BuamA.00027.c

Acyl-CoA dehydrogenase domain protein

CENTER ID: BuamA.00027.c
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00027.c.B1.GE39925 Full length( BuamA.00027.c ) 1 398
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2206
RefSeq: YP_001808844.1
UniProt: B1YTP0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLDYSPADD AFRVDVRAWL EANLPHALRA KVLDHKRLDR EDFASWHRIL GQRGWSAPAW PAEYGGPGWN ATQRHIWDEE CARIGAPTVL PFGVSMVAPV LMKYGSEAQK RHYLPRILDG TDWWCQGYSE PGSGSDLASL RTRAERHGDH YVVNGQKTWT TLGQYADMMF CLVRTDPAAK KQEGISFLLI DMATPGITVR PIITLDEDHE VNEVFFEDVK VPVENLVGDE NRGWTYAKYL LGHERTGIAR VGASKRELVF LKRVASHQQK NGKPLLADPV FAAKVAALEV ELMALEVTVL RVVSRETSGK GPGPEASMLK IKGTEVQQAL TELMFEAIGP LAAPFDVPFL EGKREHSIAG DDDAAPLAAY YFNYRKTSIY GGSNEIQKNI IAQMILGL
NT Sequence
ATGGATCTGG ATTATTCCCC CGCCGACGAC GCGTTCCGCG TTGACGTTCG CGCCTGGCTC GAGGCCAATC TGCCTCACGC CCTGCGCGCC AAGGTACTCG ATCACAAACG ACTCGACCGC GAGGACTTCG CGAGCTGGCA CCGGATTCTC GGCCAGCGCG GCTGGTCCGC GCCCGCGTGG CCTGCTGAAT ACGGCGGCCC GGGCTGGAAC GCGACGCAGC GGCACATCTG GGACGAGGAG TGCGCCCGCA TCGGCGCGCC GACCGTGCTT CCGTTCGGCG TGTCGATGGT CGCGCCGGTG CTGATGAAAT ACGGCAGCGA AGCGCAGAAG CGCCACTATC TGCCGCGCAT CCTCGACGGC ACCGACTGGT GGTGTCAGGG CTATTCGGAG CCCGGCTCCG GGTCGGACCT CGCGTCGCTG CGCACCCGCG CCGAACGCCA TGGTGATCAC TACGTCGTCA ACGGCCAGAA GACCTGGACT ACGCTCGGCC AGTACGCGGA CATGATGTTC TGCCTCGTGC GCACGGATCC GGCCGCGAAG AAGCAGGAGG GCATCTCGTT CCTGCTGATC GACATGGCGA CGCCCGGCAT CACGGTGCGT CCGATCATCA CGCTCGACGA GGATCACGAG GTCAATGAGG TGTTCTTCGA GGACGTGAAG GTGCCCGTCG AGAATCTCGT CGGCGACGAA AACCGCGGCT GGACCTACGC GAAGTACCTG CTGGGCCACG AGCGCACCGG TATCGCGCGC GTCGGCGCGT CGAAGCGCGA GCTCGTGTTC CTGAAGCGCG TGGCATCGCA CCAGCAGAAG AACGGCAAGC CGTTGCTCGC GGATCCCGTG TTTGCCGCGA AGGTCGCCGC GCTCGAGGTC GAGCTGATGG CGCTCGAGGT GACGGTGCTG CGCGTCGTGA GCCGCGAGAC GAGCGGCAAG GGGCCGGGCC CCGAGGCGTC GATGCTGAAG ATCAAGGGTA CCGAGGTGCA GCAGGCGCTC ACCGAGCTGA TGTTCGAGGC GATCGGGCCG CTCGCGGCGC CGTTCGACGT GCCGTTCCTC GAGGGCAAGC GCGAACACAG CATCGCGGGC GACGACGATG CCGCGCCGCT CGCCGCGTAC TACTTCAATT ATCGGAAGAC GTCGATCTAC GGCGGCTCGA ACGAAATTCA GAAGAACATC ATCGCGCAGA TGATTCTCGG GCTG
Details for BuamA.00027.c.B1.GE39925
HARVESTED ON: 2/26/2016
SEQUENCED ON: 3/1/2016
EXPECTED MW: 45kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 96
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMD LDYSPADDAF RVDVRAWLEA NLPHALRAKV LDHKRLDRED FASWHRILGQ RGWSAPAWPA EYGGPGWNAT QRHIWDEECA RIGAPTVLPF GVSMVAPVLM KYGSEAQKRH YLPRILDGTD WWCQGYSEPG SGSDLASLRT RAERHGDHYV VNGQKTWTTL GQYADMMFCL VRTDPAAKKQ EGISFLLIDM ATPGITVRPI ITLDEDHEVN EVFFEDVKVP VENLVGDENR GWTYAKYLLG HERTGIARVG AXKRELVXXK RVASHQQKNG KPLLADPVFA AKVAALEVEL MALEVTVXRV VSRETSGKGP GPEASMXKIK GTEVQQALTE XMFEAIGPLA XXXDVPFXEG KREHSIAGDD DAAPLAAXXF NYRKTSIYGG SNEIQKNI
Validated NT Sequence
gatgatgttc ttctgaattt cgttcgagcc gccgtagatc gacgtcttcc gataattgaa gnnnnacgcg gcgagcggcg cggcatcgtc gtcgcccgcg atgctgtgtt cgcgcttgcc ctcnnggaac ggcacgtcgn acgnnnccgc gagcggcccg atcgcctcga acatcngctc ggtgagcgcc tgctgcacct cggtaccctt gatcttcngc atcgacgcct cggggcccgg ccccttgccg ctcgtctcgc ggctcacgac gcgcnncacc gtcacctcga gcgccatcag ctcgacctcg agcgcggcga ccttcgcggc aaacacggga tccgcgagca acggcttgcc gttcttctgc tggtgcgatg ccacgcgctt cnnnaacacg agctcgcgct tcgnngcgcc gacgcgcgcg ataccggtgc gctcgtggcc cagcaggtac ttcgcgtagg tccagccgcg gttttcgtcg ccgacgagat tctcgacggg caccttcacg tcctcgaaga acacctcatt gacctcgtga tcctcgtcga gcgtgatgat cggacgcacc gtgatgccgg gcgtcgccat gtcgatcagc aggaacgaga tgccctcctg cttcttcgcg gccggatccg tgcgcacgag gcagaacatc atgtccgcgt actggccgag cgtagtccag gtcttctggc cgttgacgac gtagtgatca ccatggcgtt cggcgcgggt gcgcagcgac gcgaggtccg acccggagcc gggctccgaa tagccctgac accaccagtc ggtgccgtcg aggatgcgcg gcagatagtg gcgcttctgc gcttcgctgc cgtatttcat cagcaccggc gcgaccatcg acacgccgaa cggaagcacg gtcggcgcgc cgatgcgggc gcactcctcg tcccagatgt gccgctgcgt cgcgttccag cccgggccgc cgtattcagc aggccacgcg ggcgcggacc agccgcgctg gccgagaatc cggtgccagc tcgcgaagtc ctcgcggtcg agtcgtttgt gatcgagtac cttggcgcgc agggcgtgag gcagattggc ctcgagccag gcgcgaacgt caacgcggaa cgcgtcgtcg gcgggggaat aatccagatc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMD LDYSPADDAF RVDVRAWLEA NLPHALRAKV LDHKRLDRED FASWHRILGQ RGWSAPAWPA EYGGPGWNAT QRHIWDEECA RIGAPTVLPF GVSMVAPVLM KYGSEAQKRH YLPRILDGTD WWCQGYSEPG SGSDLASLRT RAERHGDHYV VNGQKTWTTL GQYADMMFCL VRTDPAAKKQ EGISFLLIDM ATPGITVRPI ITLDEDHEVN EVFFEDVKVP VENLVGDENR GWTYAKYLLG HERTGIARVG ASKRELVFLK RVASHQQKNG KPLLADPVFA AKVAALEVEL MALEVTVLRV VSRETSGKGP GPEASMLKIK GTEVQQALTE LMFEAIGPLA APFDVPFLEG KREHSIAGDD DAAPLAAYYF NYRKTSIYGG SNEIQKNIIA QMILGL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc tggattattc ccccgccgac gacgcgttcc gcgttgacgt tcgcgcctgg ctcgaggcca atctgcctca cgccctgcgc gccaaggtac tcgatcacaa acgactcgac cgcgaggact tcgcgagctg gcaccggatt ctcggccagc gcggctggtc cgcgcccgcg tggcctgctg aatacggcgg cccgggctgg aacgcgacgc agcggcacat ctgggacgag gagtgcgccc gcatcggcgc gccgaccgtg cttccgttcg gcgtgtcgat ggtcgcgccg gtgctgatga aatacggcag cgaagcgcag aagcgccact atctgccgcg catcctcgac ggcaccgact ggtggtgtca gggctattcg gagcccggct ccgggtcgga cctcgcgtcg ctgcgcaccc gcgccgaacg ccatggtgat cactacgtcg tcaacggcca gaagacctgg actacgctcg gccagtacgc ggacatgatg ttctgcctcg tgcgcacgga tccggccgcg aagaagcagg agggcatctc gttcctgctg atcgacatgg cgacgcccgg catcacggtg cgtccgatca tcacgctcga cgaggatcac gaggtcaatg aggtgttctt cgaggacgtg aaggtgcccg tcgagaatct cgtcggcgac gaaaaccgcg gctggaccta cgcgaagtac ctgctgggcc acgagcgcac cggtatcgcg cgcgtcggcg cgtcgaagcg cgagctcgtg ttcctgaagc gcgtggcatc gcaccagcag aagaacggca agccgttgct cgcggatccc gtgtttgccg cgaaggtcgc cgcgctcgag gtcgagctga tggcgctcga ggtgacggtg ctgcgcgtcg tgagccgcga gacgagcggc aaggggccgg gccccgaggc gtcgatgctg aagatcaagg gtaccgaggt gcagcaggcg ctcaccgagc tgatgttcga ggcgatcggg ccgctcgcgg cgccgttcga cgtgccgttc ctcgagggca agcgcgaaca cagcatcgcg ggcgacgacg atgccgcgcc gctcgccgcg tactacttca attatcggaa gacgtcgatc tacggcggct cgaacgaaat tcagaagaac atcatcgcgc agatgattct cgggctgtga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at