BuamA.00027.a

Acyl-CoA dehydrogenase domain protein

CENTER ID: BuamA.00027.a
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00027.a.B1.GE39923 Full length( BuamA.00027.a ) 1 381
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.2878
RefSeq: YP_001809487.1
UniProt: B1YNM1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFQHTEDRR MLADTLNRFI AEQYAFPVRD RIAQSADGFD RTMWQRFAEL GAVGALFAEA DGGFGGTGFD IAVVFECLGR GLVVEPFLGA LLAGRALSLA GGAAHRERLA ALIDGSASAA FAHDEPGSHY ELTTVRTRAE RAGDGWVLTG AKGVVDQAAQ AAFFVVSARV SGHDDDAAGI GLFVVPADAP GVSLRDYRKI DGGRAAEVRF GRVALPADAA LGGPSAHDGE AGAALLERVL GYGLLALSAE ALGAMDIAKE YTLDYLRTRK QFGMAIGSFQ ALQHRMADLL LEVEQARSAV INAAAQLDAP RAARERALAA AKYSIGRIGT LVAEESIQLH GGIGMTWELP LSHYAKRLVM IDHQLGDEDH HLARYIALSK Q
NT Sequence
ATGGATTTCC AGCACACAGA AGACCGCCGG ATGCTGGCGG ACACGTTGAA CCGCTTCATC GCCGAACAGT ACGCGTTCCC GGTGCGCGAT CGCATCGCGC AGTCGGCCGA CGGGTTCGAT CGCACGATGT GGCAGCGCTT CGCCGAACTC GGCGCGGTCG GCGCGCTGTT TGCCGAGGCC GACGGCGGCT TCGGCGGCAC GGGCTTCGAC ATCGCCGTGG TGTTCGAATG CCTCGGGCGC GGGCTCGTCG TCGAGCCGTT CCTCGGCGCG CTGCTCGCGG GCCGCGCGCT GTCGCTCGCC GGCGGCGCCG CGCATCGCGA GCGGCTTGCC GCGCTGATCG ACGGCAGCGC GAGCGCCGCG TTCGCGCACG ACGAGCCGGG CTCGCACTAC GAACTGACGA CGGTGCGCAC GCGCGCCGAG CGTGCCGGCG ACGGCTGGGT GCTGACGGGC GCGAAGGGCG TCGTCGACCA GGCCGCGCAG GCGGCGTTCT TCGTCGTCAG CGCGCGCGTG TCCGGTCACG ACGACGACGC GGCCGGCATC GGCCTGTTCG TCGTGCCGGC CGACGCGCCG GGCGTGTCGC TGCGCGACTA CCGGAAGATC GATGGTGGCC GTGCGGCCGA AGTGCGTTTC GGGCGCGTCG CGCTGCCGGC CGACGCCGCG CTCGGCGGGC CGAGTGCGCA CGACGGCGAA GCGGGCGCCG CGCTGCTCGA ACGCGTGCTC GGCTACGGGC TGCTCGCGCT GTCGGCCGAA GCGCTCGGCG CGATGGACAT CGCGAAGGAA TACACGCTCG ACTACCTGCG CACGCGCAAG CAGTTCGGGA TGGCGATCGG CAGCTTCCAG GCGCTGCAGC ACCGGATGGC CGACCTGCTG CTCGAAGTCG AGCAGGCGCG CTCGGCGGTG ATCAACGCGG CCGCGCAGCT CGATGCGCCG CGTGCGGCGC GCGAGCGCGC GCTCGCGGCG GCCAAGTACA GCATCGGCCG GATCGGCACG CTGGTCGCCG AGGAAAGCAT CCAGCTGCAC GGCGGGATCG GGATGACGTG GGAGCTGCCG CTGTCGCACT ACGCGAAGCG GCTGGTGATG ATCGATCACC AGCTCGGCGA CGAGGATCAT CATCTCGCGC GCTATATCGC GCTGTCGAAA CAG
Details for BuamA.00027.a.B1.GE39923
HARVESTED ON: 2/26/2016
SEQUENCED ON: 3/1/2016
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMD FQHTEDRRML ADTLNRFIAE QYAFPVRDRI AQSADGFDRT MWQRFAELGA VGALFAEADG GFGGTGFDIA VVFECLGRGL VVEPFLGALL AGRALSLAGG AAHRERLAAL IDGSASAAFA HDEPGSHYEL TTVRTRAERA GDGWVLTGAK GVVDQAAQAA FFVVSARVSG HDDDAAGIGL FVVPADAPGV SLRDYRKIDG GRAAEVRFGR VALPADAALG GPSAHDGEAG AALLERVLGY GLLALSAEAL GAMDIVKEYT LDYLRTRKQF GMAIGSFQAL QHRMADLLLE VEQARSAVIN AAAQLDAPRA ARERALAAAK YSIGRIGTLV AEESIQLHGG IGMTWELPLS HYAKRLVMID HQLGDEDHHL EGYIALSKQ
Validated NT Sequence
atggctcacc accaccacca ccatatggat ttccagcaca cagaagaccg ccggatgctg gcggacacgt tgaaccgctt catcgccgaa cagtacgcgt tcccggtgcg cgatcgcatc gcgcagtcgg ccgacgggtt cgatcgcacg atgtggcagc gcttcgccga actcggcgcg gtcggcgcgc tgtttgccga ggccgacggc ggcttcggcg gcacgggctt cgacatcgcc gtggtgttcg aatgcctcgg gcgcgggctc gtcgtcgagc cgttcctcgg cgcgctgctc gcgggccgcg cgctgtcgct cgccggcggc gccgcgcatc gcgagcggct tgccgcgctg atcgacggca gcgcgagcgc cgcgttcgcg cacgacgagc cgggctcgca ctacgaactg acgacggtgc gcacgcgcgc cgagcgtgcc ggcgacggct gggtgctgac gggcgcgaag ggcgtcgtcg accaggccgc gcaggcggcg ttcttcgtcg tcagcgcgcg cgtgtccggt cacgacgacg acgcggccgg catcggcctg ttcgtcgtgc cggccgacgc gccgggcgtg tcgctgcgcg actaccggaa gatcgatggt ggccgtgcgg ccgaagtgcg tttcgggcgc gtcgcgctgc cggccgacgc cgcgctcggc gggccgagtg cgcacgacgg cgaagcgggc gccgcgctgc tcgaacgcgt gctcggctac gggctgctcg cgctgtcggc cgaagcgctc ggcgcgatgg acatcgtgaa ggaatacacg ctcgactacc tgcgcacgcg caagcagttc gggatggcga tcggcagctt ccaggcgctg cagcaccgga tggccgacct gctgctcgaa gtcgagcagg cgcgctcggc ggtgatcaac gcggccgcgc agctcgatgc gccgcgtgcg gcgcgcgagc gcgcgctcgc ggcggccaag tacagcatcg gccggatcgg cacgctggtc gccgaggaaa gcatccagct gcacggcggg atcgggatga cgtgggagct gccgctgtcg cactacgcga agcggctggt gatgatcgat caccagctcg gcgacgagga tcatcatctc gaagggtata tcgcgctgtc gaaacagtaa gtgagtaaga t
Expected Protein Sequence
MAHHHHHHMD FQHTEDRRML ADTLNRFIAE QYAFPVRDRI AQSADGFDRT MWQRFAELGA VGALFAEADG GFGGTGFDIA VVFECLGRGL VVEPFLGALL AGRALSLAGG AAHRERLAAL IDGSASAAFA HDEPGSHYEL TTVRTRAERA GDGWVLTGAK GVVDQAAQAA FFVVSARVSG HDDDAAGIGL FVVPADAPGV SLRDYRKIDG GRAAEVRFGR VALPADAALG GPSAHDGEAG AALLERVLGY GLLALSAEAL GAMDIAKEYT LDYLRTRKQF GMAIGSFQAL QHRMADLLLE VEQARSAVIN AAAQLDAPRA ARERALAAAK YSIGRIGTLV AEESIQLHGG IGMTWELPLS HYAKRLVMID HQLGDEDHHL ARYIALSKQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatt tccagcacac agaagaccgc cggatgctgg cggacacgtt gaaccgcttc atcgccgaac agtacgcgtt cccggtgcgc gatcgcatcg cgcagtcggc cgacgggttc gatcgcacga tgtggcagcg cttcgccgaa ctcggcgcgg tcggcgcgct gtttgccgag gccgacggcg gcttcggcgg cacgggcttc gacatcgccg tggtgttcga atgcctcggg cgcgggctcg tcgtcgagcc gttcctcggc gcgctgctcg cgggccgcgc gctgtcgctc gccggcggcg ccgcgcatcg cgagcggctt gccgcgctga tcgacggcag cgcgagcgcc gcgttcgcgc acgacgagcc gggctcgcac tacgaactga cgacggtgcg cacgcgcgcc gagcgtgccg gcgacggctg ggtgctgacg ggcgcgaagg gcgtcgtcga ccaggccgcg caggcggcgt tcttcgtcgt cagcgcgcgc gtgtccggtc acgacgacga cgcggccggc atcggcctgt tcgtcgtgcc ggccgacgcg ccgggcgtgt cgctgcgcga ctaccggaag atcgatggtg gccgtgcggc cgaagtgcgt ttcgggcgcg tcgcgctgcc ggccgacgcc gcgctcggcg ggccgagtgc gcacgacggc gaagcgggcg ccgcgctgct cgaacgcgtg ctcggctacg ggctgctcgc gctgtcggcc gaagcgctcg gcgcgatgga catcgcgaag gaatacacgc tcgactacct gcgcacgcgc aagcagttcg ggatggcgat cggcagcttc caggcgctgc agcaccggat ggccgacctg ctgctcgaag tcgagcaggc gcgctcggcg gtgatcaacg cggccgcgca gctcgatgcg ccgcgtgcgg cgcgcgagcg cgcgctcgcg gcggccaagt acagcatcgg ccggatcggc acgctggtcg ccgaggaaag catccagctg cacggcggga tcgggatgac gtgggagctg ccgctgtcgc actacgcgaa gcggctggtg atgatcgatc accagctcgg cgacgaggat catcatctcg cgcgctatat cgcgctgtcg aaacagtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t