SSGCID: Target Page

BuamA.00057.m

ABC transporter related

CENTER ID:	BuamA.00057.m
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00057.m.A1.GE33120	Full length( BuamA.00057.m )		1	257

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.5134
RefSeq:	YP_001811607.1
UniProt:	B1YZJ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSANAELLKV AGLQMRFGGL LAVDGIDFDV RRNEVFAIIG PNGAGKTTVF NCVGGFYKPT GGDVVLDGHA IGGLPSHKIA LKGLVRTFQN IRLFKSLTVV ENLLVAQHRK VKAGLLPGLF STPAYRRAEK EALERAAVWL DRMGLRSVAN RAAGTLSYGH QRRLEIARCM ITEPRLLMLD EPAAGLNPQE KIELQHLIDK LRHEFGVSVL LIEHDMSLVM GVSDRILVME HGRPIVIGTP EAVRNDPRVI KAYLGEE

NT Sequence

ATGAGCGCGA ATGCAGAACT GTTGAAGGTC GCCGGGCTGC AGATGCGCTT CGGCGGGTTG CTCGCGGTGG ACGGCATCGA TTTCGACGTG CGCCGTAACG AGGTGTTCGC GATCATCGGG CCGAACGGTG CGGGCAAGAC CACCGTGTTC AACTGCGTCG GCGGCTTCTA CAAGCCGACC GGCGGCGACG TCGTGCTCGA CGGCCACGCG ATCGGCGGGC TGCCGAGCCA CAAGATCGCG TTGAAGGGCC TGGTGCGCAC GTTCCAGAAC ATCCGCCTGT TCAAGTCGCT GACGGTCGTC GAGAACCTGC TCGTCGCGCA GCATCGCAAG GTGAAGGCCG GGCTGCTGCC GGGCCTGTTC TCGACGCCCG CGTATCGTCG CGCCGAGAAG GAAGCGCTCG AACGTGCGGC CGTGTGGCTC GACCGGATGG GGCTGAGGTC GGTCGCCAAC CGGGCGGCCG GCACGCTGTC GTACGGGCAC CAGCGGCGTC TGGAGATCGC GCGCTGCATG ATCACCGAGC CGCGTCTGCT GATGCTCGAC GAGCCGGCGG CCGGCCTCAA CCCGCAGGAA AAGATCGAGC TGCAGCACCT GATCGACAAG CTGCGCCATG AGTTCGGCGT GTCGGTGCTG CTGATCGAAC ACGACATGAG CCTCGTGATG GGCGTGTCGG ACCGCATCCT CGTGATGGAG CACGGCCGGC CGATCGTGAT CGGCACGCCG GAAGCGGTCC GCAACGACCC GCGCGTGATC AAGGCGTATC TGGGGGAAGA G

Details for BuamA.00057.m.A1.GE33120

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMSANAELLK VAGLQMRFGG LLAVDGIDFD VRRNEVFAII GPNGAGKTTV FNCVGGFYKP TGGDVVLDGH AIGGLPSHKI ALKGLVRTFQ NIRLFKSLTV VENLLVAQHR KVKAGLLPGL FSTPAYRRAE KEALERAAVW LDRMGLRSVA NRAAGTLSYG HQRRLEIARC MITEPRLLML DEPAAGLNPQ EKIELQHLID KLRHEFGVSV LLIEHDMSLV MGVSXRILVM EHGRPIVIGT PEXVRNDPRV IKAYLGEE

Validated NT Sequence

aattttnntn nntttaagaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgagc gcgaatgcag aactgttgaa ggtcgccggg ctgcagatgc gcttcggcgg gttgctcgcg gtggacggca tcgatttcga cgtgcgccgt aacgaggtgt tcgcgatcat cgggccgaac ggtgcgggca agaccaccgt gttcaactgc gtcggcggct tctacaagcc gaccggcggc gacgtcgtgc tcgacggcca cgcgatcggc gggctgccga gccacaagat cgcgttgaag ggcctggtgc gcacgttcca gaacatccgc ctgttcaagt cgctgacggt cgtcgagaac ctgctcgtcg cgcagcatcg caaggtgaag gccgggctgc tgccgggcct gttctcgacg cccgcgtatc gtcgcgccga gaaggaagcg ctcgaacgtg cggccgtgtg gctcgaccgg atggggctga ggtcggtcgc caaccgggcg gccggcacgc tgtcgtacgg gcaccagcgg cgtctggaga tcgcgcgctg catgatcacc gagccgcgtc tgctgatgct cgacgagccg gcggccggcc tcaacccgca ggaaaagatc gagctgcagc acctgatcga caagctgcgc catgagttcg gcgtgtcggt gctgctgatc gaacacgaca tgagcctcgt gatgggcgtg tcggancgca tcctcgtgat ggagcacggc cggccgatcg tgatcggcac gccngaancg gtccgcaacg acccgcgcgt gatcaaggcg tatctggggg aagagtaata aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaanct ganttgntnn gnnnnngnnn nnnncgctga ncnnncnnng ncgnnnnnnn anncncnntn nnnnnggnnn nntnnnnnnn gnnnnnnnnn nnnnnngcnn nnnnnnnang cngcnnnnnn nnnncnnnnn ncnnnnccnn nnnannnnnn ngnnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcgaat gcagaactgt tgaaggtcgc cgggctgcag atgcgcttcg gcgggttgct cgcggtggac ggcatcgatt tcgacgtgcg ccgtaacgag gtgttcgcga tcatcgggcc gaacggtgcg ggcaagacca ccgtgttcaa ctgcgtcggc ggcttctaca agccgaccgg cggcgacgtc gtgctcgacg gccacgcgat cggcgggctg ccgagccaca agatcgcgtt gaagggcctg gtgcgcacgt tccagaacat ccgcctgttc aagtcgctga cggtcgtcga gaacctgctc gtcgcgcagc atcgcaaggt gaaggccggg ctgctgccgg gcctgttctc gacgcccgcg tatcgtcgcg ccgagaagga agcgctcgaa cgtgcggccg tgtggctcga ccggatgggg ctgaggtcgg tcgccaaccg ggcggccggc acgctgtcgt acgggcacca gcggcgtctg gagatcgcgc gctgcatgat caccgagccg cgtctgctga tgctcgacga gccggcggcc ggcctcaacc cgcaggaaaa gatcgagctg cagcacctga tcgacaagct gcgccatgag ttcggcgtgt cggtgctgct gatcgaacac gacatgagcc tcgtgatggg cgtgtcggac cgcatcctcg tgatggagca cggccggccg atcgtgatcg gcacgccgga agcggtccgc aacgacccgc gcgtgatcaa ggcgtatctg ggggaagagt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa