SSGCID: Target Page

BrsuB.18113.b

COG0028: Thiamine pyrophosphate-requiring enzymes

CENTER ID:	BrsuB.18113.b
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.18113.b.B1.GE38019	Full length( BrsuB.18113.b )		1	192

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_0985

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGEVMEWLRA HLPADAIITN GAGNYSAWPH RFYQYRTYRS QLAPTNGSMG YGVPAAVAAK LTAPERTVVA FAGDGCFLMN GQELATAAQY GANAIFIVVN NGMYGTIRMH QERTYPARIS GTALANPDFA ALARAYGLHG EVVEKTADFA PAFKRCEQSG KPALIEIRID PEALSPKMSL SQMREQGFAK QR

NT Sequence

ATGGGCGAGG TGATGGAGTG GCTGCGCGCG CATCTGCCCG CCGATGCCAT CATCACCAAT GGCGCGGGCA ATTATTCCGC ATGGCCGCAT CGCTTTTACC AGTATCGCAC CTATCGCAGC CAGTTGGCCC CGACCAATGG CTCCATGGGC TATGGTGTGC CGGCTGCTGT TGCGGCCAAG CTCACCGCGC CGGAGCGGAC CGTGGTGGCT TTCGCGGGAG ATGGCTGTTT CCTCATGAAC GGGCAGGAGC TTGCAACTGC CGCGCAATAT GGCGCGAATG CCATTTTCAT CGTCGTGAAC AACGGCATGT ATGGCACGAT CCGTATGCAT CAGGAGCGCA CCTATCCGGC GCGCATTTCG GGTACGGCCC TGGCCAATCC CGACTTTGCG GCGCTTGCGC GGGCCTATGG GCTGCATGGT GAAGTAGTGG AAAAGACCGC TGATTTCGCA CCCGCCTTCA AGCGGTGCGA ACAGTCCGGC AAGCCGGCGC TGATCGAGAT CAGGATCGAT CCCGAGGCAC TGTCGCCCAA GATGAGCCTC AGCCAGATGC GGGAGCAGGG TTTCGCGAAA CAGCGC

Details for BrsuB.18113.b.B1.GE38019

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/5/2014
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG EVMEWLRAHL PADAIITNGA GNYSAWPHRF YQYRTYRSQL APTNGSMGYG VPAAVAAKLT APERTVVAFA GDGCFLMNGQ ELATAAQYGA NAIFIVVNNG MYGTIRMHQE RTYPARISGT ALANPDFAAL ARAYGLHGEV VEKTADFAPA FKRCEQSGKP ALIEIRIDPE ALSPKMSLSQ MREQGFAKQR

Validated NT Sequence

atggctcacc accaccacca ccatatgggc gaggtgatgg agtggctgcg cgcgcatctg cccgccgatg ccatcatcac caatggcgcg ggcaattatt ccgcatggcc gcatcgcttt taccagtatc gcacctatcg cagccagttg gccccgacca atggctccat gggctatggt gtgccggctg ctgttgcggc caagctcacc gcgccggagc ggaccgtggt ggctttcgcg ggagatggct gtttcctcat gaacgggcag gagcttgcaa ctgccgcgca atatggcgcg aatgccattt tcatcgtcgt gaacaacggc atgtatggca cgatccgtat gcatcaggag cgcacctatc cggcgcgcat ttcgggtacg gccctggcca atcccgactt tgcggcgctt gcgcgggcct atgggctgca tggtgaagta gtggaaaaga ccgctgattt cgcacccgcc ttcaagcggt gcgaacagtc cggcaagccg gcgctgatcg agatcaggat cgatcccgag gcactgtcgc ccaagatgag cctcagccag atgcgggagc agggtttcgc gaaacagcgc taagtgagta agataggatc cggctgctaa cnancccccg aang

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggcg aggtgatgga gtggctgcgc gcgcatctgc ccgccgatgc catcatcacc aatggcgcgg gcaattattc cgcatggccg catcgctttt accagtatcg cacctatcgc agccagttgg ccccgaccaa tggctccatg ggctatggtg tgccggctgc tgttgcggcc aagctcaccg cgccggagcg gaccgtggtg gctttcgcgg gagatggctg tttcctcatg aacgggcagg agcttgcaac tgccgcgcaa tatggcgcga atgccatttt catcgtcgtg aacaacggca tgtatggcac gatccgtatg catcaggagc gcacctatcc ggcgcgcatt tcgggtacgg ccctggccaa tcccgacttt gcggcgcttg cgcgggccta tgggctgcat ggtgaagtag tggaaaagac cgctgatttc gcacccgcct tcaagcggtg cgaacagtcc ggcaagccgg cgctgatcga gatcaggatc gatcccgagg cactgtcgcc caagatgagc ctcagccaga tgcgggagca gggtttcgcg aaacagcgct gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat