BuamA.00046.b

Alpha,alpha-trehalose-phosphate synthase (UDP-forming)

CENTER ID: BuamA.00046.b
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00046.b.B1.GE39247 Full length( BuamA.00046.b ) 1 459
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.1059
RefSeq: YP_001807747.1
UniProt: B1YVQ0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRLIIVSNR VAPISEGEPA AGGLAIGVYD ALKETGGMWF GWSGEVVASG APQIRIEERG PVTFATVGLS RRDYDQYYRG FSNATLWPAF HYRADLIQYD RHEFDGYRRV NVWLAQQLVP LLQDDDVIWV HDYHLIPFAR ALREAGVKNR IGFFLHIPFP AAQVLVNVPP HRELVESLCA FDLLGFQTEP DLRAFCDYIE FEAGGEVARG GHTVRVNAFG NTLRAAAYPI GVYPDEIASL AQAAENGKAV RTLATSLRGR QLIMSVDRLD YSKGLVERFR AFEKLLEHQT SIRNRVSFLQ IAPSTRADLR AYQDIRLQLE AESGRINGRY AELDWAPILY IHRQYDRQVL AALYRLARVG FVTPLRDGMN LVAKEYVSAQ NPDDPGVLVL SRFAGAAREL TGALIVNPID IDGMADALSQ ALTMPLAERR ARYADMIAQL RENNVSVWRD NFLRDLQRV
NT Sequence
ATGAGTCGAC TCATCATCGT TTCAAACCGC GTCGCGCCGA TTTCGGAAGG CGAACCGGCG GCGGGTGGCC TCGCGATCGG CGTCTACGAC GCGCTGAAGG AGACGGGCGG CATGTGGTTC GGCTGGAGCG GCGAAGTGGT CGCATCCGGT GCGCCGCAGA TCCGCATCGA GGAGCGCGGC CCCGTGACGT TCGCGACCGT CGGGCTGTCG CGCCGCGATT ACGACCAGTA CTACCGCGGT TTCTCGAACG CGACGCTGTG GCCCGCGTTC CATTACCGCG CCGACCTGAT CCAGTACGAC CGCCACGAGT TTGACGGCTA CCGCCGCGTG AACGTGTGGC TCGCGCAGCA GCTCGTGCCG CTGCTGCAGG ACGACGACGT GATCTGGGTG CACGACTATC ACCTGATCCC GTTCGCGCGT GCGCTGCGTG AGGCCGGCGT GAAGAACCGC ATCGGCTTTT TCCTGCACAT TCCGTTTCCG GCCGCGCAGG TGCTCGTCAA CGTGCCGCCG CATCGCGAGC TCGTCGAGTC GCTGTGCGCG TTCGATTTGC TCGGCTTCCA GACCGAGCCC GACCTGCGCG CGTTCTGCGA CTACATCGAG TTCGAGGCGG GCGGCGAAGT CGCGCGGGGA GGGCACACGG TGCGCGTGAA CGCGTTCGGC AACACGCTGC GCGCGGCCGC CTATCCGATC GGCGTGTATC CGGACGAGAT CGCGTCGCTC GCGCAGGCGG CCGAGAACGG CAAGGCCGTG CGCACGCTGG CGACGTCGCT GCGCGGGCGC CAGTTGATCA TGAGCGTCGA CCGGCTCGAT TATTCGAAGG GGCTCGTCGA GCGGTTCCGT GCGTTCGAGA AGCTGCTGGA GCACCAGACG TCGATTCGCA ATCGCGTGTC GTTCCTGCAG ATCGCGCCGT CGACGCGCGC CGACCTGCGC GCGTACCAGG ACATCCGCCT GCAGCTCGAA GCGGAGTCGG GGCGCATCAA CGGCCGCTAC GCGGAGCTCG ACTGGGCGCC GATCCTCTAC ATTCACCGCC AGTACGACCG GCAGGTGCTG GCTGCGCTGT ACCGGCTGGC GCGCGTGGGT TTCGTCACGC CGTTGCGCGA CGGCATGAAC CTCGTCGCGA AGGAGTACGT GTCCGCGCAG AACCCGGACG ATCCGGGCGT GCTGGTGCTG TCGCGCTTCG CGGGGGCCGC GCGCGAGCTG ACGGGCGCGT TGATCGTCAA TCCGATCGAC ATCGACGGGA TGGCCGATGC GCTGTCGCAG GCGCTGACGA TGCCGCTCGC CGAGCGGCGC GCGCGCTATG CGGACATGAT CGCGCAGCTG CGCGAGAACA ACGTGTCGGT GTGGCGCGAC AACTTCCTGC GCGACCTGCA GCGCGTG
Details for BuamA.00046.b.B1.GE39247
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 53kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMS RLIIVSNRVA PISEGEPAAG GLAIGVYDAL KETGGMWFGW SGEVVASGAP QIRIEERGPV TFATVGLSRR DYDQYYRGFS NATLWPAFHY RADLIQYDRH EFDGYRRVNV WLAQQLVPLL QDDDVIWVHD YHLIPFARAL REAGVKNRIG FFLHIPFPAA QVLVNVPPHR ELVESLCAFD LLGFQTEPDL RAFCDYIEFE AGGEVARGGH TVRVNAFGNT LRAAAYPIGV YPDEIASLAQ AAENGKAVRT LATSLRGRQL IMSVDRLDYS KGLVERFRAF EKLLEHQTSI RNRVSFLQIA PSTRADLRAY QDIRLQLEAE SGRINGRYAE LDWAPILYIH RQYDRQVLAA LYRLARVGFV TPLRDGMNLV AKEYVSAQNP DDPGVLVLSR FAGAARELTG ALIVNPIDID GMADALSQAL TMPLAERRAR YADMIAQLRE NNVSVWRDNF LRDLQRV
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtc gactcatcat cgtttcaaac cgcgtcgcgc cgatttcgga aggcgaaccg gcggcgggtg gcctcgcgat cggcgtctac gacgcgctga aggagacggg cggcatgtgg ttcggctgga gcggcgaagt ggtcgcatcc ggtgcgccgc agatccgcat cgaggagcgc ggccccgtga cgttcgcgac cgtcgggctg tcgcgccgcg attacgacca gtactaccgc ggtttctcga acgcgacgct gtggcccgcg ttccattacc gcgccgacct gatccagtac gaccgccacg agtttgacgg ctaccgccgc gtgaacgtgt ggctcgcgca gcagctcgtg ccgctgctgc aggacgacga cgtgatctgg gtgcacgact atcacctgat cccgttcgcg cgtgcgctgc gtgaggccgg cgtgaagaac cgcatcggct ttttcctgca cattccgttt ccggccgcgc aggtgctcgt caacgtgccg ccgcatcgcg agctcgtcga gtcgctgtgc gcgttcgatt tgctcggctt ccagaccgag cccgacctgc gcgcgttctg cgactacatc gagttcgagg cgggcggcga agtcgcgcgg ggagggcaca cggtgcgcgt gaacgcgttc ggcaacacgc tgcgcgcggc cgcctatccg atcggcgtgt atccggacga gatcgcgtcg ctcgcgcagg cggccgagaa cggcaaggcc gtgcgcacgc tggcgacgtc gctgcgcggg cgccagttga tcatgagcgt cgaccggctc gattattcga aggggctcgt cgagcggttc cgtgcgttcg agaagctgct ggagcaccag acgtcgattc gcaatcgcgt gtcgttcctg cagatcgcgc cgtcgacgcg cgccgacctg cgcgcgtacc aggacatccg cctgcagctc gaagcggagt cggggcgcat caacggccgc tacgcggagc tcgactgggc gccgatcctc tacattcacc gccagtacga ccggcaggtg ctggctgcgc tgtaccggct ggcgcgcgtg ggtttcgtca cgccgttgcg cgacggcatg aacctcgtcg cgaaggagta cgtgtccgcg cagaacccgg acgatccggg cgtgctggtg ctgtcgcgct tcgcgggggc cgcgcgcgag ctgacgggcg cgttgatcgt caatccgatc gacatcgacg ggatggccga tgcgctgtcg caggcgctga cgatgccgct cgccgagcgg cgcgcgcgct atgcggacat gatcgcgcag ctgcgcgaga acaacgtgtc ggtgtggcgc gacaacttcc tgcgcgacct gcagcgcgtg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat