SSGCID: Target Page

BrsuB.18116.a

ErfK/YbiS/YcfS/YnhG

CENTER ID:	BrsuB.18116.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.18116.a.B1.GE38022	Full length( BrsuB.18116.a )		1	211

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_2158
BV-BRC:	fig\|470137.4.peg.2158
RefSeq:	YP_001627245.1
UniProt:	B0CKP5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGQTVPIIDV DAFGNPANRV PQANVDSSYG GWVQMYAAVE DNGYQLPAIP IQKMDTRYLR QVVPDPTGEM PGTIVIDTAN RFCYLVLDNG QALRYGVGIG REGFAWSGRA VIQYKRQWPR WTPPDEMVAR QPELVQYSAK NGGMAPGLKN PLGARALYIF KDGKDTLYRL HGNPEWWSIG KAVSSGCVRF LNQDIIDLYD RVPAKTPILV M

NT Sequence

TTGGGGCAGA CCGTTCCGAT CATCGATGTC GATGCTTTCG GCAATCCCGC CAACCGTGTA CCACAGGCCA ATGTGGATTC CTCCTACGGC GGCTGGGTGC AAATGTATGC TGCGGTAGAA GATAACGGAT ATCAGCTCCC GGCTATTCCC ATTCAGAAGA TGGACACGCG CTATCTGCGC CAGGTCGTGC CGGATCCGAC AGGCGAAATG CCCGGCACGA TCGTCATCGA TACGGCCAAT CGCTTCTGCT ATCTGGTGCT CGATAATGGG CAGGCGCTGC GTTATGGCGT CGGTATCGGT CGCGAAGGCT TTGCCTGGTC CGGCCGCGCC GTCATCCAGT ACAAGCGCCA GTGGCCGCGC TGGACCCCGC CGGATGAAAT GGTGGCCCGC CAGCCGGAAC TGGTGCAGTA CAGCGCCAAG AATGGCGGCA TGGCTCCGGG CCTCAAGAAC CCGCTCGGTG CTCGTGCGCT CTATATTTTC AAGGACGGCA AGGACACGCT CTACCGCCTG CACGGCAACC CGGAATGGTG GTCGATCGGC AAGGCTGTCT CGTCGGGCTG CGTGCGCTTC CTCAATCAGG ATATCATCGA TCTCTACGAT CGCGTGCCTG CGAAGACGCC TATCCTCGTC ATG

Details for BrsuB.18116.a.B1.GE38022

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/5/2014
EXPECTED MW:	25kDa
OBSERVED MW:	25kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML GQTVPIIDVD AFGNPANRVP QANVDSSYGG WVQMYAAVED NGYQLPAIPI QKMDTRYLRQ VVPDPTGEMP GTIVIDTANR FCYLVLDNGQ ALRYGVGIGR EGFAWSGRAV IQYKRQWPRW TPPDEMVARQ PELVQYSAKN GGMAPGLKNP LGARALYIFK DGKDTLYRLH GNPEWWSIGK AVSSGCVRFL NQDIIDLYDR VPAKTPILVM

Validated NT Sequence

atggctcacc accaccacca ccatatgttg gggcagaccg ttccgatcat cgatgtcgat gctttcggca atcccgccaa ccgtgtacca caggccaatg tggattcctc ctacggcggc tgggtgcaaa tgtatgctgc ggtagaagat aacggatatc agctcccggc tattcccatt cagaagatgg acacgcgcta tctgcgccag gtcgtgccgg atccgacagg cgaaatgccc ggcacgatcg tcatcgatac ggccaatcgc ttctgctatc tggtgctcga taatgggcag gcgctgcgtt atggcgtcgg tatcggtcgc gaaggctttg cctggtccgg ccgcgccgtc atccagtaca agcgccagtg gccgcgctgg accccgccgg atgaaatggt ggcccgccag ccggaactgg tgcagtacag cgccaagaat ggcggcatgg ctccgggcct caagaacccg ctcggtgctc gtgcgctcta tattttcaag gacggcaagg acacgctcta ccgcctgcac ggcaacccgg aatggtggtc gatcggcaag gctgtctcgt cgggctgcgt gcgcttcctc aatcaggata tcatcgatct ctacgatcgc gtgcctgcga agacgcctat cctcgtcatg taagtgagta agat

Expected Protein Sequence

MAHHHHHHMG QTVPIIDVDA FGNPANRVPQ ANVDSSYGGW VQMYAAVEDN GYQLPAIPIQ KMDTRYLRQV VPDPTGEMPG TIVIDTANRF CYLVLDNGQA LRYGVGIGRE GFAWSGRAVI QYKRQWPRWT PPDEMVARQP ELVQYSAKNG GMAPGLKNPL GARALYIFKD GKDTLYRLHG NPEWWSIGKA VSSGCVRFLN QDIIDLYDRV PAKTPILVM

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttggggc agaccgttcc gatcatcgat gtcgatgctt tcggcaatcc cgccaaccgt gtaccacagg ccaatgtgga ttcctcctac ggcggctggg tgcaaatgta tgctgcggta gaagataacg gatatcagct cccggctatt cccattcaga agatggacac gcgctatctg cgccaggtcg tgccggatcc gacaggcgaa atgcccggca cgatcgtcat cgatacggcc aatcgcttct gctatctggt gctcgataat gggcaggcgc tgcgttatgg cgtcggtatc ggtcgcgaag gctttgcctg gtccggccgc gccgtcatcc agtacaagcg ccagtggccg cgctggaccc cgccggatga aatggtggcc cgccagccgg aactggtgca gtacagcgcc aagaatggcg gcatggctcc gggcctcaag aacccgctcg gtgctcgtgc gctctatatt ttcaaggacg gcaaggacac gctctaccgc ctgcacggca acccggaatg gtggtcgatc ggcaaggctg tctcgtcggg ctgcgtgcgc ttcctcaatc aggatatcat cgatctctac gatcgcgtgc ctgcgaagac gcctatcctc gtcatgtgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t