SSGCID: Target Page

BrsuB.17387.a

Citrate lyase beta chain (EC 4.1.3.6)

CENTER ID:	BrsuB.17387.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.17387.a.B1.GE38004	Full length( BrsuB.17387.a )		1	112

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_0917

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQIVLAARTG GIAVLDGVYN DFRDSAGFEA ECAQGAAMGF DGKTLIHPGQ IEAANRAFSP TEEEVAHARA VREIFARPEN AGKGVVSLDG QMVERLHLEM AERILAKAGI NL

NT Sequence

ATGCAGATTG TGCTTGCGGC GCGCACTGGC GGCATTGCGG TGCTGGATGG CGTTTATAAT GATTTTCGTG ATAGCGCTGG CTTCGAGGCC GAATGTGCGC AGGGCGCGGC CATGGGTTTT GACGGCAAGA CGCTGATCCA TCCGGGCCAG ATCGAAGCGG CCAATCGCGC CTTCTCACCG ACGGAGGAGG AAGTGGCCCA TGCGCGCGCA GTGCGCGAGA TTTTTGCCCG GCCGGAAAAT GCCGGCAAGG GCGTCGTTTC GCTGGACGGG CAAATGGTCG AGCGCCTGCA TCTGGAAATG GCCGAACGGA TTCTCGCGAA GGCTGGTATA AATCTG

Details for BrsuB.17387.a.B1.GE38004

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	13kDa
OBSERVED MW:	13kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	99
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMQ IVLAARTGGI AVLDGVYNDF RDSAGFEAEC AQGAAMGFDG KTLIHPGQIE AANRAFSPTE EEVAHARAVR EIFAWPENAG KGVVSLDGQM VERLHLEMAE RILAKAGINL

Validated NT Sequence

cnctagaata ttttgtttaa ctttaagaag gagatatacc atggctcacc accaccacca ccatatgcag attgtgcttg cggcgcgcac tggcggcatt gcggtgctgg atggcgttta taatgatttt cgtgatagcg ctggcttcga ggccgaatgt gcgcagggcg cggccatggg ttttgacggc aagacgctga tccatccggg ccagatcgaa gcggccaatc gcgccttctc accgacggag gaggaagtgg cccatgcgcg cgcagtgcgc gagatttttg cctggccgga aaatgccggc aagggcgtcg tttcgctgga cgggcaaatg gtcgagcgcc tgcatctgga aatggccgaa cggattctcg cgaaggctgg tataaatctg taagtgagta agataggatc cggctgctaa cagnncccna aagaaaatga agttctcttn tcttgttgtt tctcatggcg tggcgcttct cgtccacgtc cattcgcata tcgacatccc ccatgcgtgg gttgccatca tcgctgagat ccaggcctat cgctccacgg cagatctgga cgcgctccga ctcaagagca ttgtcctgat cagcgatcgc cagggctaca acttctcgct gcaccgcgac gctcgctatc gtcacgtgat agaaanacag ggacactgct ctgacccccc acccgaggga acacggcgat cccactgatg cacagaccta tcgcntctcc ggcaatcttc tggattccat gcgcgaagaa aggcatntcn tcgcaggctc catgatgnan agcnncgcgt gctactgctt ctncgagctc cttcangnct acgatcggaa gtgagatnag cnncatggnc ccggangacg tganctcact gctcatctcg cgcnntatga tgtcgacggt gantgcntac gctattcatg annnggctgg cagnnatgcg cctactnttg attganatgc ncgagtgnnc nangcnctca ncgaaancca tnaattacng cccggannaa ctgatcctaa nnncnatcnn cccacccnng tntcnatang nntanaaga

Expected Protein Sequence

MAHHHHHHMQ IVLAARTGGI AVLDGVYNDF RDSAGFEAEC AQGAAMGFDG KTLIHPGQIE AANRAFSPTE EEVAHARAVR EIFARPENAG KGVVSLDGQM VERLHLEMAE RILAKAGINL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaga ttgtgcttgc ggcgcgcact ggcggcattg cggtgctgga tggcgtttat aatgattttc gtgatagcgc tggcttcgag gccgaatgtg cgcagggcgc ggccatgggt tttgacggca agacgctgat ccatccgggc cagatcgaag cggccaatcg cgccttctca ccgacggagg aggaagtggc ccatgcgcgc gcagtgcgcg agatttttgc ccggccggaa aatgccggca agggcgtcgt ttcgctggac gggcaaatgg tcgagcgcct gcatctggaa atggccgaac ggattctcgc gaaggctggt ataaatctgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat