BrsuB.17716.a

Urease alpha subunit (EC 3.5.1.5)

CENTER ID: BrsuB.17716.a
ORGANISM: Brucella suis ATCC 23445
ASSOCIATED DISEASE: Brucellosis
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BrsuB.17716.a.B1.GE38013 Full length( BrsuB.17716.a ) 1 558
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: PATRIC_Locus_Tag:VBIBruSui83806_1840
BV-BRC: fig|470137.4.peg.1840
RefSeq: YP_001626958.1
UniProt: B0CJP7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFGPTVGDKV RLADTDLIIE VERDLTTYGE EVKFGGGKVI RDGMGQSQLS RAEGAMDTVI TNALILDHSG IYKADIGLLD GRIALIGKAG NPDTQPGISI IIGPGTEIIA GEGKIVTAGG IDTHVHFISP QQVDEALNAG ITCMVGGGTG PAHGTLATTC TPGPWHIARL IQSFDGLPMN IGVFGKGNAS LPGALEEMVR AGACGLKLHE DWGCTPAAID NCLSVADHFD VQVAIHTDTL NEGGFVEDTL NAFKGRTIHS FHTEGAGGGH APDIIRVCQY PNVLPASTNP TRPYTVNTIA EHLDMLMVCH HLSPAIPEDI AFAESRIRKE TIAAEDILHD MGAFSIISSD SQAMGRVGEM IIRCWQTADK MKKQRGSLPD DRPGNDNYRA RRYIAKYTIN PAIAHGMAHE IGSVEVGKRA DLVLWNPAFF GVKPDMVLLG GWIATAPMGD ANGSIPTPQP MHTRPMFGSF GKARTNTSIT FVSQAAMDEG LREKIGVDKQ LVAVVNTRGG IGKHSMILNN AMPQMEVDPE TYEVRADGEL LTCEPVDVVP MAQRYFLF
NT Sequence
ATGTTCGGCC CCACCGTTGG CGACAAGGTG CGCCTTGCCG ATACGGATCT CATCATCGAA GTCGAGCGCG ACCTCACCAC CTATGGCGAG GAAGTGAAGT TCGGCGGCGG CAAGGTTATC CGCGACGGTA TGGGCCAGAG CCAGCTTTCC CGCGCCGAAG GCGCGATGGA CACCGTCATC ACCAATGCGC TGATCCTCGA CCATAGCGGC ATCTACAAGG CGGATATCGG GCTTCTCGAC GGGCGCATCG CCCTCATCGG CAAGGCAGGC AATCCCGATA CGCAGCCCGG CATCTCGATC ATCATCGGCC CCGGCACGGA GATCATCGCT GGCGAAGGCA AGATCGTCAC CGCGGGCGGC ATCGATACCC ATGTCCATTT CATCTCGCCG CAACAGGTGG ATGAGGCGCT GAATGCGGGC ATCACCTGCA TGGTTGGCGG CGGCACCGGC CCCGCGCATG GCACGCTTGC CACCACCTGC ACGCCTGGCC CCTGGCACAT CGCCCGGCTG ATCCAGTCCT TCGATGGCCT GCCGATGAAT ATCGGTGTGT TCGGCAAGGG CAATGCCTCG CTGCCCGGCG CATTGGAAGA AATGGTTCGC GCCGGCGCCT GCGGCCTGAA ACTGCATGAG GACTGGGGTT GCACACCGGC GGCTATCGAC AATTGCCTTT CGGTTGCCGA TCATTTCGAC GTTCAGGTGG CGATCCACAC CGATACGCTC AATGAAGGTG GCTTCGTTGA AGATACGCTC AATGCCTTCA AGGGACGCAC GATCCACTCT TTCCATACGG AAGGTGCGGG CGGTGGGCAC GCGCCCGATA TCATCCGTGT TTGCCAATAT CCGAACGTGC TGCCTGCTTC GACCAACCCG ACCCGGCCCT ACACGGTCAA CACGATTGCG GAACATCTCG ACATGCTGAT GGTCTGCCAT CACCTGTCGC CTGCAATCCC TGAGGATATC GCCTTTGCCG AAAGCCGCAT CCGCAAGGAA ACGATTGCGG CGGAAGATAT TCTGCACGAC ATGGGCGCCT TCTCGATCAT TTCCTCCGAC AGCCAGGCGA TGGGCCGTGT GGGTGAAATG ATCATCCGCT GCTGGCAGAC GGCAGACAAG ATGAAGAAAC AGCGCGGCAG CCTGCCTGAC GATAGGCCGG GCAACGACAA TTACCGCGCC CGCCGTTATA TTGCCAAATA TACGATCAAT CCTGCTATCG CGCATGGCAT GGCGCATGAG ATCGGCTCCG TGGAAGTGGG CAAGCGTGCG GATCTGGTGC TGTGGAACCC GGCCTTCTTC GGTGTCAAAC CGGATATGGT GCTGCTTGGC GGCTGGATCG CCACCGCCCC CATGGGCGAT GCAAACGGTT CCATCCCCAC TCCGCAGCCT ATGCATACCC GCCCCATGTT CGGCTCGTTC GGCAAGGCGC GCACCAACAC GTCCATCACG TTTGTTTCGC AAGCAGCGAT GGATGAGGGC CTGCGTGAGA AGATCGGCGT GGACAAGCAG CTGGTGGCGG TCGTCAATAC GCGCGGCGGC ATCGGCAAGC ATTCCATGAT CCTCAACAAT GCCATGCCGC AGATGGAAGT GGACCCGGAA ACCTATGAAG TGCGTGCGGA CGGCGAACTT CTCACCTGTG AGCCGGTAGA CGTGGTACCA ATGGCGCAAC GCTACTTCCT GTTT
Details for BrsuB.17716.a.B1.GE38013
HARVESTED ON: 5/12/2014
SEQUENCED ON: 5/4/2014
EXPECTED MW: 61kDa
OBSERVED MW: 65kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 72
Validated AA Sequence
MAHHHHHHMF GPTVGDKVRL ADTDLIIEVE RDLTTYGEEV KFGGGKVIRD GMGQSQLSRA EGAMDTVITN ALILDHSGIY KADIGLLDGR IALIGKAGNP DTQPGISIII GPGTEIIAGE GKIVTAGGID THVHFISPQQ VDEALNAGIT CMVGGGTGPA HGTLATTCTP GPWHIARLIQ SFDGLPMNIG VFGKGNASLP GALEEMVRAG ACGLKLHEDW GCTPAAIDNC LSVADHFDVQ VAIHTDTLNE GGFVEDTLNA FKGRTIHSFH TEGAGGGHAP DIIRVCQYPN VLPASTNPTR PYTVNTIAEH LDMLMVCHHL SPAIPEDXAF AESRIRKETI XXXDXXARHG RLLDHFLRXX XWXXXGEXSS LLQXXXXMXN XPXXXXRXXX XXXRPXXXXX XXXXXLXXXX
Validated NT Sequence
ctagaatatt ttgtttaact ttaagaagga gatataccat ggctcaccac caccaccacc atatgttcgg ccccaccgtt ggcgacaagg tgcgccttgc cgatacggat ctcatcatcg aagtcgagcg cgacctcacc acctatggcg aggaagtgaa gttcggcggc ggcaaggtta tccgcgacgg tatgggccag agccagcttt cccgcgccga aggcgcgatg gacaccgtca tcaccaatgc gctgatcctc gaccatagcg gcatctataa ggcggatatc gggcttctcg acgggcgcat cgccctcatc ggcaaggcag gcaatcccga tacgcagccc ggcatctcga tcatcatcgg ccccggcacg gagatcatcg ctggcgaagg caagatcgtc accgcgggcg gcatcgatac ccatgtccat ttcatctcgc cgcaacaggt ggatgaggcg ctgaatgcgg gcatcacctg catggttggc ggcggcaccg gccccgcgca tggcacgctt gccaccacct gcacgcctgg cccctggcac atcgcccggc tgatccagtc cttcgatggc ctgccgatga atatcggtgt gttcggcaag ggcaatgcct cgctgcccgg cgcattggaa gaaatggttc gcgccggcgc ctgcggcctg aaactgcatg aggactgggg ttgcacaccg gcggctatcg acaattgcct ttcggttgcc gatcatttcg acgttcaggt ggcgatccac accgatacgc tcaatgaagg tggcttcgtt gaagatacgc tcaatgcctt caagggacgc acgatccact ctttccatac ggaaggtgcg ggcggtgggc acgcgcccga tatcatccgt gtttgccaat atccgaacgt gctgcctgct tcgaccaacc cgacccggcc ctacacggtc aacacgattg cggaacatct cgacatgctg atggtctgcc atcacctgtc gcctgcaatc cctgaggatn ncgcctttgc cgaaagccgc atccgcaagg aaacgattgn nncnnaagat antnctgcac gacatgggcg ccttctcgat catttcctcc gacnnnngnn atggnnnnng nggggtgaan gatcatcnct gctgcagang nnnnacaana tganaaacnn nccgncagnc nnnnnacgaa nngnnnnnaa nnnnnnccgg cccnnntnnn tttntntncn nnncantcnc tgncttatnn nncnccantg
Expected Protein Sequence
MAHHHHHHMF GPTVGDKVRL ADTDLIIEVE RDLTTYGEEV KFGGGKVIRD GMGQSQLSRA EGAMDTVITN ALILDHSGIY KADIGLLDGR IALIGKAGNP DTQPGISIII GPGTEIIAGE GKIVTAGGID THVHFISPQQ VDEALNAGIT CMVGGGTGPA HGTLATTCTP GPWHIARLIQ SFDGLPMNIG VFGKGNASLP GALEEMVRAG ACGLKLHEDW GCTPAAIDNC LSVADHFDVQ VAIHTDTLNE GGFVEDTLNA FKGRTIHSFH TEGAGGGHAP DIIRVCQYPN VLPASTNPTR PYTVNTIAEH LDMLMVCHHL SPAIPEDIAF AESRIRKETI AAEDILHDMG AFSIISSDSQ AMGRVGEMII RCWQTADKMK KQRGSLPDDR PGNDNYRARR YIAKYTINPA IAHGMAHEIG SVEVGKRADL VLWNPAFFGV KPDMVLLGGW IATAPMGDAN GSIPTPQPMH TRPMFGSFGK ARTNTSITFV SQAAMDEGLR EKIGVDKQLV AVVNTRGGIG KHSMILNNAM PQMEVDPETY EVRADGELLT CEPVDVVPMA QRYFLF
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcg gccccaccgt tggcgacaag gtgcgccttg ccgatacgga tctcatcatc gaagtcgagc gcgacctcac cacctatggc gaggaagtga agttcggcgg cggcaaggtt atccgcgacg gtatgggcca gagccagctt tcccgcgccg aaggcgcgat ggacaccgtc atcaccaatg cgctgatcct cgaccatagc ggcatctaca aggcggatat cgggcttctc gacgggcgca tcgccctcat cggcaaggca ggcaatcccg atacgcagcc cggcatctcg atcatcatcg gccccggcac ggagatcatc gctggcgaag gcaagatcgt caccgcgggc ggcatcgata cccatgtcca tttcatctcg ccgcaacagg tggatgaggc gctgaatgcg ggcatcacct gcatggttgg cggcggcacc ggccccgcgc atggcacgct tgccaccacc tgcacgcctg gcccctggca catcgcccgg ctgatccagt ccttcgatgg cctgccgatg aatatcggtg tgttcggcaa gggcaatgcc tcgctgcccg gcgcattgga agaaatggtt cgcgccggcg cctgcggcct gaaactgcat gaggactggg gttgcacacc ggcggctatc gacaattgcc tttcggttgc cgatcatttc gacgttcagg tggcgatcca caccgatacg ctcaatgaag gtggcttcgt tgaagatacg ctcaatgcct tcaagggacg cacgatccac tctttccata cggaaggtgc gggcggtggg cacgcgcccg atatcatccg tgtttgccaa tatccgaacg tgctgcctgc ttcgaccaac ccgacccggc cctacacggt caacacgatt gcggaacatc tcgacatgct gatggtctgc catcacctgt cgcctgcaat ccctgaggat atcgcctttg ccgaaagccg catccgcaag gaaacgattg cggcggaaga tattctgcac gacatgggcg ccttctcgat catttcctcc gacagccagg cgatgggccg tgtgggtgaa atgatcatcc gctgctggca gacggcagac aagatgaaga aacagcgcgg cagcctgcct gacgataggc cgggcaacga caattaccgc gcccgccgtt atattgccaa atatacgatc aatcctgcta tcgcgcatgg catggcgcat gagatcggct ccgtggaagt gggcaagcgt gcggatctgg tgctgtggaa cccggccttc ttcggtgtca aaccggatat ggtgctgctt ggcggctgga tcgccaccgc ccccatgggc gatgcaaacg gttccatccc cactccgcag cctatgcata cccgccccat gttcggctcg ttcggcaagg cgcgcaccaa cacgtccatc acgtttgttt cgcaagcagc gatggatgag ggcctgcgtg agaagatcgg cgtggacaag cagctggtgg cggtcgtcaa tacgcgcggc ggcatcggca agcattccat gatcctcaac aatgccatgc cgcagatgga agtggacccg gaaacctatg aagtgcgtgc ggacggcgaa cttctcacct gtgagccggt agacgtggta ccaatggcgc aacgctactt cctgttttga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at