BuamA.00057.o

ABC transporter related

CENTER ID: BuamA.00057.o
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00057.o.A1.GE33122 Full length( BuamA.00057.o ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.5697
RefSeq: YP_001812145.1
UniProt: B1Z2G7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MASISMRRVQ KAYGEHAPVI RDVDLEIGAH EFCVFLGPSG CGKSTLLRMI AGLEDLSDGE LSIDGRRVDD VPAAERGVAM VFQSYALFPH MTVYENMAFG LKLARVPKAE IDRKVRDAAR ILQLDTLLER KPKALSGGQR QRVAIGRAIV REPGVFLFDE PLSNLDAALR GQTRIEIAKL HRQFERASVV YVTHDQTEAM TLADKIVLLH AGADTAQHGS IAQIGAPLDL YHHPASRFVA GFIGSPRMNF LPAVVAACDA RHTTVTVVPS GETFTLPRDG AALAPGATVT LGIRPEHLTL GAAPDATTLA RDVALVERLG EQTYVHLDQP GGTPLIAKVP GDAPLRIGER VHAQAPAAAC HLFTEDGRAV PARADVPVHH YA
NT Sequence
ATGGCGAGCA TCTCGATGCG ACGCGTGCAG AAAGCCTATG GCGAGCACGC GCCGGTCATT CGCGACGTCG ATCTGGAGAT CGGCGCGCAC GAGTTCTGCG TGTTCCTCGG GCCGTCCGGC TGCGGCAAGT CGACCCTGCT GCGGATGATC GCTGGCCTCG AGGATCTGAG CGACGGCGAA TTGTCGATCG ACGGACGCCG CGTCGACGAC GTGCCGGCCG CCGAGCGCGG CGTCGCGATG GTGTTCCAGA GCTACGCGCT GTTTCCGCAC ATGACGGTCT ACGAGAACAT GGCGTTCGGG CTGAAGCTCG CGCGCGTGCC GAAGGCCGAG ATCGACCGGA AGGTGCGCGA CGCCGCGCGG ATCCTGCAGC TCGATACGCT GCTCGAGCGC AAGCCGAAGG CGCTGTCCGG CGGCCAGCGG CAGCGCGTCG CGATCGGCCG CGCGATCGTG CGCGAGCCCG GCGTATTCCT GTTCGACGAA CCGCTGTCGA ACCTCGACGC CGCGCTGCGC GGCCAGACCC GCATCGAAAT CGCGAAGCTG CACCGGCAGT TCGAACGCGC GAGCGTCGTC TACGTGACGC ACGACCAGAC CGAAGCGATG ACGCTCGCCG ACAAGATCGT GCTGTTGCAC GCCGGCGCCG ACACCGCGCA GCACGGCAGC ATCGCGCAGA TCGGCGCGCC GCTCGACCTC TATCACCATC CCGCCAGCCG CTTCGTCGCC GGCTTCATCG GCTCGCCGCG GATGAACTTC CTGCCGGCCG TCGTCGCCGC CTGCGACGCA CGGCACACCA CCGTCACCGT CGTGCCGTCC GGCGAAACCT TCACGCTGCC GCGCGACGGC GCCGCGCTCG CACCGGGCGC GACCGTCACG CTCGGCATCC GCCCCGAACA CCTGACGCTC GGCGCGGCGC CCGACGCGAC GACGCTCGCA CGCGACGTCG CGCTCGTCGA ACGCCTCGGC GAGCAGACCT ACGTGCACCT CGACCAGCCC GGCGGCACGC CGCTGATCGC GAAAGTGCCC GGCGACGCGC CGCTGCGCAT CGGCGAGCGC GTGCACGCGC AGGCGCCGGC CGCGGCCTGT CACCTGTTCA CCGAAGACGG CCGCGCGGTG CCCGCGCGCG CCGACGTTCC CGTCCACCAC TACGCA
Details for BuamA.00057.o.A1.GE33122
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMASISMRRV QKAYGEHAPV IRDVDLEIGA HEFCVFLGPS GCGKSTLLRM IAGLEDLSDG ELSIDGRRVD DVPAAERGVA MVFQSYALFP HMTVYENMAF GLKLARVPKA EIDRKVRDAA RILQLDTLLE RKPKALSGGQ RQRVAIGRAI VREPGVFLFD EPLSNLDAAL RGQTRIEIAK LHRQFERASV VYVTHDQTEA MTLADKIVLL HAGADTAQHG SIAQIGAPLD LYHHPASRFV AGFIGSPRMN FLPAVVAACD ARHTTVTVVP SGETFTLPRD GAALAPGATV TLGIRPEHLT LGAAPXATTL ARXXALXERL XXXXXXXXDX PXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXXXXXXXXX XXX
Validated NT Sequence
attttgttnn ntttaagaag gagatatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcga gcatctcgat gcgacgcgtg cagaaagcct atggcgagca cgcgccggtc attcgcgacg tcgatctgga gatcggcgcg cacgagttct gcgtgttcct cgggccgtcc ggctgcggca agtcgaccct gctgcggatg atcgctggcc tcgaggatct gagcgacggc gaattgtcga tcgacggacg ccgcgtcgac gacgtgccgg ccgccgagcg cggcgtcgcg atggtgttcc agagctacgc gctgtttccg cacatgacgg tctacgagaa catggcgttc gggctgaagc tcgcgcgcgt gccgaaggcc gagatcgacc ggaaggtgcg cgacgccgcg cggatcctgc agctcgatac gctgctcgag cgcaagccga aggcgctgtc cggcggccag cggcagcgcg tcgcgatcgg ccgcgcgatc gtgcgcgagc ccggcgtatt cctgttcgac gaaccgctgt cgaacctcga cgccgcgctg cgcggccaga cccgcatcga aatcgcgaag ctgcaccggc agttcgaacg cgcgagcgtc gtctacgtga cgcacgacca gaccgaagcg atgacgctcg ccgacaagat cgtgctgttg cacgccggcg ccgacaccgc gcagcacggc agcatcgcgc agatcggcgc gccgctcgac ctctatcacc atcccgccag ccgcttcgtc gccggcttca tcggctcgcc gcggatgaac ttcctgccgg ccgtcgtcgc cgcctgcgac gcacggcaca ccaccgtcac cgtcgtgccg tccggcgaaa ccttcacgct gccgcgcgac ggcgccgcgc tcgcaccggg cgcgaccgtc acgctcggca tccgccccga acacctgacg ctcggcgcgg cgcccgangc gacgacnctc gcacgcgang ncgcgctcgn cgaacgcctc gnnnannana nnnacnngnn cntcgaccan ccnnggnnnn ncnctnnncn cnaaannnnc nggnnnnnnn nnnnnnnnnn tnnnnnnnnn nnnnnnngnn nnnggnnnnn nnnnnnnnnt nncngntnnn cnnnnaannn nnnnnnnnnn nnnnnnnnnn nnnnnnccnc cnnnannngn nnnnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMASISMRRV QKAYGEHAPV IRDVDLEIGA HEFCVFLGPS GCGKSTLLRM IAGLEDLSDG ELSIDGRRVD DVPAAERGVA MVFQSYALFP HMTVYENMAF GLKLARVPKA EIDRKVRDAA RILQLDTLLE RKPKALSGGQ RQRVAIGRAI VREPGVFLFD EPLSNLDAAL RGQTRIEIAK LHRQFERASV VYVTHDQTEA MTLADKIVLL HAGADTAQHG SIAQIGAPLD LYHHPASRFV AGFIGSPRMN FLPAVVAACD ARHTTVTVVP SGETFTLPRD GAALAPGATV TLGIRPEHLT LGAAPDATTL ARDVALVERL GEQTYVHLDQ PGGTPLIAKV PGDAPLRIGE RVHAQAPAAA CHLFTEDGRA VPARADVPVH HYA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgagcatc tcgatgcgac gcgtgcagaa agcctatggc gagcacgcgc cggtcattcg cgacgtcgat ctggagatcg gcgcgcacga gttctgcgtg ttcctcgggc cgtccggctg cggcaagtcg accctgctgc ggatgatcgc tggcctcgag gatctgagcg acggcgaatt gtcgatcgac ggacgccgcg tcgacgacgt gccggccgcc gagcgcggcg tcgcgatggt gttccagagc tacgcgctgt ttccgcacat gacggtctac gagaacatgg cgttcgggct gaagctcgcg cgcgtgccga aggccgagat cgaccggaag gtgcgcgacg ccgcgcggat cctgcagctc gatacgctgc tcgagcgcaa gccgaaggcg ctgtccggcg gccagcggca gcgcgtcgcg atcggccgcg cgatcgtgcg cgagcccggc gtattcctgt tcgacgaacc gctgtcgaac ctcgacgccg cgctgcgcgg ccagacccgc atcgaaatcg cgaagctgca ccggcagttc gaacgcgcga gcgtcgtcta cgtgacgcac gaccagaccg aagcgatgac gctcgccgac aagatcgtgc tgttgcacgc cggcgccgac accgcgcagc acggcagcat cgcgcagatc ggcgcgccgc tcgacctcta tcaccatccc gccagccgct tcgtcgccgg cttcatcggc tcgccgcgga tgaacttcct gccggccgtc gtcgccgcct gcgacgcacg gcacaccacc gtcaccgtcg tgccgtccgg cgaaaccttc acgctgccgc gcgacggcgc cgcgctcgca ccgggcgcga ccgtcacgct cggcatccgc cccgaacacc tgacgctcgg cgcggcgccc gacgcgacga cgctcgcacg cgacgtcgcg ctcgtcgaac gcctcggcga gcagacctac gtgcacctcg accagcccgg cggcacgccg ctgatcgcga aagtgcccgg cgacgcgccg ctgcgcatcg gcgagcgcgt gcacgcgcag gcgccggccg cggcctgtca cctgttcacc gaagacggcc gcgcggtgcc cgcgcgcgcc gacgttcccg tccaccacta cgcataaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa