCAGC ATCGGCAGAT GCTCGATCAG GTGCTCGGCC TGTTCGGCAT CGTGCCCGAT TTCGATCTCG ACCTGATGCG CAACGGGCAG ACGCTCGGCG ATCTGACCGG GCAGATCCTG ACGTCGCTCG ATCCGGTGCT CGAGTCGTTC CGGCCGGATC TCGTGCTGGT GCATGGAGAC ACCACGACGA CGCTGGGTGC TTCGCTTGCG GCCTACTACC GCCGCATTCC GGTCGGACAC GTGGAAGCCG GCCTGCGGAC GGGGAACCTG TACGCACCCT GGCCGGAGGA GGCCAACCGC AAAATGACCG GCGCGCTCGC TCTGCATCAT TTCGCGCCGA CCGAGACGTC GAAGGCGAAC CTGCTGTCCG AAGGCGTGTG TGCCGACCGG ATTCACGTGA CGGGCAATAC GGTCATCGAC GCGTTGCTGA CGGTGACCGA ACGATTGACA GTCGAGCCGG AACTGCGCGC ACGAATGCGT GCCGCATTTC CGTTCCTGAG CGACGAGCGC CGAATGATTC TCGTCACCGG GCACCGGCGC GAGAATTTCG GCTCGGGCTT CGAACAGATC TGCAATGCAC TGGCGGCGAT CGCGACGCGT TTTCCCGAGT GCCGGATCGT CTATCCGATG CATCCGAATC CGAACGTCCG CGAGCCGGTG ACACGCATGC TCGGCGACAT TGAAAACATC ATCCTGATCG AGCCTCAGGA GTATCTGCAG TTCGTCTATC TGATGAGTCG CGCGTGGCTG ATCCTGACCG ATTCGGGAGG CATACAGGAG GAAGCGCCGT CGCTCGGCAA GCCGGTCCTC GTGATGCGCG ACACGACGGA ACGTCCGGAA GCCGTCGCGG CCGGCACGGT CCGTCTGGTC GGCACCGACG CGCACCGGCT GGTCAGCGAA GTCACGCAGC TGTGGGAGTG CCCGGACGTA TACCGCTCGA TGAGCCGGGC ACACAACCCA TACGGCGACG GCCACGCAAG CGAGCGCATC GTCGACCTGC TGACGGCCGC CCCGTCCGAT TCCCGCACGG CGCAGCCCTG GCCGGCCGGC TCGTGTGAAG CC

Details for BuamA.00061.b.B1.GE39253

HARVESTED ON:	6/10/2015
SEQUENCED ON:	6/5/2015
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT VRHENILVTF GTRPEAIKMA PVVRRLKSRV GVRCAVCVTA QHRQMLDQVL GLFGIVPDFD LDLMRNGQTL GDLTGQILTS LDPVLESFRP DLVLVHGDTT TTLGASLAAY YRRIPVGHVE AGLRTGNLYA PWPEEANRKM TGALALHHFA PTETSKANLL SEGVCADRIH VTGNTVIDAL LTVTERLTVE PELRARMRAA FPFLSDERRM ILVTGHRREN FGSGFEQICN ALAAIATRFP ECRIVYPMHP NPNVREPVTR MLGDIENIIL IEPQEYLQFV YLMSRAWLIL TDSGGIQEEA PSLGKPVLVM RDTTERPEAV AAGTVRLVGT DAHRLVSEVT QLWECPDVYR SMSRAHNPYG DGHASERIVD LLTAAPSDSR TAQ

Validated NT Sequence

gggctgcgcc gtgcgggaat cggacggggc ggccgtcagc aggtcgacga tgcgctcgct tgcgtggccg tcgccgtatg ggttgtgtgc ccggctcatc gagcggtata cgtccgggca ctcccacagc tgcgtgactt cgctgaccag ccggtgcgcg tcggtgccga ccagacggac cgtgccggcc gcgacggctt ccggacgttc cgtcgtgtcg cgcatcacga ggaccggctt gccgagcgac ggcgcttcct cctgtatgcc tcccgaatcg gtcaggatca gccacgcgcg actcatcaga tagacgaact gcagatactc ctgaggctcg atcaggatga tgttttcaat gtcgccgagc atgcgtgtca ccggctcgcg gacgttcgga ttcggatgca tcggatagac gatccggcac tcgggaaaac gcgtcgcgat cgccgccagt gcattgcaga tctgttcgaa gcccgagccg aaattctcgc gccggtgccc ggtgacgaga atcattcggc gctcgtcgct caggaacgga aatgcggcac gcattcgtgc gcgcagttcc ggctcgactg tcaatcgttc ggtcaccgtc agcaacgcgt cgatgaccgt attgcccgtc acgtgaatcc ggtcggcaca cacgccttcg gacagcaggt tcgccttcga cgtctcggtc ggcgcgaaat gatgcagagc gagcgcgccg gtcattttgc ggttggcctc ctccggccag ggtgcgtaca ggttccccgt ccgcaggccg gcttccacgt gtccgaccgg aatgcggcgg tagtaggccg caagcgaagc acccagcgtc gtcgtggtgt ctccatgcac cagcacgaga tccggccgga acgactcgag caccggatcg agcgacgtca ggatctgccc ggtcagatcg ccgagcgtct gcccgttgcg catcaggtcg agatcgaaat cgggcacgat gccgaacagg ccgagcacct gatcgagcat ctgccgatgc tgcgcggtca cgcataccgc gcatcgcacg ccgacgcggg acttcaggcg gcgcacgacg ggcgccatct tgatggcttc cgggcgcgtg ccgaaagtga cgagaatgtt ttcatggcgg acggtcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg tccgccatga aaacattctc gtcactttcg gcacgcgccc ggaagccatc aagatggcgc ccgtcgtgcg ccgcctgaag tcccgcgtcg gcgtgcgatg cgcggtatgc gtgaccgcgc agcatcggca gatgctcgat caggtgctcg gcctgttcgg catcgtgccc gatttcgatc tcgacctgat gcgcaacggg cagacgctcg gcgatctgac cgggcagatc ctgacgtcgc tcgatccggt gctcgagtcg ttccggccgg atctcgtgct ggtgcatgga gacaccacga cgacgctggg tgcttcgctt gcggcctact accgccgcat tccggtcgga cacgtggaag ccggcctgcg gacggggaac ctgtacgcac cctggccgga ggaggccaac cgcaaaatga ccggcgcgct cgctctgcat catttcgcgc cgaccgagac gtcgaaggcg aacctgctgt ccgaaggcgt gtgtgccgac cggattcacg tgacgggcaa tacggtcatc gacgcgttgc tgacggtgac cgaacgattg acagtcgagc cggaactgcg cgcacgaatg cgtgccgcat ttccgttcct gagcgacgag cgccgaatga ttctcgtcac cgggcaccgg cgcgagaatt tcggctcggg cttcgaacag atctgcaatg cactggcggc gatcgcgacg cgttttcccg agtgccggat cgtctatccg atgcatccga atccgaacgt ccgcgagccg gtgacacgca tgctcggcga cattgaaaac atcatcctga tcgagcctca ggagtatctg cagttcgtct atctgatgag tcgcgcgtgg ctgatcctga ccgattcggg aggcatacag gaggaagcgc cgtcgctcgg caagccggtc ctcgtgatgc gcgacacgac ggaacgtccg gaagccgtcg cggccggcac ggtccgtctg gtcggcaccg acgcgcaccg gctggtcagc gaagtcacgc agctgtggga gtgcccggac gtataccgct cgatgagccg ggcacacaac ccatacggcg acggccacgc aagcgagcgc atcgtcgacc tgctgacggc cgccccgtcc gattcccgca cggcgcagcc ctggccggcc ggctcgtgtg aagcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat