BuamA.00024.b

Taurine dioxygenase

CENTER ID: BuamA.00024.b
ORGANISM: Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00024.b.B1.GE39243 Full length( BuamA.00024.b ) 1 317
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuamA.00024.b.B1.PS02548 Full length( BuamA.00024.b ) 1 317

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|398577.6.peg.4041
RefSeq: YP_001810606.1
UniProt: B1Z256

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDVQHAAYP SSHASHAAPH TAAAPLELRQ VAGRIGAEIA GVRLSSTLDD ATFDAIQAAL LRHKVLFFRN QEHLDDAAQE AFARRFGDTV AHPTVPPADG SAHLLELDSA HGARANSWHT DVTFVDAYPK VSILRAVVIP PFGGDTVWAN TATAYAHLPE PLRALADTLW ALHTNAYDYA STHVHADDTQ LKRYREVFTS SVYETEHPVV RVHPETGERT LVLGHFVQKI KGLSSQDSAH LLQVLHEHVT RLENTVRWRW QQGDVAIWDN RATQHYAIND YGDARRVVRR ATVHGDVPVG IDGRRSILLK GPGVTLQ
NT Sequence
ATGTCCGACG TCCAGCATGC CGCTTACCCG TCCTCGCATG CTTCGCACGC CGCGCCGCAC ACGGCCGCCG CCCCGCTTGA GCTGCGCCAG GTCGCCGGCC GGATCGGCGC CGAGATCGCC GGCGTGCGGC TGTCCAGCAC GCTCGACGAC GCGACGTTCG ACGCGATCCA GGCCGCGCTG CTGCGCCACA AGGTGCTGTT CTTCCGCAAT CAGGAGCATC TCGACGACGC CGCGCAGGAA GCATTCGCGC GCCGCTTCGG CGACACCGTC GCGCATCCGA CCGTGCCGCC GGCGGACGGC AGCGCGCACC TGCTCGAACT CGATTCCGCG CACGGCGCGC GCGCCAATTC CTGGCACACC GATGTGACGT TCGTCGACGC GTACCCGAAG GTGTCGATCC TGCGCGCGGT CGTGATTCCG CCGTTCGGCG GCGACACGGT GTGGGCCAAC ACGGCGACCG CGTATGCGCA CCTGCCGGAG CCGCTGCGCG CGCTCGCCGA TACGCTGTGG GCACTGCACA CGAACGCCTA CGACTATGCG TCGACCCACG TGCACGCCGA CGACACGCAA CTGAAACGCT ACCGCGAAGT GTTCACGTCG AGCGTCTACG AAACCGAACA CCCGGTCGTG CGCGTGCATC CGGAGACCGG CGAGCGCACG CTCGTGCTCG GGCATTTCGT GCAGAAGATC AAGGGGTTGT CGTCGCAGGA TTCCGCGCAT CTGCTGCAGG TGCTGCACGA ACACGTGACG CGCCTCGAGA ACACCGTGCG CTGGCGCTGG CAGCAAGGCG ACGTCGCGAT CTGGGACAAC CGCGCGACGC AGCACTATGC GATCAACGAT TACGGCGATG CGCGCCGCGT CGTGCGCCGC GCGACGGTTC ACGGCGATGT GCCGGTCGGG ATCGACGGCC GCCGCAGCAT CTTGCTGAAG GGGCCGGGGG TGACATTGCA G
Details for BuamA.00024.b.B1.GE39243
HARVESTED ON: 6/10/2015
SEQUENCED ON: 6/5/2015
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMS DVQHAAYPSS HASHAAPHTA AAPLELRQVA GRIGAEIAGV RLSSTLDDAT FDAIQAALLR HKVLFFRNQE HLDDAAQEAF ARRFGDTVAH PTVPPADGSA HLLELDSAHG ARANSWHTDV TFVDAYPKVS ILRAVVIPPF GGDTVWANTA TAYAHLPEPL RALADTLWAL HTNAYDYAST HVHADDTQLK RYREVFTSSV YETEHPVVRV HPETGERTLV LGHFVQKIKG LSSQDSAHLL QVLHEHVTRL ENTVRWRWQQ GDVAIWDNRA TQHYAINDYG DARRVVRRAT VHGDVPVGID GRRSILLKGP GVTL
Validated NT Sequence
tgcaatgtca cccccggccc cttcagcaag atgctgcggc ggccgtcgat cccgaccggc acatcgccgt gaaccgtcgc gcggcgcacg acgcggcgcg catcgccgta atcgttgatc gcatagtgct gcgtcgcgcg gttgtcccag atcgcgacgt cgccttgctg ccagcgccag cgcacggtgt tctcgaggcg cgtcacgtgt tcgtgcagca cctgcagcag atgcgcggaa tcctgcgacg acaacccctt gatcttctgc acgaaatgcc cgagcacgag cgtgcgctcg ccggtctccg gatgcacgcg cacgaccggg tgttcggttt cgtagacgct cgacgtgaac acttcgcggt agcgtttcag ttgcgtgtcg tcggcgtgca cgtgggtcga cgcatagtcg taggcgttcg tgtgcagtgc ccacagcgta tcggcgagcg cgcgcagcgg ctccggcagg tgcgcatacg cggtcgccgt gttggcccac accgtgtcgc cgccgaacgg cggaatcacg accgcgcgca ggatcgacac cttcgggtac gcgtcgacga acgtcacatc ggtgtgccag gaattggcgc gcgcgccgtg cgcggaatcg agttcgagca ggtgcgcgct gccgtccgcc ggcggcacgg tcggatgcgc gacggtgtcg ccgaagcggc gcgcgaatgc ttcctgcgcg gcgtcgtcga gatgctcctg attgcggaag aacagcacct tgtggcgcag cagcgcggcc tggatcgcgt cgaacgtcgc gtcgtcgagc gtgctggaca gccgcacgcc ggcgatctcg gcgccgatcc ggccggcgac ctggcgcagc tcaagcgggg cggcggccgt gtgcggcgcg gcgtgcgaag catgcgagga cgggtaagcg gcatgctgga cgtcggacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMS DVQHAAYPSS HASHAAPHTA AAPLELRQVA GRIGAEIAGV RLSSTLDDAT FDAIQAALLR HKVLFFRNQE HLDDAAQEAF ARRFGDTVAH PTVPPADGSA HLLELDSAHG ARANSWHTDV TFVDAYPKVS ILRAVVIPPF GGDTVWANTA TAYAHLPEPL RALADTLWAL HTNAYDYAST HVHADDTQLK RYREVFTSSV YETEHPVVRV HPETGERTLV LGHFVQKIKG LSSQDSAHLL QVLHEHVTRL ENTVRWRWQQ GDVAIWDNRA TQHYAINDYG DARRVVRRAT VHGDVPVGID GRRSILLKGP GVTLQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg acgtccagca tgccgcttac ccgtcctcgc atgcttcgca cgccgcgccg cacacggccg ccgccccgct tgagctgcgc caggtcgccg gccggatcgg cgccgagatc gccggcgtgc ggctgtccag cacgctcgac gacgcgacgt tcgacgcgat ccaggccgcg ctgctgcgcc acaaggtgct gttcttccgc aatcaggagc atctcgacga cgccgcgcag gaagcattcg cgcgccgctt cggcgacacc gtcgcgcatc cgaccgtgcc gccggcggac ggcagcgcgc acctgctcga actcgattcc gcgcacggcg cgcgcgccaa ttcctggcac accgatgtga cgttcgtcga cgcgtacccg aaggtgtcga tcctgcgcgc ggtcgtgatt ccgccgttcg gcggcgacac ggtgtgggcc aacacggcga ccgcgtatgc gcacctgccg gagccgctgc gcgcgctcgc cgatacgctg tgggcactgc acacgaacgc ctacgactat gcgtcgaccc acgtgcacgc cgacgacacg caactgaaac gctaccgcga agtgttcacg tcgagcgtct acgaaaccga acacccggtc gtgcgcgtgc atccggagac cggcgagcgc acgctcgtgc tcgggcattt cgtgcagaag atcaaggggt tgtcgtcgca ggattccgcg catctgctgc aggtgctgca cgaacacgtg acgcgcctcg agaacaccgt gcgctggcgc tggcagcaag gcgacgtcgc gatctgggac aaccgcgcga cgcagcacta tgcgatcaac gattacggcg atgcgcgccg cgtcgtgcgc cgcgcgacgg ttcacggcga tgtgccggtc gggatcgacg gccgccgcag catcttgctg aaggggccgg gggtgacatt gcagtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuamA.00024.b.B1.PS02548
PURIFICATION DATe: 12/9/2015
CONCENTRATION: 54.84mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: SEC buffer
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS DVQHAAYPSS HASHAAPHTA AAPLELRQVA GRIGAEIAGV RLSSTLDDAT FDAIQAALLR HKVLFFRNQE HLDDAAQEAF ARRFGDTVAH PTVPPADGSA HLLELDSAHG ARANSWHTDV TFVDAYPKVS ILRAVVIPPF GGDTVWANTA TAYAHLPEPL RALADTLWAL HTNAYDYAST HVHADDTQLK RYREVFTSSV YETEHPVVRV HPETGERTLV LGHFVQKIKG LSSQDSAHLL QVLHEHVTRL ENTVRWRWQQ GDVAIWDNRA TQHYAINDYG DARRVVRRAT VHGDVPVGID GRRSILLKGP GVTL
Validated NT Sequence
tgcaatgtca cccccggccc cttcagcaag atgctgcggc ggccgtcgat cccgaccggc acatcgccgt gaaccgtcgc gcggcgcacg acgcggcgcg catcgccgta atcgttgatc gcatagtgct gcgtcgcgcg gttgtcccag atcgcgacgt cgccttgctg ccagcgccag cgcacggtgt tctcgaggcg cgtcacgtgt tcgtgcagca cctgcagcag atgcgcggaa tcctgcgacg acaacccctt gatcttctgc acgaaatgcc cgagcacgag cgtgcgctcg ccggtctccg gatgcacgcg cacgaccggg tgttcggttt cgtagacgct cgacgtgaac acttcgcggt agcgtttcag ttgcgtgtcg tcggcgtgca cgtgggtcga cgcatagtcg taggcgttcg tgtgcagtgc ccacagcgta tcggcgagcg cgcgcagcgg ctccggcagg tgcgcatacg cggtcgccgt gttggcccac accgtgtcgc cgccgaacgg cggaatcacg accgcgcgca ggatcgacac cttcgggtac gcgtcgacga acgtcacatc ggtgtgccag gaattggcgc gcgcgccgtg cgcggaatcg agttcgagca ggtgcgcgct gccgtccgcc ggcggcacgg tcggatgcgc gacggtgtcg ccgaagcggc gcgcgaatgc ttcctgcgcg gcgtcgtcga gatgctcctg attgcggaag aacagcacct tgtggcgcag cagcgcggcc tggatcgcgt cgaacgtcgc gtcgtcgagc gtgctggaca gccgcacgcc ggcgatctcg gcgccgatcc ggccggcgac ctggcgcagc tcaagcgggg cggcggccgt gtgcggcgcg gcgtgcgaag catgcgagga cgggtaagcg gcatgctgga cgtcggacat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMS DVQHAAYPSS HASHAAPHTA AAPLELRQVA GRIGAEIAGV RLSSTLDDAT FDAIQAALLR HKVLFFRNQE HLDDAAQEAF ARRFGDTVAH PTVPPADGSA HLLELDSAHG ARANSWHTDV TFVDAYPKVS ILRAVVIPPF GGDTVWANTA TAYAHLPEPL RALADTLWAL HTNAYDYAST HVHADDTQLK RYREVFTSSV YETEHPVVRV HPETGERTLV LGHFVQKIKG LSSQDSAHLL QVLHEHVTRL ENTVRWRWQQ GDVAIWDNRA TQHYAINDYG DARRVVRRAT VHGDVPVGID GRRSILLKGP GVTLQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG ACGTCCAGCA TGCCGCTTAC CCGTCCTCGC ATGCTTCGCA CGCCGCGCCG CACACGGCCG CCGCCCCGCT TGAGCTGCGC CAGGTCGCCG GCCGGATCGG CGCCGAGATC GCCGGCGTGC GGCTGTCCAG CACGCTCGAC GACGCGACGT TCGACGCGAT CCAGGCCGCG CTGCTGCGCC ACAAGGTGCT GTTCTTCCGC AATCAGGAGC ATCTCGACGA CGCCGCGCAG GAAGCATTCG CGCGCCGCTT CGGCGACACC GTCGCGCATC CGACCGTGCC GCCGGCGGAC GGCAGCGCGC ACCTGCTCGA ACTCGATTCC GCGCACGGCG CGCGCGCCAA TTCCTGGCAC ACCGATGTGA CGTTCGTCGA CGCGTACCCG AAGGTGTCGA TCCTGCGCGC GGTCGTGATT CCGCCGTTCG GCGGCGACAC GGTGTGGGCC AACACGGCGA CCGCGTATGC GCACCTGCCG GAGCCGCTGC GCGCGCTCGC CGATACGCTG TGGGCACTGC ACACGAACGC CTACGACTAT GCGTCGACCC ACGTGCACGC CGACGACACG CAACTGAAAC GCTACCGCGA AGTGTTCACG TCGAGCGTCT ACGAAACCGA ACACCCGGTC GTGCGCGTGC ATCCGGAGAC CGGCGAGCGC ACGCTCGTGC TCGGGCATTT CGTGCAGAAG ATCAAGGGGT TGTCGTCGCA GGATTCCGCG CATCTGCTGC AGGTGCTGCA CGAACACGTG ACGCGCCTCG AGAACACCGT GCGCTGGCGC TGGCAGCAAG GCGACGTCGC GATCTGGGAC AACCGCGCGA CGCAGCACTA TGCGATCAAC GATTACGGCG ATGCGCGCCG CGTCGTGCGC CGCGCGACGG TTCACGGCGA TGTGCCGGTC GGGATCGACG GCCGCCGCAG CATCTTGCTG AAGGGGCCGG GGGTGACATT GCAGTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT