SSGCID: Target Page

BuamA.00057.a

ABC transporter related

CENTER ID:	BuamA.00057.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00057.a.A1.GE33108	Full length( BuamA.00057.a )		1	258

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00057.a.A1.PS01419	Full length( BuamA.00057.a )		1	258

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.2791
RefSeq:	YP_001809401.1
UniProt:	B1YN40

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNALPNRQPA GETSSLVVRN LKKRYGSRTV VKDVSLDVKS GEVVGLLGPN GAGKTTSFYM IVGLVPLDAG EISLNGSSIS LLPIHKRASL GLSYLPQEAS VFRKLTVEEN IRAVLELQHG DDGKRLSKDA IAARTEELLD ELQIGHLREN PALSLSGGER RRVEIARALA TSPNFILLDE PFAGVDPIAV LEIQKIVKFL KQRNIGVLIT DHNVRETLGI CDHAYIISDG SVLAAGAPSE IIENESVRRV YLGEHFRM

NT Sequence

ATGAACGCGC TACCGAATCG CCAGCCGGCC GGCGAGACGA GCTCGCTCGT CGTGCGCAAC CTGAAGAAAC GCTACGGCTC GCGCACGGTC GTCAAGGACG TGTCGCTCGA CGTGAAGAGC GGCGAAGTCG TCGGCCTGCT CGGCCCGAAC GGCGCGGGCA AGACGACGTC GTTCTACATG ATCGTCGGCC TCGTGCCGCT CGACGCGGGC GAGATCTCGC TGAACGGCAG CTCGATCAGC CTGCTGCCGA TCCACAAGCG TGCATCGCTC GGCCTGTCGT ACCTGCCGCA GGAAGCGTCG GTGTTCCGCA AGCTGACCGT CGAGGAAAAC ATCCGCGCGG TGCTCGAGCT GCAGCACGGC GACGACGGCA AGCGCCTGTC GAAGGACGCG ATCGCCGCGC GCACCGAGGA GCTGCTCGAC GAGCTGCAGA TCGGCCACCT GCGCGAGAAC CCCGCGCTGT CGCTGTCGGG CGGCGAACGC CGTCGCGTCG AGATCGCGCG TGCGCTTGCG ACCAGCCCGA ACTTCATCCT GCTCGACGAA CCGTTCGCCG GCGTCGATCC GATCGCGGTG CTCGAGATCC AGAAGATCGT CAAGTTCCTG AAGCAGCGCA ACATCGGCGT GTTGATCACC GATCACAACG TCCGCGAAAC GCTCGGCATC TGCGACCACG CGTACATCAT CAGCGACGGT TCGGTGCTCG CCGCCGGCGC GCCCAGCGAA ATCATCGAAA ACGAAAGCGT GCGCCGCGTG TACCTCGGCG AACACTTCCG CATG

Details for BuamA.00057.a.A1.GE33108

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	30kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MGTLEAQTQG PGSMNALPNR QPAGETSSLV VRNLKKRYGS RTVVKDVSLD VKSGEVVGLL GPNGAGKTTS FYMIVGLVPL DAGEISLNGS SISLLPIHKR ASLGLSYLPQ EASVFRKLTV EENIRAVLEL QHGDDGKRLS KDAIAARTEE LLDELQIGHL RENPALSLSG GERRRVEIAR ALATSPNFIL LDEPFAGVDP IAVLEIQKIV KFLKQRNIGV LITDHNVRET LGICDHAYII SDGSVLAAGA PSEIIENESV RRVYLGEHFR M

Validated NT Sequence

ttattacatg cggaagtgtt cgccgaggta cacgcggcgc acgctttcgt tttcgatgat ttcgctgggc gcgccggcgg cgagcaccga accgtcgctg atgatgtacg cgtggtcgca gatgccgagc gtttcgcgga cgttgtgatc ggtgatcaac acgccgatgt tgcgctgctt caggaacttg acgatcttct ggatctcgag caccgcgatc ggatcgacgc cggcgaacgg ttcgtcgagc aggatgaagt tcgggctggt cgcaagcgca cgcgcgatct cgacgcgacg gcgttcgccg cccgacagcg acagcgcggg gttctcgcgc aggtggccga tctgcagctc gtcgagcagc tcctcggtgc gcgcggcgat cgcgtccttc gacaggcgct tgccgtcgtc gccgtgctgc agctcgagca ccgcgcggat gttttcctcg acggtcagct tgcggaacac cgacgcttcc tgcggcaggt acgacaggcc gagcgatgca cgcttgtgga tcggcagcag gctgatcgag ctgccgttca gcgagatctc gcccgcgtcg agcggcacga ggccgacgat catgtagaac gacgtcgtct tgcccgcgcc gttcgggccg agcaggccga cgacttcgcc gctcttcacg tcgagcgaca cgtccttgac gaccgtgcgc gagccgtagc gtttcttcag gttgcgcacg acgagcgagc tcgtctcgcc ggccggctgg cgattcggta gcgcgttcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMNALPNRQP AGETSSLVVR NLKKRYGSRT VVKDVSLDVK SGEVVGLLGP NGAGKTTSFY MIVGLVPLDA GEISLNGSSI SLLPIHKRAS LGLSYLPQEA SVFRKLTVEE NIRAVLELQH GDDGKRLSKD AIAARTEELL DELQIGHLRE NPALSLSGGE RRRVEIARAL ATSPNFILLD EPFAGVDPIA VLEIQKIVKF LKQRNIGVLI TDHNVRETLG ICDHAYIISD GSVLAAGAPS EIIENESVRR VYLGEHFRM

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaacgcgcta ccgaatcgcc agccggccgg cgagacgagc tcgctcgtcg tgcgcaacct gaagaaacgc tacggctcgc gcacggtcgt caaggacgtg tcgctcgacg tgaagagcgg cgaagtcgtc ggcctgctcg gcccgaacgg cgcgggcaag acgacgtcgt tctacatgat cgtcggcctc gtgccgctcg acgcgggcga gatctcgctg aacggcagct cgatcagcct gctgccgatc cacaagcgtg catcgctcgg cctgtcgtac ctgccgcagg aagcgtcggt gttccgcaag ctgaccgtcg aggaaaacat ccgcgcggtg ctcgagctgc agcacggcga cgacggcaag cgcctgtcga aggacgcgat cgccgcgcgc accgaggagc tgctcgacga gctgcagatc ggccacctgc gcgagaaccc cgcgctgtcg ctgtcgggcg gcgaacgccg tcgcgtcgag atcgcgcgtg cgcttgcgac cagcccgaac ttcatcctgc tcgacgaacc gttcgccggc gtcgatccga tcgcggtgct cgagatccag aagatcgtca agttcctgaa gcagcgcaac atcggcgtgt tgatcaccga tcacaacgtc cgcgaaacgc tcggcatctg cgaccacgcg tacatcatca gcgacggttc ggtgctcgcc gccggcgcgc ccagcgaaat catcgaaaac gaaagcgtgc gccgcgtgta cctcggcgaa cacttccgca tgtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa

Details for BuamA.00057.a.A1.PS01419

PURIFICATION DATe:	3/10/2012
CONCENTRATION:	46.79mg/ml
OBSERVED MW:	30kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GAACGCGCTA CCGAATCGCC AGCCGGCCGG CGAGACGAGC TCGCTCGTCG TGCGCAACCT GAAGAAACGC TACGGCTCGC GCACGGTCGT CAAGGACGTG TCGCTCGACG TGAAGAGCGG CGAAGTCGTC GGCCTGCTCG GCCCGAACGG CGCGGGCAAG ACGACGTCGT TCTACATGAT CGTCGGCCTC GTGCCGCTCG ACGCGGGCGA GATCTCGCTG AACGGCAGCT CGATCAGCCT GCTGCCGATC CACAAGCGTG CATCGCTCGG CCTGTCGTAC CTGCCGCAGG AAGCGTCGGT GTTCCGCAAG CTGACCGTCG AGGAAAACAT CCGCGCGGTG CTCGAGCTGC AGCACGGCGA CGACGGCAAG CGCCTGTCGA AGGACGCGAT CGCCGCGCGC ACCGAGGAGC TGCTCGACGA GCTGCAGATC GGCCACCTGC GCGAGAACCC CGCGCTGTCG CTGTCGGGCG GCGAACGCCG TCGCGTCGAG ATCGCGCGTG CGCTTGCGAC CAGCCCGAAC TTCATCCTGC TCGACGAACC GTTCGCCGGC GTCGATCCGA TCGCGGTGCT CGAGATCCAG AAGATCGTCA AGTTCCTGAA GCAGCGCAAC ATCGGCGTGT TGATCACCGA TCACAACGTC CGCGAAACGC TCGGCATCTG CGACCACGCG TACATCATCA GCGACGGTTC GGTGCTCGCC GCCGGCGCGC CCAGCGAAAT CATCGAAAAC GAAAGCGTGC GCCGCGTGTA CCTCGGCGAA CACTTCCGCA TGTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA