SSGCID: Target Page

BrsuB.18114.a

L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16)

CENTER ID:	BrsuB.18114.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.18114.a.B1.GE38021	Full length( BrsuB.18114.a )		1	383

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_1136
BV-BRC:	fig\|470137.4.peg.1136
RefSeq:	YP_001622843.1
UniProt:	A9WW85

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSLADMGAEV WKIENIQGGD DTRAWSVPNY KGASTYFLCA NRGKKSLALD LKSREGLEII HELAKQADVV VENFRSGTVE RLKIDYESLK ALNPGIVYCS ISGYGQTGPE AQRPGYDFVV QAESGLMSIT GQIDGEPTRI GVAMTDIVAG MVATQSVLAA LYQRKTTGLG QYIDVSLYEC ALNTLINVGS AHLNGGHVPA RFGNAHPTVV PYQIFECSDG AFALAVGNDR QFAILCERII DLPELAADER FKTASGRALN RAALIPPMVE RFRTNTRQHW MSACLKMGVP AGQVKTVPEA FESPNVKARQ VVQKLESAHL GPISLVRPAQ GLKAQENAAY KAPPMLGEDS ASVLGDVLGL DGNKLADLIA AGVIYQYQPK DCE

NT Sequence

ATGAGCCTTG CCGACATGGG AGCCGAGGTC TGGAAGATTG AGAATATCCA GGGCGGCGAC GATACCCGCG CCTGGTCGGT GCCCAATTAC AAGGGGGCGA GCACCTATTT CCTGTGCGCC AATCGCGGCA AGAAAAGCCT GGCTCTCGAT CTGAAATCCC GTGAAGGCCT GGAAATCATC CATGAACTGG CCAAGCAGGC CGATGTGGTT GTCGAGAACT TTCGTTCAGG GACGGTCGAA CGGCTGAAGA TCGATTATGA AAGCCTGAAA GCGCTCAATC CCGGCATCGT CTATTGCTCG ATTTCCGGTT ATGGCCAGAC CGGCCCAGAG GCGCAGCGAC CGGGTTATGA TTTCGTCGTG CAGGCGGAAT CCGGCCTGAT GTCGATTACG GGGCAGATCG ATGGAGAGCC GACGCGCATC GGCGTTGCCA TGACGGATAT CGTTGCGGGC ATGGTTGCCA CGCAATCGGT CCTCGCCGCC CTTTATCAGC GCAAGACGAC GGGGCTTGGT CAATATATCG ACGTTTCACT TTATGAATGC GCGCTCAATA CATTGATCAA TGTCGGCAGC GCGCATCTGA ATGGGGGGCA TGTTCCCGCT CGCTTCGGCA ATGCCCATCC GACGGTCGTT CCCTACCAGA TTTTTGAATG TTCCGACGGT GCGTTCGCGC TGGCGGTCGG CAATGATCGC CAGTTTGCCA TTCTTTGCGA ACGGATCATC GATCTGCCCG AACTGGCCGC AGACGAGCGC TTCAAAACGG CAAGCGGTCG TGCCTTAAAC CGCGCCGCGC TGATCCCGCC AATGGTGGAA CGCTTTCGAA CCAATACCCG CCAGCACTGG ATGTCCGCAT GCCTGAAGAT GGGTGTGCCC GCAGGGCAGG TGAAAACGGT GCCGGAGGCC TTTGAAAGCC CGAACGTGAA GGCGCGGCAG GTGGTGCAGA AACTGGAAAG CGCGCATCTG GGCCCCATTT CGCTGGTGCG GCCCGCACAG GGGCTGAAAG CGCAGGAAAA CGCCGCCTAC AAGGCGCCGC CGATGCTTGG TGAAGACAGT GCCTCTGTGC TCGGAGATGT TCTCGGACTG GATGGAAACA AGCTGGCCGA TCTGATCGCG GCCGGCGTCA TTTATCAATA TCAGCCGAAG GATTGCGAA

Details for BrsuB.18114.a.B1.GE38021

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS LADMGAEVWK IENIQGGDDT RAWSVPNYKG ASTYFLCANR GKKSLALDLK SREGLEIIHE LAKQADVVVE NFRSGTVERL KIDYESLKAL NPGIVYCSIS GYGQTGPEAQ RPGYDFVVQA ESGLMSITGQ IDGEPTRIGV AMTDIVAGMV ATQSVLAALY QRKTTGLGQY IDVSLYECAL NTLINVGSAH LNGGHVPARF GNAHPTVVPY QIFECSDGAF ALAVGNDRQF AILCERIIDL PELAADERFK TASGRALNRA ALIPPMVERF RTNTRQHWMS ACLKMGVPAG QVKTVPEAFE SPNVKARQVV QKLESAHLGP ISLVRPAQGL KAQENAAYKA PPMLGEDSAS VLGDVLGLDG NKLADLIAAG VIYQYQPKDC E

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc ttgccgacat gggagccgag gtctggaaga ttgagaatat ccagggcggc gacgataccc gcgcctggtc ggtgcccaat tacaaggggg cgagcaccta tttcctgtgc gccaatcgcg gcaagaaaag cctggctctc gatctgaaat cccgtgaagg cctggaaatc atccatgaac tggccaagca ggccgatgtg gttgtcgaga actttcgttc agggacggtc gaacggctga agatcgatta tgaaagcctg aaagcgctca atcccggcat cgtctattgc tcgatttccg gttatggcca gaccggccca gaggcgcagc gaccgggtta tgatttcgtc gtgcaggcgg aatccggcct gatgtcgatt acggggcaga tcgatggaga gccgacgcgc atcggcgttg ccatgacgga tatcgttgcg ggcatggttg ccacgcaatc ggtcctcgcc gccctttatc agcgcaagac gacggggctt ggtcaatata tcgacgtttc actttatgaa tgcgcgctca atacattgat caatgtcggc agcgcgcatc tgaatggggg gcatgttccc gctcgcttcg gcaatgccca tccgacggtc gttccctacc agatttttga atgttccgac ggtgcgttcg cgctggcggt cggcaatgat cgccagtttg ccattctttg cgaacggatc atcgatctgc ccgaactggc cgcagacgag cgcttcaaaa cggcaagcgg tcgtgcctta aaccgcgccg cgctgatccc gccaatggtg gaacgctttc gaaccaatac ccgccagcac tggatgtccg catgcctgaa gatgggtgtg cccgcagggc aggtgaaaac ggtgccggag gcctttgaaa gcccgaacgt gaaggcgcgg caggtggtgc agaaactgga aagcgcgcat ctgggcccca tttcgctggt gcggcccgca caggggctga aagcgcagga aaacgccgcc tacaaggcgc cgccgatgct tggtgaagac agtgcctctg tgctcggaga tgttctcgga ctggatggaa acaagctggc cgatctgatc gcggccggcg tcatttatca atatcagccg aaggattgcg aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat