SSGCID: Target Page

BrsuB.17468.a

Acetyltransferase, GNAT family

CENTER ID:	BrsuB.17468.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.17468.a.B1.GE38007	Full length( BrsuB.17468.a )		1	169

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_3195
BV-BRC:	fig\|470137.4.peg.3195
RefSeq:	YP_001628209.1
UniProt:	B0CHY8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKGGRMHIRK ERPQDAAEIR QVTEAAFRPV AYSNQKEGEI VDALRAAKAL TLSLVAEEDG QVLGHIAFSP VLIGGAEKGW YGLGPVSVLP ARQGEGIGGK LIREGLAQLR GAGARGCVLL GDLGYYGRFG FKADARQKLP GVPPEYFQCL AFGPDMPQGD VAYHAAFDA

NT Sequence

GTGAAGGGAG GCAGAATGCA TATCCGCAAG GAAAGGCCAC AAGACGCCGC CGAAATCCGT CAGGTGACAG AGGCGGCTTT TCGCCCTGTT GCTTATAGCA ACCAGAAGGA AGGGGAGATT GTCGATGCGC TTCGCGCGGC AAAGGCGCTT ACCCTGTCGC TGGTTGCGGA GGAAGACGGG CAGGTTCTGG GCCATATCGC CTTTTCGCCG GTCCTGATCG GCGGTGCGGA AAAGGGTTGG TACGGGCTTG GTCCCGTTTC AGTGCTGCCC GCCCGGCAAG GTGAGGGGAT TGGCGGCAAG CTGATCCGCG AGGGCCTTGC GCAATTGCGC GGTGCCGGGG CAAGGGGCTG TGTTCTTCTT GGCGACCTCG GCTATTACGG ACGCTTTGGT TTCAAGGCCG ATGCGCGGCA GAAACTGCCG GGGGTGCCGC CCGAATATTT CCAGTGTCTG GCCTTCGGTC CGGATATGCC GCAAGGCGAT GTTGCCTATC ATGCGGCATT CGATGCA

Details for BrsuB.17468.a.B1.GE38007

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	19kDa
OBSERVED MW:	18kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with sequence variation
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMV KGGRMHIRKE RPQDAAEIRQ VTEAAFRPVA YSNQKEGEIV DALRAAKALT LSLVAEEDGQ VLGHIAFSPV LIGGAEKGWY GLGPVSVLPA RQGEGIGGKL IREGLAQLRG AGARGCVLLG DLGYYGRFGF KADARQKLPG VPPEYFQCLA FGPDMPQGDV AYHAAFDA

Validated NT Sequence

antccnctag antattttgt ttaactttaa gaaggagata taccatggct caccaccacc accaccatat ggtgaaggga ggcagaatgc atatccgcaa ggaaaggcca caagacgccg ccgaaatccg tcaggtgaca gaggcggctt ttcgccctgt tgcttatagc aaccagaagg aaggggagat tgtcgatgcg cttcgcgcgg caaaggcgct taccctgtcg ctggttgcgg aggaagacgg gcaggttctg ggccatatcg ccttttcgcc ggtcctgatc ggcggtgcgg aaaagggttg gtacgggctt ggtcccgttt cagtgctgcc cgcccggcaa ggtgagggga ttggcggcaa gctgatccgc gagggccttg cgcaattgcg cggtgccggg gcaaggggct gtgttcttct tggcgacctc ggttattacg gacgctttgg tttcaaggcc gatgcgcggc agaaactgcc gggggtgccg cccgaatatt tccagtgtct ggccttcggt ccggatatgc cgcaaggcga tgttgcctat catgcggcat tcgatgcata agtgagtaag ataggatccg gctgctaaca accccggang angnnnnngn nctgatctgt ancctgacga gccggaactg accgggattt tnatagacgt ttgctgcttt ttgaaaagac tgaactacaa aacgagcgtg agcgtcattg accggttctt tctgccaagg tgctgctgaa agcggggctt gccatgccgc atcgccatta taatcctccg cgcgaaggca aacggcggan ggggggcagt cctgcaatgg gggggcggaa gattctcncc ctgatgttcc cacccgnaaa tttcagttga gtaagatatg accgnnnntg accgccccaa tgcggtgcgg cgaagcctat ctggtgatcg gtgtcggcnc cncttttcgg acttcnacac cgcctnaagg gccctgttca tgctcccggg cgcaggcttc tttccctcaa atgncagcct ntaatgaata aaaaggacc

Expected Protein Sequence

MAHHHHHHMK GGRMHIRKER PQDAAEIRQV TEAAFRPVAY SNQKEGEIVD ALRAAKALTL SLVAEEDGQV LGHIAFSPVL IGGAEKGWYG LGPVSVLPAR QGEGIGGKLI REGLAQLRGA GARGCVLLGD LGYYGRFGFK ADARQKLPGV PPEYFQCLAF GPDMPQGDVA YHAAFDA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgaagg gaggcagaat gcatatccgc aaggaaaggc cacaagacgc cgccgaaatc cgtcaggtga cagaggcggc ttttcgccct gttgcttata gcaaccagaa ggaaggggag attgtcgatg cgcttcgcgc ggcaaaggcg cttaccctgt cgctggttgc ggaggaagac gggcaggttc tgggccatat cgccttttcg ccggtcctga tcggcggtgc ggaaaagggt tggtacgggc ttggtcccgt ttcagtgctg cccgcccggc aaggtgaggg gattggcggc aagctgatcc gcgagggcct tgcgcaattg cgcggtgccg gggcaagggg ctgtgttctt cttggcgacc tcggctatta cggacgcttt ggtttcaagg ccgatgcgcg gcagaaactg ccgggggtgc cgcccgaata tttccagtgt ctggccttcg gtccggatat gccgcaaggc gatgttgcct atcatgcggc attcgatgca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat