SSGCID: Target Page

BuamA.00057.l

ABC transporter related

CENTER ID:	BuamA.00057.l
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00057.l.A1.GE33119	Full length( BuamA.00057.l )		1	233

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.5133
RefSeq:	YP_001811606.1
UniProt:	B1YZJ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLKLEQVHTH YGAVEALAGV SIEVKKGEIV TLIGSNGAGK TTLMMTVCGT PRASSGRVLF EGNDITAMST HQIMRQGMAI SPEGRRVFPS LTVLENLKMG GFFASRHEID DGIEHVFKLF PRLKERATQR AGTMSGGEQQ MLAIGRALMS KPRLLLLDEP TLGLAPLVIA QIFDIIRTIR DEGVTVFLVE QNANKALGVA DRGYVLETGR VVLADTGANL LANDRIKQAY LGG

NT Sequence

ATGCTGAAGC TGGAACAGGT CCATACGCAC TACGGCGCGG TCGAGGCGCT CGCGGGCGTG TCGATCGAGG TGAAGAAGGG CGAGATCGTC ACGCTGATCG GCAGCAACGG CGCCGGCAAG ACGACGCTGA TGATGACCGT GTGCGGCACG CCGCGCGCAT CGTCGGGCCG CGTACTGTTC GAGGGCAACG ACATCACCGC GATGTCGACG CACCAGATCA TGCGGCAGGG GATGGCGATC TCGCCGGAAG GGCGGCGCGT GTTCCCGAGC CTGACGGTGC TCGAGAACCT GAAGATGGGC GGCTTCTTCG CGAGCCGTCA CGAGATCGAC GACGGCATCG AGCACGTGTT CAAGCTGTTT CCGCGCCTGA AGGAGCGCGC GACGCAGCGG GCCGGCACGA TGTCGGGCGG CGAGCAGCAG ATGCTGGCGA TCGGCCGCGC GCTGATGAGC AAGCCGCGCT TGCTGCTGCT CGACGAGCCG ACGCTTGGCC TCGCGCCGCT CGTGATCGCG CAGATCTTCG ACATCATCCG GACGATCCGC GACGAAGGTG TCACGGTGTT CCTGGTCGAA CAGAACGCGA ACAAGGCGCT CGGTGTGGCC GATCGCGGCT ACGTGCTCGA AACGGGGCGC GTGGTGCTGG CCGACACGGG CGCGAACCTG CTCGCGAACG ACCGGATCAA GCAGGCGTAT CTCGGCGGT

Details for BuamA.00057.l.A1.GE33119

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	98
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMLKLEQVHT HYGAVEALAG VSIEVKKGEI VTLIGSNGAG KTTLMMTVCG TPRASSGRVL FEGNDITAMS THQIMRQGMA ISPEGRRVFP SLTVLENLKM GGFFASRHEI DDGIEHVFKL FPRLKERATQ RAGTMSGGEQ QMLAIGRALM SKPRLLLLDE PTLGLAPLVI AQIFDIIRTI RDXXVTVFLV EQNANXALGV ADRGYVLETG RVVLADTGAN LLANDRIKQA YLGG

Validated NT Sequence

ttttnnttnn ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgctga agctggaaca ggtccatacg cactacggcg cggtcgaggc gctcgcgggc gtgtcgatcg aggtgaagaa gggcgagatc gtcacgctga tcggcagcaa cggcgccggc aagacgacgc tgatgatgac cgtgtgcggc acgccgcgcg catcgtcggg ccgcgtactg ttcgagggca acgacatcac cgcgatgtcg acgcaccaga tcatgcggca ggggatggcg atctcgccgg aagggcggcg cgtgttcccg agcctgacgg tgctcgagaa cctgaagatg ggcggcttct tcgcgagccg tcacgagatc gacgacggca tcgagcacgt gttcaagctg tttccgcgcc tgaaggagcg cgcgacgcag cgggccggca cgatgtcggg cggcgagcag cagatgctgg cgatcggccg cgcgctgatg agcaagccgc gcttgctgct gctcgacgag ccgacgcttg gcctcgcgcc gctcgtgatc gcgcagatct tcgacatcat ccggacgatc cgcgacgann ntgtcacggt gttcctggtc gaacagaacg cgaacaangc gctcggtgtg gccgatcgcg gctacgtgct cgaaacgggg cgcgtggtgc tggccgacac gggcgcgaac ctgctcgcga acgaccggat caagcaggcg tatctcggcg gttaataaac agcacgaaca agttctgcag ccaagcttct cgaggatncc ggctgctaac aaagcccgaa aggnagctga nttggctgct gnnnnnnnnn naaaaaaaaa aaaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMLKLEQVHT HYGAVEALAG VSIEVKKGEI VTLIGSNGAG KTTLMMTVCG TPRASSGRVL FEGNDITAMS THQIMRQGMA ISPEGRRVFP SLTVLENLKM GGFFASRHEI DDGIEHVFKL FPRLKERATQ RAGTMSGGEQ QMLAIGRALM SKPRLLLLDE PTLGLAPLVI AQIFDIIRTI RDEGVTVFLV EQNANKALGV ADRGYVLETG RVVLADTGAN LLANDRIKQA YLGG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctgaagctg gaacaggtcc atacgcacta cggcgcggtc gaggcgctcg cgggcgtgtc gatcgaggtg aagaagggcg agatcgtcac gctgatcggc agcaacggcg ccggcaagac gacgctgatg atgaccgtgt gcggcacgcc gcgcgcatcg tcgggccgcg tactgttcga gggcaacgac atcaccgcga tgtcgacgca ccagatcatg cggcagggga tggcgatctc gccggaaggg cggcgcgtgt tcccgagcct gacggtgctc gagaacctga agatgggcgg cttcttcgcg agccgtcacg agatcgacga cggcatcgag cacgtgttca agctgtttcc gcgcctgaag gagcgcgcga cgcagcgggc cggcacgatg tcgggcggcg agcagcagat gctggcgatc ggccgcgcgc tgatgagcaa gccgcgcttg ctgctgctcg acgagccgac gcttggcctc gcgccgctcg tgatcgcgca gatcttcgac atcatccgga cgatccgcga cgaaggtgtc acggtgttcc tggtcgaaca gaacgcgaac aaggcgctcg gtgtggccga tcgcggctac gtgctcgaaa cggggcgcgt ggtgctggcc gacacgggcg cgaacctgct cgcgaacgac cggatcaagc aggcgtatct cggcggttaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa