SSGCID: Target Page

BuamA.00057.g

ABC transporter related

CENTER ID:	BuamA.00057.g
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00057.g.A1.GE33114	Full length( BuamA.00057.g )		1	252

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.88
RefSeq:	YP_001806811.1
UniProt:	B1YQK1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTADTTMPIL EVRGLAVRYG KVEALHGAAI KVGAGQIVSV IGPNGAGKST MLNAIMGALP VTGHASGTVM YRGHDVGALP VEQRVARGMC LVPEKRELFS TMTVEDNLVL GAYRRKLAGE SNFLDQLDHV FALFPRLKER RKQAAGTLSG GERQMLAVGR ALMGKPDLLM LDEPSLGLAP LIVKEIFHII SALRGTGVAT LLIEQNARAA LQISDYGYVL ETGELALEGP AAELAQNPRV IETYLGLAKK TA

NT Sequence

ATGACGGCGG ACACGACGAT GCCGATTCTC GAGGTGCGCG GACTGGCGGT CCGATACGGG AAAGTCGAGG CGCTGCACGG TGCGGCGATC AAGGTGGGCG CCGGGCAGAT CGTCAGCGTC ATCGGCCCGA ACGGTGCCGG CAAGTCGACG ATGCTGAACG CGATCATGGG TGCGTTGCCC GTGACCGGGC ATGCGTCGGG CACGGTCATG TATCGCGGCC ACGACGTGGG CGCGCTACCG GTCGAGCAGC GCGTCGCGCG CGGGATGTGC CTCGTGCCGG AAAAGCGCGA GCTGTTCAGC ACGATGACGG TCGAGGACAA CCTCGTGCTG GGCGCGTATC GCCGCAAGCT CGCGGGCGAG TCCAACTTTC TCGACCAGCT CGATCATGTG TTTGCGCTGT TTCCGCGGCT CAAGGAGCGG CGCAAGCAGG CGGCCGGTAC GCTGTCGGGC GGCGAGCGGC AGATGCTTGC GGTGGGCCGC GCGCTGATGG GCAAACCCGA CCTGCTGATG CTCGATGAGC CGAGCCTCGG GTTGGCACCG CTGATCGTGA AGGAAATTTT TCATATCATC AGCGCGTTGC GCGGCACGGG TGTGGCGACG CTGCTGATCG AGCAGAACGC GCGGGCGGCA CTGCAGATCT CGGACTACGG TTATGTACTG GAGACGGGGG AGCTAGCGCT GGAAGGGCCG GCGGCCGAGC TGGCACAAAA TCCGCGCGTG ATCGAAACTT ACCTGGGGTT GGCGAAGAAA ACGGCT

Details for BuamA.00057.g.A1.GE33114

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMTADTTMPI LEVRGLAVRY GKVEALHGAA IKVGAGQIVS VIGPNGAGKS TMLNAIMGAL PVTGHASGTV MYRGHDVGAL PVEQRVARGM CLVPEKRELF STMTVEDNLV LGAYRRKLAG ESNFLDQLDH VFALFPRLKE RRKQAAGTLS GGERQMLAVG RALMGKPDLL MLDEPSLGLA PLIVKEIFHI ISALRGTGVA TLLIEQNARA ALQISDYGYV LETGELALEG PAAELAQNPR VIETYLGLAK KTA

Validated NT Sequence

annnnnttgt ttanctttaa ganggagata taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg acggcggaca cgacgatgcc gattctcgag gtgcgcggac tggcggtccg atacgggaaa gtcgaggcgc tgcacggtgc ggcgatcaag gtgggcgccg ggcagatcgt cagcgtcatc ggcccgaacg gtgccggcaa gtcgacgatg ctgaacgcga tcatgggtgc gttgcccgtg accgggcatg cgtcgggcac ggtcatgtat cgcggccacg acgtgggcgc gctaccggtc gagcagcgcg tcgcgcgcgg gatgtgcctc gtgccggaaa agcgcgagct gttcagcacg atgacggtcg aggacaacct cgtgctgggc gcgtatcgcc gcaagctcgc gggcgagtcc aactttctcg accagctcga tcatgtgttt gcgctgtttc cgcggctcaa ggagcggcgc aagcaggcgg ccggtacgct gtcgggcggc gagcggcaga tgcttgcggt gggccgcgcg ctgatgggca aacccgacct gctgatgctc gatgagccga gcctcgggtt ggcaccgctg atcgtgaagg aaatttttca tatcatcagc gcgttgcgcg gcacgggtgt ggcgacgctg ctgatcgagc agaacgcgcg ggcggcactg cagatctcgg actacggtta tgtactggag acgggggagc tagcgctgga agggccggcg gccgagctgg cacaaaatcc gcgcgtgatc gaaacttacc tggggttggc gaagaaaacg gcttaataaa cagcacgnan nnnttnnnna nncagcttnn ncgaggatcc ngctgctaac aaagcccgaa aggaagctna nttgnnnnnn nnnnnnnnnn naaaaaaaaa aaaaaaaann nnnnnnnnnn gncnncnnnn gnnngtnnnn gnnnnnnnnn nnnngnntgn ntcgnncnnn nnnnnnntnn tncnnngnnn nnnnnnnnnn ncnnnnnnng cnnncntnnn nnnnnnncnt nncnnnnnnn nnnnncnnan nnnnnncnnn nnnnncnntn nnnnnnnnnn nngnnnnnan nnnnnnnnnt nnnnnnnnnn nnnnnnnnnn nnnc

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacggcggac acgacgatgc cgattctcga ggtgcgcgga ctggcggtcc gatacgggaa agtcgaggcg ctgcacggtg cggcgatcaa ggtgggcgcc gggcagatcg tcagcgtcat cggcccgaac ggtgccggca agtcgacgat gctgaacgcg atcatgggtg cgttgcccgt gaccgggcat gcgtcgggca cggtcatgta tcgcggccac gacgtgggcg cgctaccggt cgagcagcgc gtcgcgcgcg ggatgtgcct cgtgccggaa aagcgcgagc tgttcagcac gatgacggtc gaggacaacc tcgtgctggg cgcgtatcgc cgcaagctcg cgggcgagtc caactttctc gaccagctcg atcatgtgtt tgcgctgttt ccgcggctca aggagcggcg caagcaggcg gccggtacgc tgtcgggcgg cgagcggcag atgcttgcgg tgggccgcgc gctgatgggc aaacccgacc tgctgatgct cgatgagccg agcctcgggt tggcaccgct gatcgtgaag gaaatttttc atatcatcag cgcgttgcgc ggcacgggtg tggcgacgct gctgatcgag cagaacgcgc gggcggcact gcagatctcg gactacggtt atgtactgga gacgggggag ctagcgctgg aagggccggc ggccgagctg gcacaaaatc cgcgcgtgat cgaaacttac ctggggttgg cgaagaaaac ggcttaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa