SSGCID: Target Page

BrsuB.17659.a

Phosphopantetheinyl transferase component of siderophore synthetase (EC 2.7.8.-)

CENTER ID:	BrsuB.17659.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.17659.a.B1.GE38012	Full length( BrsuB.17659.a )		1	249

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_0011
BV-BRC:	fig\|470137.4.peg.11
RefSeq:	YP_001621866.1
UniProt:	A9WX41

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSWMAEQPLA HETAWLAPGA HGVSVITPWA DLAADCCFVM TRYSASKPLI DPSIPAPARA APRRKAEFVA GQACAREALR LAGGPAVMPG RGDDHAPIWP PGMVGSISHS KGCAIAVAAR QSRYLGLGID IERLQTESSA RKIAPQILGE SELRQLPEAG RAFLVSLAFS AKESLFKALY PAVQQFQSFH AAAFSFDGDR QQACLELAVD WSAQWRRGQR FPIRFFCRDG FILTCTAIAA ISCRSEMSG

NT Sequence

TTGTCGTGGA TGGCGGAGCA ACCCTTGGCG CATGAGACCG CGTGGCTTGC GCCAGGCGCC CACGGCGTTT CGGTCATAAC GCCGTGGGCC GATCTGGCCG CAGACTGCTG TTTCGTCATG ACCCGCTATT CGGCCAGCAA ACCCTTGATA GACCCTTCTA TCCCCGCTCC TGCGCGCGCC GCCCCGCGCC GAAAGGCGGA ATTTGTCGCC GGGCAAGCCT GCGCGCGCGA AGCCCTGCGC CTTGCAGGCG GGCCTGCCGT AATGCCGGGG CGTGGGGATG ACCATGCGCC CATCTGGCCA CCGGGCATGG TCGGTTCCAT CTCCCATAGC AAGGGATGCG CAATTGCCGT CGCCGCCCGC CAGAGCCGCT ATCTGGGCCT CGGCATTGAT ATCGAACGCC TGCAAACGGA AAGTTCGGCC CGAAAAATCG CCCCGCAGAT ACTGGGTGAA AGCGAGCTGC GCCAATTGCC CGAAGCCGGC CGGGCCTTTC TGGTGAGCCT TGCTTTTTCT GCAAAAGAAA GCCTGTTCAA GGCGCTTTAT CCGGCAGTGC AGCAATTCCA GAGTTTCCAT GCCGCAGCCT TTTCCTTTGA CGGCGACAGG CAGCAAGCCT GCCTTGAGCT TGCGGTGGAC TGGTCGGCGC AATGGCGCCG CGGGCAGCGG TTCCCGATCC GCTTTTTCTG CCGCGACGGT TTCATTCTCA CCTGCACGGC CATTGCGGCC ATCAGTTGCC GTTCAGAAAT GTCAGGA

Details for BrsuB.17659.a.B1.GE38012

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHML SWMAEQPLAH ETAWLAPGAH GVSVITPWAD LAADCCFVMA RYSASKPLID PSIPAPARAA PRRKAEFVAG QACAREALRL AGGPAVMPGR GDDHAPIWPP GMVGSISHSK GCAIAVAARQ SRYLGLGIDI ERLQTESSAR KIAPQILGES ELRQLPEAGR AFLVSLAFSA KESLFKALYP AVQQFQSFHA AAFSFDGDRQ QACLELAVDW SAQWRRGQRF PIRFFCRDGF ILTCTAIAAI SCRSEMSG

Validated NT Sequence

ctnnnnattt tgtttaactt taagaaggag atataccatg gctcaccacc accaccacca tatgttgtcg tggatggcgg agcaaccctt ggcgcatgag accgcgtggc ttgcgccagg cgcccacggc gtttcggtca taacgccgtg ggccgatctg gccgcagact gctgtttcgt catggcccgc tattcggcca gcaaaccctt gatagaccct tctatccccg ctcctgcgcg cgccgccccg cgccgaaagg cggaatttgt cgccgggcaa gcctgcgcgc gcgaagccct gcgccttgca ggcgggcctg ccgtaatgcc ggggcgtggg gatgaccatg cgcccatctg gccaccgggc atggtcggtt ccatctccca tagcaaggga tgcgcaattg ccgtcgccgc ccgccagagc cgctatctgg gcctcggcat tgatatcgaa cgcctgcaaa cggaaagttc ggcccgaaaa atcgccccgc agatactggg tgaaagcgag ctgcgccaat tgcccgaagc cggccgggcc tttctggtga gccttgcttt ttctgcaaaa gaaagcctgt tcaaggcgct ttatccggca gtgcagcaat tccagagttt ccatgccgca gccttttcct ttgacggcga caggcagcaa gcctgccttg agcttgcggt ggactggtcg gcgcaatggc gccgcgggca gcggttcccg atccgctttt tctgccgcga cggtttcatt ctcacctgca cggccattgc ggccatcagt tgccgttcag aaatgtcagg ataagtgagt aagataggat ccggctgcta anaccccccc aannnnaann cnnnnnnatn cngccgncnn aaaagcttcc aaccnntcnt cctcganatg caatcaagtg agaanaaaga tcnnt

Expected Protein Sequence

MAHHHHHHMS WMAEQPLAHE TAWLAPGAHG VSVITPWADL AADCCFVMTR YSASKPLIDP SIPAPARAAP RRKAEFVAGQ ACAREALRLA GGPAVMPGRG DDHAPIWPPG MVGSISHSKG CAIAVAARQS RYLGLGIDIE RLQTESSARK IAPQILGESE LRQLPEAGRA FLVSLAFSAK ESLFKALYPA VQQFQSFHAA AFSFDGDRQQ ACLELAVDWS AQWRRGQRFP IRFFCRDGFI LTCTAIAAIS CRSEMSG

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catttgtcgt ggatggcgga gcaacccttg gcgcatgaga ccgcgtggct tgcgccaggc gcccacggcg tttcggtcat aacgccgtgg gccgatctgg ccgcagactg ctgtttcgtc atgacccgct attcggccag caaacccttg atagaccctt ctatccccgc tcctgcgcgc gccgccccgc gccgaaaggc ggaatttgtc gccgggcaag cctgcgcgcg cgaagccctg cgccttgcag gcgggcctgc cgtaatgccg gggcgtgggg atgaccatgc gcccatctgg ccaccgggca tggtcggttc catctcccat agcaagggat gcgcaattgc cgtcgccgcc cgccagagcc gctatctggg cctcggcatt gatatcgaac gcctgcaaac ggaaagttcg gcccgaaaaa tcgccccgca gatactgggt gaaagcgagc tgcgccaatt gcccgaagcc ggccgggcct ttctggtgag ccttgctttt tctgcaaaag aaagcctgtt caaggcgctt tatccggcag tgcagcaatt ccagagtttc catgccgcag ccttttcctt tgacggcgac aggcagcaag cctgccttga gcttgcggtg gactggtcgg cgcaatggcg ccgcgggcag cggttcccga tccgcttttt ctgccgcgac ggtttcattc tcacctgcac ggccattgcg gccatcagtt gccgttcaga aatgtcagga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat