SSGCID: Target Page

BrsuB.17498.a

FIG00450955: hypothetical protein

CENTER ID:	BrsuB.17498.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.17498.a.B1.GE38008	Full length( BrsuB.17498.a )		1	269

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_2166
BV-BRC:	fig\|470137.4.peg.2166
RefSeq:	YP_001627255.1
UniProt:	B0CKQ5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MEDGSMTRQI SAAETRIIRF LKQGGCELQD SVRETHALLV GEQGTIAASR LEIERMRKAG TIQCDGQRIA LSAMGRKRPK EQKKPTLTAA SVRETYEIEP GEKVEINPAE SPLAMLYRRR NADGRAFISE DEFRAGERQR ADFTRGSLMP SVTSRWDVQA GSGGHYGAGG MAELTDIALA SRIRVERALE AVGPELSGIL IDVCCFLKGL ETVERERQWP VRSAKVLLKA GLAMLHRHYN PPREGERRRG AVLHWGAEDY RPEIRPRSV

NT Sequence

GTGGAGGATG GCAGCATGAC GAGGCAGATT TCGGCAGCGG AAACGCGCAT TATCCGTTTT TTGAAGCAGG GCGGTTGCGA GCTTCAGGAT TCGGTTCGCG AAACGCACGC TTTGCTGGTT GGCGAACAGG GGACTATTGC GGCTTCGCGC CTTGAAATCG AACGGATGCG CAAGGCTGGA ACCATACAAT GTGACGGGCA GCGCATCGCG CTTTCTGCAA TGGGACGCAA GCGTCCTAAA GAGCAGAAAA AGCCCACGCT AACCGCCGCC TCTGTCCGTG AGACATACGA GATAGAGCCG GGCGAAAAGG TGGAGATCAA TCCGGCGGAA TCCCCACTTG CCATGCTTTA TCGGCGCCGG AACGCCGATG GCCGCGCCTT TATTTCGGAG GATGAATTCC GTGCGGGTGA GCGCCAGCGC GCTGATTTCA CGCGGGGGTC GCTCATGCCT TCCGTTACGT CGCGCTGGGA TGTCCAGGCC GGGTCGGGCG GGCATTATGG GGCAGGCGGC ATGGCTGAAC TGACCGATAT CGCCCTTGCA AGCCGGATAA GGGTGGAGCG TGCGCTGGAA GCTGTCGGGC CGGAACTGAG CGGCATTTTG ATAGACGTTT GCTGCTTTTT GAAAGGACTG GAAACAGTCG AGCGTGAGCG TCAATGGCCG GTTCGTTCTG CCAAGGTGCT GCTGAAAGCG GGGCTTGCCA TGCTGCATCG CCATTATAAT CCTCCGCGCG AAGGCGAAAG GCGAAGGGGG GCAGTCCTGC ATTGGGGGGC GGAGGATTAT CGCCCTGAGA TTCGACCGCG GAGTGTG

Details for BrsuB.17498.a.B1.GE38008

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	31kDa
OBSERVED MW:	33kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV EDGSMTRQIS AAETRIIRFL KQGGCELQDS VRETHALLVG EQGTIAASRL EIERMRKAGT IQCDGQRIAL SAMGRKRPKE QKKPTLTAAS VRETYEIEPG EKVEINPAES PLAMLYRRRN ADGRAFISED EFRAGERLRA DFTRGSLMPS VTSRWDVQAG SGGHYGAGGM AELTDIALAS RIRVERALEA VGPELSGILI DVCCFLKGLE TVERERQWPV RSAKVLLKAG LAMLHRHYNP PREGERRRGA VLHWGAEDYR PEIRPRSV

Validated NT Sequence

atcttactca cttacacact ccgcggtcga atctcagggc gataatcctc cgccccccaa tgcaggactg ccccccttcg cctttcgcct tcgcgcggag gattataatg gcgatgcagc atggcaagcc ccgctttcag cagcaccttg gcagaacgaa ccggccattg acgctcacgc tcgactgttt ccagtccttt caaaaagcag caaacgtcta tcaaaatgcc gctcagttcc ggcccgacag cttccagcgc acgctccacc cttatccggc ttgcaagggc gatatcggtc agttcagcca tgccgcctgc cccataatgc ccgcccgacc cggcctggac atcccagcgc gacgtaacgg aaggcatgag cgacccccgc gtgaaatcag cgcgcaggcg ctcgcccgca cggaattcat cctccgaaat aaaggcgcgg ccatcggcgt tccggcgccg ataaagcatg gcaagtgggg attccgccgg attgatctcc accttttcgc ccggctctat ctcgtatgtc tcacggacag aggcggcggt tagcgtgggc tttttctgct ctttaggacg cttgcgtccc attgcagaaa gcgcgatgcg ctgcccgtca cattgtatgg ttccagcctt gcgcatccgt tcgatttcaa ggcgcgaagc cgcaatagtc ccctgttcgc caaccagcaa agcgtgcgtt tcgcgaaccg aatcctgaag ctcgcaaccg ccctgcttca aaaaacggat aatgcgcgtt tccgctgccg aaatctgcct cgtcatgctg ccatcctcca ccatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHME DGSMTRQISA AETRIIRFLK QGGCELQDSV RETHALLVGE QGTIAASRLE IERMRKAGTI QCDGQRIALS AMGRKRPKEQ KKPTLTAASV RETYEIEPGE KVEINPAESP LAMLYRRRNA DGRAFISEDE FRAGERQRAD FTRGSLMPSV TSRWDVQAGS GGHYGAGGMA ELTDIALASR IRVERALEAV GPELSGILID VCCFLKGLET VERERQWPVR SAKVLLKAGL AMLHRHYNPP REGERRRGAV LHWGAEDYRP EIRPRSV

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtggagg atggcagcat gacgaggcag atttcggcag cggaaacgcg cattatccgt tttttgaagc agggcggttg cgagcttcag gattcggttc gcgaaacgca cgctttgctg gttggcgaac aggggactat tgcggcttcg cgccttgaaa tcgaacggat gcgcaaggct ggaaccatac aatgtgacgg gcagcgcatc gcgctttctg caatgggacg caagcgtcct aaagagcaga aaaagcccac gctaaccgcc gcctctgtcc gtgagacata cgagatagag ccgggcgaaa aggtggagat caatccggcg gaatccccac ttgccatgct ttatcggcgc cggaacgccg atggccgcgc ctttatttcg gaggatgaat tccgtgcggg tgagcgccag cgcgctgatt tcacgcgggg gtcgctcatg ccttccgtta cgtcgcgctg ggatgtccag gccgggtcgg gcgggcatta tggggcaggc ggcatggctg aactgaccga tatcgccctt gcaagccgga taagggtgga gcgtgcgctg gaagctgtcg ggccggaact gagcggcatt ttgatagacg tttgctgctt tttgaaagga ctggaaacag tcgagcgtga gcgtcaatgg ccggttcgtt ctgccaaggt gctgctgaaa gcggggcttg ccatgctgca tcgccattat aatcctccgc gcgaaggcga aaggcgaagg ggggcagtcc tgcattgggg ggcggaggat tatcgccctg agattcgacc gcggagtgtg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat