BuamA.00036.a
Phosphoribosylaminoimidazole carboxylase, ATPase subunit
| CENTER ID: | BuamA.00036.a |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00036.a.A1.GE33107 | Full length( BuamA.00036.a ) | 1 | 398 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00036.a.A1.PS01234 | Full length( BuamA.00036.a ) | 1 | 398 |
Structures
4E4T
DEPOSITED: 3/13/2012
DETERMINATION: XRay
CLONE: BuamA.00036.a.A1.GE33107
PROTEIN: BuamA.00036.a.A1.PS01234
Publications by SSGCID
Combining functional and structural genomics to sample the essential Burkholderia structome.
Abendroth J, Armour B, Barrett L, Baugh L, Begley DW, Buchko GW, Choi R, Clifton MC, Dieterich SH, Dranow DM, Edwards TE, Fairman JW, Fox D, Gallagher LA, Gardberg AS, Gillespie A, Manoil C, Myler PJ, Nakazawa-Hewitt S, Napuli A, Nguyen MT, Patrapuvich R, Phan I, Stacy R, Staker BL, Stewart LJ, Van Voorhis WC
PLoS ONE - 2012
volume 8, issue 1, pages e53851
PMID: 23382856; PMCID: PMC3561365
PDB:
3D63,
3DAH,
3EIZ,
3EJ2,
3EK2,
3EZO,
3F0F,
3FTP,
3GK0,
3GK3,
3GVF,
3GWA,
3GWE,
3IML,
3SZ8,
3T4C,
3TML,
3TMQ,
3TXY,
3U7J,
3UE9,
3UK1,
3UK2,
3UND,
3UPT,
3URR,
3UW1,
3UW2,
3UW3,
3V2I,
3V7N,
3V8H,
3V9O,
3V9P,
3VAV,
4DDO,
4DFE,
4DHE,
4DHK,
4DUT,
4DZ4,
4E4T,
4E51,
4EFI,
4EG0,
4EGJ,
4EK2,
4EQY,
4EWG,
4EX5,
4EXQ,
4F2G,
4F32,
4F3N,
4F3Y,
4F4H,
4F7D,
4FK8,
4FRY,
4G1K,
4G67,
4G6Z,
4GHK,
4H3Y,
4H3Z,
4H4G
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127668 |
| PATRIC ID: | fig|398577.6.peg.2618 |
| RefSeq: | YP_001809236.1 |
| UniProt: | B1YVV9 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTATPDSVSP ILPGAWLGMV GGGQLGRMFC FAAQSMGYRV AVLDPDPASP AGAVADRHLR AAYDDEAALA ELAGLCEAVS TEFENVPAAS LDFLARTTFV APAGRCVAVA QDRIAEKRFI EASGVPVAPH VVIESAAALA ALDDAALDAV LPGILKTARL GYDGKGQVRV STAREARDAH AALGGVPCVL EKRLPLKYEV SALIARGADG RSAAFPLAQN VHHNGILALT IVPAPAADTA RVEEAQQAAV RIADTLGYVG VLCVEFFVLE DGSFVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTRMP LGNPRQHSPA AMLNILGDVW FPNGAAAGAV TPPWDTVAAM PAAHLHLYGK EEARVGRKMG HVNFTAEMRD DAVAAATACA QLLRVPLD
NT Sequence
ATGACCGCAA CTCCTGATTC CGTTTCCCCG ATCCTGCCCG GCGCGTGGCT GGGCATGGTC GGCGGTGGCC AGCTCGGCCG CATGTTCTGC TTTGCCGCCC AGTCGATGGG CTATCGCGTC GCCGTGCTCG ATCCCGATCC GGCCAGCCCC GCCGGCGCGG TCGCCGACCG CCACCTGCGC GCGGCCTACG ACGACGAAGC CGCGCTCGCC GAGCTGGCCG GACTGTGCGA GGCCGTGTCG ACGGAATTCG AGAACGTGCC GGCCGCGAGC CTCGATTTCC TCGCGCGCAC GACGTTCGTC GCGCCGGCCG GCCGCTGCGT CGCGGTCGCG CAGGACCGGA TCGCGGAGAA GCGTTTCATC GAGGCCTCCG GCGTGCCGGT CGCGCCGCAC GTCGTGATCG AATCGGCGGC GGCACTGGCC GCGCTCGACG ATGCCGCGCT CGATGCGGTG CTGCCGGGCA TCCTGAAGAC GGCGCGTCTC GGCTACGACG GCAAGGGCCA GGTGCGCGTG AGCACCGCGC GGGAAGCGCG CGATGCGCAT GCGGCGCTCG GCGGCGTGCC GTGCGTGCTC GAAAAGCGCC TGCCGCTGAA GTACGAAGTG TCCGCGCTGA TCGCGCGCGG CGCGGACGGC CGCTCGGCCG CCTTCCCGCT CGCGCAGAAC GTGCATCACA ACGGCATCCT CGCGCTGACC ATCGTGCCGG CGCCGGCGGC TGACACCGCG CGTGTCGAAG AGGCGCAGCA GGCGGCCGTC CGGATCGCCG ATACGCTCGG CTACGTCGGC GTGCTGTGCG TCGAATTCTT CGTGCTGGAA GACGGCTCGT TCGTCGCGAA CGAAATGGCG CCGCGCCCGC ACAACTCCGG CCACTACACG GTCGATGCGT GCGCAACGAG CCAGTTCGAG CAGCAGGTGC GCGCGATGAC GCGCATGCCG CTCGGCAACC CGCGCCAGCA TTCGCCGGCC GCGATGCTGA ACATCCTCGG CGACGTGTGG TTCCCCAATG GTGCGGCGGC CGGGGCCGTC ACGCCGCCGT GGGACACGGT CGCCGCGATG CCGGCCGCGC ACCTGCACCT GTACGGCAAG GAAGAAGCGC GCGTCGGCCG CAAGATGGGC CACGTGAACT TCACGGCCGA GATGCGCGAC GACGCCGTCG CCGCCGCCAC CGCGTGTGCG CAGCTGCTGC GCGTGCCGCT CGAC
Details for BuamA.00036.a.A1.GE33107
| HARVESTED ON: | 8/19/2011 |
| SEQUENCED ON: | 8/31/2011 |
| EXPECTED MW: | 43kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 92 |
Validated AA Sequence
MTATPDSVSP ILPGAWLGMV GGGQLGRMFC FAAQSMGYRV AVLDPDPASP AGAVADRHLR AAYDDEAALA ELAGLCEAVS TEFENVPAAS LDFLARTTFV APAGRCVAVA QDRIAEKRFI EASGVPVAPH VVIESAAALA ALDDAALDAV LPGILKTARL GYDGXGQVRV STAREARDAH AALGGVPCVL EKRLPLKYEV SALIARGADG RSAAFPLAQN VHHNGILALT IVPAPAADTA RVEEAQQAAV RIADTLGYVG VLCVEFFVLE DGSFVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTRMP LGNPRQHSPA AMLNILGDVW FPNGAAAGAV TPPWDTVAAM PAAHLHLYGK EEARVGRKMG HVNFTAEMRD DAVAAA
Validated NT Sequence
ggtggcggcg gcgacggcgt cgtcgcgcat ctcggccgtg aagttcacgt ggcccatctt gcggccgacg cgcgcttctt ccttgccgta caggtgcagg tgcgcggccg gcatcgcggc gaccgtgtcc cacggcggcg tgacggcccc ggccgccgca ccattgggga accacacgtc gccgaggatg ttcagcatcg cggccggcga atgctggcgc gggttgccga gcggcatgcg cgtcatcgcg cgcacctgct gctcgaactg gctcgttgcg cacgcatcga ccgtgtagtg gccggagttg tgcgggcgcg gcgccatttc gttcgcgacg aacgagccgt cttccagcac gaagaattcg acgcacagca cgccgacgta gccgagcgta tcggcgatcc ggacggccgc ctgctgcgcc tcttcgacac gcgcggtgtc agccgccggc gccggcacga tggtcagcgc gaggatgccg ttgtgatgca cgttctgcgc gagcgggaag gcggccgagc ggccgtccgc gccgcgcgcg atcagcgcgg acacttcgta cttcagcggc aggcgctttt cgagcacgca cggcacgccg ccgagcgccg catgcgcatc gcgcgcttcc cgcgcggtgc tcacgcgcac ctggccctng ccgtcgtagc cgagacgcgc cgtcttcagg atgcccggca gcaccgcatc gagcgcggca tcgtcgagcg cggccagtgc cgccgccgat tcgatcacga cgtgcggcgc gaccggcacg ccggaggcct cgatgaaacg cttctccgcg atccggtcct gcgcgaccgc gacgcagcgg ccggccggcg cgacgaacgt cgtgcgcgcg aggaaatcga ggctcgcggc cggcacgttc tcgaattccg tcgacacggc ctcgcacagt ccggccagct cggcgagcgc ggcttcgtcg tcgtaggccg cgcgcaggtg gcggtcggcg accgcgccgg cggggctggc cggatcggga tcgagcacgg cgacgcgata gcccatcgac tgggcggcaa agcagaacat gcggccgagc tggccaccgc cgaccatgcc cagccacgcg ccgggcagga tcggggaaac ggaatcagga gttgcggtca tcgaaccagg accctgggtc tgagcttcca
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTATPDSVS PILPGAWLGM VGGGQLGRMF CFAAQSMGYR VAVLDPDPAS PAGAVADRHL RAAYDDEAAL AELAGLCEAV STEFENVPAA SLDFLARTTF VAPAGRCVAV AQDRIAEKRF IEASGVPVAP HVVIESAAAL AALDDAALDA VLPGILKTAR LGYDGKGQVR VSTAREARDA HAALGGVPCV LEKRLPLKYE VSALIARGAD GRSAAFPLAQ NVHHNGILAL TIVPAPAADT ARVEEAQQAA VRIADTLGYV GVLCVEFFVL EDGSFVANEM APRPHNSGHY TVDACATSQF EQQVRAMTRM PLGNPRQHSP AAMLNILGDV WFPNGAAAGA VTPPWDTVAA MPAAHLHLYG KEEARVGRKM GHVNFTAEMR DDAVAAATAC AQLLRVPLD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgcaact cctgattccg tttccccgat cctgcccggc gcgtggctgg gcatggtcgg cggtggccag ctcggccgca tgttctgctt tgccgcccag tcgatgggct atcgcgtcgc cgtgctcgat cccgatccgg ccagccccgc cggcgcggtc gccgaccgcc acctgcgcgc ggcctacgac gacgaagccg cgctcgccga gctggccgga ctgtgcgagg ccgtgtcgac ggaattcgag aacgtgccgg ccgcgagcct cgatttcctc gcgcgcacga cgttcgtcgc gccggccggc cgctgcgtcg cggtcgcgca ggaccggatc gcggagaagc gtttcatcga ggcctccggc gtgccggtcg cgccgcacgt cgtgatcgaa tcggcggcgg cactggccgc gctcgacgat gccgcgctcg atgcggtgct gccgggcatc ctgaagacgg cgcgtctcgg ctacgacggc aagggccagg tgcgcgtgag caccgcgcgg gaagcgcgcg atgcgcatgc ggcgctcggc ggcgtgccgt gcgtgctcga aaagcgcctg ccgctgaagt acgaagtgtc cgcgctgatc gcgcgcggcg cggacggccg ctcggccgcc ttcccgctcg cgcagaacgt gcatcacaac ggcatcctcg cgctgaccat cgtgccggcg ccggcggctg acaccgcgcg tgtcgaagag gcgcagcagg cggccgtccg gatcgccgat acgctcggct acgtcggcgt gctgtgcgtc gaattcttcg tgctggaaga cggctcgttc gtcgcgaacg aaatggcgcc gcgcccgcac aactccggcc actacacggt cgatgcgtgc gcaacgagcc agttcgagca gcaggtgcgc gcgatgacgc gcatgccgct cggcaacccg cgccagcatt cgccggccgc gatgctgaac atcctcggcg acgtgtggtt ccccaatggt gcggcggccg gggccgtcac gccgccgtgg gacacggtcg ccgcgatgcc ggccgcgcac ctgcacctgt acggcaagga agaagcgcgc gtcggccgca agatgggcca cgtgaacttc acggccgaga tgcgcgacga cgccgtcgcc gccgccaccg cgtgtgcgca gctgctgcgc gtgccgctcg actaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for BuamA.00036.a.A1.PS01234
| PURIFICATION DATe: | 10/12/2011 |
| CONCENTRATION: | 51.32mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 92 |
Protocol Notes
notes unavailable
Validated AA Sequence
MTATPDSVSP ILPGAWLGMV GGGQLGRMFC FAAQSMGYRV AVLDPDPASP AGAVADRHLR AAYDDEAALA ELAGLCEAVS TEFENVPAAS LDFLARTTFV APAGRCVAVA QDRIAEKRFI EASGVPVAPH VVIESAAALA ALDDAALDAV LPGILKTARL GYDGXGQVRV STAREARDAH AALGGVPCVL EKRLPLKYEV SALIARGADG RSAAFPLAQN VHHNGILALT IVPAPAADTA RVEEAQQAAV RIADTLGYVG VLCVEFFVLE DGSFVANEMA PRPHNSGHYT VDACATSQFE QQVRAMTRMP LGNPRQHSPA AMLNILGDVW FPNGAAAGAV TPPWDTVAAM PAAHLHLYGK EEARVGRKMG HVNFTAEMRD DAVAAA
Validated NT Sequence
ggtggcggcg gcgacggcgt cgtcgcgcat ctcggccgtg aagttcacgt ggcccatctt gcggccgacg cgcgcttctt ccttgccgta caggtgcagg tgcgcggccg gcatcgcggc gaccgtgtcc cacggcggcg tgacggcccc ggccgccgca ccattgggga accacacgtc gccgaggatg ttcagcatcg cggccggcga atgctggcgc gggttgccga gcggcatgcg cgtcatcgcg cgcacctgct gctcgaactg gctcgttgcg cacgcatcga ccgtgtagtg gccggagttg tgcgggcgcg gcgccatttc gttcgcgacg aacgagccgt cttccagcac gaagaattcg acgcacagca cgccgacgta gccgagcgta tcggcgatcc ggacggccgc ctgctgcgcc tcttcgacac gcgcggtgtc agccgccggc gccggcacga tggtcagcgc gaggatgccg ttgtgatgca cgttctgcgc gagcgggaag gcggccgagc ggccgtccgc gccgcgcgcg atcagcgcgg acacttcgta cttcagcggc aggcgctttt cgagcacgca cggcacgccg ccgagcgccg catgcgcatc gcgcgcttcc cgcgcggtgc tcacgcgcac ctggccctng ccgtcgtagc cgagacgcgc cgtcttcagg atgcccggca gcaccgcatc gagcgcggca tcgtcgagcg cggccagtgc cgccgccgat tcgatcacga cgtgcggcgc gaccggcacg ccggaggcct cgatgaaacg cttctccgcg atccggtcct gcgcgaccgc gacgcagcgg ccggccggcg cgacgaacgt cgtgcgcgcg aggaaatcga ggctcgcggc cggcacgttc tcgaattccg tcgacacggc ctcgcacagt ccggccagct cggcgagcgc ggcttcgtcg tcgtaggccg cgcgcaggtg gcggtcggcg accgcgccgg cggggctggc cggatcggga tcgagcacgg cgacgcgata gcccatcgac tgggcggcaa agcagaacat gcggccgagc tggccaccgc cgaccatgcc cagccacgcg ccgggcagga tcggggaaac ggaatcagga gttgcggtca tcgaaccagg accctgggtc tgagcttcca
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTATPDSVS PILPGAWLGM VGGGQLGRMF CFAAQSMGYR VAVLDPDPAS PAGAVADRHL RAAYDDEAAL AELAGLCEAV STEFENVPAA SLDFLARTTF VAPAGRCVAV AQDRIAEKRF IEASGVPVAP HVVIESAAAL AALDDAALDA VLPGILKTAR LGYDGKGQVR VSTAREARDA HAALGGVPCV LEKRLPLKYE VSALIARGAD GRSAAFPLAQ NVHHNGILAL TIVPAPAADT ARVEEAQQAA VRIADTLGYV GVLCVEFFVL EDGSFVANEM APRPHNSGHY TVDACATSQF EQQVRAMTRM PLGNPRQHSP AAMLNILGDV WFPNGAAAGA VTPPWDTVAA MPAAHLHLYG KEEARVGRKM GHVNFTAEMR DDAVAAATAC AQLLRVPLD
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACCGCAACT CCTGATTCCG TTTCCCCGAT CCTGCCCGGC GCGTGGCTGG GCATGGTCGG CGGTGGCCAG CTCGGCCGCA TGTTCTGCTT TGCCGCCCAG TCGATGGGCT ATCGCGTCGC CGTGCTCGAT CCCGATCCGG CCAGCCCCGC CGGCGCGGTC GCCGACCGCC ACCTGCGCGC GGCCTACGAC GACGAAGCCG CGCTCGCCGA GCTGGCCGGA CTGTGCGAGG CCGTGTCGAC GGAATTCGAG AACGTGCCGG CCGCGAGCCT CGATTTCCTC GCGCGCACGA CGTTCGTCGC GCCGGCCGGC CGCTGCGTCG CGGTCGCGCA GGACCGGATC GCGGAGAAGC GTTTCATCGA GGCCTCCGGC GTGCCGGTCG CGCCGCACGT CGTGATCGAA TCGGCGGCGG CACTGGCCGC GCTCGACGAT GCCGCGCTCG ATGCGGTGCT GCCGGGCATC CTGAAGACGG CGCGTCTCGG CTACGACGGC AAGGGCCAGG TGCGCGTGAG CACCGCGCGG GAAGCGCGCG ATGCGCATGC GGCGCTCGGC GGCGTGCCGT GCGTGCTCGA AAAGCGCCTG CCGCTGAAGT ACGAAGTGTC CGCGCTGATC GCGCGCGGCG CGGACGGCCG CTCGGCCGCC TTCCCGCTCG CGCAGAACGT GCATCACAAC GGCATCCTCG CGCTGACCAT CGTGCCGGCG CCGGCGGCTG ACACCGCGCG TGTCGAAGAG GCGCAGCAGG CGGCCGTCCG GATCGCCGAT ACGCTCGGCT ACGTCGGCGT GCTGTGCGTC GAATTCTTCG TGCTGGAAGA CGGCTCGTTC GTCGCGAACG AAATGGCGCC GCGCCCGCAC AACTCCGGCC ACTACACGGT CGATGCGTGC GCAACGAGCC AGTTCGAGCA GCAGGTGCGC GCGATGACGC GCATGCCGCT CGGCAACCCG CGCCAGCATT CGCCGGCCGC GATGCTGAAC ATCCTCGGCG ACGTGTGGTT CCCCAATGGT GCGGCGGCCG GGGCCGTCAC GCCGCCGTGG GACACGGTCG CCGCGATGCC GGCCGCGCAC CTGCACCTGT ACGGCAAGGA AGAAGCGCGC GTCGGCCGCA AGATGGGCCA CGTGAACTTC ACGGCCGAGA TGCGCGACGA CGCCGTCGCC GCCGCCACCG CGTGTGCGCA GCTGCTGCGC GTGCCGCTCG ACTAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA