SSGCID: Target Page

BrsuB.18113.a

Epi-inositol hydrolase (EC 3.7.1.-)

CENTER ID:	BrsuB.18113.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.18113.a.B1.GE38018	Full length( BrsuB.18113.a )		1	329

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_0737

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKTVRLTAAQ ALVRYLANQM TPEGDTFIAG VWAIFGHGNV AGLGEALYGA REHMPTWRGH NEQTMAHAAI AYTKQLGRRR ACAVTSSIGP GATNMVTAAA LAHVNRLPVL FVPGDVFANR GPDPVLQQIE DFNDGTMTVN DCFRPVSRYF DRITRPEQLL TALPRAFRTM TDPADCGPVT LAFCQDVQAQ AYDWPEEFFT PKVWHWRRPP PDERELETAA AAIKTALKPV IVAGGGVHYS GAHEVLRAFV ETHGIPVIET QAGKSALPWD HPLNFGPVGV TGADSANAVA AEADLVIGVG TRFQDFTTDS WALFRHPGRR LLSLKCTAL

NT Sequence

ATGAAAACCG TTCGACTGAC CGCCGCGCAG GCACTGGTGC GCTATCTGGC CAACCAGATG ACGCCGGAGG GCGACACATT CATCGCGGGC GTCTGGGCCA TTTTCGGGCA CGGCAATGTG GCGGGGCTGG GCGAGGCGCT CTACGGCGCT CGCGAGCATA TGCCCACCTG GCGCGGCCAT AACGAGCAGA CCATGGCCCA TGCGGCCATC GCCTATACCA AGCAGCTTGG CCGCAGGCGC GCCTGCGCCG TCACCTCCTC CATCGGCCCC GGCGCAACCA ATATGGTGAC AGCGGCAGCA CTTGCCCATG TGAACCGCCT GCCCGTTCTG TTCGTTCCGG GCGATGTCTT CGCCAATCGC GGTCCCGATC CAGTTCTCCA GCAGATCGAG GATTTCAACG ACGGCACCAT GACCGTCAAC GACTGTTTCC GCCCGGTAAG CCGTTATTTC GACCGTATCA CCCGGCCGGA ACAGCTTCTC ACCGCCCTGC CTCGCGCCTT TCGCACCATG ACCGACCCCG CCGATTGCGG CCCGGTGACG CTCGCCTTCT GTCAGGATGT GCAGGCGCAA GCCTATGACT GGCCGGAAGA ATTCTTCACG CCCAAGGTCT GGCACTGGCG CCGCCCGCCG CCGGACGAGC GGGAACTGGA AACCGCTGCC GCCGCCATCA AAACCGCGCT AAAGCCCGTC ATTGTGGCGG GCGGCGGCGT GCATTATTCC GGCGCGCATG AGGTGCTGCG CGCTTTTGTA GAAACACATG GCATCCCCGT TATTGAAACA CAGGCGGGCA AATCCGCACT GCCATGGGAC CATCCGCTGA ATTTCGGTCC TGTCGGCGTG ACCGGCGCTG ACAGCGCCAA TGCGGTTGCG GCAGAAGCCG ATCTGGTGAT CGGTGTCGGC ACACGCTTTC AGGACTTCAC CACCGACTCA TGGGCCCTGT TCAGGCATCC GGGGCGCAGG CTTCTTTCGC TCAAATGTAC AGCCCTA

Details for BrsuB.18113.a.B1.GE38018

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	37kDa
OBSERVED MW:	37kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMK TVRLTAAQAL VRYLANQMTP EGDTFIAGVW AIFGHGNVAG LGEALYGARE HMPTWRGHNE QTMAHAAIAY TKQLGRRRAC AVTSSIGPGA TNMVTAAALA HVNRLPVLFV PGDVFANRGP DPVLQQIEDF NDGTMTVNDC FRPVSRYFDR ITRPEQLLTA LPRAFRTMTD PADCGPVTLA FCQDVQAQAY DWPEEFFTPK VWHWRRPPPD ERELETAAAA IKTALKPVIV AGGGVHYSGA HXVLRAFVET HGIPVIETQA GKSALPWDHP LNFGPVGVTG ADSANAVAAE ADLVIGVGTR FQDFTTGSWA LFRHPGRRLL SLKCTAL

Validated NT Sequence

cnctagatat tttgtttnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgaaaac cgttcgactg accgccgcgc aggcactggt gcgctatctg gccaaccaga tgacgccgga gggcgacaca ttcatcgcgg gcgtctgggc cattttcggg cacggcaatg tggcggggct gggcgaggcg ctctacggcg ctcgcgagca tatgcccacc tggcgcggcc ataacgagca gaccatggcc catgcggcca tcgcctatac caagcagctt ggccgcaggc gcgcctgcgc cgtcacctcc tccatcggcc ccggcgcaac caatatggtg acagcggcag cacttgccca tgtgaaccgc ctgcccgttc tgttcgttcc gggcgatgtc ttcgccaatc gcggtcccga tccagttctc cagcagatcg aggatttcaa cgacggcacc atgaccgtca acgactgttt ccgcccggta agccgttatt tcgaccgtat cacccggccg gaacagcttc tcaccgccct gcctcgcgcc tttcgcacca tgaccgaccc cgccgattgc ggcccggtga cgctcgcctt ctgtcaggat gtgcaggcgc aagcctatga ctggccggaa gaattcttca cgcccaaggt ctggcactgg cgccgcccgc cgccggacga gcgggaactg gaaaccgctg ccgccgccat caaaaccgcg ctgaagcccg tcattgtggc gggcggcggc gtgcattatt ccggcgcgca tgangtgctg cgcgcttttg tagaaacaca tggcatcccc gttattgaaa cacaggcggg caaatccgca ctgccatggg accatccgct gaatttcggt cctgtcggcg tgaccggcgc tgacagcgcc aatgcggttg cggcagaagc cgatctggtg atcggtgtcg gcacacgctt tcaggacttc accaccggct catgggccct gttcaggcat cccgggcgca ggcttctttc gctcaaatgt acagccctat aagtgagtaa gatanatnnn nnnnnncnnn nnggannagn annnngnnnn nnnnnnnana nccccccnna gncnnncnac agngnnngcn nnnncnncgn ggtggnnnnn ntntanannn gnggnntgnn nnccncnnnn nnaannnagt nttctgtnnn nnnnncgccn nnntnnnnnn ntnnncnnnc cnnnnnnnnn ncgcnnna

Expected Protein Sequence

MAHHHHHHMK TVRLTAAQAL VRYLANQMTP EGDTFIAGVW AIFGHGNVAG LGEALYGARE HMPTWRGHNE QTMAHAAIAY TKQLGRRRAC AVTSSIGPGA TNMVTAAALA HVNRLPVLFV PGDVFANRGP DPVLQQIEDF NDGTMTVNDC FRPVSRYFDR ITRPEQLLTA LPRAFRTMTD PADCGPVTLA FCQDVQAQAY DWPEEFFTPK VWHWRRPPPD ERELETAAAA IKTALKPVIV AGGGVHYSGA HEVLRAFVET HGIPVIETQA GKSALPWDHP LNFGPVGVTG ADSANAVAAE ADLVIGVGTR FQDFTTDSWA LFRHPGRRLL SLKCTAL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaaa ccgttcgact gaccgccgcg caggcactgg tgcgctatct ggccaaccag atgacgccgg agggcgacac attcatcgcg ggcgtctggg ccattttcgg gcacggcaat gtggcggggc tgggcgaggc gctctacggc gctcgcgagc atatgcccac ctggcgcggc cataacgagc agaccatggc ccatgcggcc atcgcctata ccaagcagct tggccgcagg cgcgcctgcg ccgtcacctc ctccatcggc cccggcgcaa ccaatatggt gacagcggca gcacttgccc atgtgaaccg cctgcccgtt ctgttcgttc cgggcgatgt cttcgccaat cgcggtcccg atccagttct ccagcagatc gaggatttca acgacggcac catgaccgtc aacgactgtt tccgcccggt aagccgttat ttcgaccgta tcacccggcc ggaacagctt ctcaccgccc tgcctcgcgc ctttcgcacc atgaccgacc ccgccgattg cggcccggtg acgctcgcct tctgtcagga tgtgcaggcg caagcctatg actggccgga agaattcttc acgcccaagg tctggcactg gcgccgcccg ccgccggacg agcgggaact ggaaaccgct gccgccgcca tcaaaaccgc gctaaagccc gtcattgtgg cgggcggcgg cgtgcattat tccggcgcgc atgaggtgct gcgcgctttt gtagaaacac atggcatccc cgttattgaa acacaggcgg gcaaatccgc actgccatgg gaccatccgc tgaatttcgg tcctgtcggc gtgaccggcg ctgacagcgc caatgcggtt gcggcagaag ccgatctggt gatcggtgtc ggcacacgct ttcaggactt caccaccgac tcatgggccc tgttcaggca tccggggcgc aggcttcttt cgctcaaatg tacagcccta tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat