SSGCID: Target Page

BrsuB.18082.a

FIG00641944: hypothetical protein

CENTER ID:	BrsuB.18082.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.18082.a.B1.GE38016	Full length( BrsuB.18082.a )		1	268

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_0147
BV-BRC:	fig\|470137.4.peg.147
RefSeq:	YP_001621996.1
UniProt:	A9WXK8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MESQSRGRAG NISSTIVDQG SDAVRDALRK LQSEGIRYAV LDALNDQHIE TLGRAVSQMK LVTGGSGLAD GMARAWTQLR GKNVAAAEAA GAPVKGRTVI LSGSCSQMTN AQVAAYKAKA PALAMDVEKA INDAAYIDVL AEWVLAQSGD ALPPLVYATM PPEALKAVQE RFGGERASAA IEDLFGQLAK RLEAEGFTRF IVAGGETSGA VTQALAIDGF TIRPQIAPGV PWVRGIGKPL SLALKSGNFG TEAFFFEAQK IANQEGDK

NT Sequence

ATGGAATCCC AGTCGCGCGG ACGTGCAGGA AATATTTCCA GCACTATCGT CGATCAGGGC AGCGATGCAG TGCGCGACGC ACTCAGGAAG CTTCAGTCCG AAGGCATCCG CTATGCCGTC CTCGACGCCC TGAACGACCA GCATATAGAA ACGCTTGGGC GTGCCGTATC GCAAATGAAG CTGGTTACGG GCGGCTCGGG CCTTGCGGAC GGGATGGCGC GCGCATGGAC ACAACTGCGC GGCAAGAATG TTGCGGCGGC TGAAGCTGCC GGCGCGCCTG TGAAGGGACG CACGGTCATC CTCTCCGGTT CCTGCTCCCA GATGACCAAT GCGCAGGTGG CCGCATACAA GGCAAAGGCC CCCGCGCTGG CCATGGATGT CGAGAAGGCC ATAAACGACG CGGCCTATAT CGATGTTCTG GCCGAATGGG TGCTCGCACA GTCTGGCGAT GCTTTGCCGC CGCTCGTTTA TGCAACCATG CCGCCGGAAG CACTGAAAGC AGTGCAGGAG CGATTTGGTG GCGAACGTGC AAGCGCTGCC ATCGAAGATT TGTTCGGGCA GCTTGCGAAA CGTCTCGAAG CCGAAGGGTT TACGCGCTTC ATTGTTGCAG GCGGAGAAAC ATCCGGGGCT GTGACACAGG CTTTGGCAAT CGATGGGTTT ACCATCAGGC CCCAGATTGC GCCCGGCGTT CCCTGGGTAC GCGGGATCGG AAAACCATTA TCGCTTGCAC TGAAATCCGG AAATTTCGGC ACTGAGGCTT TCTTCTTTGA AGCCCAAAAG ATTGCCAATC AGGAAGGAGA TAAA

Details for BrsuB.18082.a.B1.GE38016

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	29
PERCENT COVERAGE:	34

Validated AA Sequence

MAHHHHHHXG IPVARXCRKY FQHYRRSGQR CSARRTQEAS VRRHPLCRPR RPERPAYRNA WACRIANEAG YGRLGPCRRD GARMDTTARQ ECCGG

Validated NT Sequence

atcttactca cttatttatc tccttcctga ttggcaatct tttgggcttc aaangaagaa agcctcagtg ccgaaatttc cggatttcag tgcaagcgat aatggttttc cgatcccgcg tacccaggga acgccgggcg caatctgggg cctgatggta aacccatcga ttgccaaagc ctgtgtcaca gccccggatg tttctccgcc tgcaacaatg aagcgcgtaa acccttcggc ttcgagacgt ttcgcaagct gcccgaacaa atcttcgatg gcagcgcttg cacgttcgcc accaaatcgc tcctgcactg ctttcagtgc ttccggcggc atggttgcat aaacgagcgg cgccaaagca tcgccagact gtgcgagcac ccattcggcc agaacatcga tataggccgc gtcgtttatg gccttctcga catccatggc cagcgcgggg gcctttgcct tgtatgcggc cacctgcgca ttggtcatct gggagcagga accggagagg atgaccgtgc gtcccttcac aggcgcgccg gcagcttcag ccgccgcaac attcttgccg cgcagttgtg tccatgcgcg cgccatcccg tctgcaaggc ccgagccgcc cgtaaccagc ttcatttgcg atacggcacg cccaagcgtt tctatatgct ggtcgttcag ggcgtcgagg acggcatagc ggatgccttc ggactgaagc ttcctgagtg cgtcgcgcac tgcatcgctg ccctgatcga cgatagtgct ggaaatattt cctgcacntc cgcgcgactg ggattcccnt atggtggtgg tggtggtgag ccat

Expected Protein Sequence

MAHHHHHHME SQSRGRAGNI SSTIVDQGSD AVRDALRKLQ SEGIRYAVLD ALNDQHIETL GRAVSQMKLV TGGSGLADGM ARAWTQLRGK NVAAAEAAGA PVKGRTVILS GSCSQMTNAQ VAAYKAKAPA LAMDVEKAIN DAAYIDVLAE WVLAQSGDAL PPLVYATMPP EALKAVQERF GGERASAAIE DLFGQLAKRL EAEGFTRFIV AGGETSGAVT QALAIDGFTI RPQIAPGVPW VRGIGKPLSL ALKSGNFGTE AFFFEAQKIA NQEGDK

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggaat cccagtcgcg cggacgtgca ggaaatattt ccagcactat cgtcgatcag ggcagcgatg cagtgcgcga cgcactcagg aagcttcagt ccgaaggcat ccgctatgcc gtcctcgacg ccctgaacga ccagcatata gaaacgcttg ggcgtgccgt atcgcaaatg aagctggtta cgggcggctc gggccttgcg gacgggatgg cgcgcgcatg gacacaactg cgcggcaaga atgttgcggc ggctgaagct gccggcgcgc ctgtgaaggg acgcacggtc atcctctccg gttcctgctc ccagatgacc aatgcgcagg tggccgcata caaggcaaag gcccccgcgc tggccatgga tgtcgagaag gccataaacg acgcggccta tatcgatgtt ctggccgaat gggtgctcgc acagtctggc gatgctttgc cgccgctcgt ttatgcaacc atgccgccgg aagcactgaa agcagtgcag gagcgatttg gtggcgaacg tgcaagcgct gccatcgaag atttgttcgg gcagcttgcg aaacgtctcg aagccgaagg gtttacgcgc ttcattgttg caggcggaga aacatccggg gctgtgacac aggctttggc aatcgatggg tttaccatca ggccccagat tgcgcccggc gttccctggg tacgcgggat cggaaaacca ttatcgcttg cactgaaatc cggaaatttc ggcactgagg ctttcttctt tgaagcccaa aagattgcca atcaggaagg agataaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at