SSGCID: Target Page

BuamA.00061.a

UDP-N-acetylglucosamine 2-epimerase

CENTER ID:	BuamA.00061.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00061.a.B1.GE39252	Full length( BuamA.00061.a )		1	405

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.3732
RefSeq:	YP_001810303.1
UniProt:	B1Z0G6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKKILLVFGT RPEAIKMAPL VRALKARRDV DARVCVTAQH REMLDQVLTL FGIKPDYDLN LMRQSQTLTD VTTGILQAIG VVFEDFHPDV VLVHGDTTTT LAVSLAAFYR YLPVGHVEAG LRSGDIWSPW PEELNRRVTD AVSSWHFAPT GQARDNLFSE GIPVGAVVMT GNTVIDALHD VKRMLDHNAA LNDTVAAHFP FLEPSRRVVL ITGHRRESFG EPFRHFCEAL SALANRYRDA QFVYPLHLNP NVREPARALL GDVPNIYLID PQEYLSFVFL MSRAHFIITD SGGIQEEGPA LGKPVLVTRE TTERPEAIQA GTARLVGTDR ERIIWEASRL FDSESAYEEM SRASNPYGDG HASERIVHAL MHMPGASANA TSFSMGAAEM PFNALALGLQ ALRSP

NT Sequence

ATGAAAAAGA TCTTGCTTGT GTTCGGGACT CGGCCGGAAG CCATCAAGAT GGCGCCGCTC GTGCGCGCAC TCAAGGCGCG CAGGGATGTC GACGCCCGGG TATGCGTCAC CGCCCAGCAT CGGGAAATGC TGGATCAGGT CCTCACGTTG TTCGGCATCA AGCCCGACTA CGATCTCAAT CTGATGCGTC AGAGCCAGAC GCTGACCGAC GTGACGACCG GCATCCTGCA GGCGATCGGC GTCGTTTTCG AGGATTTCCA TCCGGACGTC GTCCTGGTGC ACGGCGATAC GACGACGACG CTGGCCGTGA GTCTCGCCGC GTTCTATCGC TATCTGCCGG TCGGACACGT GGAAGCCGGT TTGCGCAGCG GCGACATCTG GTCGCCGTGG CCCGAGGAAC TGAATCGCCG CGTGACCGAC GCTGTGTCGT CATGGCATTT CGCACCGACC GGCCAGGCGC GCGACAACCT GTTCAGCGAA GGCATTCCGG TGGGTGCGGT CGTGATGACC GGCAACACCG TGATCGACGC GCTGCACGAC GTCAAACGGA TGCTCGATCA CAATGCCGCG CTCAACGACA CCGTCGCCGC GCACTTTCCG TTTCTCGAGC CGTCGCGTCG CGTCGTGCTG ATCACCGGCC ATCGTCGCGA GAGCTTCGGC GAACCGTTCC GGCATTTCTG CGAGGCGCTC AGCGCGCTCG CGAACCGCTA TCGCGATGCG CAGTTCGTCT ATCCGCTGCA CTTGAATCCG AACGTGCGCG AGCCGGCGCG TGCATTGCTC GGCGACGTGC CGAACATTTA CCTGATCGAT CCGCAGGAAT ATCTTTCGTT CGTATTCCTG ATGTCACGCG CGCATTTCAT CATTACCGAC TCGGGCGGCA TCCAGGAGGA AGGGCCGGCG CTCGGCAAGC CGGTGCTCGT CACGCGCGAG ACGACCGAGC GCCCCGAAGC GATCCAGGCC GGCACCGCGC GGCTCGTCGG CACGGATCGG GAACGGATCA TCTGGGAGGC GTCGCGGCTG TTCGACAGCG AGAGCGCGTA TGAGGAAATG TCGCGGGCGA GCAATCCGTA TGGCGACGGC CATGCGAGCG AGCGGATCGT CCACGCGCTG ATGCATATGC CGGGGGCGAG CGCGAACGCG ACAAGCTTTT CGATGGGCGC CGCGGAGATG CCGTTCAACG CGCTCGCGCT GGGGTTGCAG GCGTTGCGCT CGCCT

Details for BuamA.00061.a.B1.GE39252

HARVESTED ON:	6/10/2015
SEQUENCED ON:	6/5/2015
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMK KILLVFGTRP EAIKMAPLVR ALKARRDVDA RVCVTAQHRE MLDQVLTLFG IKPDYDLNLM RQSQTLTDVT TGILQAIGVV FEDFHPDVVL VHGDTTTTLA VSLAAFYRYL PVGHVEAGLR SGDIWSPWPE ELNRRVTDAV SSWHFAPTGQ ARDNLFSEGI PVGAVVMTGN TVIDALHDVK RMLDHNAALN DTVAAHFPFL EPSRRVVLIT GHRRESFGEP FRHFCEALSA LANRYRDAQF VYPLHLNPNV REPARALLGD VPNIYLIDPQ EYLSFVFLMS RAHFIITDSG GIQEEGPALG KPVLVTRETT ERPEAIQAGT ARLVGTDRER IIWEASRLFD SESAYEEMSR ASNPYGDGHA SERIVHALMH MPGASANATS FSMGAAEMPF NAL

Validated NT Sequence

cgcgagcgcg ttgaacggca tctccgcggc gcccatcgaa aagcttgtcg cgttcgcgct cgcccccggc atatgcatca gcgcgtggac gatccgctcg ctcgcatggc cgtcgccata cggattgctc gcccgcgaca tttcctcata cgcgctctcg ctgtcgaaca gccgcgacgc ctcccagatg atccgttccc gatccgtgcc gacgagccgc gcggtgccgg cctggatcgc ttcggggcgc tcggtcgtct cgcgcgtgac gagcaccggc ttgccgagcg ccggcccttc ctcctggatg ccgcccgagt cggtaatgat gaaatgcgcg cgtgacatca ggaatacgaa cgaaagatat tcctgcggat cgatcaggta aatgttcggc acgtcgccga gcaatgcacg cgccggctcg cgcacgttcg gattcaagtg cagcggatag acgaactgcg catcgcgata gcggttcgcg agcgcgctga gcgcctcgca gaaatgccgg aacggttcgc cgaagctctc gcgacgatgg ccggtgatca gcacgacgcg acgcgacggc tcgagaaacg gaaagtgcgc ggcgacggtg tcgttgagcg cggcattgtg atcgagcatc cgtttgacgt cgtgcagcgc gtcgatcacg gtgttgccgg tcatcacgac cgcacccacc ggaatgcctt cgctgaacag gttgtcgcgc gcctggccgg tcggtgcgaa atgccatgac gacacagcgt cggtcacgcg gcgattcagt tcctcgggcc acggcgacca gatgtcgccg ctgcgcaaac cggcttccac gtgtccgacc ggcagatagc gatagaacgc ggcgagactc acggccagcg tcgtcgtcgt atcgccgtgc accaggacga cgtccggatg gaaatcctcg aaaacgacgc cgatcgcctg caggatgccg gtcgtcacgt cggtcagcgt ctggctctga cgcatcagat tgagatcgta gtcgggcttg atgccgaaca acgtgaggac ctgatccagc atttcccgat gctgggcggt gacgcatacc cgggcgtcga catccctgcg cgccttgagt gcgcgcacga gcggcgccat cttgatggct tccggccgag tcccgaacac aagcaagatc tttttcatat ggtggtggtg gtggtgagcc at

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa agatcttgct tgtgttcggg actcggccgg aagccatcaa gatggcgccg ctcgtgcgcg cactcaaggc gcgcagggat gtcgacgccc gggtatgcgt caccgcccag catcgggaaa tgctggatca ggtcctcacg ttgttcggca tcaagcccga ctacgatctc aatctgatgc gtcagagcca gacgctgacc gacgtgacga ccggcatcct gcaggcgatc ggcgtcgttt tcgaggattt ccatccggac gtcgtcctgg tgcacggcga tacgacgacg acgctggccg tgagtctcgc cgcgttctat cgctatctgc cggtcggaca cgtggaagcc ggtttgcgca gcggcgacat ctggtcgccg tggcccgagg aactgaatcg ccgcgtgacc gacgctgtgt cgtcatggca tttcgcaccg accggccagg cgcgcgacaa cctgttcagc gaaggcattc cggtgggtgc ggtcgtgatg accggcaaca ccgtgatcga cgcgctgcac gacgtcaaac ggatgctcga tcacaatgcc gcgctcaacg acaccgtcgc cgcgcacttt ccgtttctcg agccgtcgcg tcgcgtcgtg ctgatcaccg gccatcgtcg cgagagcttc ggcgaaccgt tccggcattt ctgcgaggcg ctcagcgcgc tcgcgaaccg ctatcgcgat gcgcagttcg tctatccgct gcacttgaat ccgaacgtgc gcgagccggc gcgtgcattg ctcggcgacg tgccgaacat ttacctgatc gatccgcagg aatatctttc gttcgtattc ctgatgtcac gcgcgcattt catcattacc gactcgggcg gcatccagga ggaagggccg gcgctcggca agccggtgct cgtcacgcgc gagacgaccg agcgccccga agcgatccag gccggcaccg cgcggctcgt cggcacggat cgggaacgga tcatctggga ggcgtcgcgg ctgttcgaca gcgagagcgc gtatgaggaa atgtcgcggg cgagcaatcc gtatggcgac ggccatgcga gcgagcggat cgtccacgcg ctgatgcata tgccgggggc gagcgcgaac gcgacaagct tttcgatggg cgccgcggag atgccgttca acgcgctcgc gctggggttg caggcgttgc gctcgccttg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat