SSGCID: Target Page

BrsuB.17333.a

DegT/DnrJ/EryC1/StrS family protein

CENTER ID:	BrsuB.17333.a
ORGANISM:	Brucella suis ATCC 23445
ASSOCIATED DISEASE:	Brucellosis
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BrsuB.17333.a.B1.GE38000	Full length( BrsuB.17333.a )		1	374

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	PATRIC_Locus_Tag:VBIBruSui83806_3247
BV-BRC:	fig\|470137.4.peg.3247
RefSeq:	YP_001628254.1
UniProt:	B0CI78

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQFIDLGAQR ARIENRLNAA ISKVVAEGRY ILGPEVAEFE KKLGEYLGVE HVIACANGTD ALQMPLMTRG IGPGHAVFVP SFTFAATAEV VALVGAEPVF VDVDPDSYNM NVEQLEAAIA ATIKEGRLEP KAIIPVDLFG LAASYNRITA IAEREGLFII EDAAQSIGGK RDNVMCGAFG HVGATSFYPA KPLGCYGDGG AMFTNDAELA DTLRSVLFHG KGETQYDNVR IGINSRLDTI QAAVLLEKLA ILEDEMEARD RIARRYNEAL KDVVKVPELP AGNRSAWAQY SIESENRDGL KAQLQAEGIP SVIYYVKPLH LQTAYKHYSV APGGLPVSES LPSRILSLPM HPYLSEADQD KILGVIRGFH GKKA

NT Sequence

ATGCAGTTCA TTGATCTTGG AGCGCAGCGC GCGCGTATCG AAAATCGTCT CAATGCCGCC ATTTCCAAGG TTGTTGCGGA AGGCCGTTAT ATTCTTGGGC CGGAAGTGGC TGAATTTGAA AAAAAGCTTG GCGAATATCT GGGTGTGGAG CATGTCATCG CCTGTGCCAA TGGCACTGAC GCCTTGCAGA TGCCCCTGAT GACACGCGGC ATCGGGCCGG GCCATGCGGT TTTTGTTCCG TCCTTCACCT TTGCAGCGAC CGCTGAGGTT GTTGCGCTCG TCGGTGCAGA GCCGGTATTC GTTGATGTCG ATCCAGATAG CTACAACATG AATGTCGAGC AGCTCGAAGC TGCCATTGCC GCCACCATCA AGGAAGGTCG TCTGGAGCCG AAGGCGATCA TTCCGGTCGA TCTTTTTGGC CTTGCCGCCT CTTACAATCG CATCACCGCC ATTGCCGAGC GCGAAGGCCT GTTCATCATC GAGGATGCGG CCCAGTCCAT CGGCGGCAAG CGCGACAATG TCATGTGCGG CGCTTTCGGC CATGTGGGCG CAACCAGCTT CTATCCCGCC AAGCCGCTTG GCTGCTACGG TGACGGTGGC GCCATGTTCA CCAATGATGC AGAATTGGCA GATACACTGC GCTCCGTGCT GTTCCACGGC AAGGGCGAGA CGCAGTATGA CAATGTCCGC ATCGGCATCA ATTCTCGCCT GGATACGATA CAGGCGGCTG TTCTTCTGGA AAAGCTTGCC ATTCTCGAAG ATGAGATGGA AGCCCGCGAC CGCATTGCAA GGCGCTATAA TGAGGCACTG AAGGATGTGG TGAAGGTGCC GGAACTTCCA GCCGGCAACC GCTCGGCCTG GGCGCAATAT TCTATCGAAA GCGAGAACCG CGACGGCTTG AAGGCACAAC TTCAGGCAGA AGGTATTCCG TCCGTCATCT ATTATGTGAA GCCTCTGCAC TTGCAGACGG CCTATAAGCA CTATTCCGTT GCCCCCGGTG GCCTGCCGGT TTCGGAAAGC CTGCCATCAC GCATTCTGAG CCTGCCGATG CATCCTTACC TTTCGGAAGC TGACCAGGAC AAGATTCTCG GCGTGATCCG TGGCTTCCAC GGCAAGAAGG CC

Details for BrsuB.17333.a.B1.GE38000

HARVESTED ON:	5/12/2014
SEQUENCED ON:	5/4/2014
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMQ FIDLGAQRAR IENRLNAAIS KVVAEGRYIL GPEVAEFEKK LGEYLGVEHV IACANGTDAL QMPLMTRGIG PGHAVFVPSF TFAATAEVVA LVGAEPVFVD VDPDSYNMNV EQLEAAIAAT IKEGRLEPKA IIPVDLFGLA ASYNRITAIA EREGLFIIED AAQSIGGKRD NVMCGAFGHV GATSFYPAKP LGCYGDGGAM FTNDAELADT LRSVLFHGKG ETQYDNVRIG INSRLDTIQA AVLLEKLAIL EDEMEARDRI ARRYNEALKD VVKVPELPAG NRSAWAQYSI ESENRDGLKA QLQAEGIPSV IYYVKPLHLQ TAYKHYSVAP GGLPVSESLP SRILSLPMHP YLSEADQDKI LGVIRGFHGK KA

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcagt tcattgatct tggagcgcag cgcgcgcgta tcgaaaatcg tctcaatgcc gccatttcca aggttgttgc ggaaggccgt tatattcttg ggccggaagt ggctgaattt gaaaaaaagc ttggcgaata tctgggtgtg gagcatgtca tcgcctgtgc caatggcact gacgccttgc agatgcccct gatgacacgc ggcatcgggc cgggccatgc ggtttttgtt ccgtccttca cctttgcagc gaccgctgag gttgttgcgc tcgtcggtgc agagccggta ttcgttgatg tcgatccaga tagctacaac atgaatgtcg agcagctcga agctgccatt gccgccacca tcaaggaagg tcgtctggag ccgaaggcga tcattccggt cgatcttttt ggccttgccg cctcttacaa tcgcatcacc gccattgccg agcgcgaagg cctgttcatc atcgaggatg cggcccagtc catcggcggc aagcgcgaca atgtcatgtg cggcgctttc ggccatgtgg gcgcaaccag cttctatccc gccaagccgc ttggctgcta cggtgacggt ggcgccatgt tcaccaatga tgcagaattg gcagatacac tgcgctccgt gctgttccac ggcaagggcg agacgcagta tgacaatgtc cgcatcggca tcaattctcg cctggatacg atacaggcgg ctgttcttct ggaaaagctt gccattctcg aagatgagat ggaagcccgc gaccgcattg caaggcgcta taatgaggca ctgaaggatg tggtgaaggt gccggaactt ccagccggca accgctcggc ctgggcgcaa tattctatcg aaagcgagaa ccgcgacggc ttgaaggcac aacttcaggc agaaggtatt ccgtccgtca tctattatgt gaagcctctg cacttgcaga cggcctataa gcactattcc gttgcccccg gtggcctgcc ggtttcggaa agcctgccat cacgcattct gagcctgccg atgcatcctt acctttcgga agctgaccag gacaagattc tcggcgtgat ccgtggcttc cacggcaaga aggcctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat