BuamA.00024.c

Taurine dioxygenase

CENTER ID: BuamA.00024.c
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00024.c.B1.GE39244 Full length( BuamA.00024.c ) 1 308
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.6725
RefSeq: YP_001816237.1
UniProt: B1Z4M1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTSAIRAAS TSTGHRAALP PEAGAQRFDI VPFDAPLGAE VVGIDLSQPL GAADFARVHR AHLDHHVLVF RDQRITPDEH VAFSRRFGPL QIHVLHQFAL AAHPEVLIVS NIVENGRPIG LGDAGHFWHS DLSYKEKPSL GSLLHAQELP AEGGDTLFAN MHLAWDTLPA HLRRAVEGRR AEHTYLARYA ELQARSPWRP NLSAEQIAQV EAVVHPVVRT HPETGRKALF VSEHFTTRIV DLPEDESRAL LDELFAHSVR AEHLYRHHWR AHDLVFWDNR SLMHLAAGTP DHLRRKLYRT TIEGDAPF
NT Sequence
GTGACGACTT CCGCCATCCG CGCCGCATCG ACGTCCACCG GCCATCGGGC CGCGTTGCCG CCGGAAGCCG GCGCGCAGCG ATTCGACATC GTCCCGTTCG ATGCGCCGCT CGGCGCCGAA GTCGTCGGCA TCGACCTGTC GCAACCGCTC GGCGCCGCCG ATTTCGCGCG CGTCCATCGC GCGCATCTCG ACCATCACGT GCTGGTGTTT CGCGACCAGC GCATCACGCC GGACGAACAC GTCGCGTTCA GCCGCCGCTT CGGCCCGCTG CAGATCCATG TGCTGCACCA GTTCGCGCTG GCCGCGCATC CGGAAGTGCT GATCGTGTCG AACATCGTCG AGAACGGCCG GCCGATCGGG CTCGGCGACG CCGGCCATTT CTGGCACTCC GATCTGTCGT ACAAGGAAAA GCCGAGCCTC GGCTCGCTGC TGCATGCGCA GGAGCTGCCG GCCGAAGGCG GCGACACGCT GTTCGCGAAC ATGCATCTCG CGTGGGATAC GCTGCCCGCG CACCTGCGTC GCGCGGTCGA AGGGCGGCGT GCCGAGCATA CGTATCTGGC GCGCTATGCC GAGCTGCAGG CGCGCAGCCC GTGGCGGCCG AACCTGTCGG CCGAGCAGAT CGCGCAAGTC GAGGCGGTGG TGCATCCGGT CGTGCGCACG CATCCGGAAA CGGGCCGCAA GGCGTTGTTC GTCAGCGAGC ATTTCACGAC GCGCATCGTC GATCTGCCCG AAGACGAAAG CCGCGCGCTG CTCGACGAAC TGTTCGCGCA TAGCGTGCGT GCCGAGCATC TGTATCGGCA CCACTGGCGC GCGCACGACC TCGTGTTCTG GGACAACCGC TCGCTGATGC ATCTCGCGGC CGGCACGCCC GACCATCTGC GCCGCAAGCT GTACCGCACG ACGATCGAAG GCGACGCGCC GTTC
Details for BuamA.00024.c.B1.GE39244
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 36kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMV TTSAIRAAST STGHRAALPP EAGAQRFDIV PFDAPLGAEV VGIDLSQPLG AADFARVHRA HLDHHVLVFR DQRITPDEHV AFSRRFGPLQ IHVLHQFALA AHPEVLIVSN IVENGRPIGL GDAGHFWHSD LSYKEKPSLG SLLHAQELPA EGGDTLFANM HLAWDTLPAH LRRAVEGRRA EHTYLARYAE LQARSPWRPN LSAEQIAQVE AVVHPVVRTH PETGRKALFV SEHFTTRIVD LPEDESRALL DELFAHSVRA EHLYRHHWRA HDLVFWDNRS LMHLAAGTPD HLRRKLYRTT IEGDAP
Validated NT Sequence
acggcgcgtc gccttcgatc gtcgtgcggt acagcttgcg gcgcagatgg tcgggcgtgc cggccgcgag atgcatcagc gagcggttgt cccagaacac gaggtcgtgc gcgcgccagt ggtgccgata cagatgctcg gcacgcacgc tatgcgcgaa cagttcgtcg agcagcgcgc ggctttcgtc ttcgggcaga tcgacgatgc gcgtcgtgaa atgctcgctg acgaacaacg ccttgcggcc cgtttccgga tgcgtgcgca cgaccggatg caccaccgcc tcgacttgcg cgatctgctc ggccgacagg ttcggccgcc acgggctgcg cgcctgcagc tcggcatagc gcgccagata cgtatgctcg gcacgccgcc cttcgaccgc gcgacgcagg tgcgcgggca gcgtatccca cgcgagatgc atgttcgcga acagcgtgtc gccgccttcg gccggcagct cctgcgcatg cagcagcgag ccgaggctcg gcttttcctt gtacgacaga tcggagtgcc agaaatggcc ggcgtcgccg agcccgatcg gccggccgtt ctcgacgatg ttcgacacga tcagcacttc cggatgcgcg gccagcgcga actggtgcag cacatggatc tgcagcgggc cgaagcggcg gctgaacgcg acgtgttcgt ccggcgtgat gcgctggtcg cgaaacacca gcacgtgatg gtcgagatgc gcgcgatgga cgcgcgcgaa atcggcggcg ccgagcggtt gcgacaggtc gatgccgacg acttcggcgc cgagcggcgc atcgaacggg acgatgtcga atcgctgcgc gccggcttcc ggcggcaacg cggcccgatg gccggtggac gtcgatgcgg cgcggatggc ggaagtcgtc accatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT TSAIRAASTS TGHRAALPPE AGAQRFDIVP FDAPLGAEVV GIDLSQPLGA ADFARVHRAH LDHHVLVFRD QRITPDEHVA FSRRFGPLQI HVLHQFALAA HPEVLIVSNI VENGRPIGLG DAGHFWHSDL SYKEKPSLGS LLHAQELPAE GGDTLFANMH LAWDTLPAHL RRAVEGRRAE HTYLARYAEL QARSPWRPNL SAEQIAQVEA VVHPVVRTHP ETGRKALFVS EHFTTRIVDL PEDESRALLD ELFAHSVRAE HLYRHHWRAH DLVFWDNRSL MHLAAGTPDH LRRKLYRTTI EGDAPF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacga cttccgccat ccgcgccgca tcgacgtcca ccggccatcg ggccgcgttg ccgccggaag ccggcgcgca gcgattcgac atcgtcccgt tcgatgcgcc gctcggcgcc gaagtcgtcg gcatcgacct gtcgcaaccg ctcggcgccg ccgatttcgc gcgcgtccat cgcgcgcatc tcgaccatca cgtgctggtg tttcgcgacc agcgcatcac gccggacgaa cacgtcgcgt tcagccgccg cttcggcccg ctgcagatcc atgtgctgca ccagttcgcg ctggccgcgc atccggaagt gctgatcgtg tcgaacatcg tcgagaacgg ccggccgatc gggctcggcg acgccggcca tttctggcac tccgatctgt cgtacaagga aaagccgagc ctcggctcgc tgctgcatgc gcaggagctg ccggccgaag gcggcgacac gctgttcgcg aacatgcatc tcgcgtggga tacgctgccc gcgcacctgc gtcgcgcggt cgaagggcgg cgtgccgagc atacgtatct ggcgcgctat gccgagctgc aggcgcgcag cccgtggcgg ccgaacctgt cggccgagca gatcgcgcaa gtcgaggcgg tggtgcatcc ggtcgtgcgc acgcatccgg aaacgggccg caaggcgttg ttcgtcagcg agcatttcac gacgcgcatc gtcgatctgc ccgaagacga aagccgcgcg ctgctcgacg aactgttcgc gcatagcgtg cgtgccgagc atctgtatcg gcaccactgg cgcgcgcacg acctcgtgtt ctgggacaac cgctcgctga tgcatctcgc ggccggcacg cccgaccatc tgcgccgcaa gctgtaccgc acgacgatcg aaggcgacgc gccgttctga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at