SSGCID: Target Page

BuamA.00009.a

Kynureninase

CENTER ID:	BuamA.00009.a
ORGANISM:	Burkholderia ambifaria MC40-6
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00009.a.B1.GE39238	Full length( BuamA.00009.a )		1	417

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuamA.00009.a.B1.PW37797	Full length( BuamA.00009.a )		1	417

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|398577.6.peg.2569
RefSeq:	YP_001809191.1
UniProt:	B1YVH1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MIKTREDALA LDRDDPLAAL RDQFALPDGV IYLDGNSLGA QPRASAARAQ QVIGAEWGEG LIRSWNTAGW FALPRRLGDK LATLIGGAPG ETVVTDTISI NLFKLLSAML RHQAERAPER RVIVSERSNF PTDLYIAQGL IEQLGGGYEL RLIDDPADLP DALGADTAVA MITHVNYRTG YMHDMPAVTQ LVHDAGALML WDLAHSAGAV PVDLNGAHAD GAVGCTYKYL NGGPGSPAFV WVPQRHHAHF SQPLSGWWGH RAPFAMQPGF APDPGIARFL CGTQPIVSMS MVECGLDVFL QTDMHAIRRK SLALTDAFIA LVEARCAGLS LKLVTPRAHH QRGSQASFEH PHGYEVMQAL IARGVIGDYR EPYVLRFGFT PLYTRFADVW DAVETLRDIL ATDAWKAPEF AERGAVT

NT Sequence

ATGATCAAGA CCCGTGAAGA CGCGCTCGCC CTCGACCGCG ACGACCCGCT CGCCGCGCTG CGCGACCAGT TCGCGCTGCC CGACGGCGTG ATCTATCTCG ACGGCAACTC GCTCGGCGCG CAGCCGCGCG CATCCGCCGC CCGCGCGCAA CAGGTGATCG GCGCCGAATG GGGCGAAGGC CTGATCCGCA GCTGGAACAC CGCCGGCTGG TTCGCGCTGC CGCGCCGCCT CGGCGACAAG CTCGCGACGC TGATCGGCGG CGCACCGGGC GAGACCGTCG TGACCGACAC CATCTCGATC AACCTGTTCA AGCTGCTGTC GGCAATGCTG CGTCACCAGG CCGAGCGCGC GCCGGAGCGC CGCGTGATCG TGTCGGAGCG CTCGAACTTC CCGACCGACC TGTACATCGC GCAAGGGCTG ATCGAGCAGC TCGGCGGCGG CTACGAGCTG CGCCTGATCG ACGATCCGGC CGACCTGCCC GACGCGCTCG GCGCGGACAC GGCCGTCGCG ATGATCACGC ACGTGAACTA CCGCACCGGC TACATGCACG ACATGCCGGC CGTCACGCAG CTCGTGCACG ACGCGGGCGC GCTGATGCTG TGGGATCTCG CGCACTCGGC CGGCGCGGTG CCGGTCGACC TGAACGGCGC GCATGCGGAC GGCGCGGTCG GTTGCACGTA CAAGTACCTG AACGGCGGCC CCGGTTCGCC GGCGTTCGTG TGGGTGCCGC AGCGCCATCA CGCGCATTTC TCGCAACCGC TGTCGGGCTG GTGGGGCCAC CGCGCACCGT TCGCGATGCA GCCGGGCTTC GCGCCCGATC CGGGCATCGC GCGCTTCCTG TGCGGCACGC AGCCGATCGT GTCGATGTCG ATGGTCGAAT GCGGGCTCGA CGTGTTCCTG CAGACCGACA TGCACGCGAT CCGCCGCAAG TCGCTCGCGC TCACCGATGC GTTCATCGCA CTCGTCGAAG CGCGCTGCGC GGGCCTGTCG CTGAAGCTCG TCACGCCGCG CGCGCATCAC CAGCGCGGCT CGCAGGCGAG CTTCGAGCAT CCGCACGGCT ACGAGGTGAT GCAGGCGCTG ATCGCGCGCG GCGTGATCGG CGACTATCGC GAGCCGTACG TGCTGCGCTT CGGCTTCACG CCGCTCTACA CGCGCTTCGC CGACGTGTGG GATGCCGTCG AGACGCTGCG CGACATCCTG GCCACCGATG CGTGGAAGGC ACCCGAGTTC GCCGAGCGCG GCGCGGTGAC C

Details for BuamA.00009.a.B1.GE39238

HARVESTED ON:	6/10/2015
SEQUENCED ON:	6/5/2015
EXPECTED MW:	46kDa
OBSERVED MW:	44kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMI KTREDALALD RDDPLAALRD QFALPDGVIY LDGNSLGAQP RASAARAQQV IGAEWGEGLI RSWNTAGWFA LPRRLGDKLA TLIGGAPGET VVTDTISINL FKLLSAMLRH QAERAPERRV IVSERSNFPT DLYIAQGLIE QLGGGYELRL IDDPADLPDA LGAETAVAMI THVNYRTGYM HDMPAVTQLV HDAGALMLWD LAHSAGAVPV DLNGAHADGA VGCTYKYLNG GPGSPAFVWV PQRHHAHFSQ PLSGWWGHRA PFAMQPGFAP DPGIARFLCG TQPIVSMSMV ECGLDVFLQT DMHAIRRKSL ALTDAFIALV EARCAGLSLK LVTPRAHHQR GSQASFEHPH GYEVMQALIA RGVIGDYREP YVLRFGFTPL YTRFADVWDA VETLRDILAT DAWKAP

Validated NT Sequence

tcgggtgcct tccacgcatc ggtggccagg atgtcgcgca gcgtctcgac ggcatcccac acgtcggcga agcgcgtgta gagcggcgtg aagccgaagc gcagcacgta cggctcgcga tagtcgccga tcacgccgcg cgcgatcagc gcctgcatca cctcgtagcc gtgcggatgc tcgaagctcg cctgcgagcc gcgctggtga tgcgcgcgcg gcgtgacgag cttcagcgac aggcccgcgc agcgcgcttc gacgagtgcg atgaacgcat cggtgagcgc gagcgacttg cggcggatcg cgtgcatgtc ggtctgcagg aacacgtcga gcccgcattc gaccatcgac atcgacacga tcggctgcgt gccgcacagg aagcgcgcga tgcccggatc gggcgcgaag cccggctgca tcgcgaacgg tgcgcggtgg ccccaccagc ccgacagcgg ttgcgagaaa tgcgcgtgat ggcgctgcgg cacccacacg aacgccggcg aaccggggcc gccgttcagg tacttgtacg tgcaaccgac cgcgccgtcc gcatgcgcgc cgttcaggtc gaccggcacc gcgccggccg agtgcgcgag atcccacagc atcagcgcgc ccgcgtcgtg cacgagctgc gtgacggccg gcatgtcgtg catgtagccg gtgcggtagt tcacgtgcgt gatcatcgcg acggccgttt ccgcgccgag cgcgtcgggc aggtcggccg gatcgtcgat caggcgcagc tcgtagccgc cgccgagctg ctcgatcagc ccttgcgcga tgtacaggtc ggtcgggaag ttcgagcgct ccgacacgat cacgcggcgc tccggcgcgc gctcggcctg gtgacgcagc attgccgaca gcagcttgaa caggttgatc gagatggtgt cggtcacgac ggtctcgccc ggtgcgccgc cgatcagcgt cgcgagcttg tcgccgaggc ggcgcggcag cgcgaaccag ccggcggtgt tccagctgcg gatcaggcct tcgccccatt cggcgccgat cacctgttgc gcgcgggcgg cggatgcgcg cggctgcgcg ccgagcgagt tgccgtcgag atagatcacg ccgtcgggca gcgcgaactg gtcgcgcagc gcggcgagcg ggtcgtcgcg gtcgagggcg agcgcgtctt cacgggtctt gatcatatgg tggtggtggt ggtgagccat

Expected Protein Sequence

MAHHHHHHMI KTREDALALD RDDPLAALRD QFALPDGVIY LDGNSLGAQP RASAARAQQV IGAEWGEGLI RSWNTAGWFA LPRRLGDKLA TLIGGAPGET VVTDTISINL FKLLSAMLRH QAERAPERRV IVSERSNFPT DLYIAQGLIE QLGGGYELRL IDDPADLPDA LGADTAVAMI THVNYRTGYM HDMPAVTQLV HDAGALMLWD LAHSAGAVPV DLNGAHADGA VGCTYKYLNG GPGSPAFVWV PQRHHAHFSQ PLSGWWGHRA PFAMQPGFAP DPGIARFLCG TQPIVSMSMV ECGLDVFLQT DMHAIRRKSL ALTDAFIALV EARCAGLSLK LVTPRAHHQR GSQASFEHPH GYEVMQALIA RGVIGDYREP YVLRFGFTPL YTRFADVWDA VETLRDILAT DAWKAPEFAE RGAVT

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatca agacccgtga agacgcgctc gccctcgacc gcgacgaccc gctcgccgcg ctgcgcgacc agttcgcgct gcccgacggc gtgatctatc tcgacggcaa ctcgctcggc gcgcagccgc gcgcatccgc cgcccgcgcg caacaggtga tcggcgccga atggggcgaa ggcctgatcc gcagctggaa caccgccggc tggttcgcgc tgccgcgccg cctcggcgac aagctcgcga cgctgatcgg cggcgcaccg ggcgagaccg tcgtgaccga caccatctcg atcaacctgt tcaagctgct gtcggcaatg ctgcgtcacc aggccgagcg cgcgccggag cgccgcgtga tcgtgtcgga gcgctcgaac ttcccgaccg acctgtacat cgcgcaaggg ctgatcgagc agctcggcgg cggctacgag ctgcgcctga tcgacgatcc ggccgacctg cccgacgcgc tcggcgcgga cacggccgtc gcgatgatca cgcacgtgaa ctaccgcacc ggctacatgc acgacatgcc ggccgtcacg cagctcgtgc acgacgcggg cgcgctgatg ctgtgggatc tcgcgcactc ggccggcgcg gtgccggtcg acctgaacgg cgcgcatgcg gacggcgcgg tcggttgcac gtacaagtac ctgaacggcg gccccggttc gccggcgttc gtgtgggtgc cgcagcgcca tcacgcgcat ttctcgcaac cgctgtcggg ctggtggggc caccgcgcac cgttcgcgat gcagccgggc ttcgcgcccg atccgggcat cgcgcgcttc ctgtgcggca cgcagccgat cgtgtcgatg tcgatggtcg aatgcgggct cgacgtgttc ctgcagaccg acatgcacgc gatccgccgc aagtcgctcg cgctcaccga tgcgttcatc gcactcgtcg aagcgcgctg cgcgggcctg tcgctgaagc tcgtcacgcc gcgcgcgcat caccagcgcg gctcgcaggc gagcttcgag catccgcacg gctacgaggt gatgcaggcg ctgatcgcgc gcggcgtgat cggcgactat cgcgagccgt acgtgctgcg cttcggcttc acgccgctct acacgcgctt cgccgacgtg tgggatgccg tcgagacgct gcgcgacatc ctggccaccg atgcgtggaa ggcacccgag ttcgccgagc gcggcgcggt gacctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat

Details for BuamA.00009.a.B1.PW37797

PURIFICATION DATe:	10/13/2015
CONCENTRATION:	37mg/ml
OBSERVED MW:	45kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	10
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMI KTREDALALD RDDPLAALRD QFALPDGVIY LDGNSLGAQP RASAARAQQV IGAEWGEGLI RSWNTAGWFA LPRRLGDKLA TLIGGAPGET VVTDTISINL FKLLSAMLRH QAERAPERRV IVSERSNFPT DLYIAQGLIE QLGGGYELRL IDDPADLPDA LGADTAVAMI THVNYRTGYM HDMPAVTQLV HDAGALMLWD LAHSAGAVPV DLNGAHADGA VGCTYKYLNG GPGSPAFVWV PQRHHAHFSQ PLSGWWGHRA PFAMQPGFAP DPGIARFLCG TQPIVSMSMV ECGLDVFLQT DMHAIRRKSL ALTDAFIALV EARCAGLSLK LVTPRAHHQR GSQASFEHPH GYEVMQALIA RGVIGDYREP YVLRFGFTPL YTRFADVWDA VETLRDILAT DAWKAPEFAE RGAVT

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATCA AGACCCGTGA AGACGCGCTC GCCCTCGACC GCGACGACCC GCTCGCCGCG CTGCGCGACC AGTTCGCGCT GCCCGACGGC GTGATCTATC TCGACGGCAA CTCGCTCGGC GCGCAGCCGC GCGCATCCGC CGCCCGCGCG CAACAGGTGA TCGGCGCCGA ATGGGGCGAA GGCCTGATCC GCAGCTGGAA CACCGCCGGC TGGTTCGCGC TGCCGCGCCG CCTCGGCGAC AAGCTCGCGA CGCTGATCGG CGGCGCACCG GGCGAGACCG TCGTGACCGA CACCATCTCG ATCAACCTGT TCAAGCTGCT GTCGGCAATG CTGCGTCACC AGGCCGAGCG CGCGCCGGAG CGCCGCGTGA TCGTGTCGGA GCGCTCGAAC TTCCCGACCG ACCTGTACAT CGCGCAAGGG CTGATCGAGC AGCTCGGCGG CGGCTACGAG CTGCGCCTGA TCGACGATCC GGCCGACCTG CCCGACGCGC TCGGCGCGGA CACGGCCGTC GCGATGATCA CGCACGTGAA CTACCGCACC GGCTACATGC ACGACATGCC GGCCGTCACG CAGCTCGTGC ACGACGCGGG CGCGCTGATG CTGTGGGATC TCGCGCACTC GGCCGGCGCG GTGCCGGTCG ACCTGAACGG CGCGCATGCG GACGGCGCGG TCGGTTGCAC GTACAAGTAC CTGAACGGCG GCCCCGGTTC GCCGGCGTTC GTGTGGGTGC CGCAGCGCCA TCACGCGCAT TTCTCGCAAC CGCTGTCGGG CTGGTGGGGC CACCGCGCAC CGTTCGCGAT GCAGCCGGGC TTCGCGCCCG ATCCGGGCAT CGCGCGCTTC CTGTGCGGCA CGCAGCCGAT CGTGTCGATG TCGATGGTCG AATGCGGGCT CGACGTGTTC CTGCAGACCG ACATGCACGC GATCCGCCGC AAGTCGCTCG CGCTCACCGA TGCGTTCATC GCACTCGTCG AAGCGCGCTG CGCGGGCCTG TCGCTGAAGC TCGTCACGCC GCGCGCGCAT CACCAGCGCG GCTCGCAGGC GAGCTTCGAG CATCCGCACG GCTACGAGGT GATGCAGGCG CTGATCGCGC GCGGCGTGAT CGGCGACTAT CGCGAGCCGT ACGTGCTGCG CTTCGGCTTC ACGCCGCTCT ACACGCGCTT CGCCGACGTG TGGGATGCCG TCGAGACGCT GCGCGACATC CTGGCCACCG ATGCGTGGAA GGCACCCGAG TTCGCCGAGC GCGGCGCGGT GACCTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT