BuamA.00010.o
Short-chain dehydrogenase/reductase SDR
| CENTER ID: | BuamA.00010.o |
| ORGANISM: | Burkholderia ambifaria MC40-6 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00010.o.B1.GE39922 | Full length( BuamA.00010.o ) | 1 | 259 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuamA.00010.o.B1.PS38204 | Full length( BuamA.00010.o ) | 1 | 259 |
Structures
5VPS
DEPOSITED: 5/5/2017
DETERMINATION: XRay
CLONE: BuamA.00010.o.B1.GE39922
PROTEIN: BuamA.00010.o.B1.PS38204
Publications by SSGCID
Cautionary Tale of Using Tris(alkyl)phosphine Reducing Agents with NAD+-Dependent Enzymes
Edwards TE, Mayclin SJ, Patel SM, Smith TG, Morton M, Stiers KM, Seravalli J, Tanner JJ, Becker DF
Biochemistry - 2020
volume 59, issue 36, pages 3285-3289
PMID: 32841567; PMCID: PMC8528484
PDB:
5VPS
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_129079 |
| PATRIC ID: | fig|398577.6.peg.2265 |
| RefSeq: | YP_001808903.1 |
| UniProt: | B1YU47 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLGIAGKTA LVCAASKGLG RGCAEALAAE GVNLVIVART RDTLEETAEE IRAASNVSVT AVACDITTPD GRAAALAACP QPDILVNNAG GPPPGDFRDF SHDDWIRALE ANMLTPIELI RATVDGMIGR GFGRIVNITS SAVKAPIDVL ALSNGARSGL TGFVAGLSRK VAGQGVTINN LLPGLFDTDR IATTLAASAK AQGVTVDELR ARRAKEIPAG RLGTRAEFGA ACAFLCSVHA GYITGQNWLL DGGAYPGTF
NT Sequence
ATGGATCTCG GTATCGCAGG AAAGACCGCC CTCGTGTGCG CGGCGAGCAA GGGCCTCGGG CGCGGCTGCG CGGAAGCGCT GGCCGCCGAG GGCGTGAATC TCGTGATCGT CGCGCGCACG CGCGACACGC TGGAGGAAAC CGCCGAGGAG ATCCGCGCCG CGTCGAACGT GTCGGTCACC GCCGTCGCGT GCGACATCAC GACGCCCGAC GGGCGCGCGG CTGCGCTCGC CGCCTGCCCG CAGCCGGACA TTCTCGTGAA CAATGCGGGC GGCCCGCCGC CTGGCGACTT CCGCGATTTC TCGCACGACG ACTGGATCCG CGCGCTCGAG GCGAACATGC TGACGCCGAT CGAGCTGATC CGCGCGACCG TCGACGGGAT GATCGGGCGC GGCTTCGGCC GGATCGTCAA TATCACGAGT TCGGCGGTGA AGGCGCCGAT CGACGTGCTC GCGCTGTCGA ACGGCGCGCG CTCGGGGCTG ACCGGCTTCG TCGCGGGGCT GTCGCGCAAG GTCGCCGGCC AGGGCGTGAC CATCAACAAC CTGCTGCCGG GGCTGTTCGA CACCGACCGG ATCGCGACGA CGCTCGCCGC GTCGGCGAAG GCGCAGGGCG TGACGGTCGA CGAACTGCGC GCGCGGCGCG CGAAGGAGAT CCCGGCCGGG CGCCTCGGCA CGCGCGCCGA ATTCGGCGCC GCGTGCGCGT TCCTCTGCAG CGTGCATGCC GGCTATATCA CCGGGCAGAA CTGGCTGCTC GACGGCGGCG CGTATCCGGG CACGTTC
Details for BuamA.00010.o.B1.GE39922
| HARVESTED ON: | 2/26/2016 |
| SEQUENCED ON: | 3/1/2016 |
| EXPECTED MW: | 28kDa |
| OBSERVED MW: | 28kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | High Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMD LGIAGKTALV CAASKGLGRG CAEALAAEGV NLVIVARTRD TLEETAEEIR AASNVSVTAV ACDITTPDGR AAALAACPQP DILVNNAGGP PPGDFRDFSH DDWIRALEAN MLTPIELIRA TVDGMIGRGF GRIVNITSSA VKAPIDVLAL SNGARSGLTG FVAGLSRKVA GQGVTINNLL PGLFDTDRIA TTLAASAKAQ GVTVDELRAR RAKEIPAGRL GTRAEFGAAC AFLCSVHAGY ITGQNWLLXX GAYPGTF
Validated NT Sequence
atcttactca cttagaacgt gcccggatac gcgccnnnnn cgagcagcca gttctgcccg gtgatatagc cggcatgcac gctgcagagg aacgcgcacg cggcgccgaa ttcggcgcgc gtgccgaggc gcccggccgg gatctccttc gcgcgccgcg cgcgcagttc gtcgaccgtc acgccctgcg ccttcgccga cgcggcgagc gtcgtcgcga tccggtcggt gtcgaacagc cccggcagca ggttgttgat ggtcacgccc tggccggcga ccttgcgcga cagccccgcg acgaagccgg tcagccccga gcgcgcgccg ttcgacagcg cgagcacgtc gatcggcgcc ttcaccgccg aactcgtgat attgacgatc cggccgaagc cgcgcccgat catcccgtcg acggtcgcgc ggatcagctc gatcggcgtc agcatgttcg cctcgagcgc gcggatccag tcgtcgtgcg agaaatcgcg gaagtcgcca ggcggcgggc cgcccgcatt gttcacgaga atgtccggct gcgggcaggc ggcgagcgca gccgcgcgcc cgtcgggcgt cgtgatgtcg cacgcgacgg cggtgaccga cacgttcgac gcggcgcgga tctcctcggc ggtttcctcc agcgtgtcgc gcgtgcgcgc gacgatcacg agattcacgc cctcggcggc cagcgcttcc gcgcagccgc gcccgaggcc cttgctcgcc gcgcacacga gggcggtctt tcctgcgata ccgagatcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMD LGIAGKTALV CAASKGLGRG CAEALAAEGV NLVIVARTRD TLEETAEEIR AASNVSVTAV ACDITTPDGR AAALAACPQP DILVNNAGGP PPGDFRDFSH DDWIRALEAN MLTPIELIRA TVDGMIGRGF GRIVNITSSA VKAPIDVLAL SNGARSGLTG FVAGLSRKVA GQGVTINNLL PGLFDTDRIA TTLAASAKAQ GVTVDELRAR RAKEIPAGRL GTRAEFGAAC AFLCSVHAGY ITGQNWLLDG GAYPGTF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggatc tcggtatcgc aggaaagacc gccctcgtgt gcgcggcgag caagggcctc gggcgcggct gcgcggaagc gctggccgcc gagggcgtga atctcgtgat cgtcgcgcgc acgcgcgaca cgctggagga aaccgccgag gagatccgcg ccgcgtcgaa cgtgtcggtc accgccgtcg cgtgcgacat cacgacgccc gacgggcgcg cggctgcgct cgccgcctgc ccgcagccgg acattctcgt gaacaatgcg ggcggcccgc cgcctggcga cttccgcgat ttctcgcacg acgactggat ccgcgcgctc gaggcgaaca tgctgacgcc gatcgagctg atccgcgcga ccgtcgacgg gatgatcggg cgcggcttcg gccggatcgt caatatcacg agttcggcgg tgaaggcgcc gatcgacgtg ctcgcgctgt cgaacggcgc gcgctcgggg ctgaccggct tcgtcgcggg gctgtcgcgc aaggtcgccg gccagggcgt gaccatcaac aacctgctgc cggggctgtt cgacaccgac cggatcgcga cgacgctcgc cgcgtcggcg aaggcgcagg gcgtgacggt cgacgaactg cgcgcgcggc gcgcgaagga gatcccggcc gggcgcctcg gcacgcgcgc cgaattcggc gccgcgtgcg cgttcctctg cagcgtgcat gccggctata tcaccgggca gaactggctg ctcgacggcg gcgcgtatcc gggcacgttc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuamA.00010.o.B1.PS38204
| PURIFICATION DATe: | 2/15/2017 |
| CONCENTRATION: | 16.34mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMD LGIAGKTALV CAASKGLGRG CAEALAAEGV NLVIVARTRD TLEETAEEIR AASNVSVTAV ACDITTPDGR AAALAACPQP DILVNNAGGP PPGDFRDFSH DDWIRALEAN MLTPIELIRA TVDGMIGRGF GRIVNITSSA VKAPIDVLAL SNGARSGLTG FVAGLSRKVA GQGVTINNLL PGLFDTDRIA TTLAASAKAQ GVTVDELRAR RAKEIPAGRL GTRAEFGAAC AFLCSVHAGY ITGQNWLLXX GAYPGTF
Validated NT Sequence
atcttactca cttagaacgt gcccggatac gcgccnnnnn cgagcagcca gttctgcccg gtgatatagc cggcatgcac gctgcagagg aacgcgcacg cggcgccgaa ttcggcgcgc gtgccgaggc gcccggccgg gatctccttc gcgcgccgcg cgcgcagttc gtcgaccgtc acgccctgcg ccttcgccga cgcggcgagc gtcgtcgcga tccggtcggt gtcgaacagc cccggcagca ggttgttgat ggtcacgccc tggccggcga ccttgcgcga cagccccgcg acgaagccgg tcagccccga gcgcgcgccg ttcgacagcg cgagcacgtc gatcggcgcc ttcaccgccg aactcgtgat attgacgatc cggccgaagc cgcgcccgat catcccgtcg acggtcgcgc ggatcagctc gatcggcgtc agcatgttcg cctcgagcgc gcggatccag tcgtcgtgcg agaaatcgcg gaagtcgcca ggcggcgggc cgcccgcatt gttcacgaga atgtccggct gcgggcaggc ggcgagcgca gccgcgcgcc cgtcgggcgt cgtgatgtcg cacgcgacgg cggtgaccga cacgttcgac gcggcgcgga tctcctcggc ggtttcctcc agcgtgtcgc gcgtgcgcgc gacgatcacg agattcacgc cctcggcggc cagcgcttcc gcgcagccgc gcccgaggcc cttgctcgcc gcgcacacga gggcggtctt tcctgcgata ccgagatcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMD LGIAGKTALV CAASKGLGRG CAEALAAEGV NLVIVARTRD TLEETAEEIR AASNVSVTAV ACDITTPDGR AAALAACPQP DILVNNAGGP PPGDFRDFSH DDWIRALEAN MLTPIELIRA TVDGMIGRGF GRIVNITSSA VKAPIDVLAL SNGARSGLTG FVAGLSRKVA GQGVTINNLL PGLFDTDRIA TTLAASAKAQ GVTVDELRAR RAKEIPAGRL GTRAEFGAAC AFLCSVHAGY ITGQNWLLDG GAYPGTF
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGATC TCGGTATCGC AGGAAAGACC GCCCTCGTGT GCGCGGCGAG CAAGGGCCTC GGGCGCGGCT GCGCGGAAGC GCTGGCCGCC GAGGGCGTGA ATCTCGTGAT CGTCGCGCGC ACGCGCGACA CGCTGGAGGA AACCGCCGAG GAGATCCGCG CCGCGTCGAA CGTGTCGGTC ACCGCCGTCG CGTGCGACAT CACGACGCCC GACGGGCGCG CGGCTGCGCT CGCCGCCTGC CCGCAGCCGG ACATTCTCGT GAACAATGCG GGCGGCCCGC CGCCTGGCGA CTTCCGCGAT TTCTCGCACG ACGACTGGAT CCGCGCGCTC GAGGCGAACA TGCTGACGCC GATCGAGCTG ATCCGCGCGA CCGTCGACGG GATGATCGGG CGCGGCTTCG GCCGGATCGT CAATATCACG AGTTCGGCGG TGAAGGCGCC GATCGACGTG CTCGCGCTGT CGAACGGCGC GCGCTCGGGG CTGACCGGCT TCGTCGCGGG GCTGTCGCGC AAGGTCGCCG GCCAGGGCGT GACCATCAAC AACCTGCTGC CGGGGCTGTT CGACACCGAC CGGATCGCGA CGACGCTCGC CGCGTCGGCG AAGGCGCAGG GCGTGACGGT CGACGAACTG CGCGCGCGGC GCGCGAAGGA GATCCCGGCC GGGCGCCTCG GCACGCGCGC CGAATTCGGC GCCGCGTGCG CGTTCCTCTG CAGCGTGCAT GCCGGCTATA TCACCGGGCA GAACTGGCTG CTCGACGGCG GCGCGTATCC GGGCACGTTC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT