BuamA.00057.i

Urea ABC transporter, ATP-binding protein UrtD

CENTER ID: BuamA.00057.i
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00057.i.A1.GE33116 Full length( BuamA.00057.i ) 1 285
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.813
RefSeq: YP_001807507.1
UniProt: B1YUG4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNGTALYQFT PPAEDELLTV SGTASMGRVV PPGIDTSHGT ILYLEDIEVS FDGFRALNKL SLAIDAGELR CVIGPNGAGK TTMMDVITGK TRADAGKVFL GQTIDLARMN EPEIARAGIG RKFQKPTVFE QHPVWENLEL AMQTDKGWLA SLRARLDRTA QAKIEETLAL IGLESDAYRA AGELSHGQKQ RLEIGMLLMQ RPALLLLDEP AAGMTDHETM ELAALLNTLR GTCSMMVVEH DMDFVAALAG DTGRVTVMAN GAVLAHGTLD QVKRDDAVIE SYLGR
NT Sequence
ATGAACGGCA CGGCCCTCTA CCAGTTCACG CCACCGGCCG AAGACGAGCT GCTGACCGTC AGCGGCACCG CATCGATGGG CCGCGTGGTG CCGCCCGGCA TCGACACGTC GCACGGCACG ATCCTCTACC TCGAGGACAT CGAGGTGAGC TTCGACGGCT TTCGCGCGCT GAACAAGCTG TCGCTCGCGA TCGACGCCGG GGAGCTGCGC TGCGTGATCG GCCCGAACGG CGCGGGCAAG ACCACGATGA TGGACGTGAT CACCGGCAAG ACGCGCGCCG ATGCGGGCAA GGTGTTTCTC GGCCAGACGA TCGATCTCGC GCGGATGAAC GAGCCGGAAA TCGCGCGCGC GGGCATCGGC CGCAAGTTCC AGAAGCCGAC CGTGTTCGAA CAGCATCCGG TGTGGGAAAA CCTCGAACTC GCGATGCAGA CCGACAAGGG CTGGCTCGCG TCGCTGCGCG CGCGGCTCGA CCGCACCGCG CAGGCGAAGA TCGAAGAAAC GCTCGCGCTG ATCGGCCTGG AAAGCGATGC GTATCGCGCG GCCGGCGAGC TGTCGCACGG GCAGAAGCAG CGGCTCGAGA TCGGCATGCT GCTGATGCAG CGCCCCGCCC TCCTGCTGCT CGACGAACCG GCGGCCGGGA TGACCGACCA CGAGACGATG GAACTCGCCG CGCTGCTGAA CACGCTGCGC GGCACCTGCT CGATGATGGT CGTCGAGCAT GACATGGACT TCGTCGCGGC GCTCGCGGGC GACACGGGCC GCGTGACGGT GATGGCCAAC GGCGCGGTGC TCGCGCACGG CACGCTCGAT CAGGTCAAGC GCGACGACGC GGTGATCGAG TCCTACCTCG GAAGA
Details for BuamA.00057.i.A1.GE33116
HARVESTED ON: 8/19/2011
SEQUENCED ON: 8/31/2011
EXPECTED MW: 33kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 111
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMNGTALYQF TPPAEDELLT VSGTASMGRV VPPGIDTSHG TILYLEDIEV SFDGFRALNK LSLAIDAGEL RCVIGPNGAG KTTMMDVITG KTRADAGKVF LGQTIDLARM NEPEIARAGI GRKFQKPTVF EQHPVWENLE LAMQTDKGWL ASLRARLDRT AQAKIEETLA LIGLESDAYR AAGELSHGQK QRLEIGMLLM QRPALLLLDE PAAGMTDHET MELAALLNTL RGTCSMMVVE HDMDFVAALA GDTGRVTVMA NGAVLAHGTL DXXKRDDXVI ESYLGXINST NKFCSXLLED PAANKARKXX XXXXXXXXXK
Validated NT Sequence
attttgttta nctttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatgaac ggcacggccc tctaccagtt cacgccaccg gccgaagacg agctgctgac cgtcagcggc accgcatcga tgggccgcgt ggtgccgccc ggcatcgaca cgtcgcacgg cacgatcctc tacctcgagg acatcgaggt gagcttcgac ggctttcgcg cgctgaacaa gctgtcgctc gcgatcgacg ccggggagct gcgctgcgtg atcggcccga acggcgcggg caagaccacg atgatggacg tgatcaccgg caagacgcgc gccgatgcgg gcaaggtgtt tctcggccag acgatcgatc tcgcgcggat gaacgagccg gaaatcgcgc gcgcgggcat cggccgcaag ttccagaagc cgaccgtgtt cgaacagcat ccggtgtggg aaaacctcga actcgcgatg cagaccgaca agggctggct cgcgtcgctg cgcgcgcggc tcgaccgcac cgcgcaggcg aagatcgaag aaacgctcgc gctgatcggc ctggaaagcg atgcgtatcg cgcggccggc gagctgtcgc acgggcagaa gcagcggctc gagatcggca tgctgctgat gcagcgcccc gccctcctgc tgctcgacga accggcggcc gggatgaccg accacgagac gatggaactc gccgcgctgc tgaacacgct gcgcggcacc tgctcgatga tggtcgtcga gcatgacatg gacttcgtcg cggcgctcgc gggcgacacg ggccgcgtga cggtgatggc caacggcgcg gtgctcgcgc acggcacgct cgatcngntc aagcgcgacg acncggtgat cgagtcctac ctcgganana taaacagcac gaacaagttc tgcagccanc ttctcgagga tccngctgct aacaaagccc gaaaggnnnc tgantngntg cnnnnnnnnn nnnnnnnnaa aa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMNGTALYQF TPPAEDELLT VSGTASMGRV VPPGIDTSHG TILYLEDIEV SFDGFRALNK LSLAIDAGEL RCVIGPNGAG KTTMMDVITG KTRADAGKVF LGQTIDLARM NEPEIARAGI GRKFQKPTVF EQHPVWENLE LAMQTDKGWL ASLRARLDRT AQAKIEETLA LIGLESDAYR AAGELSHGQK QRLEIGMLLM QRPALLLLDE PAAGMTDHET MELAALLNTL RGTCSMMVVE HDMDFVAALA GDTGRVTVMA NGAVLAHGTL DQVKRDDAVI ESYLGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacggcacg gccctctacc agttcacgcc accggccgaa gacgagctgc tgaccgtcag cggcaccgca tcgatgggcc gcgtggtgcc gcccggcatc gacacgtcgc acggcacgat cctctacctc gaggacatcg aggtgagctt cgacggcttt cgcgcgctga acaagctgtc gctcgcgatc gacgccgggg agctgcgctg cgtgatcggc ccgaacggcg cgggcaagac cacgatgatg gacgtgatca ccggcaagac gcgcgccgat gcgggcaagg tgtttctcgg ccagacgatc gatctcgcgc ggatgaacga gccggaaatc gcgcgcgcgg gcatcggccg caagttccag aagccgaccg tgttcgaaca gcatccggtg tgggaaaacc tcgaactcgc gatgcagacc gacaagggct ggctcgcgtc gctgcgcgcg cggctcgacc gcaccgcgca ggcgaagatc gaagaaacgc tcgcgctgat cggcctggaa agcgatgcgt atcgcgcggc cggcgagctg tcgcacgggc agaagcagcg gctcgagatc ggcatgctgc tgatgcagcg ccccgccctc ctgctgctcg acgaaccggc ggccgggatg accgaccacg agacgatgga actcgccgcg ctgctgaaca cgctgcgcgg cacctgctcg atgatggtcg tcgagcatga catggacttc gtcgcggcgc tcgcgggcga cacgggccgc gtgacggtga tggccaacgg cgcggtgctc gcgcacggca cgctcgatca ggtcaagcgc gacgacgcgg tgatcgagtc ctacctcgga agataaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa