SSGCID: Target Page

BuamA.00027.b

Acyl-CoA dehydrogenase domain protein

CENTER ID:	BuamA.00027.b
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00027.b.B1.GE39924	Full length( BuamA.00027.b )		1	388

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00027.b.B1.PS37945	Full length( BuamA.00027.b )		1	388

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.1627
RefSeq:	YP_001808289.1
UniProt:	B1YQD5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQFDLTDDQR AIESAIEKIC ERFGDDYWLE RDRAGGFPSD FHAALAEAGW LGIAMDPAHG GAGLGMTEAA LMMRTISASG AGLSGASAVH MNIFGLNPVQ VFGNDAQKAR FLPPLIAGRD KACFAVTEPD AGLDTTHLST QARRDGDHYV LSGRKIWIST AQVANKMLII ARTTPLDAVA KPTEGLSLFY TDLDRSRVEV RAIEKMGRKA VDSNMLFIDN LRVPRDDLIG NEGDGFRYLL HGLNPERILI AAEAIGLGQA ALRRATQYAC ERVVFGRPIG QNQSIQHPLA QAWMQLEAAW LMVMKAATRY DAGQSCGAEA NAAKYLGAEA AFQACQTAIA THGGMGYAKE YHVERYLREC MIPRLAPVSP QLILCYIAEK VLGLPKSY

NT Sequence

ATGCAGTTCG ACCTGACCGA CGACCAGCGC GCGATCGAAA GCGCGATCGA GAAAATCTGC GAACGCTTCG GCGACGACTA CTGGCTCGAA CGCGATCGCG CGGGCGGGTT CCCGTCCGAC TTTCACGCGG CGCTCGCCGA GGCCGGCTGG CTCGGCATCG CGATGGACCC GGCCCACGGC GGCGCCGGCC TCGGGATGAC CGAGGCCGCG CTGATGATGC GCACGATCAG CGCGTCGGGC GCCGGCCTGT CGGGCGCCTC GGCCGTCCAC ATGAACATCT TCGGGCTGAA CCCGGTGCAG GTGTTCGGCA ACGACGCGCA GAAGGCGCGC TTCCTGCCGC CGCTGATCGC CGGGCGCGAC AAGGCCTGCT TCGCGGTGAC CGAGCCCGAC GCGGGCCTCG ACACGACGCA CCTGAGCACG CAGGCGCGCC GCGACGGCGA CCACTACGTG CTGAGCGGGC GCAAGATCTG GATCTCGACC GCGCAGGTCG CGAACAAGAT GCTGATCATC GCGCGCACGA CCCCGCTCGA TGCGGTCGCG AAGCCGACCG AGGGGCTCAG CCTGTTCTAC ACCGACCTCG ACCGGTCGCG CGTCGAGGTG CGCGCGATCG AGAAGATGGG CCGCAAGGCC GTCGATTCGA ACATGCTGTT CATCGACAAC CTGCGCGTGC CGCGCGACGA CCTGATCGGC AACGAGGGCG ACGGCTTCCG TTATCTGCTG CACGGGCTGA ACCCCGAGCG GATCCTGATC GCCGCCGAGG CGATCGGCCT CGGGCAGGCC GCGCTGCGGC GCGCGACACA GTATGCGTGC GAGCGTGTCG TGTTCGGCCG CCCGATCGGG CAGAACCAGT CGATCCAGCA TCCGCTCGCG CAGGCATGGA TGCAGCTCGA GGCCGCGTGG CTGATGGTGA TGAAGGCCGC GACGCGCTAC GACGCGGGGC AGTCGTGCGG GGCGGAGGCG AACGCCGCGA AGTACCTCGG CGCGGAAGCC GCGTTCCAGG CGTGCCAGAC GGCGATCGCG ACGCACGGCG GGATGGGCTA TGCGAAGGAA TACCATGTCG AGCGCTACCT GCGCGAGTGT ATGATTCCGA GGCTCGCGCC CGTGAGCCCG CAACTGATCC TCTGCTACAT CGCGGAGAAG GTGCTCGGGC TGCCGAAGTC GTAC

Details for BuamA.00027.b.B1.GE39924

HARVESTED ON:	2/26/2016
SEQUENCED ON:	3/1/2016
EXPECTED MW:	43kDa
OBSERVED MW:	43kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	78
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMQ FDLTDDQRAI ESAIEKICER FGDDYWLERD RAGGFPSDFH AALAEAGWLG IAMDPAHGGA GLGMTEAALM MRTISASGAG LSGASAVHMN IFGLNPVQVF GNDAQKARFL PPLIAGRDKA CFAVTEPDAG LDTTHLSTQA RRDGDHYVLS GRKIWISTAQ VANKMLIIAR TTPLDAVAKP TEGLSLFYTD LDRSRVEVRA IEKMGRKAVD SNMLFIDNLR VPRDDLIGNE GDGFRYLLHG LNPERILIAA EAIGLGQAAL RRAXTVCVRA CRVRPPDRAE PVDPASARAG MDAARGRVAD GDEGRDALRR GAVVRGGGER REVPRRGSRV PGVPDGDRDA RRDGLCEGIP CRALPARVYD SEARAREPAT DPLLHRGEG

Validated NT Sequence

gcaccttctc cgcgatgtag cagaggatca gttgcgggct cacgggcgcg agcctcggaa tcatacactc gcgcaggtag cgctcgacat ggtattcctt cgcatagccc atcccgccgt gcgtcgcgat cgccgtctgg cacgcctgga acgcggcttc cgcgccgagg tacttcgcgg cgttcgcctc cgccccgcac gactgccccg cgtcgtagcg cgtcgcggcc ttcatcacca tcagccacgc ggcctcgagc tgcatccatg cctgcgcgag cggatgctgg atcgactggt tctgcccgat cgggcggccg aacacgacac gctcgcacgc atactgtgtn cgcgcgccgc agcgcggcct gcccgaggcc gatcgcctcg gcggcgatca ggatccgctc ggggttcagc ccgtgcagca gataacggaa gccgtcgccc tcgttgccga tcaggtcgtc gcgcggcacg cgcaggttgt cgatgaacag catgttcgaa tcgacggcct tgcggcccat cttctcgatc gcgcgcacct cgacgcgcga ccggtcgagg tcggtgtaga acaggctgag cccctcggtc ggcttcgcga ccgcatcgag cggggtcgtg cgcgcgatga tcagcatctt gttcgcgacc tgcgcggtcg agatccagat cttgcgcccg ctcagcacgt agtggtcgcc gtcgcggcgc gcctgcgtgc tcaggtgcgt cgtgtcgagg cccgcgtcgg gctcggtcac cgcgaagcag gccttgtcgc gcccggcgat cagcggcggc aggaagcgcg ccttctgcgc gtcgttgccg aacacctgca ccgggttcag cccgaagatg ttcatgtgga cggccgaggc gcccgacagg ccggcgcccg acgcgctgat cgtgcgcatc atcagcgcgg cctcggtcat cccgaggccg gcgccgccgt gggccgggtc catcgcgatg ccgagccagc cggcctcggc gagcgccgcg tgaaagtcgg acgggaaccc gcccgcgcga tcgcgttcga gccagtagtc gtcgccgaag cgttcgcaga ttttctcgat cgcgctttcg atcgcgcgct ggtcgtcggt caggtcgaac tgcatatggt ggtggtggtg gtgagccat

Expected Protein Sequence

MAHHHHHHMQ FDLTDDQRAI ESAIEKICER FGDDYWLERD RAGGFPSDFH AALAEAGWLG IAMDPAHGGA GLGMTEAALM MRTISASGAG LSGASAVHMN IFGLNPVQVF GNDAQKARFL PPLIAGRDKA CFAVTEPDAG LDTTHLSTQA RRDGDHYVLS GRKIWISTAQ VANKMLIIAR TTPLDAVAKP TEGLSLFYTD LDRSRVEVRA IEKMGRKAVD SNMLFIDNLR VPRDDLIGNE GDGFRYLLHG LNPERILIAA EAIGLGQAAL RRATQYACER VVFGRPIGQN QSIQHPLAQA WMQLEAAWLM VMKAATRYDA GQSCGAEANA AKYLGAEAAF QACQTAIATH GGMGYAKEYH VERYLRECMI PRLAPVSPQL ILCYIAEKVL GLPKSY

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagt tcgacctgac cgacgaccag cgcgcgatcg aaagcgcgat cgagaaaatc tgcgaacgct tcggcgacga ctactggctc gaacgcgatc gcgcgggcgg gttcccgtcc gactttcacg cggcgctcgc cgaggccggc tggctcggca tcgcgatgga cccggcccac ggcggcgccg gcctcgggat gaccgaggcc gcgctgatga tgcgcacgat cagcgcgtcg ggcgccggcc tgtcgggcgc ctcggccgtc cacatgaaca tcttcgggct gaacccggtg caggtgttcg gcaacgacgc gcagaaggcg cgcttcctgc cgccgctgat cgccgggcgc gacaaggcct gcttcgcggt gaccgagccc gacgcgggcc tcgacacgac gcacctgagc acgcaggcgc gccgcgacgg cgaccactac gtgctgagcg ggcgcaagat ctggatctcg accgcgcagg tcgcgaacaa gatgctgatc atcgcgcgca cgaccccgct cgatgcggtc gcgaagccga ccgaggggct cagcctgttc tacaccgacc tcgaccggtc gcgcgtcgag gtgcgcgcga tcgagaagat gggccgcaag gccgtcgatt cgaacatgct gttcatcgac aacctgcgcg tgccgcgcga cgacctgatc ggcaacgagg gcgacggctt ccgttatctg ctgcacgggc tgaaccccga gcggatcctg atcgccgccg aggcgatcgg cctcgggcag gccgcgctgc ggcgcgcgac acagtatgcg tgcgagcgtg tcgtgttcgg ccgcccgatc gggcagaacc agtcgatcca gcatccgctc gcgcaggcat ggatgcagct cgaggccgcg tggctgatgg tgatgaaggc cgcgacgcgc tacgacgcgg ggcagtcgtg cggggcggag gcgaacgccg cgaagtacct cggcgcggaa gccgcgttcc aggcgtgcca gacggcgatc gcgacgcacg gcgggatggg ctatgcgaag gaataccatg tcgagcgcta cctgcgcgag tgtatgattc cgaggctcgc gcccgtgagc ccgcaactga tcctctgcta catcgcggag aaggtgctcg ggctgccgaa gtcgtactga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at

Details for BuamA.00027.b.B1.PS37945

PURIFICATION DATe:	6/16/2016
CONCENTRATION:	21.34mg/ml
OBSERVED MW:	41kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	200µl
PERCENT IDENTITY:	78
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMQ FDLTDDQRAI ESAIEKICER FGDDYWLERD RAGGFPSDFH AALAEAGWLG IAMDPAHGGA GLGMTEAALM MRTISASGAG LSGASAVHMN IFGLNPVQVF GNDAQKARFL PPLIAGRDKA CFAVTEPDAG LDTTHLSTQA RRDGDHYVLS GRKIWISTAQ VANKMLIIAR TTPLDAVAKP TEGLSLFYTD LDRSRVEVRA IEKMGRKAVD SNMLFIDNLR VPRDDLIGNE GDGFRYLLHG LNPERILIAA EAIGLGQAAL RRATQYACER VVFGRPIGQN QSIQHPLAQA WMQLEAAWLM VMKAATRYDA GQSCGAEANA AKYLGAEAAF QACQTAIATH GGMGYAKEYH VERYLRECMI PRLAPVSPQL ILCYIAEKVL GLPKSY

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAGT TCGACCTGAC CGACGACCAG CGCGCGATCG AAAGCGCGAT CGAGAAAATC TGCGAACGCT TCGGCGACGA CTACTGGCTC GAACGCGATC GCGCGGGCGG GTTCCCGTCC GACTTTCACG CGGCGCTCGC CGAGGCCGGC TGGCTCGGCA TCGCGATGGA CCCGGCCCAC GGCGGCGCCG GCCTCGGGAT GACCGAGGCC GCGCTGATGA TGCGCACGAT CAGCGCGTCG GGCGCCGGCC TGTCGGGCGC CTCGGCCGTC CACATGAACA TCTTCGGGCT GAACCCGGTG CAGGTGTTCG GCAACGACGC GCAGAAGGCG CGCTTCCTGC CGCCGCTGAT CGCCGGGCGC GACAAGGCCT GCTTCGCGGT GACCGAGCCC GACGCGGGCC TCGACACGAC GCACCTGAGC ACGCAGGCGC GCCGCGACGG CGACCACTAC GTGCTGAGCG GGCGCAAGAT CTGGATCTCG ACCGCGCAGG TCGCGAACAA GATGCTGATC ATCGCGCGCA CGACCCCGCT CGATGCGGTC GCGAAGCCGA CCGAGGGGCT CAGCCTGTTC TACACCGACC TCGACCGGTC GCGCGTCGAG GTGCGCGCGA TCGAGAAGAT GGGCCGCAAG GCCGTCGATT CGAACATGCT GTTCATCGAC AACCTGCGCG TGCCGCGCGA CGACCTGATC GGCAACGAGG GCGACGGCTT CCGTTATCTG CTGCACGGGC TGAACCCCGA GCGGATCCTG ATCGCCGCCG AGGCGATCGG CCTCGGGCAG GCCGCGCTGC GGCGCGCGAC ACAGTATGCG TGCGAGCGTG TCGTGTTCGG CCGCCCGATC GGGCAGAACC AGTCGATCCA GCATCCGCTC GCGCAGGCAT GGATGCAGCT CGAGGCCGCG TGGCTGATGG TGATGAAGGC CGCGACGCGC TACGACGCGG GGCAGTCGTG CGGGGCGGAG GCGAACGCCG CGAAGTACCT CGGCGCGGAA GCCGCGTTCC AGGCGTGCCA GACGGCGATC GCGACGCACG GCGGGATGGG CTATGCGAAG GAATACCATG TCGAGCGCTA CCTGCGCGAG TGTATGATTC CGAGGCTCGC GCCCGTGAGC CCGCAACTGA TCCTCTGCTA CATCGCGGAG AAGGTGCTCG GGCTGCCGAA GTCGTACTGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT